LOCUS       NM_001403593            6494 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus chromodomain helicase DNA binding protein 4 (Chd4),
            transcript variant 2, mRNA.
ACCESSION   NM_001403593 XM_036165722
VERSION     NM_001403593.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Clemot-Dupont,S., Lourenco Fernandes,J.A., Larrigan,S., Sun,X.,
            Medisetti,S., Stanley,R., El Hankouri,Z., Joshi,S.V.,
            Picketts,D.J., Shekhar,K. and Mattar,P.
  TITLE     The chromatin remodeler ADNP regulates neurodevelopmental disorder
            risk genes and neocortical neurogenesis
  JOURNAL   Proc Natl Acad Sci U S A 122 (3), e2405981122 (2025)
   PUBMED   39808658
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Shi,W., Wasson,L.K., Dorr,K.M., Robbe,Z.L., Wilczewski,C.M.,
            Hepperla,A.J., Davis,I.J., Seidman,C.E., Seidman,J.G. and
            Conlon,F.L.
  TITLE     CHD4 and SMYD1 repress common transcriptional programs in the
            developing heart
  JOURNAL   Development 151 (8) (2024)
   PUBMED   38619323
REFERENCE   3  (bases 1 to 6494)
  AUTHORS   Li,C., Wang,Z., Yao,L., Lin,X., Jian,Y., Li,Y., Zhang,J., Shao,J.,
            Tran,P.D., Hagman,J.R., Cao,M., Cong,Y., Li,H.Y., Goding,C.R.,
            Xu,Z.X., Liao,X., Miao,X. and Cui,R.
  TITLE     Mi-2beta promotes immune evasion in melanoma by activating EZH2
            methylation
  JOURNAL   Nat Commun 15 (1), 2163 (2024)
   PUBMED   38461299
  REMARK    GeneRIF: Mi-2beta promotes immune evasion in melanoma by activating
            EZH2 methylation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6494)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 6494)
  AUTHORS   Larrigan,S., Joshi,S.V. and Mattar,P.
  TITLE     Divergent phenotypes in constitutive versus conditional mutant
            mouse models of Sifrim-Hitz-Weiss syndrome
  JOURNAL   Hum Mol Genet 32 (24), 3361-3373 (2023)
   PUBMED   37738575
REFERENCE   6  (bases 1 to 6494)
  AUTHORS   McClive,P.J., Hurley,T.M., Sarraj,M.A., van den Bergen,J.A. and
            Sinclair,A.H.
  TITLE     Subtractive hybridisation screen identifies sexually dimorphic gene
            expression in the embryonic mouse gonad
  JOURNAL   Genesis 37 (2), 84-90 (2003)
   PUBMED   14595844
REFERENCE   7  (bases 1 to 6494)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 6494)
  AUTHORS   Loebel,D.A., O'Rourke,M.P., Steiner,K.A., Banyer,J. and Tam,P.P.
  TITLE     Isolation of differentially expressed genes from wild-type and
            Twist mutant mouse limb buds
  JOURNAL   Genesis 33 (3), 103-113 (2002)
   PUBMED   12124942
REFERENCE   9  (bases 1 to 6494)
  AUTHORS   Jones,D.O., Cowell,I.G. and Singh,P.B.
  TITLE     Mammalian chromodomain proteins: their role in genome organisation
            and expression
  JOURNAL   Bioessays 22 (2), 124-137 (2000)
   PUBMED   10655032
  REMARK    Review article
REFERENCE   10 (bases 1 to 6494)
  AUTHORS   Kim,J., Sif,S., Jones,B., Jackson,A., Koipally,J., Heller,E.,
            Winandy,S., Viel,A., Sawyer,A., Ikeda,T., Kingston,R. and
            Georgopoulos,K.
  TITLE     Ikaros DNA-binding proteins direct formation of chromatin
            remodeling complexes in lymphocytes
  JOURNAL   Immunity 10 (3), 345-355 (1999)
   PUBMED   10204490
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC134529.6 and AC166162.6.
            
            On Apr 1, 2022 this sequence version replaced XM_036165722.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223971.7208.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                AC134529.6         140208-140287
            81-259              AC134529.6         141114-141292
            260-381             AC134529.6         144495-144616
            382-576             AC134529.6         144826-145020
            577-695             AC134529.6         145245-145363
            696-937             AC134529.6         145480-145721
            938-1065            AC134529.6         145909-146036
            1066-1201           AC134529.6         146190-146325
            1202-1380           AC134529.6         146459-146637
            1381-1620           AC134529.6         146820-147059
            1621-1824           AC134529.6         148894-149097
            1825-2030           AC134529.6         149203-149408
            2031-2162           AC134529.6         150436-150567
            2163-2259           AC134529.6         150667-150763
            2260-2451           AC134529.6         151495-151686
            2452-2652           AC134529.6         152308-152508
            2653-2790           AC134529.6         152686-152823
            2791-2912           AC134529.6         153079-153200
            2913-3086           AC134529.6         153319-153492
            3087-3228           AC134529.6         153806-153947
            3229-3360           AC134529.6         154038-154169
            3361-3478           AC134529.6         154280-154397
            3479-3603           AC134529.6         157492-157616
            3604-3841           AC134529.6         157927-158164
            3842-4017           AC134529.6         158318-158493
            4018-4195           AC134529.6         164235-164412
            4196-4282           AC134529.6         164497-164583
            4283-4371           AC134529.6         164671-164759
            4372-4505           AC134529.6         164867-165000
            4506-4650           AC134529.6         165300-165444
            4651-4816           AC134529.6         165543-165708
            4817-4914           AC134529.6         165959-166056
            4915-5074           AC134529.6         166150-166309
            5075-5146           AC166162.6         95798-95869
            5147-5284           AC166162.6         96139-96276
            5285-5393           AC166162.6         96661-96769
            5394-5526           AC166162.6         96870-97002
            5527-5722           AC166162.6         102113-102308
            5723-6494           AC166162.6         103141-103912
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 59.28 cM"
     gene            1..6494
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /note="chromodomain helicase DNA binding protein 4"
                     /db_xref="GeneID:107932"
                     /db_xref="MGI:MGI:1344380"
     exon            1..80
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            81..259
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..5904
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /EC_number="3.6.4.12"
                     /note="isoform b is encoded by transcript variant 2;
                     CHD-4; Mi-2 beta"
                     /codon_start=1
                     /product="chromodomain-helicase-DNA-binding protein 4
                     isoform b"
                     /protein_id="NP_001390522.1"
                     /db_xref="GeneID:107932"
                     /db_xref="MGI:MGI:1344380"
                     /translation="MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLS
                     EAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSD
                     YTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVF
                     SEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGAS
                     VAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDA
                     KKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSR
                     SRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDP
                     DMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKD
                     GGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPS
                     PTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRN
                     YQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHS
                     VDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKL
                     KKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDT
                     ILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVT
                     YVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILG
                     SIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTP
                     ERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELS
                     PMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPN
                     GMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYER
                     IDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHND
                     IQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMS
                     KQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTE
                     LQGMNEYLSSFKVAQYVVREEEMGEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQED
                     LARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRR
                     PSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWL
                     VRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLI
                     RKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPA
                     EDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAP
                     AEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEE
                     EEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKK
                     TYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFL
                     ARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESM
                     AGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLAN
                     RAPEPPPQQVAQQQ"
     exon            260..381
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            382..576
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            577..695
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            696..937
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1065
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1201
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1380
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1620
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1824
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..2030
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            2031..2162
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            2163..2259
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            2260..2451
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            2452..2652
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            2653..2790
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            2791..2912
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            2913..3086
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            3087..3228
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            3229..3360
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            3361..3478
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            3479..3603
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            3604..3841
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            3842..4017
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            4018..4195
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            4196..4282
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            4283..4371
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            4372..4505
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            4506..4650
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            4651..4816
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            4817..4914
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            4915..5074
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            5075..5146
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            5147..5284
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            5285..5393
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            5394..5526
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            5527..5722
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     exon            5723..6494
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6472..6477
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /note="hexamer: AATAAA"
     polyA_site      6494
                     /gene="Chd4"
                     /gene_synonym="9530019N15Rik; D6Ertd380e; Mi-2beta;
                     mKIAA4075"
                     /note="major polyA site"
ORIGIN      
        1 ccccccttcg gccgcggcga ggaggagctg gagcgggagt gacaccggcg agacccagcg
       61 cgacctgagg cggctctagg tgactcgggc cagtgcagag ggccccaggc cgcaggcagg
      121 agcagctggg ccaattccct ggccggggaa cggaagggga tggcgtcggg cctgggctcc
      181 ccgtccccct gctcagcggg cagtgaggag gaggatatgg acgcacttct gaacaacagc
      241 ctgcccccac cccatccaga aaatgaggaa gacccagacg aggatttatc cgaagcagag
      301 actccaaagc tcaagaagaa gaaaaagcct aagaaacctc gagaccctaa aatccctaag
      361 agcaagcgcc aaaaaaagga gctgggggac agctctgggg aggggccaga gtttgtggag
      421 gaggaggaag aggtggctct gcgctcagac agtgagggca gcgactatac tcctggcaag
      481 aagaagaaaa agaagctcgg acctaagaaa gagaagaaga gcaaatccaa gcggaaggag
      541 gaggaggaag aggaggacga ggatgatgac tcgaaggaac ctaagtcatc agcccagctt
      601 ttagaagact ggggtatgga agacatcgac catgtgttct cagaggagga ttatcggacc
      661 ctcaccaact acaaggcctt cagccaattt gttagacccc tgattgctgc taaaaacccc
      721 aagattgctg tctccaagat gatgatggtc ttgggtgcaa agtggaggga attcagtacc
      781 aacaacccct tcaagggcag ctccggagca tcagtggcag ctgcggcggc ggcggctgtg
      841 gctgtggttg agagcatggt gacagctact gaagtcgccc ctccccctcc ccctgtggag
      901 gtgccgatcc gcaaggccaa gaccaaggag ggcaaaggtc ccaatgctcg gaggaagccg
      961 aaaggcagcc cgcgagtgcc tgacgccaag aagcccaaac ccaagaaagt agctcctctg
     1021 aagatcaagc tgggagggtt cggctccaag cggaagagat cctcgagtga ggacgacgat
     1081 ttagatgtag agtctgactt cgacgatgcc agtatcaaca gctattctgt ttcggatggt
     1141 tccaccagcc gcagtagtcg gagccgtaag aaactccgga ccgctaaaaa gaaaaagaaa
     1201 ggcgaggagg aggtgactgc tgtggatggt tatgagacag accaccagga ctattgcgag
     1261 gtgtgccagc aaggcggcga gatcatcctg tgtgatacct gtccccgagc ctaccatatg
     1321 gtgtgcctgg acccagacat ggagaaggcc ccggagggca agtggagctg tccgcactgt
     1381 gagaaggagg ggatccagtg ggaagctaag gaggacaatt ctgagggtga ggagattctg
     1441 gaagaagtcg ggggggaccc agaagaggag gatgaccatc acatggaatt ctgtcgcgtc
     1501 tgcaaggacg gcggggagct cctgtgctgt gacacatgcc cttcttccta ccacatccac
     1561 tgcctgaacc ccccgctgcc agagatccca aacggcgaat ggctctgtcc ccgctgtact
     1621 tgtccagctc ttaagggcaa agtgcagaag atcctaattt ggaagtgggg ccagccaccg
     1681 tctcccacac cagtgcctcg gcctccagat gctgacccta ataccccgtc gcccaagccc
     1741 ttggaggggc ggccagagag gcagttcttt gtgaaatggc aaggcatgtc ttactggcac
     1801 tgctcctggg tgtcagaact gcagttggag ctgcactgtc aggtgatgtt cagaaactac
     1861 cagcggaaga atgatatgga cgagccgcct tctggggact ttggtggaga tgaagagaag
     1921 agccggaagc gaaagaacaa ggaccctaag tttgcagaga tggaagagcg cttctaccgc
     1981 tatggaataa aaccggagtg gatgatgatc caccgcatcc tcaaccacag tgtggacaag
     2041 aagggccacg ttcactattt aatcaagtgg cgagacctgc cctatgacca ggcgtcctgg
     2101 gagagcgagg acgtggagat tcaggactac gacctgttca agcagagcta ctggaaccac
     2161 agggagttaa tgaggggtga agaaggacga cccggcaaga agcttaagaa ggtgaagcta
     2221 cggaaactgg aaaggcctcc ggagacccca actgttgatc ccaccgtgaa gtatgagcga
     2281 cagccggagt atctggatgc gacgggtgga accctgcatc cctaccaaat ggaaggctta
     2341 aactggctcc gcttctcctg ggctcaggga accgatacta tcttggctga tgagatgggc
     2401 cttgggaaaa ctgtgcagac agcagtcttc ctctactccc tctataagga gggtcactcc
     2461 aaaggcccct tcttagtgag cgctcctctg tccaccatca tcaactggga gagagagttt
     2521 gagatgtggg ctcctgatat gtatgtggta acctatgtgg gtgacaagga cagccgtgcc
     2581 atcatccggg aaaatgagtt ctcctttgaa gacaatgcca ttcgtggtgg caagaaggcc
     2641 tcgaggatga agaaagaagc atcggtgaaa ttccatgttc tgctgacatc ctatgagttg
     2701 atcaccattg atatggccat cttgggttct attgactggg cctgcctcat tgtggatgaa
     2761 gcccatcggc tgaagaacaa ccagtctaag ttctttcgag ttttgaatgg ttactcactc
     2821 cagcacaagc tgttgctaac tggaactccg ttacagaaca atctagagga actgtttcat
     2881 ctgctcaact ttctcacccc tgagaggttc cacaacttag aaggcttctt ggaggagttt
     2941 gcagacattg ccaaagagga ccagattaaa aaactccacg acatgctggg gcctcatatg
     3001 ttgcggcggc tcaaagctga cgtcttcaag aatatgccat ccaagacaga gctgattgtg
     3061 cgcgtggagt tgagtcctat gcagaagaag tactacaagt atattctcac gcggaatttc
     3121 gaagcactta atgctcgagg tggtggcaac caggtttctc tgctgaacgt ggtgatggat
     3181 cttaagaaat gctgcaacca cccttatctc ttccctgtgg cagcaatgga agcccctaag
     3241 atgcctaatg gtatgtatga tggcagtgcc ctaatcagag catctgggaa gttgttgctg
     3301 ctgcagaaga tgcttaagaa cctgaaagag ggagggcatc gtgtgctcat cttctcccag
     3361 atgaccaaga tgttggactt gctagaggat tttttagaac atgaaggtta taaatatgaa
     3421 cgtattgatg gtggaatcac tgggaacatg cgtcaggagg ccattgatcg cttcaatgca
     3481 ccgggtgctc aacagttctg cttcttgctt tccactcgag ctgggggcct tgggatcaat
     3541 ctggccactg cggacacagt tattatatat gactctgact ggaaccccca taatgacatc
     3601 caggccttta gcagagccca ccgtattggg caaaataaga aggtgatgat ctaccggttt
     3661 gtgacccgtg catcagtgga ggagcgcatc acgcaggtgg caaagaagaa gatgatgctg
     3721 acacatttag tggtacggcc tggcctgggc tccaagacag gctcaatgtc taaacaggag
     3781 cttgatgata tcctcaaatt tggcactgag gagctattca aggatgaagc cacggatgga
     3841 ggaggagaca acaaggaggg agaagacagc agtgttatcc actatgacga taaggccatt
     3901 gaacgactgc tggatcgaaa ccaggatgag actgaagaca cagaattgca gggcatgaat
     3961 gaatatttga gctcattcaa agtggctcag tatgtggtac gggaagaaga gatgggggaa
     4021 gaggaggtag aacgggaaat cataaaacag gaagaaagtg tggatcctga ctactgggag
     4081 aaattgctgc ggcaccatta tgagcagcag caagaagatc tagcccgaaa tctgggcaaa
     4141 ggaaaaagaa tccgtaaaca ggtcaactac aatgatggct cacaggagga ccgagattgg
     4201 caggacgacc agtccgacaa ccagtccgat tactcagtgg cctcagagga aggtgatgaa
     4261 gactttgacg aacggtcaga agctccccgc aggcccagtc gcaagggcct gcggaatgat
     4321 aaagataagc cattacctcc tctgttggcc cgtgttggtg ggaatattga agtacttggt
     4381 tttaatgctc gtcagcgaaa agcatttctt aatgcaatta tgcgatatgg catgccacct
     4441 caggatgctt ttaccactca gtggcttgtg agagatcttc gaggcaagtc agagaaagaa
     4501 tttaaggctt atgtgtcact cttcatgcga catttgtgtg agcctggggc agatggggct
     4561 gagacctttg ctgatggcgt cccacgagaa ggcctgtctc gtcaacatgt tctcactagg
     4621 atcggtgtca tgtctctgat tcgaaagaag gttcaggagt ttgaacatgt taatggacgc
     4681 tggagcatgc ccgaactggc tgaagtagag gaaaacaaaa aaatgtctca gccaggttca
     4741 ccttctccaa agactcctac accttccaca ccaggggaca cacaacccaa tactcctgcg
     4801 cctgtgccac ctgctgagga tgggataaaa atagaggaaa atagccttaa agaagaagag
     4861 agcacagaag gagagaagga agttaaatct acagcccctg aggcgactgt ggagtgtgca
     4921 cagccccctg cccctgcccc tgccactgcc cctgccactg ccactgcccc tgaggatgac
     4981 aaggctcctg ctgagcctcc tgagggggag gaaaaagtgg aaaaggcaga ggtgaaggag
     5041 agaacagagg agcctatgga gacagaggcc aaaggtacta ctgaggtgga gaaagtggag
     5101 gagaagtctg cggtagacct gaccccgatc gtggtggaag acaaagaaga gaagaaggag
     5161 gaggaggaga aaaaggacgt gatgcttcag aatggagaaa ctcccaagga tcttagtgat
     5221 gagaagcaga agaaaaacag caaacagcgt ttcatgttca acattgcaga tggtggtttt
     5281 actgagttgc actcactgtg gcagaatgaa gagcgggcag ccacagtcac taagaagact
     5341 tacgagatct ggcaccgtcg ccatgattac tggctgctgg ctggcattat aaaccacggc
     5401 tatgcccggt ggcaggacat ccagaatgac ccacggtatg ccatcctcaa tgagcctttc
     5461 aagggtgaaa tgaatcgtgg caattttcta gagatcaaga ataaattttt agctcgaaga
     5521 ttcaagctct tagaacaagc cctggtgatc gaggagcagc ttcgcagggc agcttacctg
     5581 aacatgtccg aggacccctc tcacccgtct atggccctta acacccgctt cgctgaggtg
     5641 gagtgtttgg cagaaagtca ccagcacctg tccaaggagt cgatggcagg gaataagccg
     5701 gccaatgcag tcctacacaa agtcctgaaa cagctggagg aactgttgag tgacatgaaa
     5761 gctgatgtga cgcggctccc agccaccatt gcccgcatcc ccccagtcgc tgtgaggtta
     5821 cagatgtcag agcggaacat tctcagccgc ctggcaaaca gggcgccaga acctccccca
     5881 cagcaggtag cccagcagca gtgaaagacc cagagtgata tccacctcca ccactcagca
     5941 gtgaccttcc tcactttctc ttgtcccggc tgcccctggg ggccagagag acccttgcct
     6001 tccttctgcc catcttctga gttgtaaagg aacagccctg cgcagtgggg cagggaggga
     6061 gggagaagca atggtgccct tcctgctgga gagacaagca gcagtagcgc agcgcccgcc
     6121 cgccatgtgc aggagctggc gggctggctg ccttctggac cctggcttct ccccaccgtg
     6181 cgcctgttac aaactgttgt ggattctgcc aggctcgaag aaaatgatct gaatttcttc
     6241 ctctttttgg ttttattttg ttggtttact ttgtgttttc tcctttttgg ggggtattca
     6301 gagtgggccg ggcccctggg cgagacacag ctacctctgt tggcatcttt ttaataccag
     6361 gaacccagcg gctccagcca ctgagcagct aaataaagtt ggaaaaaaaa aaaaaaagaa
     6421 aaagaaccaa aagcataaaa aaccacagca aatttcttga tgagaactga aaataaaagt
     6481 ttccttgtat ttta
//
