LOCUS       NM_001081345            9071 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus chromodomain helicase DNA binding protein 2 (Chd2),
            mRNA.
ACCESSION   NM_001081345 XM_897304
VERSION     NM_001081345.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 9071)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9071)
  AUTHORS   Wilde,J.J., Aida,T., Del Rosario,R.C.H., Kaiser,T., Qi,P.,
            Wienisch,M., Zhang,Q., Colvin,S. and Feng,G.
  TITLE     Efficient embryonic homozygous gene conversion via RAD51-enhanced
            interhomolog repair
  JOURNAL   Cell 184 (12), 3267-3280 (2021)
   PUBMED   34043941
REFERENCE   4  (bases 1 to 9071)
  AUTHORS   Rom,A., Melamed,L., Gil,N., Goldrich,M.J., Kadir,R., Golan,M.,
            Biton,I., Perry,R.B. and Ulitsky,I.
  TITLE     Regulation of CHD2 expression by the Chaserr long noncoding RNA
            gene is essential for viability
  JOURNAL   Nat Commun 10 (1), 5092 (2019)
   PUBMED   31704914
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9071)
  AUTHORS   Ikegami,K., Saigoh,K., Fujioka,A., Nagano,M., Kitajima,K., Sato,C.,
            Masubuchi,S., Kusunoki,S. and Shigeyoshi,Y.
  TITLE     Effect of expression alteration in flanking genes on phenotypes of
            St8sia2-deficient mice
  JOURNAL   Sci Rep 9 (1), 13634 (2019)
   PUBMED   31541165
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9071)
  AUTHORS   Nagarajan,P., Onami,T.M., Rajagopalan,S., Kania,S., Donnell,R. and
            Venkatachalam,S.
  TITLE     Role of chromodomain helicase DNA-binding protein 2 in DNA damage
            response signaling and tumorigenesis
  JOURNAL   Oncogene 28 (8), 1053-1062 (2009)
   PUBMED   19137022
  REMARK    GeneRIF: Chd2 heterozygous mutant mice exhibit increased
            extramedullary hematopoiesis and susceptibility to lymphomas.
REFERENCE   7  (bases 1 to 9071)
  AUTHORS   Kulkarni,S., Nagarajan,P., Wall,J., Donovan,D.J., Donell,R.L.,
            Ligon,A.H., Venkatachalam,S. and Quade,B.J.
  TITLE     Disruption of chromodomain helicase DNA binding protein 2 (CHD2)
            causes scoliosis
  JOURNAL   Am J Med Genet A 146A (9), 1117-1127 (2008)
   PUBMED   18386809
REFERENCE   8  (bases 1 to 9071)
  AUTHORS   Marfella,C.G., Ohkawa,Y., Coles,A.H., Garlick,D.S., Jones,S.N. and
            Imbalzano,A.N.
  TITLE     Mutation of the SNF2 family member Chd2 affects mouse development
            and survival
  JOURNAL   J Cell Physiol 209 (1), 162-171 (2006)
   PUBMED   16810678
  REMARK    GeneRIF: Our results demonstrate that mutation of Chd2 dramatically
            affects mammalian development and long-term survival.
            Erratum:[J Cell Physiol. 2007 Aug;212(2):562]
REFERENCE   9  (bases 1 to 9071)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 9071)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC099699.16 and AC154883.13.
            
            On Jun 6, 2008 this sequence version replaced NM_001081345.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-501               AC099699.16        170646-171146
            502-634             AC099699.16        171488-171620
            635-866             AC099699.16        192684-192915
            867-953             AC154883.13        210253-210339       c
            954-1015            AC154883.13        208617-208678       c
            1016-1123           AC154883.13        202333-202440       c
            1124-1264           AC154883.13        199515-199655       c
            1265-1398           AC154883.13        197908-198041       c
            1399-1624           AC154883.13        196436-196661       c
            1625-1725           AC154883.13        195462-195562       c
            1726-1770           AC154883.13        194466-194510       c
            1771-1949           AC154883.13        194121-194299       c
            1950-2074           AC154883.13        192234-192358       c
            2075-2291           AC154883.13        187952-188168       c
            2292-2381           AC154883.13        185862-185951       c
            2382-2572           AC154883.13        185006-185196       c
            2573-2761           AC154883.13        178936-179124       c
            2762-2924           AC154883.13        175488-175650       c
            2925-3077           AC154883.13        174928-175080       c
            3078-3149           AC154883.13        173186-173257       c
            3150-3299           AC154883.13        169871-170020       c
            3300-3448           AC154883.13        169033-169181       c
            3449-3545           AC154883.13        167539-167635       c
            3546-3638           AC154883.13        166324-166416       c
            3639-3809           AC154883.13        164085-164255       c
            3810-3985           AC154883.13        162983-163158       c
            3986-4027           AC154883.13        158875-158916       c
            4028-4167           AC154883.13        158163-158302       c
            4168-4306           AC154883.13        150301-150439       c
            4307-4457           AC154883.13        150018-150168       c
            4458-4580           AC154883.13        148856-148978       c
            4581-4709           AC154883.13        147626-147754       c
            4710-4850           AC154883.13        146226-146366       c
            4851-4985           AC154883.13        143684-143818       c
            4986-5164           AC154883.13        141662-141840       c
            5165-5264           AC154883.13        138762-138861       c
            5265-5478           AC154883.13        136113-136326       c
            5479-5722           AC154883.13        130262-130505       c
            5723-9071           AC154883.13        121179-124527       c
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 41.85 cM"
     gene            1..9071
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /note="chromodomain helicase DNA binding protein 2"
                     /db_xref="GeneID:244059"
                     /db_xref="MGI:MGI:2448567"
     exon            1..501
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            502..634
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             573..6056
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /EC_number="3.6.4.12"
                     /note="ATP-dependent helicase CHD2"
                     /codon_start=1
                     /product="chromodomain-helicase-DNA-binding protein 2"
                     /protein_id="NP_001074814.2"
                     /db_xref="CCDS:CCDS52274.1"
                     /db_xref="GeneID:244059"
                     /db_xref="MGI:MGI:2448567"
                     /translation="MMRNKDKSQEEDSSLHSNASSRSASEEVSGSDSGSQSESEQGSE
                     PGSGHGSESNSSSESSESQSESESESAGSKSQPVLPEAKEKPASKKERIADVKKMWEE
                     YPDVYGVRRSNRSRQEPSRFNVKEEASSGSESGSPKRRGQRQLKKQEKWKQDPSEDEQ
                     EQGTSAESEAEQKKGKARRPVPRRTVPKPQVKKQPKIQRGKRKKQESSDDDDDDDEAP
                     KRQTRRRAAKNVSYKEDDDFETDSDDLIEMTGEGGDEQQDNSETIEKVLDSRLGKKGA
                     TGASTTVYAVEANGDPSDDFDTEREEGEVQYLIKWKGWSYIHSTWESEDSLQQQKVKG
                     LKKLENFKKKEDEVKQWLGKVSPEDVEYFSCQQELASELNKQYQIVERVIAVKTSKST
                     LGQTDFPAHSRKPAPSNEPEYLCKWMGLPYSECSWEDEALIGKKFQNCIDSFHSRNNS
                     KTIPTRECKALKQRPRFVALKKQPAYLGGESLELRDYQLEGLNWLAHSWCKSNSVILA
                     DEMGLGKTIQTISFLSYLFHQHQLYGPFLIVVPLSTLTSWQREFEIWAPEINVVVYIG
                     DLMSRNTIREYEWIHSQTKRLKFNALITTYEILLKDKTVLGSINWAFLGVDEAHRLKN
                     DDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFEFWEDFEEDHGKGR
                     ENGYQSLHKVLEPFLLRRVKKDVEKSLPAKVEQILRVEMSALQKQYYKWILTRNYKAL
                     AKGTRGSTSGFLNIVMELKKCCNHCYLIKAPEDSERESGQEVLQSLIRSSGKLILLDK
                     LLTRLRERGNRVLIFSQMVRMLDILAEYLTIKHYPFQRLDGSIKGEIRKQALDHFNAD
                     GSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYR
                     LVTKGTVEEEIIERAKKKMVLDHLVIQRMDTTGRTVLENNSGRSNSNPFNKEELTAIL
                     KFGAEDLFKEIEGEESEPQEMDIDEILRLAETRENEVSTSATDELLSQFKVANFATME
                     DEEELEERPHKDWDEIIPEEQRKKVEEEERQKELEEIYMLPRIRSSTKKAQTNDSDSD
                     TESKRQAQRSSASESETDDSDDDKKPKRRGRPRSVRKDLVEGFTDAEIRRFIKAYKKF
                     GLPLERLECIARDAELVDKSVADLKRLGELIHNSCVSAMQEYEEQLKESTSEGKGPGK
                     RRGPTIKISGVQVNVKSIIQHEEEFEMLHKSIPVDPEEKKKYCLTCRVKAAHFDVEWG
                     VEDDSRLLLGIYEHGYGNWELIKTDPELKLTDKILPVETDKKPQGKQLQTRVDYLLKL
                     LRKGLEKKGTVASGEEAKLKKRKPRVKKENKAPRLKDEHGLEPASPRHSDNPSEEGEV
                     KDDGLEKSPTKKKQKKKENKENKEKPVSSRKDREGDKERKKSKDKKEKVKGGDGKSSS
                     KSKRSQGPVHITAGSEPVPIGEDEDDDLDQETFSICKERMRPVKKALKQLDKPDKGLS
                     VQEQLEHTRNCLLKIGDRIAECLKAYSDQEHIKLWRRNLWIFVSKFTEFDARKLHKLY
                     KMAHKKRSQEEEEQKKKDDSLGGKKPFRPEASGSSRDSLISQSHTSHNLHPQKPHLPA
                     SHGPQMHGHPRDNYSHPNKRHFSNADRGDWQRERKFNYGGGNSAPWGGDRHHQYEQHW
                     YKDHHYGDRRHMDAHRSGSYRPNNMSRKRPYEQYNSDRDHRGHRDYYDRHHHDSKRRR
                     SDDFRPQNYHQQDFRRMSDHRPTMGYHGQGPSDHYRSFHTDKLGEYKQPMPSLHTALS
                     DPRSPPSQKSPHDSKSPLDHRSPLERSLEQKNNPDYNWNVRKT"
     exon            635..866
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            867..953
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1015
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1123
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1264
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1398
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1624
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1725
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1770
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1949
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2074
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2075..2291
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2381
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2382..2572
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2573..2761
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2762..2924
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2925..3077
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3078..3149
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3150..3299
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3300..3448
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3449..3545
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3546..3638
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3639..3809
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3810..3985
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3986..4027
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4028..4167
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4168..4306
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4307..4457
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4458..4580
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4581..4709
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4710..4850
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4851..4985
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4986..5164
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5165..5264
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5265..5478
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5479..5722
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5723..9071
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9006..9011
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /note="hexamer: ATTAAA"
     polyA_site      9029
                     /gene="Chd2"
                     /gene_synonym="2810013C04Rik; 2810040A01Rik;
                     5630401D06Rik; CHD-2"
                     /note="major polyA site"
ORIGIN      
        1 agagctggga aggaggctct agatggcggc tgtgccttag agagagcgcg ctttgctccc
       61 tgcttcggtt cactttacgc aactttccct aactttcggg cagcctcagg gggcccccgc
      121 agccccctgc ctcttctagt gacttactgg ggtcgatccg aacctttttt tgggagaaaa
      181 gctgctttta ggagctttct tttcgtgcct tgttggaaga agctgccgta ttgagagccc
      241 aggtcgttgt tttttccagc ttagaagcca tggcgcacct ccatttttgt gcgctctcct
      301 aatgaggttt ttttttcccc ttccggacct gttttagtat taattattgc tttatttttt
      361 gacaagtcaa catatttgag gattatttca tttacttttt gttttttaac ggagaatttt
      421 gcgtttttaa aaattatttt ggatcggata tttttctact actagatagg actcttgctt
      481 tggacctact acatggatca gtaaatacct gggcacagga cttcaaagca aacacagatt
      541 ccccctcccc cttaatattt aagaattaaa agatgatgag aaataaggac aaaagccaag
      601 aggaggacag ttcgctacac agcaatgcat cgagtcgttc agcttctgaa gaagtgtcgg
      661 ggtcagactc aggcagtcag tcggaaagtg agcagggcag cgagcccgga agtggacatg
      721 gcagcgagtc caatagcagt tcggagtctt ctgagagcca atcagagtct gagagtgaat
      781 cggcaggttc caaatcccag ccagtccttc cggaagccaa agagaagcct gcctctaaga
      841 aggaacggat agctgatgtg aagaagatgt gggaagaata tcctgatgtt tatggggtta
      901 ggaggtcaaa tcgaagcaga caagaaccat cacgatttaa tgttaaggaa gaggcaagta
      961 gcgggtctga gagtgggagt ccgaaaagaa gaggccagag gcagctgaaa aaacaagaaa
     1021 aatggaaaca ggacccctca gaagacgaac aggaacaggg aaccagtgca gagagtgagg
     1081 cagaacaaaa gaaaggaaaa gctagaagac cggtccctag aagaacagtg cccaaacctc
     1141 aagttaaaaa gcaacctaag attcagcgtg gaaagaggaa aaaacaagag tcttcagatg
     1201 acgacgacga tgatgatgaa gctcccaaaa ggcagactcg ccgaagagct gctaaaaacg
     1261 tgagttacaa agaagatgat gactttgaga ctgattcaga tgatctcatt gagatgactg
     1321 gagaaggagg tgatgaacag caagataata gtgaaactat tgaaaaggtc ttagattcaa
     1381 gactgggaaa gaaaggagcc actggagcgt ctaccactgt gtatgctgtt gaagctaatg
     1441 gagatcctag cgatgacttt gacacggaga gggaggaagg tgaagtccag taccttatca
     1501 agtggaaggg ctggtcttat attcacagca catgggagag tgaagactcc ttacagcaac
     1561 agaaagtgaa gggcctaaag aaactggaga atttcaagaa gaaagaggat gaggtcaagc
     1621 aatggctagg gaaagtttct cctgaagatg ttgaatattt cagttgccaa caggagctgg
     1681 catcagagtt gaataagcag tatcagatag tagagagagt aatagctgtg aagacaagta
     1741 aatctacact gggccaaaca gatttcccag ctcacagtcg gaagccagca ccttcaaatg
     1801 agcctgagta tctgtgcaag tggatgggac taccctactc agagtgcagc tgggaagatg
     1861 aagccttgat tggaaagaaa ttccagaatt gtatcgacag cttccacagt cgaaacaact
     1921 ccaaaaccat tcccacaaga gaatgcaagg ctctaaagca aaggccgaga tttgtggctt
     1981 tgaagaagca gccagcttat ttaggagggg agagtctgga gctccgagac tatcagctgg
     2041 agggcctcaa ctggctggct cactcctggt gcaaaagtaa cagtgtaatc cttgctgatg
     2101 aaatgggcct aggaaagacc atccagacca tatcgtttct ctcctacctg ttccaccagc
     2161 accagctcta tgggcctttt cttatagttg ttcccttgtc cacccttacc tcatggcaga
     2221 gagagtttga gatctgggca ccagagatta atgtcgtggt ttacataggt gacctgatga
     2281 gcagaaatac gataagggaa tatgagtgga ttcattctca aaccaaaagg ttgaagttca
     2341 atgcacttat aacaacatat gagatcctcc taaaagataa gaccgtgctg ggcagtatta
     2401 actgggcctt cctgggagta gatgaagccc atcggttgaa gaatgatgac tctttattgt
     2461 ataaaactct gattgatttc aagtccaacc ataggctact gatcacgggc acccctcttc
     2521 agaattccct caaggaactt tggtccttgc tgcactttat tatgccggag aagtttgagt
     2581 tctgggaaga ttttgaagaa gaccatggaa aagggagaga gaatggctac cagagtcttc
     2641 ataaggtact agagcctttt cttcttcgaa gagtcaaaaa agatgtggag aaatcccttc
     2701 ctgccaaagt ggagcagatt ctcagggtgg agatgtcagc ccttcagaaa cagtattata
     2761 aatggatcct gaccaggaat tacaaggctc ttgccaaagg gaccagaggc agcacatctg
     2821 gcttccttaa tatcgtgatg gagctgaaaa agtgctgcaa ccactgttat ctgatcaaag
     2881 ctcccgagga cagcgagagg gagagcggcc aggaggtcct tcagtctcta attaggagca
     2941 gtgggaagct gatcctgtta gacaaactgt tgacaagact ccgagaaagg ggaaacagag
     3001 tccttatctt ctctcagatg gtgaggatgt tggatatcct ggccgagtac ctgactatca
     3061 aacactatcc tttccagcgc ctggatggtt ctatcaaggg ggaaatccga aagcaggcac
     3121 tggatcactt caatgcagat gggtctgagg acttctgttt cctgctctcc acacgggctg
     3181 gcggcctggg aatcaatttg gcttcagcgg acacagtcgt catctttgac tctgactgga
     3241 acccgcagaa cgacttgcag gcacaagccc gagcacatag aatcggtcag aagaagcagg
     3301 tgaatattta ccgcctggtt acaaaaggga ctgtggaaga ggagatcata gaacgggcca
     3361 aaaagaagat ggtgttggac catctggtga ttcagcgcat ggataccact ggcaggacgg
     3421 ttcttgaaaa caactcagga agatccaact caaacccttt taataaagaa gagctgacag
     3481 ctattttgaa atttggagca gaggatctct tcaaagaaat tgaaggagag gagtcagagc
     3541 ctcaggaaat ggatatagat gaaattttgc gtttagctga aaccagagag aatgaagtgt
     3601 caactagtgc gacagatgag ctgctctcac agtttaaggt tgccaacttt gcaacaatgg
     3661 aagatgaaga ggagctagaa gagcgtcctc acaaagactg ggatgaaatt attcccgaag
     3721 agcaaaggaa aaaagtggag gaggaagaac ggcagaagga gctggaagaa atttatatgc
     3781 tgcctcggat tcgcagttcc actaaaaagg ctcagacaaa cgacagtgat tcagatactg
     3841 agtctaagag gcaggcccag aggtcttctg cttctgagag tgagacggac gactctgatg
     3901 atgacaagaa gccgaagcga agagggcgcc cccgaagtgt gcgcaaagac cttgtggagg
     3961 gcttcacgga tgccgagatc agaaggttca tcaaggctta taagaagttt ggtctccctc
     4021 ttgaaaggct agagtgcata gcacgtgatg ctgagctagt tgacaagtct gtggcagact
     4081 tgaaacgcct gggggagctg attcataata gctgtgtgtc agcaatgcag gaatatgagg
     4141 agcaactgaa agaaagcacc agtgaaggga aaggaccagg caagagaaga ggcccaacaa
     4201 tcaagatctc tggggttcag gtgaacgtga agtccattat ccaacacgaa gaagagtttg
     4261 agatgctgca taaatctatc cctgtggacc ctgaggaaaa aaaaaagtac tgcctaacct
     4321 gccgagtcaa agcagcacat tttgacgtcg agtggggcgt ggaggacgat tctcgtctgt
     4381 tgcttggaat ctatgaacat ggctatggaa attgggagct aattaagaca gacccagagc
     4441 taaagctaac tgataaaatt ctaccagtgg agacggataa aaagccgcag gggaagcagc
     4501 tgcagacccg agtggactac ttgctgaagc tgctcaggaa gggtctggag aagaagggca
     4561 ccgtggccag cggagaggag gccaaactaa agaagcggaa gcctcgagta aagaaggaga
     4621 acaaagcccc caggctgaag gacgagcacg ggctggagcc agcgtcgcct cgacactccg
     4681 acaacccatc tgaagagggg gaagtgaagg atgatggtct agaaaaaagt ccaacgaaaa
     4741 aaaagcagaa gaagaaagag aacaaggaga acaaagagaa accagtgagc tctcggaagg
     4801 acagggaagg agacaaagag aggaagaagt ccaaagacaa gaaagagaag gtcaaaggtg
     4861 gtgatggcaa gtcttcaagt aaatcaaagc gatctcaggg tcctgtgcac attacagcag
     4921 gaagtgagcc tgttcccatc ggggaggatg aagacgatga tctggaccag gaaaccttca
     4981 gcatttgtaa ggagaggatg aggcctgtga agaaggcact gaaacagttg gacaaacctg
     5041 acaaagggct cagtgtgcaa gaacagctgg aacacaccag gaactgcctg ctcaaaattg
     5101 gagaccgcat agccgagtgc cttaaagctt actcagacca ggagcacatc aaattgtgga
     5161 ggaggaacct atggattttt gtttccaagt ttacagaatt tgatgcacga aaactacata
     5221 agttatataa gatggctcat aagaaaagat ctcaagaaga ggaggaacaa aagaagaaag
     5281 atgattcgct tggtggtaag aaaccatttc gaccagaggc ctcgggctca agccgggatt
     5341 ctctgatatc tcagtcccat acctcacaca accttcatcc tcagaagcct catttgcctg
     5401 cctcccatgg cccacaaatg catggacatc caagagataa ctacagtcac cccaacaaga
     5461 gacactttag taacgcagat cgaggagact ggcagaggga aagaaagttc aactatggtg
     5521 gtggtaacag cgccccctgg gggggcgacc ggcaccatca gtatgagcag cattggtata
     5581 aggaccacca ctatggtgac cggaggcata tggatgccca ccgttctggg agctaccgcc
     5641 ctaacaacat gtccagaaag aggccgtatg agcagtacaa cagcgaccga gaccaccggg
     5701 gacacagaga ctattatgac aggcaccatc atgactccaa gcggaggagg tcagatgact
     5761 tcaggcccca gaattatcac cagcaggatt tccgacgaat gtctgaccac agacccacca
     5821 tgggttacca cggccaggga ccctcagacc attatcgctc tttccacaca gacaagttgg
     5881 gggaatataa gcagcccatg ccttcattgc acactgcgct ctcagaccca cgctcacccc
     5941 cttcccagaa atctccccat gattccaaat cacccttgga tcataggtct cctttggaga
     6001 gatcactaga acagaaaaac aacccagatt ataactggaa tgttcggaaa acataaagga
     6061 ccactcctga agacagggag atcgcggaag tcatcaagca cttgggcgtt ctgctccgat
     6121 gcagctgtgg ccagcggtca ggctgctgag ccgggtttct ggatatgctg ctgctgttga
     6181 atccactgac ttggcagctg aaggagcact tgaaggagtc ggaggccttc gtctgctgct
     6241 ttggttcggt ctccactcct gcactttggc cccacccacc catcctagcc tctcatggcc
     6301 tcccaccccc atgggtgcta ggaggaggtg actttatata ccagcttcat tgggctgctt
     6361 cctctgaagg aatccttgag agtcagtgaa tgttctatca gcatgagtga ccagggcaag
     6421 gacctgctgg acagtgtgtg attggttcac catgacacga gggacgatgc tcatgggaag
     6481 tgggggcaca gggtgggttt cacactgcaa gacttgaaag ggggacaggt atacccctta
     6541 gcccttagct gtgttattgg gagaaatgat acacttggtc tcattatgtg aagcggggtg
     6601 gggataacga gacagggaaa tggccgccat ggtggcctgc tccctgccaa cactaatgtt
     6661 ccccacacta tctttgtaca tttcagaggt ttggtcttct gggtggtgtc cgtttcccct
     6721 cactgtcagg gaaagctgga tccctctggc tgacttagag tactgtgact tgggcattgc
     6781 taatgccagc gtggggcttg cttgtttggg gagtgggcta agtgggtgta ggggtggaga
     6841 tgggggtggg gttattatag ccaggaaaat aggaatccct ggtgggtttc tgattcctgt
     6901 gctgagaagg agagtcacag gtcctggaga tagaaatgga ggtggcctgc tgataagata
     6961 cctgctggtg agccctgatg tgggacatac gtgcagatag ggggcagggt tcgtttacag
     7021 tgccagcaag catggagttg tcagtattgg agtgagttgt cgaattgtag ctgcatcttg
     7081 gttcctttac acagcttgaa ggggcagcct gcacagactg aaatatcttg acagttgaga
     7141 tgtggtcagg tgaaaagcta catctccatt acaagttgga actcagtaga atcaaacctt
     7201 gttactttaa gatctgtaga aataaaccta agttaatttt cccccaaatc acgagcatcc
     7261 gtgatggggt agccaagctg ttaaggtggc aggaggctgg tcagcgtcgg gttgggttgg
     7321 gttgggttgg gttgtgggac agatggattt tatcattgaa tggctcttct cagtgtatgg
     7381 atactgggtt gggccttggg ttttactcac tttcagtata gcttgccagt attgttaagg
     7441 tatccaaagc ctttctagat agagacagga acattgactt gaacatatgt atgtaagagt
     7501 ccaggctcat tctgagttga tgaaaagtct tgtttggact tctgcagggt ttgactccct
     7561 ggacactctg agtggggtgg tcatccaggc agtactatta atgacctccc cagggcaggc
     7621 atgagttgtg tcctgagtgg aacatctggc catgcatggt tgtgatgggc cattgctttc
     7681 gctgctttag agcaccacag acctgactgc tgccgagtcg gtccctgcag ctaatgctcg
     7741 aagaccttct gggctcctcg tggcactagg cccttcacct tgactgtgtg catgcatgac
     7801 ctgcaataga gcccaccaac cacatagaag tcccctggct agagtctgat gcctggtggc
     7861 ttaccggact tctcagtcaa ttctgaaagg tatattttgg gcacacagtg aaaagtgagc
     7921 catagatgga gcttcttgct gatcacattg acactgtctg tgtttgattt gctgagctct
     7981 tggatgttgg gagtgtagca ggcacaggag gtacctctgc agaaggaagg cggagggtgg
     8041 aggccttgcc tttccatatt cacctaggtc ttgtctttca acttgttggt agaagttaca
     8101 aattattctc aaaactgtct gtctcatcat gaggaaaaag gaactttctt ttgttcctat
     8161 tcaggacttg aggtatagtg ccataaaatg tagcaaaaac cctatcggcc agatcagtgt
     8221 tacattgatt ctaagattac agtatgtccc atcaaacatc tgctgtaggt gagggagtgg
     8281 atttgaagag gggttgaaag aaaggaagca ttcctcttgc tttaaactag tatgtgcatc
     8341 agatgcttac agcagaagtt tgcacaggta agagaagctg ccagtgacgt tctgtggggc
     8401 ctggctttgg gcagctgctc atcattgagg aagtggcagg acttggagga agttatgtgt
     8461 gaatgttgtc aagtcctgaa gtcctatgat gcactgtggc cctagaaaac gaagatgaag
     8521 cctccactga ccactgtgtc ctctctgact tggccagcct gatggtcctt ccttctactc
     8581 tgtgtagtca ttcctaagcc ccacagaggg agcgccaaca tttctcactg tgctgctgag
     8641 gttctgacct ggtgtgtctg acattgcatc aggctttgca gaacaaatgt gattcactta
     8701 aacgttcaca cacagcaaca ctgaaatcct acagaccaaa caaacccact tgtcagcagg
     8761 agccgtggtc cagcccagca cctgtgctct cgtagccgag gccagccgtg ttgggaagag
     8821 agccatgcca agggtccagg ctgctgtagt ccacctgtgc cctactcatt tggggacagg
     8881 tggtttttct tgtaaacttg tggacttttg aaacctgttg actaaacagt aattaattta
     8941 tatttgtgaa aaatgccact gtcctggtga tttctgatgt aaataatgtt gtttatatag
     9001 tatgtattaa attttcctac attgtaaaac tgctgtactt ttttgattct tgtatattaa
     9061 aaagtgttac t
//
