LOCUS       NM_009891               2810 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus choline O-acetyltransferase (Chat), mRNA.
ACCESSION   NM_009891
VERSION     NM_009891.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Sitko,A.A., Frank,M.M., Romero,G.E., Hunt,M. and Goodrich,L.V.
  TITLE     Lateral olivocochlear neurons modulate cochlear responses to noise
            exposure
  JOURNAL   Proc Natl Acad Sci U S A 122 (4), e2404558122 (2025)
   PUBMED   39854232
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Pagiazitis,J.G., Delestree,N., Sowoidnich,L., Sivakumar,N.,
            Simon,C.M., Chatzisotiriou,A., Albani,M. and Mentis,G.Z.
  TITLE     Catecholaminergic dysfunction drives postural and locomotor
            deficits in a mouse model of spinal muscular atrophy
  JOURNAL   Cell Rep 44 (1), 115147 (2025)
   PUBMED   39752251
REFERENCE   3  (bases 1 to 2810)
  AUTHORS   Fritz,M., Rosa,P.B., Wilhelms,D., Jaarola,M., Ruud,J., Engblom,D.
            and Klawonn,A.M.
  TITLE     Nicotinic alpha7 receptors on cholinergic neurons in the striatum
            mediate cocaine-reinforcement, but not food reward
  JOURNAL   Front Mol Neurosci 17, 1418686 (2025)
   PUBMED   39906479
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2810)
  AUTHORS   Harima,Y., Tsurutani,M., Yamada,S., Uchida,S., Inada,K.,
            Hagihara,M., Irie,S., Shigeta,M., Abe,T., Inoue,Y.U., Inoue,T. and
            Miyamichi,K.
  TITLE     Parallel labeled-line organization of sympathetic outflow for
            selective organ regulation in mice
  JOURNAL   Nat Commun 15 (1), 10478 (2024)
   PUBMED   39658565
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2810)
  AUTHORS   Mueller,J.L., Leavitt,A.R., Rahman,A.A., Han,C.Y., Ott,L.C.,
            Mahdavian,N.S., Carbone,S.E., King,S.K., Burns,A.J., Poole,D.P.,
            Hotta,R., Goldstein,A.M. and Stavely,R.
  TITLE     Highly neurogenic glia from human and mouse myenteric ganglia
            generate functional neurons following culture and transplantation
            into the gut
  JOURNAL   Cell Rep 43 (11), 114919 (2024)
   PUBMED   39471175
REFERENCE   6  (bases 1 to 2810)
  AUTHORS   Motwani,M., White,R.A., Guo,N., Dowler,L.L., Tauber,A.I. and
            Sastry,K.N.
  TITLE     Mouse surfactant protein-D. cDNA cloning, characterization, and
            gene localization to chromosome 14
  JOURNAL   J Immunol 155 (12), 5671-5677 (1995)
   PUBMED   7499852
REFERENCE   7  (bases 1 to 2810)
  AUTHORS   Schneider-Maunoury,S., Topilko,P., Seitandou,T., Levi,G.,
            Cohen-Tannoudji,M., Pournin,S., Babinet,C. and Charnay,P.
  TITLE     Disruption of Krox-20 results in alteration of rhombomeres 3 and 5
            in the developing hindbrain
  JOURNAL   Cell 75 (6), 1199-1214 (1993)
   PUBMED   7903221
REFERENCE   8  (bases 1 to 2810)
  AUTHORS   Misawa,H., Ishii,K. and Deguchi,T.
  TITLE     Gene expression of mouse choline acetyltransferase. Alternative
            splicing and identification of a highly active promoter region
  JOURNAL   J Biol Chem 267 (28), 20392-20399 (1992)
   PUBMED   1400357
REFERENCE   9  (bases 1 to 2810)
  AUTHORS   Whitlon,D.S. and Rutishauser,U.S.
  TITLE     NCAM in the organ of Corti of the developing mouse
  JOURNAL   J Neurocytol 19 (6), 970-977 (1990)
   PUBMED   2292721
REFERENCE   10 (bases 1 to 2810)
  AUTHORS   Ishii,K., Oda,Y., Ichikawa,T. and Deguchi,T.
  TITLE     Complementary DNAs for choline acetyltransferase from spinal cords
            of rat and mouse: nucleotide sequences, expression in mammalian
            cells, and in situ hybridization
  JOURNAL   Brain Res Mol Brain Res 7 (2), 151-159 (1990)
   PUBMED   2160042
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK046406.1.
            
            On Apr 5, 2007 this sequence version replaced NM_009891.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK046406.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2810              AK046406.1         2-2811
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 19.4 cM"
     gene            1..2810
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /note="choline O-acetyltransferase"
                     /db_xref="GeneID:12647"
                     /db_xref="MGI:MGI:88392"
     exon            1..103
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            104..292
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            293..396
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     CDS             331..2268
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /EC_number="2.3.1.6"
                     /note="choline acetylase; choline acetyltransferase"
                     /codon_start=1
                     /product="choline O-acetyltransferase"
                     /protein_id="NP_034021.1"
                     /db_xref="CCDS:CCDS26920.1"
                     /db_xref="GeneID:12647"
                     /db_xref="MGI:MGI:88392"
                     /translation="MPILEKVPPKMPVQASSCEEVLDLPKLPVPPLQQTLATYLQCMQ
                     HLVPEEQFRKSQAIVKRFGAPGGLGETLQEKLLERQEKTANWVSEYWLNDMYLNNRLA
                     LPVNSSPAVIFARQHFQDTNDQLRFAASLISGVLSYKALLDSQSIPTDWAKGQLSGQP
                     LCMKQYYRLFSSYRLPGHTQDTLVAQKSSIMPEPEHVIVACCNQFFVLDVVINFRRLS
                     EGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAKARTVLLKDSTNRDSLDMIER
                     CICLVCLDGPGTGDLSDTHRALQLLHGGGCSLNGANRWYDKSLQFVVGRDGTCGVVCE
                     HSPFDGIVLVQCTEHLLKHMMTGNKKLVRADSVSELPAPRRLRWKCSPETQGHLASSA
                     EKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDGFIQVALQLAYYRLYQRLVPTYESA
                     SIRRFQEGRVDNIRSATPEALAFVQAMTDHKAAVLASEKLQLLQRAIQAQTEYTVMAI
                     TGMAIDNHLLALRELARDLCKEPPEMFMDETYLMSNRFILSTSQVPTTMEMFCCYGPV
                     VPNGYGACYNPQPEAITFCISSFHGCKETSSVEFAEAVGASLVDMRDLCSSRQPAEGK
                     PPTAKERARGPTKPSNLDYSH"
     exon            397..588
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            589..707
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            708..761
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            762..942
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1120
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1290
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1391
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1520
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1643
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            1644..1785
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..1848
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..1986
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
     exon            1987..2810
                     /gene="Chat"
                     /gene_synonym="B230380D24Rik; CHOACTase"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgtgcagcc ctcccggaag gaaggtgagc cttcctaagc ctctactgac agcaaagctg
       61 cagaggccct gctgcgtgag acccagaagc ttccacgcca ctttcagtca gtcggggcgg
      121 ctgctgggat ctggcaactt cgtcggaggc tctgctacag aacctaggtg gcgggcccaa
      181 cctctggtac tgctgccacc ccctccctgg cccttctggc tcacgcagcc gcctccagcc
      241 ctgcttggtg tggaacagtg ccggttcggt gcgtaacagc ccaggagagc aggtcggcag
      301 ctctgctact ctggattaag aatcgctagg atgcctatcc tggaaaaggt ccccccaaag
      361 atgcctgtac aagcttctag ctgtgaggag gtgctggact tacctaagtt gccagtgccc
      421 ccactgcagc aaaccctggc cacctacctt cagtgcatgc aacacctggt acctgaagag
      481 cagttcagga agagccaggc cattgtgaag cggtttgggg cccctggtgg cctgggtgag
      541 accctgcagg aaaagctctt ggagagacag gagaagacag ccaattgggt ctctgaatac
      601 tggctgaatg acatgtatct aaacaaccgc ctggccctgc cagtcaactc tagccctgct
      661 gtgatctttg ctcggcagca cttccaagac accaatgacc agctaaggtt tgcagccagc
      721 ctcatctctg gtgtgcttag ctacaaggct ctgctggaca gccaatccat tcccactgac
      781 tgggccaagg ggcagctctc agggcagcct ctctgtatga agcagtacta cagactcttc
      841 tcatcatacc ggcttcctgg ccatacccag gacacactgg tggcccagaa gagcagtatc
      901 atgcctgagc ccgagcatgt catcgtggcc tgctgcaacc agttctttgt cttggatgtt
      961 gtcattaatt tccgccgtct cagtgagggt gatctgttca ctcagttgag aaagatagtc
     1021 aaaatggcgt ccaacgagga tgaacgcctg cctccaatcg gcctgctgac gtcagacggg
     1081 aggagcgagt gggccaaggc caggacggtc ctcttaaaag actccaccaa ccgggactcc
     1141 ctggacatga tcgagcgctg catctgcctg gtatgcctgg atggtccagg cactggagac
     1201 ctcagtgaca cacacagggc cctccagctc cttcatggtg gaggctgcag cttgaatgga
     1261 gcgaatcgtt ggtatgacaa gtccctgcag tttgtggtgg gccgagatgg cacctgcggt
     1321 gtggtgtgtg agcactcccc ttttgatggc atcgtcctgg tgcagtgcac ggagcacctg
     1381 ctgaaacata tgatgacagg caacaagaag ctcgtccgag ctgactcagt gagtgaactc
     1441 cctgctccca gaaggctgag gtggaaatgt tccccagaaa ctcaaggcca tctcgcctcc
     1501 tcggcagaga aacttcaaag aatcgtaaag aatctggatt tcattgttta taagtttgac
     1561 aactatggga aaacatttat taagaagcag aaatgcagtc ctgatggctt catccaggtg
     1621 gccctccagc tggcttacta caggctttac cagaggctgg tgcccaccta tgagagtgca
     1681 tccatccgcc gcttccagga aggtcgggtg gacaacatca gatcggccac tcctgaggct
     1741 ctggcttttg tgcaagccat gactgaccac aaggctgccg tgctggcttc tgagaaactg
     1801 cagctgctgc agagggccat ccaggcccaa actgagtaca cagtcatggc cataaccggc
     1861 atggccattg acaaccatct tctggcactg agggagctgg cccgagacct gtgcaaagag
     1921 ccacctgaga tgttcatgga tgaaacatac ctgatgagca accggtttat tctctccacc
     1981 agccaggtgc ccacgaccat ggagatgttc tgctgttatg gccctgtggt acccaatggg
     2041 tatggagcct gttataaccc ccagcctgag gccatcacct tctgcatctc cagctttcac
     2101 ggctgcaaag agacctcatc tgtggagttt gcagaagcgg tgggagcgag ccttgttgac
     2161 atgagagacc tctgtagttc gaggcagcct gctgaaggca agccaccaac agcaaaggaa
     2221 agagctagag gcccaaccaa gccaagcaat cttgactact cccactagcc aatgtcctac
     2281 aggagtcagc ccctactaac cctgctccca tcccccaccc cagcttgttg ctgctcccct
     2341 atccttgggg gctcacatga agctggcatg ttaagagaga gagagcccct cattatctat
     2401 ctaagtggcc tgtggccttt acaactggaa atgagaccca gcctggcttg gaagcagcct
     2461 gggtgggctg ggagctccct ctgaggtctt tagaaactta acctttctgc ttctttccca
     2521 gcaacaccca gtggtgcaca tggtagctct gccagtggaa gaatcgtcat ctcatcacat
     2581 gctatgggcc caaataagtc ataaaggcag aggctagctc cctccacaca ctcacatgca
     2641 caaagacagc ccaagttcat ttagaataca gtggcccaca tccacaaaga catttcttcc
     2701 tttatcttcc atagcacagt cttccttgag ttcagattat ctcatccagc tattcagaaa
     2761 gaaagaaaga aagaaagaaa gaaagaaaga aagaaagaaa gaaaggaagg
//
