LOCUS       NM_026527               1330 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus ChaC, cation transport regulator 2 (Chac2), transcript
            variant 1, mRNA.
ACCESSION   NM_026527
VERSION     NM_026527.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Kaur,A., Gautam,R., Srivastava,R., Chandel,A., Kumar,A.,
            Karthikeyan,S. and Bachhawat,A.K.
  TITLE     ChaC2, an Enzyme for Slow Turnover of Cytosolic Glutathione
  JOURNAL   J Biol Chem 292 (2), 638-651 (2017)
   PUBMED   27913623
  REMARK    GeneRIF: Human and mouse ChaC2 proteins purified in vitro show
            10-20-fold lower catalytic efficiency than ChaC1, although they
            showed comparable Km values
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY333527.1, AK078993.1 and AL662891.9.
            
            On Mar 15, 2014 this sequence version replaced NM_026527.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC025100.1, AK010914.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                BY333527.1         1-15
            16-1290             AK078993.1         2-1276
            1291-1330           AL662891.9         215115-215154       c
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 18.2 cM"
     gene            1..1330
                     /gene="Chac2"
                     /note="ChaC, cation transport regulator 2"
                     /db_xref="GeneID:68044"
                     /db_xref="MGI:MGI:1915294"
     exon            1..254
                     /gene="Chac2"
                     /inference="alignment:Splign:2.1.0"
     CDS             120..656
                     /gene="Chac2"
                     /EC_number="4.3.2.7"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cation transport regulator-like protein 2; ChaC, cation
                     transport regulator homolog 2; gamma-GCG 2; putative
                     glutathione-specific gamma-glutamylcyclotransferase 2"
                     /codon_start=1
                     /product="putative glutathione-specific
                     gamma-glutamylcyclotransferase 2 isoform 1"
                     /protein_id="NP_080803.1"
                     /db_xref="CCDS:CCDS24510.1"
                     /db_xref="GeneID:68044"
                     /db_xref="MGI:MGI:1915294"
                     /translation="MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPG
                     KPGRVVTLVEDPGGSVWGVAYKLPVGKEEEVKTYLDFREKGGYRTTTVIFYPKDSTTK
                     PFSVLLYIGTCDNPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLVPEDA
                     DEHLFSLEKLVKERLEGK"
     exon            255..290
                     /gene="Chac2"
                     /inference="alignment:Splign:2.1.0"
     exon            291..1330
                     /gene="Chac2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1061..1066
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chac2"
                     /note="hexamer: ATTAAA"
     regulatory      1065..1070
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chac2"
                     /note="hexamer: AATGAA"
     polyA_site      1081
                     /gene="Chac2"
                     /note="major polyA site"
     polyA_site      1089
                     /gene="Chac2"
                     /note="major polyA site"
     regulatory      1205..1210
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chac2"
                     /note="hexamer: TATAAA"
     polyA_site      1228
                     /gene="Chac2"
     regulatory      1293..1298
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chac2"
                     /note="hexamer: AATAAA"
     polyA_site      1327
                     /gene="Chac2"
ORIGIN      
        1 gtgtgctttg cgccgcacat caggcgaagc cagagcgcct ggcactgccc cagcagccgg
       61 ttactcccct cggtagcagt ctcgttctga agtccgagtg ccggtcaggg tccagaaaga
      121 tgtgggtgtt tggttatggg tccctcatct ggaaggtgga cttcccctac caggacaagc
      181 tggttggtta catcacaaac tacagtcggc gcttctggca aggtagcacg gaccaccgcg
      241 gggtcccagg caagcctgga agagttgtga cccttgttga agatcctggg ggcagtgtat
      301 ggggtgttgc ttacaaacta ccagtaggaa aagaagagga agtaaaaaca taccttgact
      361 tcagagagaa aggaggctac agaactacga cagtcatttt ttatccaaaa gattccacaa
      421 caaaaccatt cagtgttttg ctctacattg gaacatgtga taatcctaac tatcttggcc
      481 ctgcacctct ggaagatatt gctgaacaaa tttttaatgc agctggtcca agtggaagaa
      541 atacagaata tctttttgaa cttgcagatt ctgttaggaa acttgtacca gaagatgcag
      601 acgagcatct cttctctttg gaaaaattag taaaggaacg cttagaagga aaatagaaac
      661 tgatttgcat acagagtcac caacgcaccc aaagaggcaa gagttttagt cttcagaaaa
      721 taacatcagt acagaacatc agtatgtttt agacattctt ccttgaagat cttactattt
      781 aatattgtaa ttggaatatc atctagattt acgatgtctt tgcaagtgtc ctgaaacata
      841 tccaacagat tctcacaatg ggaaagagtt cacgttgtaa aagtgatttc ctagttatac
      901 tacatttcaa catttgttca ttagaaatgc aggtctcaat taacttcctg tttctattag
      961 aataaccatg gtttgttgta cgtaagttta aaataatgtt attagaaata tagaagaggt
     1021 aaggttacag cactgattgt taatatcctt gttatttgga attaaatgaa cctaaaattt
     1081 agaccaaaag aaattgtact tttcttttaa tgtaaattat ccctttggga ttataatatg
     1141 ctttctattc ctttgggtta agaaggtatt ttataaccaa gaaaactaca tagagctatc
     1201 catatataaa accactatga taatcatatt tactttgtat ttatttcata atgaaacttt
     1261 aaaataaaag ataggttttc tggtggaaac ataataaaaa ttgtgtttaa aacagatttt
     1321 acaattataa
//
