LOCUS       NM_009890               1366 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus cholesterol 25-hydroxylase (Ch25h), mRNA.
ACCESSION   NM_009890
VERSION     NM_009890.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Chen,K.Y., De Giovanni,M., Xu,Y., An,J., Kirthivasan,N., Lu,E.,
            Jiang,K., Brooks,S., Ranucci,S., Yang,J., Kanameishi,S.,
            Kabashima,K., Brulois,K., Bscheider,M., Butcher,E.C. and
            Cyster,J.G.
  TITLE     Inflammation switches the chemoattractant requirements for naive
            lymphocyte entry into lymph nodes
  JOURNAL   Cell 188 (4), 1019-1035 (2025)
   PUBMED   39708807
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Diercks,A.H., Podolskaia,I.S., Murray,T.A., Jahn,A.N., Mai,D.,
            Liu,D., Amon,L.M., Nakagawa,Y., Shimano,H., Aderem,A. and Gold,E.S.
  TITLE     Oxysterol binding protein regulates the resolution of TLR-induced
            cytokine production in macrophages
  JOURNAL   Proc Natl Acad Sci U S A 121 (33), e2406492121 (2024)
   PUBMED   39361877
REFERENCE   3  (bases 1 to 1366)
  AUTHORS   Zhang,L., Fang,Z., Zhu,Q., Yang,S., Fu,J., Sun,Z., Lu,G., Wei,C.,
            Zhang,Z., Lee,K., Zhong,Y., Liu,R. and He,J.C.
  TITLE     Cholesterol 25-Hydroxylase Protects Against Diabetic Kidney Disease
            by Regulating ADP Ribosylation Factor 4
  JOURNAL   Adv Sci (Weinh) 11 (29), e2309642 (2024)
   PUBMED   38816950
  REMARK    GeneRIF: Cholesterol 25-Hydroxylase Protects Against Diabetic
            Kidney Disease by Regulating ADP Ribosylation Factor 4.
REFERENCE   4  (bases 1 to 1366)
  AUTHORS   Zhang,J., Zhou,H., Lei,F., Jiang,K., Liao,Y., Huang,F. and Chen,M.
  TITLE     Cholesterol 25-hydroxylase prevents type 2 diabetes mellitus
            induced cardiomyopathy by alleviating cardiac lipotoxicity
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (5), 167158 (2024)
   PUBMED   38588780
  REMARK    GeneRIF: Cholesterol 25-hydroxylase prevents type 2 diabetes
            mellitus induced cardiomyopathy by alleviating cardiac
            lipotoxicity.
REFERENCE   5  (bases 1 to 1366)
  AUTHORS   Toral-Rios,D., Long,J.M., Ulrich,J.D., Yu,J., Strickland,M.R.,
            Han,X., Holtzman,D.M., Cashikar,A.G. and Paul,S.M.
  TITLE     Cholesterol 25-hydroxylase mediates neuroinflammation and
            neurodegeneration in a mouse model of tauopathy
  JOURNAL   J Exp Med 221 (4) (2024)
   PUBMED   38442267
  REMARK    GeneRIF: Cholesterol 25-hydroxylase mediates neuroinflammation and
            neurodegeneration in a mouse model of tauopathy.
REFERENCE   6  (bases 1 to 1366)
  AUTHORS   Zou,T., Garifulin,O., Berland,R. and Boyartchuk,V.L.
  TITLE     Listeria monocytogenes infection induces prosurvival metabolic
            signaling in macrophages
  JOURNAL   Infect Immun 79 (4), 1526-1535 (2011)
   PUBMED   21263022
  REMARK    GeneRIF: We found that expression of Ch25h is upregulated following
            L. monocytogenes infection
REFERENCE   7  (bases 1 to 1366)
  AUTHORS   Diczfalusy,U., Olofsson,K.E., Carlsson,A.M., Gong,M.,
            Golenbock,D.T., Rooyackers,O., Flaring,U. and Bjorkbacka,H.
  TITLE     Marked upregulation of cholesterol 25-hydroxylase expression by
            lipopolysaccharide
  JOURNAL   J Lipid Res 50 (11), 2258-2264 (2009)
   PUBMED   19502589
  REMARK    GeneRIF: Data show that The increased Ch25h expression was
            dependent on Toll-like receptor 4 signaling.
REFERENCE   8  (bases 1 to 1366)
  AUTHORS   Bauman,D.R., Bitmansour,A.D., McDonald,J.G., Thompson,B.M.,
            Liang,G. and Russell,D.W.
  TITLE     25-Hydroxycholesterol secreted by macrophages in response to
            Toll-like receptor activation suppresses immunoglobulin A
            production
  JOURNAL   Proc Natl Acad Sci U S A 106 (39), 16764-16769 (2009)
   PUBMED   19805370
  REMARK    GeneRIF: deletion of the mouse cholesterol 25-hydroxylase gene
            caused an increase in serum IgA.
REFERENCE   9  (bases 1 to 1366)
  AUTHORS   Russell,D.W.
  TITLE     The enzymes, regulation, and genetics of bile acid synthesis
  JOURNAL   Annu Rev Biochem 72, 137-174 (2003)
   PUBMED   12543708
  REMARK    Review article
REFERENCE   10 (bases 1 to 1366)
  AUTHORS   Lund,E.G., Kerr,T.A., Sakai,J., Li,W.P. and Russell,D.W.
  TITLE     cDNA cloning of mouse and human cholesterol 25-hydroxylases,
            polytopic membrane proteins that synthesize a potent oxysterol
            regulator of lipid metabolism
  JOURNAL   J Biol Chem 273 (51), 34316-34327 (1998)
   PUBMED   9852097
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102249.16.
            
            On Jan 22, 2019 this sequence version replaced NM_009890.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: AF059213.1, AK160657.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1366              AC102249.16        103004-104369       c
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 29.69 cM"
     gene            1..1366
                     /gene="Ch25h"
                     /gene_synonym="m25OH"
                     /note="cholesterol 25-hydroxylase"
                     /db_xref="GeneID:12642"
                     /db_xref="MGI:MGI:1333869"
     exon            1..1366
                     /gene="Ch25h"
                     /gene_synonym="m25OH"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..919
                     /gene="Ch25h"
                     /gene_synonym="m25OH"
                     /EC_number="1.14.99.38"
                     /note="cholesterol 25-monooxygenase"
                     /codon_start=1
                     /product="cholesterol 25-hydroxylase"
                     /protein_id="NP_034020.1"
                     /db_xref="CCDS:CCDS29759.1"
                     /db_xref="GeneID:12642"
                     /db_xref="MGI:MGI:1333869"
                     /translation="MGCYNGSELQDLGCSSQLLLQPLWDTIRTREAFTRSPIFPVTFS
                     IITYVGFCLPFVVLDVLYPWVPILRRYKIHPDFSPSVKQLLPCLGLTLYQHLVFVFPV
                     TLLHWVRSPALLPQEAPELVQLLSHVLICLLLFDTEIFAWHLLHHKVPWLYRTFHKVH
                     HQNSSSFALATQYMSFWELLSLTFFDVLNVAVLRCHPLTIFTFHVINIWLSVEDHSGY
                     DFPWSTHRLVPFGWYGGVAHHDMHHSQFNCNFAPYFTHWDKMLGTLRSAPLPESLCAC
                     GERCVNSRERCAVHLIQKKKQT"
     regulatory      1345..1350
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ch25h"
                     /gene_synonym="m25OH"
                     /note="hexamer: AATAAA"
     polyA_site      1366
                     /gene="Ch25h"
                     /gene_synonym="m25OH"
                     /note="major polyA site"
ORIGIN      
        1 aattgtggga catagtctca gcatgggctg ctacaacggt tcggagctcc aagacctggg
       61 ctgttccagc cagctgctcc tgcagcccct ctgggacacc ataaggacaa gggaggcgtt
      121 cacgcgctca cccatcttcc cagtcacctt ttctatcatc acttacgtgg gcttctgcct
      181 accgttcgtg gtgctggacg tcctgtatcc ctgggtcccc atcctgcgac gctacaagat
      241 ccacccggac ttctcgcctt ccgtaaagca gcttctgcct tgcctggggc tgacactcta
      301 ccagcacctg gtgttcgtgt tcccggtgac gctgctgcac tgggtgcgca gcccggcgct
      361 cctcccccag gaggcccctg agctcgtcca gctcctaagt cacgtcctga tctgcctgct
      421 gctcttcgac accgagatct tcgcgtggca cctgctgcac cataaggtgc cctggctgta
      481 ccgcaccttc cacaaggtgc atcaccagaa ctcgtcctcc ttcgcgctgg cgacccaata
      541 catgagcttc tgggagctgc tttcgctgac cttcttcgac gtgctgaacg tcgcggtgct
      601 tcggtgtcac ccactcacca tctttacctt tcacgtgatt aacatctggc tgtcggtgga
      661 ggaccactcg ggctatgact tcccgtggtc cactcacaga cttgtgccct ttggctggta
      721 cgggggcgtg gctcaccacg acatgcatca ctctcagttt aactgcaatt ttgctcctta
      781 cttcacacac tgggacaaaa tgctgggcac tctgcggtcc gcgcccctgc cagagagcct
      841 ttgcgcctgc ggtgagcgct gcgtgaactc cagagagcga tgcgctgtac acttgatcca
      901 gaagaagaaa cagacttgag ttactttatt agaagtttgt aacttttaaa gtgatgcaca
      961 ctgctataaa atctaatgtt gtttctgcag cctgacaaag taatttatat aatgtttcta
     1021 tgtgaattta attgtggtct tggtgttaaa tttcaactta tcccagtgtc attgactcca
     1081 ggacacaagg tagacattca gcgtggtgta tttgaatcat tatgatctct gaaatgattt
     1141 gtttctagaa ccaagacttt cctatgtatg aaatcgcact ggaactgggc tctaacctca
     1201 catgttgaga gaagacctga tttctgactc tttaaataat caaatttgtt cctgctgggc
     1261 agtgaccaag gttatagtat attttttcta cttttgaatg aaatgatata tttatattga
     1321 aaaaagtttt atttgtattt taaaaataaa aagaacatga actaaa
//
