LOCUS       NM_029698               3621 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus cilia and flagella associated protein 70 (Cfap70),
            transcript variant 3, mRNA.
ACCESSION   NM_029698 XM_127606 XM_980494
VERSION     NM_029698.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Jin,H.J., Wang,J.L., Geng,X.Y., Wang,C.Y., Wang,B.B. and Chen,S.R.
  TITLE     CFAP70 is a solid and valuable target for the genetic diagnosis of
            oligo-astheno-teratozoospermia in infertile men
  JOURNAL   EBioMedicine 93, 104675 (2023)
   PUBMED   37352829
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Shamoto,N., Narita,K., Kubo,T., Oda,T. and Takeda,S.
  TITLE     CFAP70 Is a Novel Axoneme-Binding Protein That Localizes at the
            Base of the Outer Dynein Arm and Regulates Ciliary Motility
  JOURNAL   Cells 7 (9), 124 (2018)
   PUBMED   30158508
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3621)
  AUTHORS   McClintock,T.S., Glasser,C.E., Bose,S.C. and Bergman,D.A.
  TITLE     Tissue expression patterns identify mouse cilia genes
  JOURNAL   Physiol Genomics 32 (2), 198-206 (2008)
   PUBMED   17971504
  REMARK    GeneRIF: Ttc18 is most abundantly expressed in tissues rich in
            highly ciliated cells, such as olfactory sensory neurons, and is
            predicted to be important to cilia.
REFERENCE   4  (bases 1 to 3621)
  AUTHORS   Lin,Q., Donahue,S.L., Moore-Jarrett,T., Cao,S., Osipovich,A.B. and
            Ruley,H.E.
  TITLE     Mutagenesis of diploid mammalian genes by gene entrapment
  JOURNAL   Nucleic Acids Res 34 (20), e139 (2006)
   PUBMED   17062627
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121801.2.
            
            On or before Jul 26, 2009 this sequence version replaced
            XM_980494.1, XM_127606.7.
            
            Transcript Variant: This variant (3) differs in the 5' UTR, lacks
            one in-frame exon and contains an alternate in-frame exon in the
            central coding region, compared to variant 1. The encoded isoform
            (3) is longer, compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029536.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849379 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AC121801.2         37958-37981
            25-127              AC121801.2         41526-41628
            128-314             AC121801.2         42562-42748
            315-413             AC121801.2         46224-46322
            414-493             AC121801.2         46501-46580
            494-673             AC121801.2         49380-49559
            674-856             AC121801.2         50336-50518
            857-951             AC121801.2         53366-53460
            952-1051            AC121801.2         58160-58259
            1052-1186           AC121801.2         60654-60788
            1187-1354           AC121801.2         64654-64821
            1355-1435           AC121801.2         64963-65043
            1436-1527           AC121801.2         65719-65810
            1528-1588           AC121801.2         69048-69108
            1589-1783           AC121801.2         69364-69558
            1784-1915           AC121801.2         73555-73686
            1916-2041           AC121801.2         77711-77836
            2042-2175           AC121801.2         80749-80882
            2176-2291           AC121801.2         81001-81116
            2292-2504           AC121801.2         81502-81714
            2505-2573           AC121801.2         85527-85595
            2574-2732           AC121801.2         85927-86085
            2733-2854           AC121801.2         86993-87114
            2855-3007           AC121801.2         88941-89093
            3008-3169           AC121801.2         89665-89826
            3170-3259           AC121801.2         92911-93000
            3260-3361           AC121801.2         95168-95269
            3362-3409           AC121801.2         95503-95550
            3410-3621           AC121801.2         95782-95993
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 11.53 cM"
     gene            1..3621
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /note="cilia and flagella associated protein 70"
                     /db_xref="GeneID:76670"
                     /db_xref="MGI:MGI:1923920"
     exon            1..24
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            25..127
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..3490
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /note="isoform 3 is encoded by transcript variant 3;
                     tetratricopeptide repeat domain 18; TPR repeat protein 18;
                     tetratricopeptide repeat protein 18"
                     /codon_start=1
                     /product="cilia- and flagella-associated protein 70
                     isoform 3"
                     /protein_id="NP_083974.1"
                     /db_xref="CCDS:CCDS49413.1"
                     /db_xref="GeneID:76670"
                     /db_xref="MGI:MGI:1923920"
                     /translation="MDQTSSTTKIVHITVTNGYDLKGFKGDTPVTFVRAEFNQTVLGD
                     SSKVTVSPEGTAKYNFTSNIDLSPDGGGALDDLAHKPLFLTVTEVLPKEKKQKDEKTL
                     ILGQAVVDLLPLLEGEESFETTVPLHPVPGSPLETPLPGSKQCSLDVKVFVAEPLLTP
                     AQVSASNLLKVTLEAAYSVPESFIPVGPQQNYMVGLQVPSVGEKDYTMIFKNGNLKLG
                     GEKEPVPRPKKWPIANILAPGASNIPDEFIVGGPYEEEEGELNHPEDREFRNQAECTK
                     KRIVWDLESRCFLHPFAVASFQKRIADCRLWPVEITRVPLVVMPKAKPGKLEKIDDEN
                     QLSFHGVAYINMVPLLYPGVKKIRGAFHVYPYLDGTVFEKTKCLFSLFRDTGHHLVQN
                     NKAGGLNSPLSKPLSKNLKEEKPGKDKDTEGRPRLGELQAPSIKSQSSDTPLESEAPL
                     SHNLEGQQYIEAGTYIVLEIQLEKALVPKRMPEELARRVKEMIPPRPPLTRRTGGAQK
                     AVSDYHTQIKSISRAILNEYYRMFGKQGPKLESDIDNETMEERKCQLNYELNCSGKYF
                     AFKEQLKHAVVKIVREKYLKTTAFESQEELQTFISELYVFLVDQMHVALNQAVPDDVP
                     SSTSTIQTSSEQLRLFAFEAEVNEKFEIAAMYYEERLVREPQNLENWLDYGAFCLLTE
                     DNIKAQECFRKALSLNESHVDSLLLCGVLAILLENYEQAEIFFEDATCLEPTNVIAWT
                     LLGLFYEIQNNDIRMEMAFHEAFKQLQARTLQTKLKSTVTIENMEEGVKVEPSFGPWG
                     VVQESTTAIKTEGLSGMRPQSSHQLSPHTNMELHPQPQGPNTALSSLDEFLEESPKAQ
                     SESQEPMATGQPLEPSLVQRSSNALLKELTSKKDISKCQDSSAFSPPTQHVIAQPPVT
                     IFMETIRFLMKVNAVQFVHRVLAHELLCPQGGPSCEYYLVLAQTHLLKKDFAKTEEYL
                     QQAAQMDYLNPNVWGVKGHLYFLSGNHAEAKECYERTISFVVDASEMHFIFLRLGHIY
                     LEEKEYENAKRTYMHACKRSPSCLTWLGLGIACYRLEELTEAEDALSEANALNNCNAE
                     VWAYLALVCLKVGRQLEAEQAYKYTIKLKLKDQALLEEIHTVQEMVGFGNPSF"
     exon            128..314
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            315..413
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            414..493
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            494..673
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            674..856
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            857..951
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1051
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1186
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1354
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1435
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1527
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1588
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1783
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..1915
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2041
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2175
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            2176..2291
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2504
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            2505..2573
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            2574..2732
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            2733..2854
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            2855..3007
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            3008..3169
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            3170..3259
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            3260..3361
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            3362..3409
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     exon            3410..3621
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3589..3594
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /note="hexamer: ATTAAA"
     polyA_site      3611
                     /gene="Cfap70"
                     /gene_synonym="Ttc18"
                     /note="major polyA site"
ORIGIN      
        1 acagaaaacc agcgtctgcc tcagaggcca aagaagacca gagagacttt gaccaagtgc
       61 agacatggac cagacgtcaa gcacaacgaa aattgtgcac attactgtga caaatggata
      121 tgatttgaaa ggcttcaaag gagacacccc agttactttt gtccgtgcag aattcaacca
      181 gacagttctt ggagactctt caaaagtcac tgtctctccc gaaggaactg caaagtacaa
      241 ctttactagc aacattgacc tcagcccaga tggaggcggt gccttagatg acctcgccca
      301 caagccttta ttcttaacgg tgactgaagt tttgccaaag gaaaagaaac agaaagatga
      361 aaagacctta attcttggcc aagctgtggt ggatcttctt cctttactag aaggagagga
      421 gtcatttgaa acaacggtcc cattacaccc tgtgcctggg tcacccttgg aaactccttt
      481 gccaggctct aagcaatgca gtcttgatgt gaaggtattt gtggccgagc ccttattgac
      541 cccagcccag gtctcagcga gcaatctact gaaggtcaca ctggaggccg cctactctgt
      601 gcccgagtcc ttcataccag tagggcctca gcagaactac atggtcggtc tgcaggtccc
      661 atcagtcgga gagaaggact acactatgat attcaagaat ggaaatctga agcttggagg
      721 ggaaaaagaa cctgttcccc ggcctaaaaa atggcccatt gctaacattc tagctccagg
      781 agctagtaac attcctgacg aattcattgt ggggggtccc tatgaggaag aagaaggaga
      841 actcaaccac cctgaggaca gggaatttag gaaccaagcc gaatgcacga agaagaggat
      901 tgtctgggat ttggaaagcc gctgcttcct ccatcctttt gccgttgcaa gttttcagaa
      961 gcgaattgct gattgccgac tctggcctgt ggagatcaca agagtgcctt tggtcgttat
     1021 gccaaaagcc aaaccaggaa aacttgagaa gatcgatgat gaaaaccagc tttcctttca
     1081 tggtgtggct tacatcaaca tggtcccact cctgtatcca ggagtgaaga agataagagg
     1141 ggcttttcat gtgtacccct acctggatgg gacagtcttc gaaaagacca agtgcttgtt
     1201 cagcttattc agggacactg ggcaccattt ggtccaaaat aataaagcag ggggacttaa
     1261 ctctccactg tccaaacctc tctccaagaa tctgaaagaa gagaaaccag ggaaagacaa
     1321 ggacacagaa ggacggcctc ggcttggaga gctgcaagcg cctagtataa aatcacagag
     1381 ctcagacacc cctttggaaa gtgaagcccc cctgagccac aaccttgaag gacagcaata
     1441 catagaagcg ggaacataca ttgtcctgga gatccagctg gaaaaagccc tggttccaaa
     1501 gcggatgcca gaggaactgg ccagaagggt caaggaaatg atacccccga ggccacctct
     1561 cactcgccgg acagggggcg ctcagaaggc tgtgagtgac taccacacgc agatcaagag
     1621 catttctaga gccatactca atgaatacta ccgcatgttt gggaagcaag ggcctaaact
     1681 ggagagtgac atagacaacg agaccatgga ggagcggaag tgtcagctca actatgagct
     1741 caattgctct ggaaagtact ttgctttcaa agaacagctc aagcatgctg tcgtaaagat
     1801 tgtcagagag aaatacttga agacaacagc attcgaaagc caggaggaac tgcagacatt
     1861 tatcagtgag ctgtatgtgt tcttggtaga tcaaatgcat gtggccctga accaggccgt
     1921 cccggatgat gtcccaagca gtacctcaac cattcagaca agcagcgagc agctccgact
     1981 ctttgcgttt gaggcagaag tcaatgagaa gtttgaaata gcagcaatgt attatgaaga
     2041 gaggttggtt cgggagcccc agaacctgga aaactggctg gattacggcg ctttctgtct
     2101 cctcactgaa gacaacatca aagcgcagga atgtttccgg aaagcccttt ctctgaatga
     2161 gagtcacgtt gacagcttgt tgctgtgtgg tgtcctggct atcttgttgg agaactatga
     2221 gcaagcagaa atcttctttg aggatgctac atgcttggaa cccaccaatg ttattgcctg
     2281 gactttactc ggattgttct atgaaattca gaataatgat attcgaatgg aaatggcatt
     2341 tcatgaggcc ttcaaacagc ttcaggcgcg aaccctccaa acaaagctaa aaagcactgt
     2401 gaccatagag aacatggagg aaggagtgaa agtggagccc agctttggcc cttggggagt
     2461 cgtacaggag tccaccacag ccatcaaaac ggaaggccta tcagggatga gaccccagag
     2521 ctcacaccag ctgagtcctc acaccaacat ggaactgcat cctcagcccc aaggaccaaa
     2581 cactgctttg tccagtctag acgagttttt ggaagaatcg cctaaggccc agtctgagtc
     2641 acaggagcca atggccactg ggcagcctct ggagccttcc ctggtccaga gatcatccaa
     2701 tgcactgtta aaggagctga cgtcaaagaa agatatatca aaatgccaag attcatcagc
     2761 cttttcccct cctacccagc acgttattgc ccagcctcct gtgacgatct tcatggaaac
     2821 cattcgcttc ttgatgaagg tcaacgctgt acagtttgtg cacagagtgc tggcacatga
     2881 gctgctgtgc ccccaaggag gacccagttg tgagtattat ctggttctgg cccagacaca
     2941 tcttctcaag aaggactttg ccaagacaga ggagtacctg cagcaagcag cacagatgga
     3001 ctacctgaac cctaatgtct ggggtgtgaa gggccatctc tacttcctga gtggaaacca
     3061 tgctgaggcc aaagaatgct atgagagaac tattagtttt gtagttgatg cttctgagat
     3121 gcacttcatc ttcctacgcc tgggacacat ctacttggaa gagaaagagt atgaaaacgc
     3181 aaagagaacg tacatgcatg cctgtaagag atcaccttcg tgcctcactt ggctaggact
     3241 agggattgcc tgttaccggc tggaggagct cacggaagct gaggatgctc tgtctgaagc
     3301 caacgcgttg aataactgca acgccgaagt gtgggcctat ctggctctgg tctgcctgaa
     3361 agttggacga caactggaag cagagcaggc ttataagtac acaataaagc tgaaactgaa
     3421 agaccaggct ctacttgaag aaatccacac tgtacaggaa atggttggtt ttggaaatcc
     3481 atctttctga cagccttaca agtggatatt taatatggag ccccaagcac gtttcctttc
     3541 caaactgtca ccagatgaag cctgcccatc cccacccctt tagttcacat taaaaaaata
     3601 taaaccccaa actcaaaatt t
//
