LOCUS       NM_172447               3245 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus cilia and flagella associated protein 69 (Cfap69),
            mRNA.
ACCESSION   NM_172447
VERSION     NM_172447.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Qu,R., Zhang,Z., Wu,L., Li,Q., Mu,J., Zhao,L., Yan,Z., Wang,W.,
            Zeng,Y., Liu,R., Dong,J., Li,Q., Sun,X., Wang,L., Sang,Q., Chen,B.
            and Kuang,Y.
  TITLE     ADGB variants cause asthenozoospermia and male infertility
  JOURNAL   Hum Genet 142 (6), 735-748 (2023)
   PUBMED   36995441
REFERENCE   3  (bases 1 to 3245)
  AUTHORS   Miyata,H., Morohoshi,A. and Ikawa,M.
  TITLE     Analysis of the sperm flagellar axoneme using gene-modified mice
  JOURNAL   Exp Anim 69 (4), 374-381 (2020)
   PUBMED   32554934
  REMARK    Review article
REFERENCE   4  (bases 1 to 3245)
  AUTHORS   He,X., Li,W., Wu,H., Lv,M., Liu,W., Liu,C., Zhu,F., Li,C., Fang,Y.,
            Yang,C., Cheng,H., Zhang,J., Tan,J., Chen,T., Tang,D., Song,B.,
            Wang,X., Zha,X., Wang,H., Wei,Z., Yang,S., Saiyin,H., Zhou,P.,
            Jin,L., Wang,J., Zhang,Z., Zhang,F. and Cao,Y.
  TITLE     Novel homozygous CFAP69 mutations in humans and mice cause severe
            asthenoteratospermia with multiple morphological abnormalities of
            the sperm flagella
  JOURNAL   J Med Genet 56 (2), 96-103 (2019)
   PUBMED   30415212
  REMARK    GeneRIF: Homozygous loss-of-function mutations of CFAP69 are
            associated with abnormalities of the sperm flagella and severe
            asthenoteratospermia.
REFERENCE   5  (bases 1 to 3245)
  AUTHORS   Dong,F.N., Amiri-Yekta,A., Martinez,G., Saut,A., Tek,J.,
            Stouvenel,L., Lores,P., Karaouzene,T., Thierry-Mieg,N., Satre,V.,
            Brouillet,S., Daneshipour,A., Hosseini,S.H., Bonhivers,M.,
            Gourabi,H., Dulioust,E., Arnoult,C., Toure,A., Ray,P.F., Zhao,H.
            and Coutton,C.
  TITLE     Absence of CFAP69 Causes Male Infertility due to Multiple
            Morphological Abnormalities of the Flagella in Human and Mouse
  JOURNAL   Am J Hum Genet 102 (4), 636-648 (2018)
   PUBMED   29606301
  REMARK    GeneRIF: CFAP69 is necessary for flagellum assembly/stability and
            that in both humans and mice
REFERENCE   6  (bases 1 to 3245)
  AUTHORS   Talaga,A.K., Dong,F.N., Reisert,J. and Zhao,H.
  TITLE     Cilia- and Flagella-Associated Protein 69 Regulates Olfactory
            Transduction Kinetics in Mice
  JOURNAL   J Neurosci 37 (23), 5699-5710 (2017)
   PUBMED   28495971
  REMARK    GeneRIF: Therefore, the role of CFAP69 in the olfactory system
            seems to be to allow the olfactory transduction machinery to work
            at a precisely regulated range of response kinetics for robust
            olfactory behavior.SIGNIFICANCE STATEMENT Sensory receptor cells
            are generally thought to evolve to respond to sensory cues as fast
            as they can.
REFERENCE   7  (bases 1 to 3245)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   8  (bases 1 to 3245)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   9  (bases 1 to 3245)
  AUTHORS   McClintock,T.S., Glasser,C.E., Bose,S.C. and Bergman,D.A.
  TITLE     Tissue expression patterns identify mouse cilia genes
  JOURNAL   Physiol Genomics 32 (2), 198-206 (2008)
   PUBMED   17971504
  REMARK    GeneRIF: A330021E22Rik is most abundantly expressed in tissues rich
            in highly ciliated cells, such as olfactory sensory neurons, and is
            predicted to be important to cilia.
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AK039313.1.
            
            On Apr 6, 2007 this sequence version replaced NM_172447.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK039313.1, BC054079.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3245              AK039313.1         2-3246
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 3.07 cM"
     gene            1..3245
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /note="cilia and flagella associated protein 69"
                     /db_xref="GeneID:207686"
                     /db_xref="MGI:MGI:2443778"
     exon            1..325
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             203..3031
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /codon_start=1
                     /product="cilia- and flagella-associated protein 69"
                     /protein_id="NP_766035.1"
                     /db_xref="CCDS:CCDS19075.1"
                     /db_xref="GeneID:207686"
                     /db_xref="MGI:MGI:2443778"
                     /translation="MSTAEASATTADAAEAGGRTKTGSPRTIPVFGVAIPDGEMQGIF
                     KPMDLNRIIKVLEEEDKDVSEEKQLNYIKKLIHCYQNGFPLRDLAQIFKILSLCAEKI
                     EKQPCFVEPASDIIKLCGLPFLKKKVSDEITYTEDTANSFALLGELMKIPSSALRIQI
                     CKCIVDFYHAEPLKKHIPGYQQVSSSYKIKMVEVGGLAKAMVQSALLLENQLVEKLWV
                     LKVLQHLSTSGVNCTLMVKAQAASGICAHLNDPDPSGQLLFRSSEVLWNLLEKSSKEE
                     IIQQLSNLECLLALKEVFKNLFVRGHSHYERQLRNDILVITTIIAQNPGAPMIECGFT
                     RDLILFATFNEVKSQNPLVKSLKLFNCYEDFELKKLLFNIIVILCKDLATVQLLIDGK
                     VILALFTYVKKPERLKIMEWSAAQYEELQLHAIATLSSVAPLLIEEYMSCHGNSRILA
                     FLEWCGHEDSYFIHGNSFHGTGGRGNKFAQMRYTLRLLRAMVYLEDETVNKDLCERGV
                     IHQLIEIFKTMMSRPAEKEEAIALEIQSDTLLILSGLCENYIQRKEMFGTEGVDIVLH
                     VMKTDPKNLQRGLGYNVLLFSTLDSIWCCILGCYDSEDYFLEKEGIFLLLDILALNQK
                     KFCNLILGIMVEFCDNPKTSAHVNAWRGKKDLTAASLLIKLWRKEEKELGVKRDRNGK
                     IVDTKRPLFTSFQEEQKTMPLPANCPSIAVMDVAENIRAKIYAVLDKLDFENLPGLSA
                     EDFVTLCIIHRYFDFKIGEIWNEVSEEIKLEKLRLVTTDEKSLNSIIAATENIGKMVA
                     SLQSEMIENQALQDVQNEQRVYAKIQATHKQREQANKSWENFLARTSNAKTLKKAKKL
                     QEKAIESSRYTERPQNATFHQTVIKGLNTTVPSGRVVTVQSTPTRLLGGPLADTDIAL
                     KKLPIRGGALQRVKVKPPLNDPKKSIPT"
     exon            326..385
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            386..451
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            452..561
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            562..638
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            639..737
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            738..887
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1065
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1189
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1238
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1360
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1577
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1742
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1861
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..1980
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2062
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2255
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2256..2393
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..2470
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2471..2668
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2669..2755
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2756..2860
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2861..3245
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3051..3056
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3072
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /note="major polyA site"
     regulatory      3118..3123
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3132
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
     polyA_site      3152
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
     regulatory      3161..3166
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
                     /note="hexamer: AATACA"
     polyA_site      3186
                     /gene="Cfap69"
                     /gene_synonym="4921525K03; A330021E22Rik"
ORIGIN      
        1 gttagcaact ggagcgcgct gggtgccttg gtcctcctgt gggcactaac agcagttgga
       61 tgtccgctat gttgctgcgg cgggggttac gacagaactt tcggggtctt tctcctccct
      121 agctcagcag cgtgttttct gccagggctc gtgggtccgt ggctgcggac gcacctaaga
      181 cctcaggctc ccaccccgaa ccatgtccac cgcggaggcc tcggccacca cggccgacgc
      241 cgccgaggcg ggcggcagga cgaagaccgg cagtccccgc acaatcccgg tgtttggggt
      301 ggcgatcccg gatggggaga tgcagggtat tttcaagcct atggacctta atcgtatcat
      361 caaagtcctt gaagaggagg ataaggatgt ctcagaagaa aaacaactta attatatcaa
      421 gaaactgata cactgctatc agaatggatt tcctctaaga gatttggcac agatatttaa
      481 aattctgagt ctgtgtgcag aaaaaattga aaagcagccc tgttttgtag aacctgcatc
      541 tgacatcata aaattatgtg gcttgccatt tttgaaaaag aaagtatcgg atgaaataac
      601 ttacactgaa gatactgcta attcatttgc acttctgggt gagttaatga aaataccaag
      661 ttctgcatta aggatacaaa tttgtaaatg tattgttgac ttctatcatg cagaaccatt
      721 aaagaaacat attccaggct accagcaggt gagttcatcc tacaagatta aaatggtaga
      781 ggtcggtggc ttggcgaagg caatggtcca gtcggcactc ctgcttgaga atcagcttgt
      841 cgagaaactc tgggttctga aagtcctgca gcatctctct acttctggag ttaattgcac
      901 tttaatggtg aaagcccaag cagccagcgg aatctgtgct cacctcaatg acccagatcc
      961 ctcaggacag cttttatttc gttcatcaga agtactttgg aatttactgg aaaaatcttc
     1021 aaaggaggaa attatacaac agcttagtaa cttggaatgc ctgctggctt tgaaagaagt
     1081 atttaaaaac ctctttgtga gggggcatag tcactatgag cgtcagctta gaaatgatat
     1141 attagttatc accacaatta tagctcagaa ccctggagca ccgatgattg aatgtggctt
     1201 taccagggat ttgatactat ttgcaacttt taatgaagtt aaaagtcaaa accccttggt
     1261 aaaaagtctt aagcttttta attgctatga agattttgag ctgaagaaat tgttattcaa
     1321 tataattgtg atcttatgta aagatttagc tactgtacag ctattaatcg atggcaaagt
     1381 tattttggct ttgtttacct atgtgaaaaa gcctgagaga ctcaagatca tggagtggtc
     1441 tgcagcacag tatgaagaac tacaactgca tgcgattgcc accttgtcct ctgtggctcc
     1501 tttgttaata gaagagtata tgtcatgcca cggcaattct cggatccttg catttctgga
     1561 gtggtgtggg catgaggatt cctacttcat tcatggtaac agtttccatg gtacaggcgg
     1621 ccgtggaaac aagttcgccc agatgcgtta cactctacgg ctcctcagag ccatggttta
     1681 tctggaagat gagactgtga acaaggacct ttgtgagaga ggggttattc atcaactgat
     1741 agaaattttt aaaactatga tgagcagacc tgctgagaag gaagaggcca ttgccttaga
     1801 aatccagtct gacacattac ttattctgtc aggtctttgt gaaaattata tccaaaggaa
     1861 ggaaatgttt ggtactgaag gagttgacat agttcttcat gtaatgaaaa cagaccccaa
     1921 gaatttacag aggggtttag gctacaatgt ccttcttttc agtacgttag acagcatttg
     1981 gtgctgcatt ttgggatgtt atgattctga agattatttt cttgaaaagg aagggatttt
     2041 tctccttttg gatatactag cattgaacca aaaaaaattc tgtaatctga ttcttggaat
     2101 aatggttgaa ttttgtgata atcccaaaac ttctgctcat gtcaatgctt ggcgtgggaa
     2161 gaaagacctg actgctgcca gccttttaat taaactgtgg agaaaagagg aaaaggagct
     2221 aggagtaaaa cgtgacagga acggcaagat tgttgataca aagagacctc tatttactag
     2281 ttttcaagaa gaacaaaaga ccatgcccct tcctgctaac tgcccatcta ttgcagttat
     2341 ggatgttgct gaaaatatca gagcaaaaat ttatgctgtg ttggataaac tagattttga
     2401 aaacttaccg ggcttatctg ccgaagattt tgtcaccctc tgtataatac atagatactt
     2461 tgattttaaa attggagaaa tatggaatga agtatctgaa gaaataaagc tggaaaagct
     2521 aagactagtc acaacagatg aaaaatcttt gaactccatt atagcagcaa cagaaaatat
     2581 tggaaagatg gttgcgtcct tgcaaagtga gatgattgaa aaccaagccc ttcaggatgt
     2641 gcagaatgaa caaagagtgt atgcaaaaat acaagctaca cataagcaga gagagcaggc
     2701 taacaagtca tgggaaaact tcttggctag gacatcaaat gctaaaacat taaagaaagc
     2761 aaagaagctt caagaaaagg ctatagaatc ctccagatat actgaacgac cacagaatgc
     2821 aacattccac caaacagtta ttaaaggtct taacacaacg gtgccttcgg gtcgggtggt
     2881 gacagtgcaa agcacaccta cccggctact gggaggacct ctggctgaca cagacatcgc
     2941 tctgaaaaaa ctgcctattc gaggaggggc cttgcagcgg gtgaaagtga agccaccact
     3001 gaatgatcct aaaaagagca tccctacatg atgtgttcca cagctcctga aataaatttt
     3061 acctgcaacg tctttgctaa atgtatattt atatacaaat gtaaggcaaa aatttagaat
     3121 aaatatttta gttaaatact ataaaaatta aagttaggat aatacacttt atttttattg
     3181 aaaacaccac tgtctttgaa aaaattattg atccttatct gaaacagtaa atttctttaa
     3241 caatt
//
