LOCUS       NM_001417877             670 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus cilia and flagella associated protein 299 (Cfap299),
            transcript variant 4, mRNA.
ACCESSION   NM_001417877
VERSION     NM_001417877.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 670)
  AUTHORS   Li,H., Dai,Y., Luo,Z. and Nie,D.
  TITLE     Cloning of a new testis-enriched gene C4orf22 and its role in cell
            cycle and apoptosis in mouse spermatogenic cells
  JOURNAL   Mol Biol Rep 46 (2), 2029-2038 (2019)
   PUBMED   30820741
REFERENCE   3  (bases 1 to 670)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 670)
  AUTHORS   Miyata,H., Castaneda,J.M., Fujihara,Y., Yu,Z., Archambeault,D.R.,
            Isotani,A., Kiyozumi,D., Kriseman,M.L., Mashiko,D., Matsumura,T.,
            Matzuk,R.M., Mori,M., Noda,T., Oji,A., Okabe,M.,
            Prunskaite-Hyyrylainen,R., Ramirez-Solis,R., Satouh,Y., Zhang,Q.,
            Ikawa,M. and Matzuk,M.M.
  TITLE     Genome engineering uncovers 54 evolutionarily conserved and
            testis-enriched genes that are not required for male fertility in
            mice
  JOURNAL   Proc Natl Acad Sci U S A 113 (28), 7704-7710 (2016)
   PUBMED   27357688
REFERENCE   5  (bases 1 to 670)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 670)
  AUTHORS   Tamplin,O.J., Cox,B.J. and Rossant,J.
  TITLE     Integrated microarray and ChIP analysis identifies multiple Foxa2
            dependent target genes in the notochord
  JOURNAL   Dev Biol 360 (2), 415-425 (2011)
   PUBMED   22008794
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC147376.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253012.2659296.1,
                                           SRR17253011.5019388.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849374,
                                           SAMN00849382 [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-159               AC147376.4         129368-129526
            160-290             AC147376.4         146294-146424
            291-355             AC147376.4         169504-169568
            356-670             AC147376.4         170503-170817
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 47.77 cM"
     gene            1..670
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /note="cilia and flagella associated protein 299"
                     /db_xref="GeneID:75784"
                     /db_xref="MGI:MGI:1916571"
     exon            1..159
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..459
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /note="isoform 4 is encoded by transcript variant 4;
                     uncharacterized protein C4orf22 homolog"
                     /codon_start=1
                     /product="cilia- and flagella-associated protein 299
                     isoform 4"
                     /protein_id="NP_001404806.1"
                     /db_xref="GeneID:75784"
                     /db_xref="MGI:MGI:1916571"
                     /translation="MDQDEGLTAVDNIVTQFNTYEDFLDSQITTVDLYYLEDESLARQ
                     LVELGYRGTGEIVKREDFEARKAAINIARQAERTQKKVLPCGPGRPYIDRNLWRTVIT
                     ASVQTGCFVLLTVRSSLPAAAAEQDIWMRRGMHP"
     exon            160..290
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            291..355
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            356..670
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      631..636
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /note="hexamer: AATAAA"
     regulatory      645..650
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      650
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /note="major polyA site"
     polyA_site      670
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtccggcccg cccacaccgg ttactaggga cgcgtcggtg tagccgcgat ggatcaggac
       61 gaggggctga cggccgtgga caacattgtc acacagttca acacctatga agacttcctg
      121 gactctcaga tcactacggt cgacctgtac tacctggagg atgaaagcct ggctcgccag
      181 ttggtggagc taggctaccg agggacagga gaaatcgtga aaagggaaga tttcgaagcg
      241 agaaaggcag ctatcaatat tgcaagacag gctgaaagaa cgcagaaaaa ggtattgccc
      301 tgtggccctg gcagacctta cattgatagg aatctctgga ggactgtgat cacagcttct
      361 gttcaaactg gttgctttgt actgcttact gtgcgaagct ccctacccgc tgcagctgct
      421 gagcaagaca tctggatgag gcgaggcatg catccttaaa aatcccgtgt tctggacagt
      481 tgaggccaca cctaggtctc cacagacgag aggctttctc atgtgtcggc tggattacgg
      541 tctcacggag gacacaggct ctgcttcagt cccactactt cttccagaat ctccagtaca
      601 gtgcacgtat gttctcaaga gcatttgtgg aataaataac aagaattaaa ttttaaatca
      661 ttcaaatcaa
//
