LOCUS       NM_001024614             893 bp    mRNA    linear   ROD 08-FEB-2025
DEFINITION  Mus musculus cilia and flagella associated protein 299 (Cfap299),
            transcript variant 1, mRNA.
ACCESSION   NM_001024614
VERSION     NM_001024614.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 893)
  AUTHORS   Li,H., Dai,Y., Luo,Z. and Nie,D.
  TITLE     Cloning of a new testis-enriched gene C4orf22 and its role in cell
            cycle and apoptosis in mouse spermatogenic cells
  JOURNAL   Mol Biol Rep 46 (2), 2029-2038 (2019)
   PUBMED   30820741
REFERENCE   3  (bases 1 to 893)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 893)
  AUTHORS   Miyata,H., Castaneda,J.M., Fujihara,Y., Yu,Z., Archambeault,D.R.,
            Isotani,A., Kiyozumi,D., Kriseman,M.L., Mashiko,D., Matsumura,T.,
            Matzuk,R.M., Mori,M., Noda,T., Oji,A., Okabe,M.,
            Prunskaite-Hyyrylainen,R., Ramirez-Solis,R., Satouh,Y., Zhang,Q.,
            Ikawa,M. and Matzuk,M.M.
  TITLE     Genome engineering uncovers 54 evolutionarily conserved and
            testis-enriched genes that are not required for male fertility in
            mice
  JOURNAL   Proc Natl Acad Sci U S A 113 (28), 7704-7710 (2016)
   PUBMED   27357688
REFERENCE   5  (bases 1 to 893)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 893)
  AUTHORS   Tamplin,O.J., Cox,B.J. and Rossant,J.
  TITLE     Integrated microarray and ChIP analysis identifies multiple Foxa2
            dependent target genes in the notochord
  JOURNAL   Dev Biol 360 (2), 415-425 (2011)
   PUBMED   22008794
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC147376.4, AC140359.2, AC151721.2 and AC132255.3.
            
            On Mar 10, 2023 this sequence version replaced NM_001024614.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC049770.1, SRR17253014.5154453.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-159               AC147376.4         129368-129526
            160-290             AC147376.4         146294-146424
            291-381             AC140359.2         146607-146697
            382-524             AC151721.2         30014-30156         c
            525-654             AC132255.3         52395-52524         c
            655-893             AC132255.3         34890-35128         c
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 47.77 cM"
     gene            1..893
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /note="cilia and flagella associated protein 299"
                     /db_xref="GeneID:75784"
                     /db_xref="MGI:MGI:1916571"
     exon            1..159
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..750
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     uncharacterized protein C4orf22 homolog"
                     /codon_start=1
                     /product="cilia- and flagella-associated protein 299
                     isoform 1"
                     /protein_id="NP_001019785.1"
                     /db_xref="CCDS:CCDS19456.1"
                     /db_xref="GeneID:75784"
                     /db_xref="MGI:MGI:1916571"
                     /translation="MDQDEGLTAVDNIVTQFNTYEDFLDSQITTVDLYYLEDESLARQ
                     LVELGYRGTGEIVKREDFEARKAAINIARQAERTQKKTLTSAGKELHDDFLKALAMRE
                     EDNRAGKLSTVIFIRDRNPHGQEVSGYIDYAHRLKSEDFEVYFNGKRRLLPRTTDMSF
                     YNWDSHIAVCNSSPNYQVIADNPEGLLFKYKRDRKILNVDPKAHPGDNSTRIPIQTDL
                     YAHVVLFDHVSRRKT"
     exon            160..290
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            291..381
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            382..524
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            525..654
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            655..893
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      870..875
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /note="hexamer: AATAAA"
     polyA_site      893
                     /gene="Cfap299"
                     /gene_synonym="1700007G11Rik; 4930428O21Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtccggcccg cccacaccgg ttactaggga cgcgtcggtg tagccgcgat ggatcaggac
       61 gaggggctga cggccgtgga caacattgtc acacagttca acacctatga agacttcctg
      121 gactctcaga tcactacggt cgacctgtac tacctggagg atgaaagcct ggctcgccag
      181 ttggtggagc taggctaccg agggacagga gaaatcgtga aaagggaaga tttcgaagcg
      241 agaaaggcag ctatcaatat tgcaagacag gctgaaagaa cgcagaaaaa gacactgaca
      301 agcgcgggga aggagctgca tgatgatttc ctgaaagccc tggcgatgag ggaagaggat
      361 aatcgtgctg ggaaactgag cacagtgatc ttcattcggg acagaaatcc tcatgggcag
      421 gaagtgtcag gatatattga ttatgctcac agactaaaat ctgaagattt tgaagtctac
      481 tttaatggga aaagaagact tcttccaagg accacagaca tgagctttta caactgggac
      541 agccatattg ctgtttgcaa ttcaagtcca aattatcaag tgattgctga caaccctgaa
      601 ggcttacttt ttaaatataa gagggacaga aaaattctca atgtggaccc aaaggctcat
      661 ccaggtgaca actccaccag gatccccatc cagacggatc tctacgcaca tgtggttctc
      721 tttgaccacg tctccagaag gaagacttaa gcaccacaca gtaaatggtt tccttattgt
      781 tcgctacatt caaatgaatt ccttggtcag aagcaaattg taatcataaa tacataaatg
      841 agttgtatag ctagaaggcg aattagaata ataaaattaa caaagacaga aaa
//
