LOCUS       NM_001115074            2791 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus cilia and flagella associated protein 221 (Cfap221),
            mRNA.
ACCESSION   NM_001115074 XM_011247979 XR_032988 XR_033084
VERSION     NM_001115074.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   McKenzie,C.W. and Lee,L.
  TITLE     Genetic interaction between central pair apparatus genes CFAP221,
            CFAP54, and SPEF2 in mouse models of primary ciliary dyskinesia
  JOURNAL   Sci Rep 10 (1), 12337 (2020)
   PUBMED   32704025
  REMARK    GeneRIF: Genetic interaction between central pair apparatus genes
            CFAP221, CFAP54, and SPEF2 in mouse models of primary ciliary
            dyskinesia.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Kazarian,E., Son,H., Sapao,P., Li,W., Zhang,Z., Strauss,J.F. and
            Teves,M.E.
  TITLE     SPAG17 Is Required for Male Germ Cell Differentiation and Fertility
  JOURNAL   Int J Mol Sci 19 (4), 1252 (2018)
   PUBMED   29690537
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2791)
  AUTHORS   McKenzie,C.W., Klonoski,J.M., Maier,T., Trujillo,G., Vitiello,P.F.,
            Huber,V.C. and Lee,L.
  TITLE     Enhanced response to pulmonary Streptococcus pneumoniae infection
            is associated with primary ciliary dyskinesia in mice lacking Pcdp1
            and Spef2
  JOURNAL   Cilia 2 (1), 18 (2013)
   PUBMED   24360193
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2791)
  AUTHORS   DiPetrillo,C.G. and Smith,E.F.
  TITLE     Pcdp1 is a central apparatus protein that binds Ca(2+)-calmodulin
            and regulates ciliary motility
  JOURNAL   J Cell Biol 189 (3), 601-612 (2010)
   PUBMED   20421426
REFERENCE   5  (bases 1 to 2791)
  AUTHORS   Lee,L., Campagna,D.R., Pinkus,J.L., Mulhern,H., Wyatt,T.A.,
            Sisson,J.H., Pavlik,J.A., Pinkus,G.S. and Fleming,M.D.
  TITLE     Primary ciliary dyskinesia in mice lacking the novel ciliary
            protein Pcdp1
  JOURNAL   Mol Cell Biol 28 (3), 949-957 (2008)
   PUBMED   18039845
  REMARK    GeneRIF: Pcdp1 plays an important role in ciliary and flagellar
            biogenesis and motility sperm flagella and the cilia of respiratory
            epithelial cells and brain ependymal cells in both mice and humans.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC165443.5 and AC124760.4.
            
            On or before Jun 20, 2019 this sequence version replaced
            XM_011247979.2, NM_001115074.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            CDS exon combination :: EF632061.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-54                AC165443.5         85160-85213         c
            55-240              AC165443.5         83053-83238         c
            241-341             AC165443.5         77411-77511         c
            342-428             AC165443.5         72997-73083         c
            429-527             AC165443.5         72711-72809         c
            528-628             AC165443.5         69695-69795         c
            629-732             AC124760.4         212338-212441       c
            733-892             AC124760.4         206115-206274       c
            893-1010            AC124760.4         204614-204731       c
            1011-1122           AC124760.4         204368-204479       c
            1123-1231           AC124760.4         204067-204175       c
            1232-1319           AC124760.4         201516-201603       c
            1320-1409           AC124760.4         200137-200226       c
            1410-1508           AC124760.4         198518-198616       c
            1509-1614           AC124760.4         196249-196354       c
            1615-1742           AC124760.4         187708-187835       c
            1743-1823           AC124760.4         186557-186637       c
            1824-1931           AC124760.4         184987-185094       c
            1932-2063           AC124760.4         184548-184679       c
            2064-2222           AC124760.4         183240-183398       c
            2223-2314           AC124760.4         181355-181446       c
            2315-2407           AC124760.4         178080-178172       c
            2408-2503           AC124760.4         176027-176122       c
            2504-2791           AC124760.4         173806-174093       c
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 52.54 cM"
     gene            1..2791
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /note="cilia and flagella associated protein 221"
                     /db_xref="GeneID:226356"
                     /db_xref="MGI:MGI:2684947"
     exon            1..54
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            55..240
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..2612
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /note="primary ciliary dyskinesia protein 1"
                     /codon_start=1
                     /product="cilia- and flagella-associated protein 221"
                     /protein_id="NP_001108546.1"
                     /db_xref="CCDS:CCDS48340.1"
                     /db_xref="GeneID:226356"
                     /db_xref="MGI:MGI:2684947"
                     /translation="MEVVKSPMQELQQAKEPFDTMSPLLLKSLVEEPKKRTEVPNHLL
                     ESRVYAKLLNNKVIQARPGIVHFGGYEIESKHQQILNIANISDEDTHLHILPPQTKYF
                     QINFEKKEHRLIPGLSLTVTITFSPDEWRYYYDCIRIHCKGDDTLLVPIHAYPVLNNL
                     DFPTFINLSDVFLGESKSYVIPLQCSCPVDFEFHITLLRSHQAFTIEPKSGIIPANGK
                     AKVTVKFTPIQYGMAQIKIQLWISQFNSQPYECVFTGTCYPNMALPLEEFKRLNTRSK
                     KVNVPLEKTTYVQFYPAPAKAKPQKLKEIDYQDLRFPADLSNPFAVATVLNQEPGKLK
                     IKELKQVLDQGDEISKTRQMKEAIFEQKVRQDILTEIENHLKWQVHLGKEHTTYRFKR
                     ELTEEWKKARAKYKQNRGDPVEGEELQRLQTEQSQKRIVRDLKGKRQEFHPNFDPLVN
                     NVWLTRHRAQRRFQQAARKIMLERRLLSMLGAIRGMDKESILRKIIQVNGKLIQGENP
                     SRGRRAHLKQEDNIWRYSLESEEVLHFAFPTDSESYNELALDGLGLVPIKSPEIQIKH
                     SYPYFTLKVPQLYKIKGYHPFSVNKSSTNYRLQKLARPLKHGAEDEVTTIITIPKKDT
                     TPLSAKPSILSMKPPEGLAMSVEYDPLYIFNPSPGLFAVKHPLTYAETLIDYHLCSHP
                     KYKYTQESHMGSSIPLTQRQFLHHTDIIPGIMNWKKFQPLVFSSMSDPSMVEATQRSD
                     WYSSVMLPIDVPAPLEDLPEEDRLETTERDLCDQGIEVMLTPEMVQVEFPMLIHRDSK
                     KEKDFKDSTQLPEKVGERVQEEMKNLRSKALNTYLILD"
     exon            241..341
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            342..428
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            429..527
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            528..628
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            629..732
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            733..892
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1010
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..1122
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1231
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1319
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1409
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1508
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1614
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1742
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1823
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1931
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1932..2063
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2064..2222
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2223..2314
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2315..2407
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2408..2503
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2504..2791
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2698..2703
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /note="hexamer: ATTAAA"
     polyA_site      2730
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
     regulatory      2767..2772
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /note="hexamer: AATAAA"
     polyA_site      2791
                     /gene="Cfap221"
                     /gene_synonym="Gm101; Pcdp1"
                     /note="major polyA site"
ORIGIN      
        1 ggcgacgctg tgagtaggtt cctgcgcgcc cgccccggaa ctatcgtctg cagggaaata
       61 acctttgact ctagacagac agttccattt ttcaggagca gatggaagtt gtgaaaagcc
      121 ccatgcagga actacaacag gcaaaagaac cctttgatac gatgtcaccc ctcctcttga
      181 agagcctggt agaggagccc aagaaaagaa cagaagtgcc taaccatctt ctggaatcca
      241 gggtttatgc caagcttctg aacaacaagg tcatccaggc caggccaggc atagtccact
      301 ttggaggcta tgagatagaa agcaagcacc aacagattct gaatatcgcc aatatttctg
      361 atgaagacac acaccttcat attttaccac cacagaccaa atactttcag atcaactttg
      421 aaaagaagga gcaccggctc atcccaggct tgtcactcac ggtcactatt acattttctc
      481 cagatgagtg gcgctactat tatgactgca tccgcattca ctgtaaggga gatgacacct
      541 tgcttgtccc cattcatgcc taccctgtct tgaataacct ggacttcccc acgtttataa
      601 atctatcaga tgtcttcctc ggagaaagca aaagctatgt gatccccttg cagtgcagct
      661 gccctgtgga tttcgaattc catatcacgt tgcttcggtc acatcaggcc tttacgattg
      721 agccaaagtc aggaataatt ccagctaatg ggaaggcaaa agtaactgtc aagttcacac
      781 ccatccagta tgggatggca cagataaaaa ttcaattgtg gatctcccag ttcaactctc
      841 agccatacga atgtgtcttc actggaacct gctaccccaa catggcctta ccattagaag
      901 agtttaaaag attaaatact cgttctaaga aagtaaatgt tcctctagaa aaaacaacgt
      961 atgtgcaatt ttacccggct ccggcaaagg caaaaccgca gaagttgaag gaaattgatt
     1021 atcaggacct gcggtttcca gctgacctgt caaatccatt tgccgtggca accgtcctaa
     1081 accaggaacc tggaaagttg aagattaagg aattaaaaca agtcctggac caaggtgacg
     1141 aaatctccaa aaccagacag atgaaggaag cgatcttcga acagaaagtc agacaggaca
     1201 tcctgacaga gatcgagaac catcttaagt ggcaggtgca ccttggaaaa gaacacacaa
     1261 cttacaggtt taaaagagag ctcactgagg agtggaaaaa agcacgcgcg aagtacaagc
     1321 aaaacagagg cgaccctgtg gaaggggagg agctccagcg actgcagacc gaacagagcc
     1381 aaaagcgcat cgtccgcgac ctgaagggga aaagacagga atttcatcct aattttgatc
     1441 ctcttgtgaa taatgtgtgg ctgaccagac accgggcaca aagacggttt cagcaagcag
     1501 ctcgcaagat catgctggag cgaaggttgc tcagtatgct gggggctatc cgtggaatgg
     1561 acaaagaaag catcttgagg aagattatcc aagtcaacgg aaaactgata caaggagaga
     1621 atccctcccg agggcggagg gcccacctga agcaggagga caacatctgg cgctactcac
     1681 tagagtctga ggaagtgctg cacttcgcct tccccacgga ctctgagagc tacaacgagt
     1741 tggctcttga tggccttgga ctagttccaa ttaaatctcc agaaattcaa atcaagcaca
     1801 gttatcccta cttcacttta aaggttccac agttatacaa aatcaaggga tatcatccat
     1861 tttctgtcaa caagtcctca acaaattaca gacttcagaa gcttgctcga cccctgaagc
     1921 atggtgctga ggatgaagtc accaccatca taaccatacc caagaaggat accacaccgc
     1981 tctcggcaaa gccctccatc ttgagtatga aaccacctga gggcttagcc atgtctgtgg
     2041 aatatgatcc gctgtatatt tttaatccca gcccaggact gtttgccgtg aagcatcctc
     2101 tgacctatgc ggagacactg atagattacc atctgtgctc tcacccaaag tacaagtaca
     2161 cccaggagtc ccacatgggg tccagcattc ctctcaccca aaggcagttt ctccatcata
     2221 cggatataat tcctggaatc atgaactgga agaagttcca gcccctggtg ttttcctcca
     2281 tgtctgaccc ctctatggtg gaagccactc agagaagtga ctggtacagt tccgtgatgc
     2341 tgcccataga tgtccctgcc ccactggagg acttaccaga agaagacaga ctggaaacta
     2401 ctgagcgaga tctctgtgat cagggcatag aagtcatgct gacgccagaa atggtccaag
     2461 tggaattccc catgctgatt cacagggact ccaagaagga gaaggatttc aaagactcca
     2521 cccagttacc agaaaaggtg ggagaaaggg tgcaggagga gatgaagaat ctgcgaagca
     2581 aagctctcaa cacgtacctg attctagact aaaacccact ggtgtgcccg ttgctctcct
     2641 tgccttcaaa ggtcactgag gtcctctgag ttcatttaca ttcaaaccac ttttggaatt
     2701 aaagtttcta gagacaagaa ttgaaatgca gatgccttgc tctaaactat attttttctt
     2761 tctgaaaata aaaatatgct gttatgatga c
//
