LOCUS       NM_008187               1318 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus cilia and flagella associated protein 20 (Cfap20),
            mRNA.
ACCESSION   NM_008187
VERSION     NM_008187.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Tai,L., Yin,G., Huang,X., Sun,F. and Zhu,Y.
  TITLE     In-cell structural insight into the stability of sperm microtubule
            doublet
  JOURNAL   Cell Discov 9 (1), 116 (2023)
   PUBMED   37989994
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Chen,Z., Shiozaki,M., Haas,K.M., Skinner,W.M., Zhao,S., Guo,C.,
            Polacco,B.J., Yu,Z., Krogan,N.J., Lishko,P.V., Kaake,R.M.,
            Vale,R.D. and Agard,D.A.
  TITLE     De novo protein identification in mammalian sperm using in situ
            cryoelectron tomography and AlphaFold2 docking
  JOURNAL   Cell 186 (23), 5041-5053 (2023)
   PUBMED   37865089
REFERENCE   3  (bases 1 to 1318)
  AUTHORS   Zhou,L., Liu,H., Liu,S., Yang,X., Dong,Y., Pan,Y., Xiao,Z.,
            Zheng,B., Sun,Y., Huang,P., Zhang,X., Hu,J., Sun,R., Feng,S.,
            Zhu,Y., Liu,M., Gui,M. and Wu,J.
  TITLE     Structures of sperm flagellar doublet microtubules expand the
            genetic spectrum of male infertility
  JOURNAL   Cell 186 (13), 2897-2910 (2023)
   PUBMED   37295417
REFERENCE   4  (bases 1 to 1318)
  AUTHORS   Ishikawa,H., Thompson,J., Yates,J.R. 3rd and Marshall,W.F.
  TITLE     Proteomic analysis of mammalian primary cilia
  JOURNAL   Curr Biol 22 (5), 414-419 (2012)
   PUBMED   22326026
REFERENCE   5  (bases 1 to 1318)
  AUTHORS   Yoshikawa,T., Piao,Y., Zhong,J., Matoba,R., Carter,M.G., Wang,Y.,
            Goldberg,I. and Ko,M.S.
  TITLE     High-throughput screen for genes predominantly expressed in the ICM
            of mouse blastocysts by whole mount in situ hybridization
  JOURNAL   Gene Expr Patterns 6 (2), 213-224 (2006)
   PUBMED   16325481
REFERENCE   6  (bases 1 to 1318)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   7  (bases 1 to 1318)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 1318)
  AUTHORS   Rijkers,T. and Ruther,U.
  TITLE     Sequence and expression pattern of an evolutionarily conserved
            transcript identified by gene trapping
  JOURNAL   Biochim Biophys Acta 1307 (3), 294-300 (1996)
   PUBMED   8688464
REFERENCE   9  (bases 1 to 1318)
  AUTHORS   MacLaren,D.C. and Clarke,S.
  TITLE     Rapid mapping of genomic P1 clones: the mouse
            L-isoaspartyl/D-aspartyl methyltransferase gene
  JOURNAL   Genomics 35 (2), 299-307 (1996)
   PUBMED   8661142
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM934726.1, BC119023.1 and CB195345.1.
            
            On Mar 21, 2009 this sequence version replaced NM_008187.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK011217.1, Z54179.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-104               BM934726.1         1-104
            105-1142            BC119023.1         1-1038
            1143-1318           CB195345.1         565-740
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 47.12 cM"
     gene            1..1318
                     /gene="Cfap20"
                     /gene_synonym="2600014O15Rik; Gtl3; T10-2A2"
                     /note="cilia and flagella associated protein 20"
                     /db_xref="GeneID:14894"
                     /db_xref="MGI:MGI:107428"
     exon            1..382
                     /gene="Cfap20"
                     /gene_synonym="2600014O15Rik; Gtl3; T10-2A2"
                     /inference="alignment:Splign:2.1.0"
     CDS             299..880
                     /gene="Cfap20"
                     /gene_synonym="2600014O15Rik; Gtl3; T10-2A2"
                     /note="gene trap locus 3 protein; UPF0468 protein C16orf80
                     homolog"
                     /codon_start=1
                     /product="cilia- and flagella-associated protein 20"
                     /protein_id="NP_032213.2"
                     /db_xref="CCDS:CCDS40445.1"
                     /db_xref="GeneID:14894"
                     /db_xref="MGI:MGI:107428"
                     /translation="MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQS
                     LVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRR
                     FRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANC
                     RIRRVYFSDRLYSEDELPAEFKLYLPVQNKAKQ"
     exon            383..462
                     /gene="Cfap20"
                     /gene_synonym="2600014O15Rik; Gtl3; T10-2A2"
                     /inference="alignment:Splign:2.1.0"
     exon            463..574
                     /gene="Cfap20"
                     /gene_synonym="2600014O15Rik; Gtl3; T10-2A2"
                     /inference="alignment:Splign:2.1.0"
     exon            575..763
                     /gene="Cfap20"
                     /gene_synonym="2600014O15Rik; Gtl3; T10-2A2"
                     /inference="alignment:Splign:2.1.0"
     exon            764..874
                     /gene="Cfap20"
                     /gene_synonym="2600014O15Rik; Gtl3; T10-2A2"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1295
                     /gene="Cfap20"
                     /gene_synonym="2600014O15Rik; Gtl3; T10-2A2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1267..1272
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cfap20"
                     /gene_synonym="2600014O15Rik; Gtl3; T10-2A2"
                     /note="hexamer: AATAAA"
     polyA_site      1295
                     /gene="Cfap20"
                     /gene_synonym="2600014O15Rik; Gtl3; T10-2A2"
                     /note="major polyA site"
ORIGIN      
        1 gcgcctccga ggggcgggcc caagcgtgcg gagggatccg tggcgggccg aggcggcgct
       61 ccgcctcctc tccggcaggc tcggcggcgc gggcggcagt tgctgtggtt tcggtgctga
      121 ggtggctcgg tgctcaccga ggcttacgga tctgcgtcac gccgccgctt cccgccgtca
      181 gtggctgcag ctctcagcgt cctaccttcg gaccggcggc acccgctctc tttcgccgct
      241 cggtgccgat ctccaatccc gggcctaggg cggcggctgc ggggccgcgg ctggcgagat
      301 gttcaagaac acgttccaga gtggcttcct ttccatcctc tacagcatcg gcagcaagcc
      361 cctgcagatc tgggacaaaa aggtacggaa tggccacatc aaaagaatca ctgacaatga
      421 catccagtcc ctggtgctag agattgaagg gacaaatgtc agtaccacat acatcacgtg
      481 tcctgcggac cccaagaaga cactggggat caaactgcct ttccttgtca tgatcatcaa
      541 aaacttgaag aaatatttta cctttgaagt ccaggtacta gatgacaaaa acgttcgtcg
      601 aaggtttcga gcaagtaact accagagtac cactcgagtc aagcccttca tctgtaccat
      661 gcccatgcgg ctggatgatg gctggaacca gattcagttc aacttgtcag atttcacccg
      721 gcgggcatat ggcacgaact acatcgagac cctgagagtg cagatccatg caaactgccg
      781 tatccgaagg gtttacttct cagacagact ctactcagaa gatgagctgc cagcagagtt
      841 caaactctat ctaccagttc agaacaaggc caaacaataa ctggatagca gctaagggga
      901 cagactctgg atgtgagtct tagtttaaaa aggaaactac atggagacca tgcaaaaaca
      961 tttaaataat ccactgtgct gtggctcgct gaccactgtg tccttgtcat ggacaccagt
     1021 gaccatgcac agttgtgttc cttgcattct aagtcagtgg agggaagcag agctttgagt
     1081 gacacacgag tgttggggtt tcctgctgcc tgccctggcc cgtgagagac gcacgcaaca
     1141 aacggaagaa ttagagacgt cctacaaggc agattcagac caatatttct tcgtatagtt
     1201 tattctttct tttatatatt atctaaatat atgtatatgt tgtgtaatac acataatctg
     1261 gaaatgaata aaatgctata ttcttggttt gtaaaaaaaa aaaaaaaaaa aaaaaaaa
//
