LOCUS       NM_146213               2433 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus carboxylesterase 4A (Ces4a), mRNA.
ACCESSION   NM_146213
VERSION     NM_146213.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 2433)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 2433)
  AUTHORS   Holmes,R.S., Wright,M.W., Laulederkind,S.J., Cox,L.A., Hosokawa,M.,
            Imai,T., Ishibashi,S., Lehner,R., Miyazaki,M., Perkins,E.J.,
            Potter,P.M., Redinbo,M.R., Robert,J., Satoh,T., Yamashita,T.,
            Yan,B., Yokoi,T., Zechner,R. and Maltais,L.J.
  TITLE     Recommended nomenclature for five mammalian carboxylesterase gene
            families: human, mouse, and rat genes and proteins
  JOURNAL   Mamm Genome 21 (9-10), 427-441 (2010)
   PUBMED   20931200
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164311.4, AK037191.1 and AV235376.1.
            
            On Jun 4, 2009 this sequence version replaced NM_146213.1.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus. The use of an alternative upstream start codon, which
            appears to be rodent-specific, would result in a protein that is 7
            aa longer at the N-terminal.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026374.1, AK037191.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849375,
                                           SAMN00849379 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                AC164311.4         148322-148399
            79-2368             AK037191.1         2-2291
            2369-2433           AV235376.1         252-316
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 53.04 cM"
     gene            1..2433
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /note="carboxylesterase 4A"
                     /db_xref="GeneID:234677"
                     /db_xref="MGI:MGI:2384581"
     exon            1..231
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..1844
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /note="carboxylesterase 8 (putative)"
                     /codon_start=1
                     /product="carboxylesterase 4A precursor"
                     /protein_id="NP_666325.2"
                     /db_xref="CCDS:CCDS22591.2"
                     /db_xref="GeneID:234677"
                     /db_xref="MGI:MGI:2384581"
                     /translation="MAEAGWNMKWILGLSLTLCLVVQTALGALHTKEPLLITKHGILQ
                     GKQVHVGDTPIQVFLGIPFSKPPVGTRRFAPPEPPLPWNGIRDATTYPPSCLQESWGQ
                     ILSMYLNTRKQYKWLHFSEDCLYLNVYAPVLAPGAPLLPVMVWFPGGAFLAGSASTYE
                     GSELAARGKVVLVFLQYRLGILGFFSTGNSHARGNWGLLDQIAALRWVQENIEAFGGD
                     PDSVTLFGQSAGAMSVSGLMVSPLAQGLFHQAISQSGTAVLKAFITPDPLTSAKKIAH
                     LAGCDHNSTKVMVECLRSLSAEEVTLVSKRMSFFQANSHKDPKEIVWFLSPVVDGVVF
                     PEDPVVLLTRGQVKPVPYLLGVNNAEFEWNLPFLMKIQLNQRMMNQTNINKILWSFNV
                     LLNITKEQVPVVAREYLNDAISNHDWKMFRNRLIDLIGDATFVYATLQAARYHRDAGS
                     PVYLYEFKHHASSGIIIKPYNGADHGDELSYLFGSPFSKGSSAGEEKEFSLQMMKYWA
                     NFAHTGNPNGEKLPYWPRFDKDEKYLQLDFDTRVGVKLKEKKMAFWSQLRQSQKP"
     exon            232..433
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     exon            434..575
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     exon            576..709
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     exon            710..863
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     exon            864..968
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1073
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1112
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1253
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1334
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1491
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     exon            1492..1620
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1693
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..2433
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2412..2417
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /note="hexamer: AATAAA"
     polyA_site      2433
                     /gene="Ces4a"
                     /gene_synonym="Ces8"
                     /note="major polyA site"
ORIGIN      
        1 ggctccacag ctggcgagaa gctggtcata actcctccag ccccagcctc tgatgatggg
       61 aatcaagaag ataaaggcag aactttctgg tgcttccacg ctgcgctcag agctcccttc
      121 tgaccccaga agtgtaggca tcactgcagc ccatggcaga agctggctgg aacatgaagt
      181 ggattctggg cttgagcctc accctgtgcc tggtagtcca gacagcctta ggtgctttgc
      241 acaccaagga acctctcctg atcaccaaac acgggatcct ccaaggaaag caagtgcatg
      301 tgggagacac acccatccaa gtcttcctgg ggatcccctt ctctaaacct ccggtgggca
      361 cccgcaggtt tgctcctcca gagcccccgc taccctggaa cggcatcaga gatgccacca
      421 cctacccacc ttcgtgcctt caggaatcct ggggacagat attatccatg taccttaaca
      481 ctcggaaaca gtataaatgg ctgcatttca gtgaggactg cctctacctg aatgtgtatg
      541 ccccagtact cgcccctggg gcccctctgc tgccagtgat ggtttggttt cctggaggtg
      601 ccttcctcgc cggttcggct tccacctacg aaggctcaga gttggcggcc cgtgggaaag
      661 tggtgctggt gtttctgcag tacaggctgg gcatcctggg cttcttcagc accggcaaca
      721 gccatgcccg cgggaattgg gggctgctgg accagatagc tgctttgcgg tgggtgcagg
      781 agaacatcga agcctttggt ggagacccgg acagtgtaac actatttggt cagtcggcgg
      841 gagcgatgag cgtctcagga ctgatggtat cacccctagc ccagggtctg ttccatcaag
      901 ccatctccca gagtggtact gcggtcctga aagccttcat cactcccgat ccactgacgt
      961 cagccaagaa gatcgctcac ctggctggct gcgaccacaa tagcacgaag gtcatggtgg
     1021 aatgcctgag gtctctctca gcagaggagg tgacgcttgt ttccaagagg atgtcattct
     1081 ttcaggctaa ctcccataaa gatccaaaag agatcgtgtg gttcctgagc ccagtggtgg
     1141 atggtgtggt gttcccagaa gatcctgtgg tgctcctgac ccgtgggcag gttaaacctg
     1201 tgccctacct cctaggtgtc aacaacgcag agttcgagtg gaacttacct tttctcatga
     1261 agattcagct aaatcagcgt atgatgaacc aaaccaacat caacaaaata ctctggagtt
     1321 tcaacgttct gctgaatatc actaaggagc aggtcccagt ggtggcaaga gagtatttga
     1381 atgacgctat cagtaaccat gactggaaga tgttccgaaa tcgcctgata gacctaatcg
     1441 gagatgccac ctttgtctac gccacactcc aagctgcgag gtaccaccga gatgctggct
     1501 cccccgtcta tctgtacgag ttcaagcacc atgcttcttc gggcatcatc atcaaacctt
     1561 acaatggagc agaccacgga gacgagcttt cctatctctt tggcagccct ttctccaaag
     1621 gctcatctgc aggtgaggaa aaggaattca gcctccagat gatgaaatac tgggccaact
     1681 ttgctcacac tgggaacccc aatggcgaaa agctgcccta ctggccacgc tttgacaagg
     1741 atgagaagta cctgcagttg gacttcgata caagagtggg tgtgaagctc aaggagaaaa
     1801 agatggcttt ctggagtcag cttcgccaat ctcagaaacc ttagaagcac cagctgtact
     1861 gagagtgacc aagcaagagg gagctatgct accattctgc ctcaaggaga ctggccctgg
     1921 atacatctga ggacaggagt cctcttgact caatctagga catgtaaata ttcctgactg
     1981 ccttcagatt ggagctggag ccctagtcca tcgtatgggg cttagtacta ccagtctagc
     2041 ctgatgtccc ataacacctt atagctcatt atctaatgac ctataaccaa gccagggcct
     2101 ttcatccccc gccccacctt agtcccatct cagaaccatc tcttttctcc atttcataag
     2161 acctcttatc cttcatgatg tatctgcagc cctctcttat ggaaagttca cacactgcca
     2221 cttcctgtca ttgtaccctg ccgtggttga tcatctactc tgttcaactt gcttacttat
     2281 gatgatcttg aggcccccag acaatccatt catgatatgg tgaaaagctt tgatggcttg
     2341 ggatctgtat cctcttctca cactcacctc ccccggggtg cttccaaagc ctgtacctag
     2401 agattgtcct caataaaaac tatgttgact tga
//
