LOCUS       NM_026058               3668 bp    mRNA    linear   ROD 01-OCT-2024
DEFINITION  Mus musculus ceramide synthase 4 (Cers4), mRNA.
ACCESSION   NM_026058
VERSION     NM_026058.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Wofford,W., Kim,J., Kim,D., Janneh,A.H., Lee,H.G., Atilgan,F.C.,
            Oleinik,N., Kassir,M.F., Saatci,O., Chakraborty,P., Tokat,U.M.,
            Gencer,S., Howley,B., Howe,P., Mehrotra,S., Sahin,O. and
            Ogretmen,B.
  TITLE     Alterations of ceramide synthesis induce PD-L1 internalization and
            signaling to regulate tumor metastasis and immunotherapy response
  JOURNAL   Cell Rep 43 (8), 114532 (2024)
   PUBMED   39046874
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Zhang,J., Ruiz,M., Bergh,P.O., Henricsson,M., Stojanovic,N.,
            Devkota,R., Henn,M., Bohlooly-Y,M., Hernandez-Hernandez,A.,
            Alsheimer,M., Boren,J., Pilon,M. and Shibuya,H.
  TITLE     Regulation of meiotic telomere dynamics through membrane fluidity
            promoted by AdipoR2-ELOVL2
  JOURNAL   Nat Commun 15 (1), 2315 (2024)
   PUBMED   38485951
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3668)
  AUTHORS   El-Hindi,K., Brachtendorf,S., Hartel,J.C., Oertel,S., Birod,K.,
            Merz,N., Trautmann,S., Thomas,D., Weigert,A., Schaufele,T.J.,
            Scholich,K., Schiffmann,S., Ulshofer,T., Utermohlen,O. and
            Grosch,S.
  TITLE     T-Cell-Specific CerS4 Depletion Prolonged Inflammation and Enhanced
            Tumor Burden in the AOM/DSS-Induced CAC Model
  JOURNAL   Int J Mol Sci 23 (3), 1866 (2022)
   PUBMED   35163788
  REMARK    GeneRIF: T-Cell-Specific CerS4 Depletion Prolonged Inflammation and
            Enhanced Tumor Burden in the AOM/DSS-Induced CAC Model.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3668)
  AUTHORS   Peters,F., Tellkamp,F., Brodesser,S., Wachsmuth,E., Tosetti,B.,
            Karow,U., Bloch,W., Utermohlen,O., Kronke,M. and Niessen,C.M.
  TITLE     Murine Epidermal Ceramide Synthase 4 Is a Key Regulator of Skin
            Barrier Homeostasis
  JOURNAL   J Invest Dermatol 140 (10), 1927-1937 (2020)
   PUBMED   32092351
REFERENCE   5  (bases 1 to 3668)
  AUTHORS   Gencer,S., Oleinik,N., Kim,J., Panneer Selvam,S., De Palma,R.,
            Dany,M., Nganga,R., Thomas,R.J., Senkal,C.E., Howe,P.H. and
            Ogretmen,B.
  TITLE     TGF-beta receptor I/II trafficking and signaling at primary cilia
            are inhibited by ceramide to attenuate cell migration and tumor
            metastasis
  JOURNAL   Sci Signal 10 (502) (2017)
   PUBMED   29066540
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3668)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   7  (bases 1 to 3668)
  AUTHORS   Mizutani,Y., Kihara,A. and Igarashi,Y.
  TITLE     Mammalian Lass6 and its related family members regulate synthesis
            of specific ceramides
  JOURNAL   Biochem J 390 (Pt 1), 263-271 (2005)
   PUBMED   15823095
REFERENCE   8  (bases 1 to 3668)
  AUTHORS   Thuret,S., Bhatt,L., O'Leary,D.D. and Simon,H.H.
  TITLE     Identification and developmental analysis of genes expressed by
            dopaminergic neurons of the substantia nigra pars compacta
  JOURNAL   Mol Cell Neurosci 25 (3), 394-405 (2004)
   PUBMED   15033168
REFERENCE   9  (bases 1 to 3668)
  AUTHORS   Riebeling,C., Allegood,J.C., Wang,E., Merrill,A.H. Jr. and
            Futerman,A.H.
  TITLE     Two mammalian longevity assurance gene (LAG1) family members, trh1
            and trh4, regulate dihydroceramide synthesis using different fatty
            acyl-CoA donors
  JOURNAL   J Biol Chem 278 (44), 43452-43459 (2003)
   PUBMED   12912983
  REMARK    GeneRIF: Lag1p family members trh1 and trh4 regulate
            (dihydro)ceramide synthases responsible for production of
            sphingolipids containing different fatty acids
REFERENCE   10 (bases 1 to 3668)
  AUTHORS   Venkataraman,K. and Futerman,A.H.
  TITLE     Do longevity assurance genes containing Hox domains regulate cell
            development via ceramide synthesis?
  JOURNAL   FEBS Lett 528 (1-3), 3-4 (2002)
   PUBMED   12297269
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CK617511.1, AK028937.1, AK076409.1, BB006809.2 and AV360657.2.
            
            On Jan 19, 2008 this sequence version replaced NM_026058.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028945.1, SRR1660815.219621.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                CK617511.1         77-151
            76-2553             AK028937.1         2-2479
            2554-3147           AK076409.1         1488-2081
            3148-3342           BB006809.2         461-655
            3343-3668           AV360657.2         99-424
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 2.04 cM"
     gene            1..3668
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /note="ceramide synthase 4"
                     /db_xref="GeneID:67260"
                     /db_xref="MGI:MGI:1914510"
     exon            1..186
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /inference="alignment:Splign:2.1.0"
     exon            187..360
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /inference="alignment:Splign:2.1.0"
     CDS             188..1369
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /EC_number="2.3.1.24"
                     /note="LAG1 longevity assurance homolog 4; TRAM homolog 1;
                     translocating chain-associating membrane protein homolog
                     1; LAG1 homolog, ceramide synthase 4; sphingosine
                     N-acyltransferase CERS4"
                     /codon_start=1
                     /product="ceramide synthase 4"
                     /protein_id="NP_080334.3"
                     /db_xref="CCDS:CCDS22086.1"
                     /db_xref="GeneID:67260"
                     /db_xref="MGI:MGI:1914510"
                     /translation="MSFSLSEWLWQETYWLPPNVTWAELEDRDGLVFAHPHHVLAAFP
                     VALVLVAVRIVFERFVALPLSRWMGVQDPIRRKIKPNPVLEKYFLRMKQCPEETQMVL
                     LASQCGLTLRQTQRWFRRRRNQDRPSLSKKFCEACWRFVFYLCSFVGGTSILYHESWL
                     WSPSLCWENYPHQTLNLSLYWWYLLELGFYLSLLITLPFDVKRKDFKEQVVHHFVAVG
                     LIGFSYSVNLLRIGAVVLLLHDCSDYLLEGCKILNYAHFRRGCDALFIMFALVFFYTR
                     LIFFPTQVIYTSVYDSIKNSGPFFGYYFFIVLLVMLQILHVYWFCLILRMLYSFLHKG
                     QMTEDIRSDVEEPDSSDDEPVSEGPQLKNGMARGSRVAVTNGPRSRAAACLTNGHTRA
                     T"
     exon            361..478
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /inference="alignment:Splign:2.1.0"
     exon            479..597
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /inference="alignment:Splign:2.1.0"
     exon            598..655
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /inference="alignment:Splign:2.1.0"
     exon            656..706
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /inference="alignment:Splign:2.1.0"
     exon            707..799
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /inference="alignment:Splign:2.1.0"
     exon            800..928
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1035
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1192
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..3668
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1624..1629
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /note="hexamer: AATAAA"
     polyA_site      1646
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /note="major polyA site"
     regulatory      2243..2248
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /note="hexamer: AATAAA"
     polyA_site      2265
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /note="major polyA site"
     regulatory      2709..2714
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /note="hexamer: AAGAAA"
     polyA_site      2732
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
     regulatory      3035..3040
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /note="hexamer: AATAAA"
     polyA_site      3065
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
     polyA_site      3152
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
     regulatory      3350..3355
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /note="hexamer: AATAAA"
     polyA_site      3356
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
     polyA_site      3369
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
     regulatory      3547..3552
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
                     /note="hexamer: AATAAA"
     polyA_site      3574
                     /gene="Cers4"
                     /gene_synonym="2900019C14Rik; Lass4; Trh1"
ORIGIN      
        1 accacgccca aggccccatc aaactcctcc cggaggcccc ctccggtcct atttcctccc
       61 tcgcgggtgg aggctctgta gcgaggagtt tctcggccca gagcggtctg cggcttgtct
      121 aaggcatctt cggattagct gatctccgca ccgcctcggc ctgcatcttg ctttctgaac
      181 ctccagcatg tcgttcagct tgagtgagtg gctgtggcag gagacatact ggttaccacc
      241 caatgtcaca tgggcagagc tggaggaccg tgatggcctg gtgttcgccc accctcacca
      301 cgtgcttgct gccttcccag tggctctggt cctggtggct gtgcgaattg tctttgagag
      361 atttgtggcc ttgcccctga gccggtggat gggtgtgcag gatccaatca ggagaaagat
      421 aaagcccaac ccggtgctgg agaagtactt cctcaggatg aagcagtgtc cagaggagac
      481 acagatggtc ctgctggctt cccagtgtgg cctcacgctt cggcagactc aacgctggtt
      541 caggagacgt cggaaccagg atcggccttc cttatccaag aaattctgtg aagcctgctg
      601 gaggtttgtc ttctatctgt gttccttcgt gggtggcacc tccatcctct accatgaatc
      661 atggttgtgg tcgccatcat tgtgctggga gaattaccca catcagaccc tgaatttgtc
      721 cctgtactgg tggtacctcc tggagctggg cttctacctt tcactgctga tcactttgcc
      781 ctttgatgtc aaacgcaagg acttcaagga gcaggtggtg caccattttg tggccgtggg
      841 actgataggc ttctcctaca gcgtcaactt gctccgcatt ggcgctgtgg tactgttgtt
      901 gcatgactgc tccgactacc tgctggaggg ttgtaagatt ctcaactatg cccactttag
      961 gcgagggtgc gatgctctct tcatcatgtt tgccttagtc ttcttctaca cgcgcctcat
     1021 attcttcccc acccaggtca tctacacgtc tgtgtatgac tccatcaaga actcgggccc
     1081 cttctttggc tactacttct ttattgtgct cttggtgatg ctacagattc ttcatgtgta
     1141 ctggttctgc ctcatcctgc gcatgctcta cagtttcctg cataagggtc agatgacaga
     1201 ggacattcgt agcgatgtgg aagaaccaga ctccagtgat gacgagccag tttctgaagg
     1261 tccacagctg aagaatggga tggctcgagg gtctagagta gctgttacca atggcccgcg
     1321 gagccgggca gcagcatgcc tgaccaatgg gcacacccgg gccacataga caaccttgga
     1381 ctgatgttga ggggttgccg ccagcattgt gaatattcag atgctgggct ggtgattctg
     1441 ggagacaggg aggccctacc tcaaggtagg aagaagactg atgatctgtt tcccttctgt
     1501 tcctctccct ttatcctttt gagcagcttg acagaacctg ggggagcagt ggactctgca
     1561 tctggcctga gccaacccca ggctgtagct tgggggctgt ggggagcttt ggctccaagg
     1621 accaataaaa cgtcaacagc gatgaattct ctgcgccaga gcttaagttc ccttcacagc
     1681 agttcctctc cataacgtac tctgttgaca ggattcctca ctgccacatc aagatttatg
     1741 tcaactcttg cctgtatagc accacagcat gaagatactc cctgctcctc agtcagtgcc
     1801 ttcactttgt tctacttcat agtagctctc caggacgttg gtggtagcag atacaggctg
     1861 acctgtagga aagaaattct taagcttaga tgaagcctct ctgctgctgt ccaagccaga
     1921 gggtagagca gaaacctgtg ggtgtccttg tagtcccttg cttaaagcaa cctgcagccc
     1981 accctggggt ctttatattc ccttgagaca gtggagacct aagatttggt gtgcggtcac
     2041 tacctttagg agtggtatag gaaaacatcc tcctccaaga tggaggctga gtgaccagta
     2101 ttacttaacg cagaaaccaa agccagagga tccatgtatg gttagacctt gccatctttg
     2161 ggagtatgca agaggaactg agtgtcatgg aagaatgaga atcagagagt ggggaaagag
     2221 gtcatctcag gcaacatcca aaaataaaga cttttggtac aaagagcaga gcctggcatg
     2281 gtgactcatg actgtcattt aacactaggg aggaagaagc caatagacag gaagaacata
     2341 taagcctggg atacaaagca gggcccagtt tcaaaacaaa caatgggggt gtgtgcatgt
     2401 gtgtcaagga acaggtactg cagaagagaa aactatacaa aaatccgaga tagaaacagc
     2461 tgagaaaagg aaggaaaaat gctggggcaa gaatgagggc caactctgcg tacagaacaa
     2521 ggtagtgaga tgagatagat ttttgccttc tggagaaata tgagaaaaag gaagagaact
     2581 gtggcttctg ttgagagaac aacagtttag gagaaacaga gggtgcattg gtagagaatg
     2641 catagagact tgtgaaagag acaagaagac aaagcaaccg ataggaaagg agtgggttag
     2701 tagggatcaa gaaaaaagaa gaaaaaaaga aagagaaaaa acgctgtaga tagtgcatgg
     2761 atgagaaaac tgggaaagaa caggaaaggg tattcaagac agaagacaga gggccactga
     2821 tgagagaaac agcttctaga gaacttgaga gatggaaaca aggtgtgacc acaagtctga
     2881 aatcacagta ctctgcatgc tgaggcagga agattgtagg tttgaggcca gcctgggaag
     2941 gagcaccaac ctagaaaaac aactacaaca acaaaaatgc atagagttat gtggacacaa
     3001 tggattgttt tctttctttt ttttagctgt aaagaataaa tcatgatagc tgcagaaaaa
     3061 atggatataa ctggaagtca tgtttggtag aataatatac agacaaatgc ttaatgtttt
     3121 cttttatgca aaacttagat tcaaacgata aatatttgtg tgtgtgtttg catttatata
     3181 tttataagtt atgaaaatat aggggtcatg ggccaggtat ggtggtgcac acctttaatc
     3241 ccagcacttg ggaggcaaag gcagacaaat ctgagttcaa ggccagcctg gttttccaag
     3301 caagtccagg acaggtctgt tacatagaga aaccctgtct tgaaacaaaa ataaaagaaa
     3361 gaaagataat cgagttgaag aatgtaatcc atgtgacaca gaaatggaag agtaaagaac
     3421 caaccagaaa gggacagtat gggaggagca caggaagagt aatgagtgaa tgagcgagtc
     3481 attcgtgttt atgcgaatgt caggacaaaa cacattacct tttacgtaat cataaaattt
     3541 aggaccaata aacttaaaat gcacactgac cttactaata acctcagtac agagtaatgc
     3601 tgggttctta acctggtagt tgtgatagtt aacctccatt gtcagctcga tgaaatctag
     3661 agtcatct
//
