LOCUS       NM_001164201            3251 bp    mRNA    linear   ROD 23-APR-2024
DEFINITION  Mus musculus ceramide synthase 3 (Cers3), mRNA.
ACCESSION   NM_001164201 XM_620510 XM_907730
VERSION     NM_001164201.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Zhang,J., Ruiz,M., Bergh,P.O., Henricsson,M., Stojanovic,N.,
            Devkota,R., Henn,M., Bohlooly-Y,M., Hernandez-Hernandez,A.,
            Alsheimer,M., Boren,J., Pilon,M. and Shibuya,H.
  TITLE     Regulation of meiotic telomere dynamics through membrane fluidity
            promoted by AdipoR2-ELOVL2
  JOURNAL   Nat Commun 15 (1), 2315 (2024)
   PUBMED   38485951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Kato,A., Ito,M., Sanaki,T., Okuda,T., Tsuchiya,N., Yoshimoto,R. and
            Yukioka,H.
  TITLE     Acsl1 is essential for skin barrier function through the activation
            of linoleic acid and biosynthesis of omega-O-acylceramide in mice
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1867 (2), 159085 (2022)
   PUBMED   34813948
REFERENCE   3  (bases 1 to 3251)
  AUTHORS   Miyamoto,M., Itoh,N., Sawai,M., Sassa,T. and Kihara,A.
  TITLE     Severe Skin Permeability Barrier Dysfunction in Knockout Mice
            Deficient in a Fatty Acid omega-Hydroxylase Crucial to Acylceramide
            Production
  JOURNAL   J Invest Dermatol 140 (2), 319-326 (2020)
   PUBMED   31356814
REFERENCE   4  (bases 1 to 3251)
  AUTHORS   Oberbeck,N., Pham,V.C., Webster,J.D., Reja,R., Huang,C.S.,
            Zhang,Y., Roose-Girma,M., Warming,S., Li,Q., Birnberg,A., Wong,W.,
            Sandoval,W., Komuves,L.G., Yu,K., Dugger,D.L., Maltzman,A.,
            Newton,K. and Dixit,V.M.
  TITLE     The RIPK4-IRF6 signalling axis safeguards epidermal differentiation
            and barrier function
  JOURNAL   Nature 574 (7777), 249-253 (2019)
   PUBMED   31578523
REFERENCE   5  (bases 1 to 3251)
  AUTHORS   Pichery,M., Huchenq,A., Sandhoff,R., Severino-Freire,M.,
            Zaafouri,S., Opalka,L., Levade,T., Soldan,V., Bertrand-Michel,J.,
            Lhuillier,E., Serre,G., Maruani,A., Mazereeuw-Hautier,J. and
            Jonca,N.
  TITLE     PNPLA1 defects in patients with autosomal recessive congenital
            ichthyosis and KO mice sustain PNPLA1 irreplaceable function in
            epidermal omega-O-acylceramide synthesis and skin permeability
            barrier
  JOURNAL   Hum Mol Genet 26 (10), 1787-1800 (2017)
   PUBMED   28369476
REFERENCE   6  (bases 1 to 3251)
  AUTHORS   Rabionet,M., van der Spoel,A.C., Chuang,C.C., von
            Tumpling-Radosta,B., Litjens,M., Bouwmeester,D., Hellbusch,C.C.,
            Korner,C., Wiegandt,H., Gorgas,K., Platt,F.M., Grone,H.J. and
            Sandhoff,R.
  TITLE     Male germ cells require polyenoic sphingolipids with complex
            glycosylation for completion of meiosis: a link to ceramide
            synthase-3
  JOURNAL   J Biol Chem 283 (19), 13357-13369 (2008)
   PUBMED   18308723
  REMARK    GeneRIF: both glycans and the particular acyl chains of germinal
            sphingolipids are relevant for proper completion of meiosis
REFERENCE   7  (bases 1 to 3251)
  AUTHORS   Mizutani,Y., Kihara,A. and Igarashi,Y.
  TITLE     LASS3 (longevity assurance homologue 3) is a mainly testis-specific
            (dihydro)ceramide synthase with relatively broad substrate
            specificity
  JOURNAL   Biochem J 398 (3), 531-538 (2006)
   PUBMED   16753040
  REMARK    GeneRIF: The isolation and characterization of LASS3 and LASS3-long
            are reported.
REFERENCE   8  (bases 1 to 3251)
  AUTHORS   Riebeling,C., Allegood,J.C., Wang,E., Merrill,A.H. Jr. and
            Futerman,A.H.
  TITLE     Two mammalian longevity assurance gene (LAG1) family members, trh1
            and trh4, regulate dihydroceramide synthesis using different fatty
            acyl-CoA donors
  JOURNAL   J Biol Chem 278 (44), 43452-43459 (2003)
   PUBMED   12912983
REFERENCE   9  (bases 1 to 3251)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
REFERENCE   10 (bases 1 to 3251)
  AUTHORS   Venkataraman,K. and Futerman,A.H.
  TITLE     Do longevity assurance genes containing Hox domains regulate cell
            development via ceramide synthesis?
  JOURNAL   FEBS Lett 528 (1-3), 3-4 (2002)
   PUBMED   12297269
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC120791.13.
            
            On or before Aug 15, 2009 this sequence version replaced
            XM_907730.2, XM_620510.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ646881.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-248               AC120791.13        113724-113971
            249-422             AC120791.13        134395-134568
            423-537             AC120791.13        143810-143924
            538-656             AC120791.13        149771-149889
            657-714             AC120791.13        152014-152071
            715-765             AC120791.13        153622-153672
            766-858             AC120791.13        154997-155089
            859-987             AC120791.13        156232-156360
            988-1094            AC120791.13        159809-159915
            1095-1248           AC120791.13        166006-166159
            1249-3251           AC120791.13        191910-193912
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 36.15 cM"
     gene            1..3251
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /note="ceramide synthase 3"
                     /db_xref="GeneID:545975"
                     /db_xref="MGI:MGI:2681008"
     exon            1..248
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /inference="alignment:Splign:2.1.0"
     CDS             142..1401
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /EC_number="2.3.1.24"
                     /EC_number="2.3.1.297"
                     /EC_number="2.3.1.298"
                     /note="related to TRH3; longevity assurance homolog 3;
                     LAG1 homolog, ceramide synthase 3; dihydroceramide
                     synthase 3; LAG1 longevity assurance homolog 3;
                     sphingosine N-acyltransferase CERS3; very-long-chain
                     ceramide synthase CERS3; ultra-long-chain ceramide
                     synthase CERS3"
                     /codon_start=1
                     /product="ceramide synthase 3"
                     /protein_id="NP_001157673.1"
                     /db_xref="CCDS:CCDS52268.1"
                     /db_xref="GeneID:545975"
                     /db_xref="MGI:MGI:2681008"
                     /translation="MRCVALENKAAWNGKPLQLLPTGEEYPVQTGALGRRMFQTFRKW
                     FWSERYWLPPTIKWSDLEDHDGLVFVKASHLYITIPYAFLLMVVRYFFEKFVATPLAN
                     ALGIKKTQHKIKPNAILENFFKHSTSKPSHTDIYGLAKKCNLTERQVERWLRIRQKQN
                     KPCRLQKFQESCWRFTFYLLITMAGAVFLYDKPWAYDLWEVWNDYPRQPLLPSQYWYY
                     ILEMSFYWSLVFSLSTDIKRKDFLAHVIHHLAAISLMSFSWCANYIRSGTLVMFIHDI
                     SDIWLESAKMFSYAGWKQTCNTLFFIFTVVFFISRFIIFPFWILYCTLILPLHYLEPF
                     FSYIFLNLQLMILQGLHVYWGYFILKMLNRCIFTQNVQDVRSDNEEEEEEEEEEEAES
                     TKGKETEYLKNGLGTNRHLIANGQHGR"
     exon            249..422
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /inference="alignment:Splign:2.1.0"
     exon            423..537
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /inference="alignment:Splign:2.1.0"
     exon            538..656
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /inference="alignment:Splign:2.1.0"
     exon            657..714
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /inference="alignment:Splign:2.1.0"
     exon            715..765
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /inference="alignment:Splign:2.1.0"
     exon            766..858
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /inference="alignment:Splign:2.1.0"
     exon            859..987
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1094
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1248
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..3251
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3230..3235
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /note="hexamer: AATAAA"
     polyA_site      3251
                     /gene="Cers3"
                     /gene_synonym="4930550L11Rik; Gm488; Lass3; mCerS3; T3L"
                     /note="major polyA site"
ORIGIN      
        1 aggaagcggc atcccagctg gaagaacctg aaggtgccat ccagtagctt tgcctcacgt
       61 gactaactgc cctcagtcag aaatcttgac ttgcaacttt cgagggtggg tctctgcaag
      121 aatttcaggg cttacgtata gatgaggtgt gttgctcttg agaacaaagc tgcctggaac
      181 gggaagcctc tccagttgct ccccacaggc gaggagtatc ctgtgcagac tggcgctttg
      241 ggaagaagaa tgtttcagac gtttagaaaa tggttctggt cggagagata ctggcttcct
      301 ccaacaataa agtggtcaga cctggaagat catgatgggc ttgtcttcgt gaaagcttcc
      361 cacttataca tcacaattcc atatgccttt cttttaatgg tcgtcagata cttctttgaa
      421 aagtttgttg ctacacctct agcaaatgca cttggcatta aaaagacaca gcacaaaatt
      481 aaaccaaatg ccatcttaga gaattttttc aagcattcca caagcaaacc atcacatact
      541 gatatttatg gactggcaaa gaagtgtaac ttgacagagc gccaggttga aagatggctt
      601 aggattcggc agaagcaaaa caagccttgc aggctgcaga aattccagga gtcttgctgg
      661 cgatttacat tttacttgct gataaccatg gctggagctg tatttctgta tgacaaacca
      721 tgggcatatg acctctggga ggtttggaat gactatccca gacagccttt gctgccgtcc
      781 cagtactggt actacatttt ggagatgagt ttttattggt cactggtgtt tagcctgagc
      841 actgatatca agaggaagga cttcctcgct catgtcatcc accacctggc tgctattagt
      901 ctgatgagct tctcctggtg tgctaattac attcgcagtg ggaccctcgt gatgtttatt
      961 catgatatat ctgacatttg gctagagtct gctaagatgt tttcctatgc tggatggaag
     1021 cagacgtgta acaccctgtt cttcatcttc accgtcgtct tcttcattag ccgattcatt
     1081 atttttccat tctggattct gtactgcact ctgatcctgc ctttgcacta tctcgagccc
     1141 ttcttctcct acatcttcct caacctccaa ctcatgatcc ttcagggcct ccacgtttac
     1201 tggggttact ttatcctgaa gatgctcaac agatgcatat tcacgcagaa cgtccaggat
     1261 gtgaggagtg acaacgaaga ggaagaggaa gaggaggagg aagaggaagc agagagcacc
     1321 aagggcaaag agacagagta tttgaagaat ggccttggga ccaacaggca tctcattgcc
     1381 aatggtcagc atggccgtta gcttgaagca caatgtggcg ccaggacact gggaggccgg
     1441 aactgctcct ctatccactg ctgtggaccc acagagaccc cattttgcct tttattttat
     1501 tttattttat tttattttat tgagatggag tctcctatgt tgctcaaggt ggcccccaac
     1561 tcagtcaccc tgactgtgat aaaatgttaa gaagtggaat acccgtggat tggtcagcag
     1621 ttaagtcagg ctggtcagag ttcagagatt tctccaggca gggtcagttc gtcagttgtt
     1681 gttgtgtttc cccccttcca gaatttgaag cacttgactt tctcaggaac tcgttttctc
     1741 tgaacttttt tttttttttt ttaaaactta ccaccttgta catgctccat ggtttgacaa
     1801 atgtccaggt tacccacttt ctcctggtgg ttggccacag tagcacctca tcttccaaaa
     1861 gtgtgtttgc agtggcaaga gagcagttag agccaaagga gagggcaggc tgccagggct
     1921 cctgagccac cctgggcagc cgaggtgctt ctctgcacag ggcctctgaa ggcatctgct
     1981 ctactacctc acagagttag agtaaggact gggtgagttg acaggagtgc tcgtgtcccc
     2041 tgcaggtgtt agcgacccct cactgcacta gaattccagc tttgacaaaa gagacaaccc
     2101 tggctgaact ttgtgtgaga agctccatct taaaaatggc tcttaatgtg taagataccc
     2161 ggaggtacaa aggcccagaa gtttgtttct gtttgtgact atgttgtttc cagggataat
     2221 tctcagatga gcccagagcc ttcctggcag ggtcctggcg tagagggacg tgagtttcgg
     2281 tgtcaagctg ccctgtgctg tcagccccga tccccatcct caggctcaca gagatgccat
     2341 gggctgagac ggtcactcgg gagccactga ggagcgtggg ggggtcccgg agaggttgct
     2401 cactgagcca tattacccag gaagccagcc ccagggtggc atgcctggga gcttcaaatt
     2461 ctgaaggaac tcttagaagc ggaaagagga agccatgctg tggtctcagg atctaacaca
     2521 ttctgaccac tgcgcagtga agaacccaga aggccgccag gcaccctaag tcacaaaggt
     2581 cctgtgcgca ctaaatggtc tgagagtttc agtttttaat ttttaaaatt gcgtgttttt
     2641 aaaaccttca ggctaagcaa atacgagcaa acaaacaaaa actcttcaga attctgttcc
     2701 caacaaagta tcttgttaaa gaattgtagg aaaaacagaa aaaaaacaag ccttaaatta
     2761 atatttgttt ttatgagcac agtaccatgt gcgttatatc tgtaatgtga tctcatattt
     2821 aaaatgtctc tacttactct agggcatagt tgttgagttt attgttctta ctttggtaaa
     2881 aggtctcact aaaattcttt tatgtgtcta gttaacgaag tatttcccct tgatttattt
     2941 tggttctctt tgactacaga acattttctt gcttacagtg aaacattgtt tattgctata
     3001 ctgtccaact tctttttctg ctttgacggc agaaggtaaa actgatatgc tctcaaaatc
     3061 taagctctca aacagctgaa gcacttacac tgcagaagag gaagaggtat actttgtggc
     3121 agcattggag gctgctgtga ctagggaagg taaccccatt tcctatagca aacaagggtt
     3181 cagtaccatg cgaggtgact ttgtaaagtt ctaagccata ctgactttca ataaaacaaa
     3241 agggaatccc a
//
