LOCUS       NM_029789               2127 bp    mRNA    linear   ROD 20-SEP-2024
DEFINITION  Mus musculus ceramide synthase 2 (Cers2), transcript variant 1,
            mRNA.
ACCESSION   NM_029789
VERSION     NM_029789.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Zhu,Q., Chen,S., Funcke,J.B., Straub,L.G., Lin,Q., Zhao,S.,
            Joung,C., Zhang,Z., Kim,D.S., Li,N., Gliniak,C.M., Lee,C.,
            Cebrian-Serrano,A., Pedersen,L., Halberg,N., Gordillo,R.,
            Kusminski,C.M. and Scherer,P.E.
  TITLE     PAQR4 regulates adipocyte function and systemic metabolic health by
            mediating ceramide levels
  JOURNAL   Nat Metab 6 (7), 1347-1366 (2024)
   PUBMED   38961186
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Zhang,J., Ruiz,M., Bergh,P.O., Henricsson,M., Stojanovic,N.,
            Devkota,R., Henn,M., Bohlooly-Y,M., Hernandez-Hernandez,A.,
            Alsheimer,M., Boren,J., Pilon,M. and Shibuya,H.
  TITLE     Regulation of meiotic telomere dynamics through membrane fluidity
            promoted by AdipoR2-ELOVL2
  JOURNAL   Nat Commun 15 (1), 2315 (2024)
   PUBMED   38485951
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2127)
  AUTHORS   York,A.G., Skadow,M.H., Oh,J., Qu,R., Zhou,Q.D., Hsieh,W.Y.,
            Mowel,W.K., Brewer,J.R., Kaffe,E., Williams,K.J., Kluger,Y.,
            Smale,S.T., Crawford,J.M., Bensinger,S.J. and Flavell,R.A.
  TITLE     IL-10 constrains sphingolipid metabolism to limit inflammation
  JOURNAL   Nature 627 (8004), 628-635 (2024)
   PUBMED   38383790
REFERENCE   4  (bases 1 to 2127)
  AUTHORS   Liu,Z., Xuan,C., Han,X., Zheng,Z., Xiao,R., Bao,L. and Xu,X.
  TITLE     [LASS2/TMSG1 overexpression inhibits proliferation and promotes
            apoptosis of human lung cancer A549 cells possibly by upregulating
            ceramide and p38 MAPK to activate a signaling cascade]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 43 (2), 166-174 (2023)
   PUBMED   36946034
  REMARK    GeneRIF: [LASS2/TMSG1 overexpression inhibits proliferation and
            promotes apoptosis of human lung cancer A549 cells possibly by
            upregulating ceramide and p38 MAPK to activate a signaling
            cascade].
REFERENCE   5  (bases 1 to 2127)
  AUTHORS   Griess,K., Rieck,M., Muller,N., Karsai,G., Hartwig,S., Pelligra,A.,
            Hardt,R., Schlegel,C., Kuboth,J., Uhlemeyer,C., Trenkamp,S.,
            Jeruschke,K., Weiss,J., Peifer-Weiss,L., Xu,W., Cames,S., Yi,X.,
            Cnop,M., Beller,M., Stark,H., Kondadi,A.K., Reichert,A.S.,
            Markgraf,D., Wammers,M., Haussinger,D., Kuss,O., Lehr,S.,
            Eizirik,D., Lickert,H., Lammert,E., Roden,M., Winter,D.,
            Al-Hasani,H., Hoglinger,D., Hornemann,T., Bruning,J.C. and
            Belgardt,B.F.
  TITLE     Sphingolipid subtypes differentially control proinsulin processing
            and systemic glucose homeostasis
  JOURNAL   Nat Cell Biol 25 (1), 20-29 (2023)
   PUBMED   36543979
REFERENCE   6  (bases 1 to 2127)
  AUTHORS   Imgrund,S., Hartmann,D., Farwanah,H., Eckhardt,M., Sandhoff,R.,
            Degen,J., Gieselmann,V., Sandhoff,K. and Willecke,K.
  TITLE     Adult ceramide synthase 2 (CERS2)-deficient mice exhibit myelin
            sheath defects, cerebellar degeneration, and hepatocarcinomas
  JOURNAL   J Biol Chem 284 (48), 33549-33560 (2009)
   PUBMED   19801672
  REMARK    GeneRIF: Results indicate that CERS2 activity supports different
            biological functions: maintenance of myelin, stabilization of the
            cerebellar as well as renal histological architecture, and
            protection against hepatocarcinomas.
REFERENCE   7  (bases 1 to 2127)
  AUTHORS   Laviad,E.L., Albee,L., Pankova-Kholmyansky,I., Epstein,S., Park,H.,
            Merrill,A.H. Jr. and Futerman,A.H.
  TITLE     Characterization of ceramide synthase 2: tissue distribution,
            substrate specificity, and inhibition by sphingosine 1-phosphate
  JOURNAL   J Biol Chem 283 (9), 5677-5684 (2008)
   PUBMED   18165233
  REMARK    GeneRIF: activity of CerS2 can be regulated by another bioactive
            sphingolipid, sphingosine 1-phosphate
REFERENCE   8  (bases 1 to 2127)
  AUTHORS   Mizutani,Y., Kihara,A. and Igarashi,Y.
  TITLE     Mammalian Lass6 and its related family members regulate synthesis
            of specific ceramides
  JOURNAL   Biochem J 390 (Pt 1), 263-271 (2005)
   PUBMED   15823095
REFERENCE   9  (bases 1 to 2127)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
REFERENCE   10 (bases 1 to 2127)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140190.3, CD564737.1, AK159325.1 and BQ176425.1.
            
            On Feb 26, 2016 this sequence version replaced NM_029789.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006847.1, AY029532.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AC140190.3         201626-201660
            36-77               CD564737.1         1-42
            78-2077             AK159325.1         2-2001
            2078-2127           BQ176425.1         1-50                c
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 40.74 cM"
     gene            1..2127
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /note="ceramide synthase 2"
                     /db_xref="GeneID:76893"
                     /db_xref="MGI:MGI:1924143"
     exon            1..156
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /inference="alignment:Splign:2.1.0"
     exon            157..330
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /inference="alignment:Splign:2.1.0"
     CDS             158..1300
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /EC_number="2.3.1.24"
                     /EC_number="2.3.1.297"
                     /note="LAG1 longevity assurance homolog 2; TRAM homolog 3;
                     translocating chain-associating membrane protein homolog
                     3; LAG1 homolog, ceramide synthase 2; sphingosine
                     N-acyltransferase CERS2; very-long-chain ceramide synthase
                     CERS2; tumor metastasis-suppressor gene 1 protein"
                     /codon_start=1
                     /product="ceramide synthase 2"
                     /protein_id="NP_084065.1"
                     /db_xref="CCDS:CCDS17612.1"
                     /db_xref="GeneID:76893"
                     /db_xref="MGI:MGI:1924143"
                     /translation="MLQTLYDYFWWERLWLPVNLTWADLEDKDGRVYAKASDLYITLP
                     LALLFLVIRYFFELYVATPLAALLNVKEKTRLRAPPNATLEHFYQTSGKQPKQVEVDL
                     LSRQSGLSGRQVERWFRRRRNQDRPSLLKKFREASWRFTYYLIAFVAGMAVTVDKPWF
                     YDLRKVWEGYPIQSIIPSQYWYYMIELSFYWSLLFSIASDVKRKDFKEQIIHHVATII
                     LLCFSWFANYVRAGTLIMALHDASDYLLESAKMFNYAGWKNTCNNLFIVFAIVFIITR
                     LVIMPFWILHCTMIYPLELYPAFFGYYFFNFMMAVLQMLHIFWAYFILRMAHKFITGK
                     LIEDERSDREETESSEGEETAAGAGAKSRLLANGHPILNNNHPKND"
     exon            331..448
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /inference="alignment:Splign:2.1.0"
     exon            449..567
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /inference="alignment:Splign:2.1.0"
     exon            568..625
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /inference="alignment:Splign:2.1.0"
     exon            626..676
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /inference="alignment:Splign:2.1.0"
     exon            677..769
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /inference="alignment:Splign:2.1.0"
     exon            770..898
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1005
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1159
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..2110
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2064..2069
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /note="hexamer: ATTAAA"
     regulatory      2075..2080
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /note="hexamer: AATAAA"
     polyA_site      2083
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
                     /note="major polyA site"
     polyA_site      2103
                     /gene="Cers2"
                     /gene_synonym="0610013I17Rik; Lass2; TRH3"
ORIGIN      
        1 ggggctcggc gggcaaagga ggaaggaggg agggagccgc gggcagcgga agggggagga
       61 gaggacccgg cccagtcagt gcgcaccggc cgggcgctag aagtgggaaa cggagtagcg
      121 gcgcccgggc cggagcagct ctgcaccgga cgccgagatg ctccagacct tgtatgacta
      181 cttctggtgg gaacgactat ggctgcctgt gaacttaacc tgggctgatc tagaagacaa
      241 agatggacgt gtctatgcca aagcctcaga tctctacatc acactccccc tggccctgct
      301 ctttctcgtc attcgatact tctttgagct ttatgtggca acacccctgg ctgccctcct
      361 gaatgttaag gagaaaaccc gactgcgggc acctcccaat gccaccttgg aacatttcta
      421 ccagaccagc ggcaagcagc ccaagcaggt ggaggtagac cttttgtcac ggcagagtgg
      481 gctctctgga cgccaggtag aacgctggtt ccgccgccgc cgcaaccagg acaggcccag
      541 tcttctcaaa aagttccgag aagccagctg gagattcacg tattacctaa ttgcctttgt
      601 tgctggcatg gctgtcactg tggataaacc ctggttctat gacttaagga aagtttggga
      661 gggatatccc atacagagca tcatcccttc tcagtattgg tactacatga ttgaactttc
      721 cttctactgg tccctgctct tcagcattgc ctctgatgtc aagcgaaagg attttaagga
      781 acagatcatc caccatgtgg ccactatcat tctcctctgc ttctcctggt ttgccaatta
      841 cgtccgggca gggaccctca tcatggctct gcatgacgct tctgactacc tgctggagtc
      901 tgccaagatg tttaactacg cgggatggaa gaacacctgc aacaacctct tcattgtgtt
      961 cgccatcgtt ttcatcatca ctcggctggt tatcatgcct ttctggatcc tacactgcac
     1021 gatgatatac ccactggagc tctaccctgc cttctttggc tattatttct tcaattttat
     1081 gatggcagtg ctacagatgc tccatatctt ctgggcctac ttcattctgc gtatggccca
     1141 caagttcata actggaaagc tgatagaaga tgaacgcagt gacagagaag aaacagagag
     1201 ttcagagggg gaggagactg cagctggggc aggagcaaag agccggctcc tagctaatgg
     1261 ccaccccatc ctcaataaca atcatcctaa gaatgactga accattgtcc tagctgcctc
     1321 ccacatcaat gcatgaggcc aagaaactag ccaagcttcc ctttaggggc atttgagctc
     1381 tggggagaaa ggatatgagg agtctacact gaccttccac ttcttttttc acccagttgc
     1441 ctttaaacca gactctaaac agcctatctc caggtagaaa ggaggcttgg ccacatccac
     1501 atcctgttga gagacatagt ctcatttccc tctctacctt ctaagctacc cttcttgcta
     1561 atccttcttc agctctgagg gttggaatca taactcacat tcctcattcc taagaatttg
     1621 gagctagctg tttgcctttg actccctgac ttcaagccag ggttgtgcct tactgtccct
     1681 tttctgggcc tcattctgcc aaagctggac caaggttcca tctttctaag ctccctagct
     1741 caggccaaga aagaaagctg agctactttt aactttctct tccttaaata caagtcaatg
     1801 aagtgaagga gggtatgcat acatgcccct caccctaccc cagggaccat ggttcctagg
     1861 atctcactgc ctccctctct ggccttcctg tcccctccct tcagctatga cagctggtgt
     1921 ggagtagaag ggcaactagt tctgttattt attgaacatt tggggtttca gttgtaaagc
     1981 cacaactaca ggtaggacct gatatttcgg cgagggacca tttcagacca aaatgtactg
     2041 ttaatttttt ttaattaaag tatattaaag gttaaataaa acatgactgt gtcagactat
     2101 taagacttga aaaaaaaaaa aaaaaaa
//
