LOCUS       NM_138647               2741 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus ceramide synthase 1 (Cers1), mRNA.
ACCESSION   NM_138647
VERSION     NM_138647.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Wohlwend,M., Laurila,P.P., Goeminne,L.J.E., Lima,T., Daskalaki,I.,
            Li,X., von Alvensleben,G., Crisol,B., Mangione,R.,
            Gallart-Ayala,H., Lalou,A., Burri,O., Butler,S., Morris,J.,
            Turner,N., Ivanisevic,J. and Auwerx,J.
  TITLE     Inhibition of CERS1 in skeletal muscle exacerbates age-related
            muscle dysfunction
  JOURNAL   Elife 12, 90522 (2024)
   PUBMED   38506902
  REMARK    GeneRIF: Inhibition of CERS1 in skeletal muscle exacerbates
            age-related muscle dysfunction.
            Erratum:[Elife. 2024 May 20;13:e99846. doi: 10.7554/eLife.99846..
            PMID: 38767943]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Zhang,J., Ruiz,M., Bergh,P.O., Henricsson,M., Stojanovic,N.,
            Devkota,R., Henn,M., Bohlooly-Y,M., Hernandez-Hernandez,A.,
            Alsheimer,M., Boren,J., Pilon,M. and Shibuya,H.
  TITLE     Regulation of meiotic telomere dynamics through membrane fluidity
            promoted by AdipoR2-ELOVL2
  JOURNAL   Nat Commun 15 (1), 2315 (2024)
   PUBMED   38485951
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2741)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2741)
  AUTHORS   Blachnio-Zabielska,A.U., Roszczyc-Owsiejczuk,K., Imierska,M.,
            Pogodzinska,K., Rogalski,P., Daniluk,J. and Zabielski,P.
  TITLE     CerS1 but Not CerS5 Gene Silencing, Improves Insulin Sensitivity
            and Glucose Uptake in Skeletal Muscle
  JOURNAL   Cells 11 (2), 206 (2022)
   PUBMED   35053322
  REMARK    GeneRIF: CerS1 but Not CerS5 Gene Silencing, Improves Insulin
            Sensitivity and Glucose Uptake in Skeletal Muscle.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2741)
  AUTHORS   Chen,W., Wu,C., Chen,Y., Guo,Y., Qiu,L., Liu,Z., Sun,H., Chen,S.,
            An,Z., Zhang,Z., Li,Y. and Li,L.
  TITLE     Downregulation of ceramide synthase 1 promotes oral cancer through
            endoplasmic reticulum stress
  JOURNAL   Int J Oral Sci 13 (1), 10 (2021)
   PUBMED   33753723
  REMARK    GeneRIF: Downregulation of ceramide synthase 1 promotes oral cancer
            through endoplasmic reticulum stress.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2741)
  AUTHORS   Duchala,C.S., Shick,H.E., Garcia,J., Deweese,D.M., Sun,X.,
            Stewart,V.J. and Macklin,W.B.
  TITLE     The toppler mouse: a novel mutant exhibiting loss of Purkinje cells
  JOURNAL   J Comp Neurol 476 (2), 113-129 (2004)
   PUBMED   15248193
REFERENCE   7  (bases 1 to 2741)
  AUTHORS   Venkataraman,K., Riebeling,C., Bodennec,J., Riezman,H.,
            Allegood,J.C., Sullards,M.C., Merrill,A.H. Jr. and Futerman,A.H.
  TITLE     Upstream of growth and differentiation factor 1 (uog1), a mammalian
            homolog of the yeast longevity assurance gene 1 (LAG1), regulates
            N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a
            fumonisin B1-independent manner in mammalian cells
  JOURNAL   J Biol Chem 277 (38), 35642-35649 (2002)
   PUBMED   12105227
REFERENCE   8  (bases 1 to 2741)
  AUTHORS   Clarke,D., Elgar,G. and Clark,M.S.
  TITLE     Comparative analysis of human 19p12-13 region in Fugu and mouse
  JOURNAL   Mamm Genome 12 (6), 478-483 (2001)
   PUBMED   11353399
REFERENCE   9  (bases 1 to 2741)
  AUTHORS   Lee,S.J.
  TITLE     Expression of growth/differentiation factor 1 in the nervous
            system: conservation of a bicistronic structure
  JOURNAL   Proc Natl Acad Sci U S A 88 (10), 4250-4254 (1991)
   PUBMED   2034669
REFERENCE   10 (bases 1 to 2741)
  AUTHORS   Lee,S.J.
  TITLE     Identification of a novel member (GDF-1) of the transforming growth
            factor-beta superfamily
  JOURNAL   Mol Endocrinol 4 (7), 1034-1040 (1990)
   PUBMED   1704486
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB665603.1, M62301.1 and
            BC056973.1.
            
            On Jul 15, 2009 this sequence version replaced NM_138647.2.
            
            Summary: This gene encodes a ceramide synthase enzyme, which
            catalyzes the synthesis of ceramide, the hydrophobic moiety of
            sphingolipids. The encoded enzyme synthesizes 18-carbon (C18)
            ceramide in brain neurons. Mice lacking a functional copy of this
            gene exhibit impaired cerebellar development, locomotion and motor
            coordination. This protein is transcribed from a bicistronic mRNA,
            which also encodes growth differentiation factor 1. [provided by
            RefSeq, Jul 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC056973.1, AK053885.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            bicistronic transcript :: PMID: 2034669
            NMD candidate          :: PMID: 2034669
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                BB665603.1         1-12
            13-2723             M62301.1           1-2711
            2724-2741           BC056973.1         2678-2695
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 34.15 cM"
     gene            1..2741
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
                     /note="ceramide synthase 1"
                     /db_xref="GeneID:93898"
                     /db_xref="MGI:MGI:2136690"
     exon            1..334
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..1138
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
                     /EC_number="2.3.1.299"
                     /note="LAG1 longevity assurance homolog 1; protein UOG-1;
                     longevity assurance gene 1 protein homolog 1; LAG1
                     homolog, ceramide synthase 1; sphingoid base
                     N-stearoyltransferase CERS1"
                     /codon_start=1
                     /product="ceramide synthase 1"
                     /protein_id="NP_619588.1"
                     /db_xref="CCDS:CCDS52570.1"
                     /db_xref="GeneID:93898"
                     /db_xref="MGI:MGI:2136690"
                     /translation="MAAAAATPRLEAPEPMPSYAQMLQRSWASALAAAQGCGDCGWGL
                     ARRGLAEHAHLAAPELLLAVLCALGWTALRWAATTHIFRPLAKRCRLQPRDAARLPES
                     AWKLLFYLACWSYCAYLLLGTSYPFFHDPPSVFYDWRSGMAVPWDIAVAYLLQGSFYC
                     HSIYATVYMDSWRKDSVVMLVHHVVTLLLIASSYAFRYHNVGLLVFFLHDVSDVQLEF
                     TKLNIYFKARGGAYHRLHGLVANLGCLSFCFCWFWFRLYWFPLKVLYATCHCSLQSVP
                     DIPYYFFFNILLLLLMVMNIYWFLYIVAFAAKVLTGQMRELEDLREYDTLEAQTAKPC
                     KAEKPLRNGLVKDKLF"
     exon            335..494
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
                     /inference="alignment:Splign:2.1.0"
     exon            495..675
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
                     /inference="alignment:Splign:2.1.0"
     exon            676..837
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
                     /inference="alignment:Splign:2.1.0"
     exon            838..985
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1095
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1834
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1835..2724
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2697..2702
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
                     /note="hexamer: AACAAA"
     polyA_site      2702
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
     polyA_site      2714
                     /gene="Cers1"
                     /gene_synonym="Lass1; to; Uog-1"
                     /note="major polyA site"
ORIGIN      
        1 tgcctggctg cggcgcgtga cgcgagggcg cgcggcgact cggaccggtg caggcaacag
       61 cggagacagc ggagaattgg atagcatggc tgctgccgcg gcgaccccca ggctcgaggc
      121 gccagagccc atgccgagtt atgcgcagat gttgcaacga agctgggcct cggcgctggc
      181 ggcggctcag ggctgcgggg actgcggctg gggactggcg cgccgcggcc tggcggagca
      241 cgcgcacctg gctgcacccg agctgctgct ggccgtgctc tgcgctctgg ggtggacagc
      301 gttgcgctgg gcagccacca cacacatctt tcggcccctg gccaagcggt gtcgtctgca
      361 gcctagagat gctgccaggt tacctgagag cgcctggaag cttctgttct acttggcctg
      421 ttggagctac tgcgcttacc tgctcctggg caccagttat cctttcttcc atgacccgcc
      481 ctctgtcttc tatgactgga ggtcaggcat ggcagtgccc tgggacatcg cggtggccta
      541 tttgctgcag gggagtttct actgccactc catctatgcc accgtgtaca tggacagctg
      601 gcgtaaggac tcggtggtca tgctggtgca tcacgtggtc accctgctcc tcattgcctc
      661 ttcctacgcc ttccggtacc acaacgtagg cctcctcgtg ttcttcctgc atgacgtcag
      721 cgatgtgcag ctggagttca caaaactcaa catctacttt aaggctaggg gtggtgccta
      781 ccatcgcttg catgggctgg tggccaacct gggctgcctc agcttctgtt tctgctggtt
      841 ctggttccgc ctctactggt tcccgctcaa ggttctgtac gccacttgcc actgcagcct
      901 gcagtctgtg cctgacattc cgtactactt cttcttcaac attctgctgt tgctcctgat
      961 ggtcatgaac atctattggt tcctgtacat tgtggctttc gcagccaagg tgctgactgg
     1021 tcagatgcgt gaactggaag acttgaggga gtacgacact ctggaagctc agacagccaa
     1081 gccctgcaaa gccgagaagc cactgaggaa tggcctggtg aaggacaagc tcttctgagt
     1141 ctcttgtcct caacttcagc catccaggac tctatcccat cctacctggg atactgactc
     1201 cgcccctgga gactcgaccc agtccctgga ggtctgctcc cacccctgga ggcccggtcc
     1261 cgcctttggc ggcatggcct cgcccctagg acaatagccc cgccctaaga ttcaggatgc
     1321 tacccttctc cagggactct ggctgccagc agctccgcct ttcagatcaa ttctcgacca
     1381 cccaccttgg gactgccgcc cagtcctgcc ctctggatca gtggggtcca gacacgcccc
     1441 ctccaggacc tcaaagcacc cccgacctaa ggtcaccagc ccactggccc cagacgcagt
     1501 gggctccgct gactctcttg gacacctcct gggaggaaaa tgctccctgt ctgccatcgt
     1561 ttttgcgacc acctcctcct cctgctcttg ctgccctcga cgaccctggc ccccgcgcca
     1621 gcatccatgg gccccgctgc cgccctgctc caggttcttg ggcttcccga agcgccccgg
     1681 agcgtcccca cacaccgacc tgtgcctcct gtcatgtggc gcctattccg tcgccgcgac
     1741 ccccaggagg ccagagtggg acgccctctg cggccatgcc acgtggagga actaggggtc
     1801 gccggaaaca ttgtgcgcca catccccgac agcggtctgt cctccaggcc cgcacaaccc
     1861 gccaggacct cggggctgtg ccccgagtgg acagtcgtct ttgacctgtc gaatgtggag
     1921 cccacagagc gcccaacacg cgcgcgctta gagttgcggc tggaggctga gagtgaagat
     1981 acaggggggt gggagctaag cgtggcactg tgggccgacg cagagcatcc agggcctgag
     2041 ctgctgcgcg tgccggcgcc accaggggtg ctcctgcgcg cagacctact ggggactgca
     2101 gtagccgcca acgcatcagt gccctgtact gtgcgcctgg cgctgtcact gcaccctggg
     2161 gccactgcag cctgtgggcg cctggctgag gcctccctgc tgctggtgac gctggaccca
     2221 cgcctgtgtc ccttgccgcg attgcggcgc cacacggagc ccagggtaga agttggtcca
     2281 gtgggcactt gtcgtacccg acggttgcat gtgagcttcc gtgaggtggg ctggcaccgt
     2341 tgggtgatcg cgccgcgtgg cttcctagcc aacttctgcc agggcacgtg cgcactaccc
     2401 gaaacgctga ggggacccgg cgggccgcct gcactcaacc acgctgtgct gcgcgcgctc
     2461 atgcacgcag ctgctcccac cccgggtgca ggctcgccct gctgcgtgcc agagcgtcta
     2521 tcacccatct ccgtgctctt cttcgacaat agtgacaacg tggtcctgcg acactacgaa
     2581 gacatggtgg tggatgagtg tggctgccgt tgaccacccg ggacaccctt tcagggaccg
     2641 ccccacgcaa aagcagggac tgtttgttca tgttttattg gtgacaaaaa gcttaaaaca
     2701 aatttgacta aaaattaagt tccaaaaaaa aaaaaaaaaa a
//
