LOCUS       NM_145475               4562 bp    mRNA    linear   ROD 07-MAY-2024
DEFINITION  Mus musculus ceramide kinase (Cerk), mRNA.
ACCESSION   NM_145475 XM_907499
VERSION     NM_145475.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Dong,W., Li,Q., Lu,X., Lan,J., Qiu,Z., Wang,X., Wang,J., Zheng,X.,
            Chen,S., Zhang,C. and Jin,J.
  TITLE     Ceramide kinase-mediated C1P metabolism attenuates acute liver
            injury by inhibiting the interaction between KEAP1 and NRF2
  JOURNAL   Exp Mol Med 56 (4), 946-958 (2024)
   PUBMED   38556546
  REMARK    GeneRIF: Ceramide kinase-mediated C1P metabolism attenuates acute
            liver injury by inhibiting the interaction between KEAP1 and NRF2.
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Tanaka,A., Honda,T., Yasue,M., Yamazaki,R., Hatakeyama,H.,
            Hisaka,A., Mashimo,M., Kohama,T., Nakamura,H. and Murayama,T.
  TITLE     Effects of ceramide kinase knockout on lipopolysaccharide-treated
            sepsis-model mice: Changes in serum cytokine/chemokine levels and
            increased lethality
  JOURNAL   J Pharmacol Sci 150 (1), 1-8 (2022)
   PUBMED   35926944
  REMARK    GeneRIF: Effects of ceramide kinase knockout on
            lipopolysaccharide-treated sepsis-model mice: Changes in serum
            cytokine/chemokine levels and increased lethality.
REFERENCE   3  (bases 1 to 4562)
  AUTHORS   Tanaka,A., Anada,K., Yasue,M., Honda,T., Nakamura,H. and
            Murayama,T.
  TITLE     Ceramide kinase knockout ameliorates multiple sclerosis-like
            behaviors and demyelination in cuprizone-treated mice
  JOURNAL   Life Sci 296, 120446 (2022)
   PUBMED   35245521
  REMARK    GeneRIF: Ceramide kinase knockout ameliorates multiple
            sclerosis-like behaviors and demyelination in cuprizone-treated
            mice.
REFERENCE   4  (bases 1 to 4562)
  AUTHORS   Maus,K.D., Stephenson,D.J., Ali,A.N., MacKnight,H.P., Huang,H.J.,
            Serrats,J., Kim,M., Diegelmann,R.F. and Chalfant,C.E.
  TITLE     Ceramide kinase regulates acute wound healing by suppressing
            5-oxo-ETE biosynthesis and signaling via its receptor OXER1
  JOURNAL   J Lipid Res 63 (4), 100187 (2022)
   PUBMED   35219746
REFERENCE   5  (bases 1 to 4562)
  AUTHORS   Kukreti,H. and Amuthavalli,K.
  TITLE     MicroRNA-34a causes ceramide accumulation and effects insulin
            signaling pathway by targeting ceramide kinase (CERK) in aging
            skeletal muscle
  JOURNAL   J Cell Biochem 121 (5-6), 3070-3089 (2020)
   PUBMED   32056304
  REMARK    GeneRIF: MicroRNA-34a causes ceramide accumulation and effects
            insulin signaling pathway by targeting ceramide kinase (CERK) in
            aging skeletal muscle.
REFERENCE   6  (bases 1 to 4562)
  AUTHORS   Mitsutake,S., Yokose,U., Kato,M., Matsuoka,I., Yoo,J.M., Kim,T.J.,
            Yoo,H.S., Fujimoto,K., Ando,Y., Sugiura,M., Kohama,T. and
            Igarashi,Y.
  TITLE     The generation and behavioral analysis of ceramide kinase-null
            mice, indicating a function in cerebellar Purkinje cells
  JOURNAL   Biochem Biophys Res Commun 363 (3), 519-524 (2007)
   PUBMED   17888878
  REMARK    GeneRIF: Findings suggest that CerK is not necessary for survival
            at an individual level, but might be involved in higher brain
            function related to emotion.
REFERENCE   7  (bases 1 to 4562)
  AUTHORS   Van Overloop,H., Gijsbers,S. and Van Veldhoven,P.P.
  TITLE     Further characterization of mammalian ceramide kinase: substrate
            delivery and (stereo)specificity, tissue distribution, and
            subcellular localization studies
  JOURNAL   J Lipid Res 47 (2), 268-283 (2006)
   PUBMED   16269826
REFERENCE   8  (bases 1 to 4562)
  AUTHORS   Kim,T.J., Mitsutake,S., Kato,M. and Igarashi,Y.
  TITLE     The leucine 10 residue in the pleckstrin homology domain of
            ceramide kinase is crucial for its catalytic activity
  JOURNAL   FEBS Lett 579 (20), 4383-4388 (2005)
   PUBMED   16081073
REFERENCE   9  (bases 1 to 4562)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   10 (bases 1 to 4562)
  AUTHORS   Sugiura,M., Kono,K., Liu,H., Shimizugawa,T., Minekura,H.,
            Spiegel,S. and Kohama,T.
  TITLE     Ceramide kinase, a novel lipid kinase. Molecular cloning and
            functional characterization
  JOURNAL   J Biol Chem 277 (26), 23294-23300 (2002)
   PUBMED   11956206
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC116764.10, AK042077.1, AK052269.1 and BU756835.1.
            
            On Dec 12, 2009 this sequence version replaced NM_145475.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC094253.1, AK052269.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-635               AC116764.10        45752-46386
            636-2233            AK042077.1         227-1824
            2234-4482           AK052269.1         2000-4248
            4483-4562           BU756835.1         1-80                c
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 40.52 cM"
     gene            1..4562
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /note="ceramide kinase"
                     /db_xref="GeneID:223753"
                     /db_xref="MGI:MGI:2386052"
     exon            1..635
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     CDS             494..2089
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /EC_number="2.7.1.138"
                     /note="mCERK; acylsphingosine kinase"
                     /codon_start=1
                     /product="ceramide kinase"
                     /protein_id="NP_663450.3"
                     /db_xref="CCDS:CCDS27728.1"
                     /db_xref="GeneID:223753"
                     /db_xref="MGI:MGI:2386052"
                     /translation="MGAMGAAEPLHSVLWVKRRRCAVSLEPARALLRWWRSPEPGPSA
                     PGADARSVLVSEIIAVEEKDDCEKHASSGRWHKMENPFAFTVHRVKRVRHHRWKWARV
                     TFWSADEQLCHLWLQTLRGLLESLTSRPKHLLVFINPFGGKGQGKRIYEKTVAPLFTL
                     ASITTEIIITEHANQAKETLYEINTDSYDGIVCVGGDGMFSEVLHGVIGRTQQSAGID
                     PNHPRAVLVPSTLRIGIIPAGSTDCVCYSTVGTNDAETSALHIIIGDSLAIDVSSVHY
                     HNTLLRYSVSLLGYGFYGDLIKDSEKKRWMGLVRYDFSGLKTFLSHQYYEGTLSFLPA
                     QHTVGSPRDNKPCRAGCFVCRQSKQQLEEEEKKALYGLENAEEMEEWQVTCGKFLAIN
                     ATNMSCACPRSPGGLSPFAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQEDQFDFTFV
                     EVYRVKKFHFTSKHVEDEDNDSKEQEKQKFGKICKDRPSCTCSASRSSWNCDGEVMHS
                     PAIEVRVHCQLVRLFARGIEEES"
     exon            636..749
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     exon            750..872
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     exon            873..998
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1062
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1208
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1283
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1436
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1542
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1619
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1825
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..2034
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..4546
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3287..3292
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /note="hexamer: TATAAA"
     polyA_site      3308
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /note="major polyA site"
     regulatory      4497..4502
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /note="hexamer: AATAAA"
     polyA_site      4517
                     /gene="Cerk"
                     /gene_synonym="D330016D08Rik; mKIAA1646"
                     /note="major polyA site"
ORIGIN      
        1 cctatcgcct gcccaggacc gcccgggcaa catttctgcg ctgctgggac tccgcccgtc
       61 cctttgcagc cctccctacc ccgtcgctcg tgcagaccca gcaggaccca ggggcagcca
      121 gagcgacaga ctagtgtctt tcagggcacc cggccgctcc gaccgccctc cccacagcca
      181 ggtccctcag tcccctcctc tgacccctcc ctcccgttcc ttcctaggcc ttttctgctc
      241 ctcatcctag gctccctaga ctcctctgtt tctgattctt ctctggcccc aagtctagct
      301 tctcctgtcc tcaccctggt cgcctcctcc cgggtccccg ccaccctctc tgcgtaccgg
      361 gtgacgcagc gccgcctccc gctcccgcct ccccgcgtca ccgccgggca gactgcaggg
      421 gccgctaacg gtcccgcgcc ccccccggtg tccgcgcgcc ccgcgcccgg cggacgaggc
      481 cgcgccggcg gcgatggggg caatgggggc ggcggagccg ctgcactccg tgctgtgggt
      541 gaaacggcga cgctgtgccg tcagcctgga gcccgcgcgg gctctgctac gctggtggcg
      601 gagcccggag cccgggccct ccgcgccggg tgccgatgcc cgctcggtac tggtgtcgga
      661 gatcatcgct gttgaggaaa aagacgactg cgagaaacac gcctccagtg gccgatggca
      721 taaaatggaa aatccgttcg cattcacagt ccaccgtgtg aagcgagtac gacatcaccg
      781 ctggaagtgg gcacgggtga ccttctggag cgccgacgag cagctgtgtc acctgtggct
      841 gcagaccctc cgtgggctgc tggagagcct gacttcaaga ccgaagcact tgctggtatt
      901 catcaaccct ttcggaggga aaggtcaggg caagcgcatc tatgaaaaaa cagtggcgcc
      961 tctgtttacc ttggcttcca tcactacgga gatcatcatt acagagcatg ccaaccaagc
     1021 caaggagact ttatacgaga tcaacacaga cagctatgat ggcatcgtgt gcgtaggtgg
     1081 ggacggcatg ttcagcgagg tgctgcatgg ggtgattggg aggacgcagc agagcgctgg
     1141 tatcgacccc aatcaccccc gagccgtgct ggtgcccagt accctcagga tcggcatcat
     1201 acccgcaggg tccacagatt gtgtgtgtta ctcaacagtg ggcacaaacg acgcagagac
     1261 atcggctttg cacatcatta ttggggactc actggcaata gacgtgtcct ctgtgcacta
     1321 ccataacacg ctgctgcggt actcggtttc tctgctgggc tacggtttct acggggactt
     1381 aatcaaggac agtgaaaaga aacggtggat gggcctcgtc cggtatgatt tctcagggtt
     1441 gaagaccttt ctctctcatc agtactatga agggacactg tccttcctcc cagcacagca
     1501 cacggtggga tctccacggg acaataaacc ctgccgggct gggtgcttcg tgtgcaggca
     1561 gagcaagcaa cagctggaag aagaagagaa gaaagccctg tatggcctgg agaacgccga
     1621 ggaaatggaa gagtggcaag tgacatgtgg gaagttcctg gccatcaatg ccaccaacat
     1681 gtcctgtgct tgtcctcgga gccctggggg cctgtcccca tttgcccatc tgggagatgg
     1741 gtcttctgac ctcatcctta tccggaagtg ctccaggttc aacttcctga gattcctcat
     1801 ccggcacacg aaccaggagg accagttcga cttcactttc gttgaagttt atcgagtcaa
     1861 gaaattccac ttcacgtcga agcacgtgga agacgaggac aatgactcga aggaacaaga
     1921 gaagcagaag tttgggaaga tctgcaagga cagaccctct tgcacttgct cagcctccag
     1981 aagctcctgg aactgcgatg gcgaagtcat gcacagcccg gccattgagg tcagggtcca
     2041 ctgccagctg gtgcgcctct ttgctcgggg aatcgaggaa gagtcataag caagaacccc
     2101 aaagcccagg agctgtcggc cttgagctcg gggagtgtgg aaattactta agaaaaattc
     2161 gacagaccag ttatgttgat atatccattt gaatttagaa atttattttt gataggtaaa
     2221 tcttggtttt tagaaaagac agctttgtca acagttctgt atccatacct ggtaccctcg
     2281 gccccgcttc cgagtcgctt ttcacttctt ctcagcaaat ggcacttgct ggttccgggc
     2341 attggcttcc tagattttag ccatgccgac cgtgcatatt tcaagacagc cccttagtgg
     2401 gctacactgc aataattttt agggacatta taatatgact acgtcccagg aatcaggtta
     2461 caccatctgg tcacatgggt aaaacccggt caccttgacc cctttggtgg gacttcctct
     2521 ggaattgtcc gtggccaggt ttgggaaggt aaatagctgt cttggcctcc gctttacaca
     2581 caccttcatt tacgatcagg acgtcgctat gtggcccttg ctgcacacca caactgactc
     2641 agcgaggtcc tccgcacagc catgcctgca gagcagggcc atgtcacagc gagaagacac
     2701 ccagggtgac gtctcatcct caggatcccc cacgtctgct gccatcacag ccgcatcccg
     2761 tgagacaatt cagcaaagcg cccacttgga agcttcattc tgtcagccct ggtagcctgc
     2821 acaccctggc caaggcagtc tgaagcactg gctaagagga ggctccattt ggaatcaaag
     2881 cacttctaca gctgggtttt gaaagaggta gggggtggcg gggacagaga gtcactgaca
     2941 cacacagagg gtactcggtg agttcgatac caccctcaac ctgactgtca ccagcgaatg
     3001 gtgggtgatc tcgggtcacc cccaggtact ctggtgcaag ccttatgtct actcctggtt
     3061 ccacatccat tcttccgcac tgtggattct gttcttattc atagctttct ccctatccat
     3121 cacgtcttgg ctatttgctc tgtctccaca agggggcgct aagctgctca ggccgaagcc
     3181 ctgggcgcat gaccagtttt cctctgaagg cattcccaac ccaggggctt ctcggccaca
     3241 tactatggcc tgtgctgtgt ctccagatgg atgcatctga aatgtgtata aaagggtttt
     3301 atgtgaaatt caggcctaca cagcttaaac ttgacttgca aacggggaag ggcactgact
     3361 gttacaagag ctggttttag gttctgcact gggggaagtg tgcatctcgt gctggtccag
     3421 ttactcgcta cagaattctg caggccacac ggctttccag gtcctcttgc actgacttct
     3481 gtttcgaggc atacacaaaa tcctctgttt agtattcagt cccatccaga caaaagagca
     3541 gctgtaaagg cggccatgtt cagggaccac tttaaaggca gttgtgcggc ggtgctattt
     3601 aatgattgtc gagtagaaag ctggctagaa agagtaaatg aactagcagt ttctatccag
     3661 atctgtccct ggatgggcag agcccaaagg tgccagccta gcagctctgt gagtaggtag
     3721 gcagccctgg agcagcgtga gggccgatgc tcagttgtcc acctcaatgt ggcccctcgc
     3781 ccccggcatt ccagaggggg ctcagggccc gaggcagctg tgggtgcccc tggatctcag
     3841 gaattgcact gtcctccaag gactcaggtc ttcactgacc ttgctacatg atacccacgc
     3901 cctggtccac ccctgccgct gccagggctg gaggagagat gcagtcagag gatgctttgc
     3961 cggtgcttag cagtgcaagc cacgctcgga atctccaacc ccggcgcagt catggagcat
     4021 catgagtgtc agacacttag ggcccttgac acggctgcgc aggattgggg gggggggcgt
     4081 gctgacattc acaagcagta gacagtcttc atgatgcttt ttagatgcaa taccccaaac
     4141 ttcccgggtc ttaggtattt taggaggaaa aaacaggggt aaaggttaga cagtggcaac
     4201 ttaggatcct gttagggtct ggcccatgtt ttaactccca ctggtgcata ccaattaggc
     4261 aacagtcata agctgtaact tatttttgtt tatggaaact aagtagtggg aacggggacc
     4321 ggggagcggc tggtttccca gaggatgctt cagccaatgc aatctcctgg agctgggagc
     4381 actgactggg agcactgaca caagaatggc ccaattatgc ctcctggtct tgctggcttc
     4441 aagattagaa agtggcatat ttgatctggt atatttgaga agacaaaaaa aataataata
     4501 aagttacttt gggcagacta tggcctcccc tcatcctcat ttacaaaaaa aaaaaaaaaa
     4561 aa
//
