LOCUS       NM_001130000            7977 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus centrosomal protein 250 (Cep250), transcript variant
            4, mRNA.
ACCESSION   NM_001130000
VERSION     NM_001130000.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Kang,M., Kim,J.A., Song,M.H., Joo,S.Y., Kim,S.J., Jang,S.H.,
            Lee,H., Seong,J.K., Choi,J.Y., Gee,H.Y. and Jung,J.
  TITLE     Novel Variant in CEP250 Causes Protein Mislocalization and Leads to
            Nonsyndromic Autosomal Recessive Type of Progressive Hearing Loss
  JOURNAL   Cells 12 (18), 2328 (2023)
   PUBMED   37759551
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7977)
  AUTHORS   Xiang,L., Yang,Q.L., Xie,B.T., Zeng,H.Y., Ding,L.J., Rao,F.Q.,
            Yan,T., Lu,F., Chen,Q. and Huang,X.F.
  TITLE     Dysregulated Arginine Metabolism Is Linked to Retinal Degeneration
            in Cep250 Knockout Mice
  JOURNAL   Invest Ophthalmol Vis Sci 64 (12), 2 (2023)
   PUBMED   37656476
  REMARK    GeneRIF: Dysregulated Arginine Metabolism Is Linked to Retinal
            Degeneration in Cep250 Knockout Mice.
REFERENCE   4  (bases 1 to 7977)
  AUTHORS   Chen,C., Rong,Y., Zhuang,Y., Tang,C., Liu,Q., Lin,P., Li,D.,
            Zhao,X., Lu,F., Qu,J. and Liu,X.
  TITLE     RNA-Seq Analysis Reveals an Essential Role of the cGMP-PKG-MAPK
            Pathways in Retinal Degeneration Caused by Cep250 Deficiency
  JOURNAL   Int J Mol Sci 24 (10), 8843 (2023)
   PUBMED   37240188
  REMARK    GeneRIF: RNA-Seq Analysis Reveals an Essential Role of the
            cGMP-PKG-MAPK Pathways in Retinal Degeneration Caused by Cep250
            Deficiency.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7977)
  AUTHORS   Abu-Diab,A., Gopalakrishnan,P., Matsevich,C., de Jong,M.,
            Obolensky,A., Khalaileh,A., Salameh,M., Ejzenberg,A., Gross,M.,
            Banin,E., Sharon,D. and Khateb,S.
  TITLE     Homozygous Knockout of Cep250 Leads to a Relatively Late-Onset
            Retinal Degeneration and Sensorineural Hearing Loss in Mice
  JOURNAL   Transl Vis Sci Technol 12 (3), 3 (2023)
   PUBMED   36857066
  REMARK    GeneRIF: Homozygous Knockout of Cep250 Leads to a Relatively
            Late-Onset Retinal Degeneration and Sensorineural Hearing Loss in
            Mice.
REFERENCE   6  (bases 1 to 7977)
  AUTHORS   Kobayashi,T., Kim,S., Lin,Y.C., Inoue,T. and Dynlacht,B.D.
  TITLE     The CP110-interacting proteins Talpid3 and Cep290 play overlapping
            and distinct roles in cilia assembly
  JOURNAL   J Cell Biol 204 (2), 215-229 (2014)
   PUBMED   24421332
REFERENCE   7  (bases 1 to 7977)
  AUTHORS   Sonn,S., Jeong,Y. and Rhee,K.
  TITLE     Nip2/centrobin may be a substrate of Nek2 that is required for
            proper spindle assembly during mitosis in early mouse embryos
  JOURNAL   Mol Reprod Dev 76 (6), 587-592 (2009)
   PUBMED   19117032
REFERENCE   8  (bases 1 to 7977)
  AUTHORS   Yang,J., Adamian,M. and Li,T.
  TITLE     Rootletin interacts with C-Nap1 and may function as a physical
            linker between the pair of centrioles/basal bodies in cells
  JOURNAL   Mol Biol Cell 17 (2), 1033-1040 (2006)
   PUBMED   16339073
REFERENCE   9  (bases 1 to 7977)
  AUTHORS   Delgehyr,N., Sillibourne,J. and Bornens,M.
  TITLE     Microtubule nucleation and anchoring at the centrosome are
            independent processes linked by ninein function
  JOURNAL   J Cell Sci 118 (Pt 8), 1565-1575 (2005)
   PUBMED   15784680
REFERENCE   10 (bases 1 to 7977)
  AUTHORS   Menz,K., Radomski,N. and Jost,E.
  TITLE     INMP, a novel intranuclear matrix protein related to the family of
            intermediate filament-like proteins: molecular cloning and sequence
            analysis
  JOURNAL   Biochim Biophys Acta 1309 (1-2), 14-20 (1996)
   PUBMED   8950169
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK145562.1 and AL833786.8.
            
            Transcript Variant: This variant (4) differs in the 5' UTR and
            includes an additional in-frame segment compared to variant 1,
            resulting in a longer isoform (3) compared to isoform 1.
            
            Sequence Note: Sequence Note: This RefSeq record was created from
            transcript and genomic sequence data because no single transcript
            was available for the full length of the gene. The extent of this
            transcript is supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849375,
                              SAMN00849380 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2702              AK145562.1         2-2703
            2703-3332           AK145562.1         2705-3334
            3333-3412           AL833786.8         34049-34128
            3413-3551           AL833786.8         35946-36084
            3552-3894           AL833786.8         36531-36873
            3895-4016           AL833786.8         37707-37828
            4017-4137           AL833786.8         39024-39144
            4138-4259           AL833786.8         40211-40332
            4260-6818           AL833786.8         40928-43486
            6819-6932           AL833786.8         45283-45396
            6933-7079           AL833786.8         45557-45703
            7080-7181           AL833786.8         46031-46132
            7182-7244           AL833786.8         46984-47046
            7245-7977           AL833786.8         48880-49612
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 77.26 cM"
     gene            1..7977
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /note="centrosomal protein 250"
                     /db_xref="GeneID:16328"
                     /db_xref="MGI:MGI:108084"
     exon            1..100
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            101..383
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     CDS             201..7508
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /note="isoform 3 is encoded by transcript variant 4;
                     centrosome-associated protein CEP250; C-Nap1; 250 kDa
                     centrosomal protein; centrosomal Nek2-associated protein
                     1; intranuclear matrix protein"
                     /codon_start=1
                     /product="centrosome-associated protein CEP250 isoform 3"
                     /protein_id="NP_001123472.1"
                     /db_xref="CCDS:CCDS50772.1"
                     /db_xref="GeneID:16328"
                     /db_xref="MGI:MGI:108084"
                     /translation="METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNS
                     QEAQKRQATLVRKLQAKVLQYRSWCQDLEKRLEATGGLIPQRWESVEEPNLEQLLIRL
                     EEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRM
                     EQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTC
                     CLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLE
                     KAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQ
                     EEKLSLQQVIKAITQALASVEEEDTVTQSSGHEDSLQSDCNGLSQFDPQDPDRALTLV
                     QSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGRALREKLQKLTGERDALAG
                     QTVGLQGEVDSLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEA
                     WRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLT
                     LREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLG
                     ALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEK
                     KTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAE
                     RQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEA
                     QQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLA
                     QAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLR
                     EQQTEMEAIRAQREEERSQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLER
                     LRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQKQVEDL
                     MSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENASLALSLVEKEKRLLILQE
                     ADSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLEELEAS
                     RVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQ
                     ALGSACESRPELRGGGDSAPTLWGPDPDQNGASRLFKRWSLPTALSPEAVALALQKLH
                     QDVWKARQARDDLRDQVQKLVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGR
                     QNSLESELRDLHETAASLQSRLRQAELQKMEAQNDRELLQASKEKLSAQVEHLQACVA
                     EAQAQADAAAVLEEDLRTARSALKLKNEELESERERAQALQEQGELKVAQGKALQENL
                     ALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQIQE
                     LEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIE
                     SQRGQIQDLKKQLGTLECLALELEESHHKVESQQKMITELEGQREMQRVALTHLTLDL
                     EERSQELQAQSSQLHELENHSTHLAKELQERDQEVTSQRQQIDELQKQQEQLAQALER
                     KGQELVLQKERIQVLEDQRTLQTKILEEDLEQIKHSLRERSQELASQWQLVHERADDG
                     KSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQLQGLHRKVGETSL
                     LLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQT
                     QDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEE
                     VLGDLRAESREHEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAAL
                     RAENQYSRRQEEAAVSQAEALQEALSKAQAALQEKEQSLLEQAELSHTLEASTAALQA
                     TLDTCQASARQLEEALRIREGEIQAQALQHHEVTQHLQQELCQKKEELRQLLEKAGAR
                     RSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLREASSPRHRALPA
                     EKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMF
                     LQASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQGSQSGEVETE
                     PSPGVEEKERLTQRLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSV
                     RASRAGSLEARETMTSSPTQQDGRGSQRGSSDSVLVVELQREVALLRAQLALERKQRQ
                     DYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLEAETRKLDESLNQSLTSPGPCL
                     LHPSLDTTQNTHR"
     exon            384..440
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            441..523
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            524..689
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            690..796
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            797..1048
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1145
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1253
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1412
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1654
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1837
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..1982
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2129
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..2360
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            2361..2554
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            2555..2682
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            2683..2852
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            2853..3020
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            3021..3155
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            3156..3278
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            3279..3412
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            3413..3551
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            3552..3894
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            3895..4016
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            4017..4137
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            4138..4259
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            4260..6818
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            6819..6932
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            6933..7079
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            7080..7181
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            7182..7244
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     exon            7245..7977
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7954..7959
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /note="hexamer: AATAAA"
     polyA_site      7977
                     /gene="Cep250"
                     /gene_synonym="B230210E21Rik; Cep2; Inmp"
                     /note="major polyA site"
ORIGIN      
        1 ctggcctcca gactgagggc ggccccaggc cgggcctgca ggttgctgca gcggcctcgg
       61 tagctgcgga gcgcttcggg atccccggga accgactctg ctcaagagga ggtctaagac
      121 acgtaggaca atggttagac accctcctgc aaccagcctc tagctacaca ggagctgcgg
      181 atttgttctg tggtgcgccc atggagacag ggagccccgg gttgaacatg aagccccagt
      241 ccttgcagct ggtgctggag gggcaggtgc tggcgctgca gcagcagatg gccgagaatc
      301 aggctgcctc ctggcggaaa ctgaagaact cccaagaggc ccagaagaga caagcaaccc
      361 tcgtgaggaa gctgcaggcc aaggttttac agtaccggag ctggtgtcaa gacctggaga
      421 agcgactaga agccactggg gggctgatcc ctcagcgctg ggagagtgta gaagagccca
      481 acctggaaca gttgctgatc cgattggagg aggagcagca gaggtgtgag agtctagtgg
      541 aggtgaacac cgagcttcgg ctgcacatgg agaaagccga tgtggtgaat aaagccttgc
      601 aggaggacgt ggaaaaactg acagtggact ggagtcgggc ccgagatgag ttagtgagga
      661 aggagagcca gtggcggatg gagcaggagt tcttcaaggg ctatttgaga ggtgaacacg
      721 gtcgtcttct caatctatgg cgagaggtgg taaccttccg acgtcacttc ctgaaaatga
      781 agtcagctac tgacagagac ctgacagagc taaaggctga acacgcaagg ctttcagggt
      841 ccctgctgac ctgctgtctg cgcctgaccc tgagagcaca gtctcgggag tccagtggat
      901 ctgggagaac agaggagagt gagccagccc ggctgctgct gctcgtcgct aagacccagg
      961 cgctggagaa ggaagcccat gagaagagcc aggagttaat gcagctcaag agtcacgggg
     1021 atctagagaa ggcagagctg caggaccgag tgacggaact ctctgctctg ctaacccagt
     1081 ctcagaagca aaatgaagat tatgagaaga tggtaaaggc tctaagagaa acaatggaga
     1141 tcctggagac aaaccatgca gagttaatgg agcatgaggc atctctcagt aggaatgctc
     1201 aagaggaaaa gttgtctctg cagcaggtga tcaaggctat aacccaggca ctggcctcag
     1261 tggaagaaga agacactgtg acccagtcct caggccatga ggactcgctg cagtcagact
     1321 gtaatggcct ttcccagttt gatccccagg acccagacag ggctcttacc ctggtgcagt
     1381 cagtgctgac tcggagacag caggctgtgc aggacctcag gcagcagctt tcaggctgcc
     1441 aggaggctat gagcttcttg cagcagcagc acgatcagtg ggaggaagag ggcagggccc
     1501 tgagggaaaa gctacagaag ctcactgggg agcgggatgc tctggcaggc cagactgtgg
     1561 gccttcaggg agaggtggac tctctcactg gggagcggga tgctctggca ggccagactg
     1621 tggaccttca gggagaggtg gactctctca gcagggagcg ggagctgctt cagaaggcca
     1681 gaggagagct gcagcagcaa ctggaagtgc tggagcagga agcgtggcga ctgcggagga
     1741 tgaacatgga gctgcagctg cagggggact cagctcaagg ggagaagctg gagcagcagg
     1801 aggagttgca cttggctgtc cgggagaggg agcgccttca ggagacgctg gtgggcctgg
     1861 aagccaagca gtcagaatca ctaagtgagc tgctcactct tcgagaagcc ctggagtcaa
     1921 gtcgcctaga aggggagtta ctgaagcagg agcgagtgga agtggctgca gccttggcca
     1981 gggcagaaca gtccattgtg gagctgtcag gttctgagaa cagcctgaag gcagaggtag
     2041 ctgatcttcg agctgcagct gtcaagcttg gggccttaaa cgaggctttg gctttagata
     2101 aagttgaact gaaccagcag cttctccagt tggagcagga gaaccagtct ctatgcagca
     2161 gagtggaggc cgcagagcag ctgagaagcg ctttgcaggt ggacctggcg gaggcagaaa
     2221 ggcgcaggga agccctgtgg gagaagaaaa ctcagctgga gactcagctg cagaaggcag
     2281 aggaagcggg ggctgagcta caggcagagc tgaggggcac ccgagaagaa aaggaagagc
     2341 ttaaagataa attaagtgag gcacatcacc agcaagaaac agctacagct cacctagaac
     2401 agctgcatca ggacgcagaa agacaggaag aaacacttgc tagggcagtc caggagaagg
     2461 aagccctagt acgggagagg gcagctcttg aggtgcgcct gcaggctgtg gagcgggacc
     2521 gacaggacct cactgaacat gtcctggggc tcaggtcagc caaggagcaa ctggagagca
     2581 atctatttga ggcccaacag caaaattctg tgatacaggt caccaagggg cagctggagg
     2641 tccagattca aactattatc caagccaagg aagtaattca aggggaagtg aaatgcctga
     2701 agctggaact ggatgctgag aggacccggg cagagcagga gtgggatgca gtagccaggc
     2761 agctagccca agctgagcag gaggggcaag catctctgga gcgacagaag gtggcccacg
     2821 aggaggaggt gaacaggctg caagagaaat gggagaaaga gcgctcctgg ctgcagcagg
     2881 agctggataa gaccctggaa accctcgaga gggagagagc agagctggag acaaagctga
     2941 gagagcagca gacagaaatg gaagccatcc gtgcacagag ggaagaggaa cgaagccagg
     3001 ctgacagcgc cctgtatcaa atgcagctag aaacagagaa ggagagagta tccctcctgg
     3061 agacactgct gaggacccag aaggagctgg cagatgctag tcaacagctg gaaaggctga
     3121 gacaggacat gaagattcag aagttaaagg aacaggagac cactggcatg ctgcaggccc
     3181 agcttcagga aacacagcag gagctgaagg aggcagccca gcagcacagg gacgaccttg
     3241 ctgccttcca gaaggacaag ctggacctgc agaagcaggt agaggaccta atgtctcagc
     3301 tggtagccca cgatgactcc cagaggctgg tgaaagaaga gattgaagag aaggtgaaag
     3361 tggctcaaga atgcagccgg attcagaagg aactggagaa agagaacgcc agcctggctc
     3421 tgtcgctggt ggaaaaggaa aaacgacttc tcattttaca agaagctgac tctgtaagac
     3481 agcaggagct gagctccttg cgccaggaca tacaggaggc ccaggaaggg caaagagagc
     3541 tgggtgtaca ggtggaacta ttgaggcagg aggtgaagga aaaggaggct gactttgtgg
     3601 cccgggaagc acagctgctg gaggagttgg aggcctcacg ggttgcagag cagcaacttc
     3661 gagcgtcttt gtgggcccag gaagccaagg caacccagct gcagctgcag ctgcgcagca
     3721 ctgagagcca gctagaggca ttggttgcag aacaacagcc ggagaaccag gcacaggcac
     3781 agctagctag cctctgctct gttctgcagc aggccctggg gtctgcatgt gagagtaggc
     3841 ctgagctgag gggtggaggc gactctgctc ctaccttatg gggccctgat ccagatcaga
     3901 atggagcttc cagactcttt aagagatggt cgctcccaac ggcactgtca ccggaggctg
     3961 tggcactagc tctccagaag ctccatcagg acgtgtggaa ggctcggcag gcccgggatg
     4021 acctaaggga ccaggtccag aagctggtgc agcgcctgac tgacacggag gcccagaaga
     4081 gccaggttca ctcggagctg caggacctgc agagacagct ctctcagagc caggaagaga
     4141 aatccaagtg ggaagggagg cagaactccc tggagtctga gctgagggac ttgcacgaga
     4201 ccgcggcatc cttacagagt cgcctccggc aggcagaact gcaaaaaatg gaagctcaga
     4261 atgatcgaga gttgcttcag gcatccaagg agaagctgtc agcccaggtt gaacatctgc
     4321 aagcatgtgt tgcagaagcc caggctcagg cagatgcagc tgctgtcctg gaagaagatc
     4381 tgcgaactgc tcgctctgca ctgaaactga aaaacgagga gctagagagt gaacgggaga
     4441 gagcccaggc tttacaagaa cagggtgagc tgaaggtggc tcaggggaaa gctttgcagg
     4501 agaacctagc cctgctggca cagaccctgt ctaacagaga gcgggaggtc gagacgttgc
     4561 aggctgaggt tcaggaactg gagaagcagc gggagatgca gaaggctgct ctggagctgc
     4621 tctccctcga cctgaagaag cgcagtcggg aggtagacct gcaacaagaa cagattcagg
     4681 agctggagca gtgcaggtca gtcttagagc acttgcccat ggccgtccaa gagcgagagc
     4741 agaaactgag tgtgcagcgg gaccagatca gggaactgga gaacgatcgg gaggcccaga
     4801 ggagtgtcct ggaacaccag cttctggacc tggagcagaa ggctcaagtg attgagtccc
     4861 agaggggaca gattcaggat ctgaaaaagc aactggggac actggaatgc ctggccctgg
     4921 aactggagga aagccatcac aaagtggaga gccaacagaa gatgatcaca gaactagagg
     4981 gccagaggga aatgcagagg gtggctctaa cccacctcac actggacctg gaagaaagga
     5041 gccaggagct gcaggcacaa agcagccagc tccatgagct ggagaaccat agcacccatc
     5101 tggcaaaaga gctgcaggag agggaccagg aggtgacgtc ccaacgccag cagatcgatg
     5161 agctgcagaa gcagcaggaa cagctggctc aggccctgga gaggaaaggc caggagctgg
     5221 tgctgcagaa ggaaaggatt caggttctgg aagatcagag gacgctgcag accaagatct
     5281 tagaggagga cctagaacag atcaagcatt ccttgagaga acgcagccag gagctggcct
     5341 ctcagtggca gctggtgcat gagcgtgccg acgatggaaa gagcccgagt aaaggccagc
     5401 gagggagcct ggagcacttg aagctgatcc tgcgtgataa ggagaaggag gtagagtgcc
     5461 agcaggagcg catccaggaa cttcagggcc acatgggcca gctggaacag cagctgcagg
     5521 gtctgcacag gaaggtgggg gagactagcc tgctcctgac ccatcgagag caggagacag
     5581 caaccctgca gcagcatctc caggaagcca aggaacaagg ggaactgaga gagcaggtac
     5641 ttcagggtca gctggaggag gcccagagag atctggccca gagggaccat gaactagaga
     5701 ccctccggca ggaaaaacag cagacccagg accaggagga aagcatgaag ctaaagacaa
     5761 gtgctctgca ggccgctctg gagcaggccc atgcgacact gaaggagcgt cagggggagc
     5821 tggaagaaca cagggaacag gtgcgaaggc tccaggaaga actagaagtg gagggacggc
     5881 aggtacgggc cctggaggag gtgttggggg acctaagggc tgagtctcgg gagcacgaga
     5941 aggctgtgct ggcccttcaa caacggtgtg ctgagcaggc ccaggaacat gaggcagaag
     6001 ccaggaccct gcaggacagc tggctgcagg cccaggccac tctcacagaa caagaacaag
     6061 agctagcggc actgcgagcg gaaaaccagt attcccgccg acaggaagag gctgcagtta
     6121 gccaggcaga ggcgctgcag gaggccctca gcaaagccca ggctgccctg caggagaaag
     6181 agcagagtct gcttgaacag gctgagttga gccacaccct ggaggccagc actgccgcct
     6241 tgcaagctac cctggacacc tgccaggcaa gtgcccggca gctggaggag gccctgagga
     6301 ttcgggaagg tgagatccag gcccaggctc tccaacacca cgaggtcacg cagcacctcc
     6361 agcaggaact ttgccagaag aaagaagagc tgaggcagct actagagaag gctggggccc
     6421 ggaggagtca agaaaatgga atccaagaga agcaaagtct ggagcaagag agacaagagg
     6481 aaacaaggag gctccttgag agcctaaagg agctacagct gactgtagcc caaagggaag
     6541 aagagattct gatgctgagg gaggcctcgt ccccacgcca cagagccctc ccagccgaga
     6601 agccagctct gcaaccgctc ccagcacaac aggagttaga gcggctgcag actgccctaa
     6661 ggcaaacaga ggccagggag attgaatgga gggagaaggc tcaggacctg gctctgtccc
     6721 tggcccaaag caaggccagc atcagcagtc tgcaggagat aaccatgttc ctacaagcct
     6781 ctgttctaga gcgggaatca gaacaacaaa gactacagga ggagttggta ctcagcagac
     6841 aagctctgga agagcagcag tcaggtggcc cacactcaac cagcagagca gaccagggac
     6901 caaaagtggg acagggaagc cagtctggag aggtagagac tgagcctagt cctggagtgg
     6961 aggagaagga acggttgaca cagaggcttg aacgtctgca gcaagcggtg gcagagctgg
     7021 aagttgaccg aagcaagctg cagtgccaca atgcgcagct gcggaccgcc ttggagcagg
     7081 tggaacgaga acggaggaag ctgaagaggg attccgtgcg tgcatcccgg gcaggatcac
     7141 tggaggccag agaaactatg acttcatccc ccacacagca ggatgggaga ggcagtcaga
     7201 ggggttcctc ggacagcgtg cttgtggttg agcttcagag agaggtagcc ctgctgcgag
     7261 cccagctggc cttggagcga aagcagaggc aggactacat tgcacggtcc gtgcagacca
     7321 gccgtgagct agcaggcctg catcacagcc tgtctcactc cctcctcacc gtggcccagg
     7381 cccctgaggc cactgttctg gaggctgaga cccgaaagct ggatgagtct ctgaatcaga
     7441 gtctgacatc cccagggcca tgtttgctac atcccagtct ggacactact caaaataccc
     7501 acaggtagta gccataggca gggcaggaca caggcgagtc aggtgggaca cacattggat
     7561 gactgcagag aggccaaacc cccccccccc cccacgaccc agctacaagt ttgccaaagg
     7621 cgagatttac ctgttactca acctgggaga ccctagccag ctgacagccc ctggaagaaa
     7681 acgagaaccc tccaggctgg ggaaggtccc aggacacatg ggaatgaggc aaaatcacat
     7741 ttgcttgcta acctgctacc caagaagtgc cttgccctga ccgtggggca tctgttgtct
     7801 tcccgccgga tgaagcaacc acagtctaca ctaagcctga agacctgaca gcatatgagt
     7861 attctgtcct gggcaggagc atcctgaatg tatcaggtac aggagtgtcc atggcctgtt
     7921 tgtcttcagt gtctccctca tcttcccttc gacaataaat cctaggcctt tgcgtta
//
