LOCUS       NM_027973               3451 bp    mRNA    linear   ROD 08-JUN-2024
DEFINITION  Mus musculus centromere protein U (Cenpu), transcript variant 1,
            mRNA.
ACCESSION   NM_027973 XM_134129
VERSION     NM_027973.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Tian,T., Chen,L., Dou,Z., Yang,Z., Gao,X., Yuan,X., Wang,C.,
            Liu,R., Shen,Z., Gui,P., Teng,M., Meng,X., Hill,D.L., Li,L.,
            Zhang,X., Liu,X., Sun,L., Zang,J. and Yao,X.
  TITLE     Structural insights into human CCAN complex assembled onto DNA
  JOURNAL   Cell Discov 8 (1), 90 (2022)
   PUBMED   36085283
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3451)
  AUTHORS   Saito,M., Kagawa,N., Okumura,K., Munakata,H., Isogai,E.,
            Fukagawa,T. and Wakabayashi,Y.
  TITLE     CENP-50 is required for papilloma development in the two-stage skin
            carcinogenesis model
  JOURNAL   Cancer Sci 111 (8), 2850-2860 (2020)
   PUBMED   32535988
  REMARK    GeneRIF: CENP-50 is required for papilloma development in the
            two-stage skin carcinogenesis model.
REFERENCE   4  (bases 1 to 3451)
  AUTHORS   Feng,G., Zhang,T., Liu,J., Ma,X., Li,B., Yang,L., Zhang,Y., Xu,Z.,
            Qin,T., Zhou,J., Huang,G., Shi,L. and Xiao,Z.
  TITLE     MLF1IP promotes normal erythroid proliferation and is involved in
            the pathogenesis of polycythemia vera
  JOURNAL   FEBS Lett 591 (5), 760-773 (2017)
   PUBMED   28173615
  REMARK    GeneRIF: MLF1IP transgenic mice developed polycythemia vera
            (PV)-like symptoms based on the analysis of hematological features,
            suggesting that MLF1IP is partially involved in the pathogenesis of
            PV.
REFERENCE   5  (bases 1 to 3451)
  AUTHORS   Kagawa,N., Hori,T., Hoki,Y., Hosoya,O., Tsutsui,K., Saga,Y.,
            Sado,T. and Fukagawa,T.
  TITLE     The CENP-O complex requirement varies among different cell types
  JOURNAL   Chromosome Res 22 (3), 293-303 (2014)
   PUBMED   24481920
REFERENCE   6  (bases 1 to 3451)
  AUTHORS   Wang,X., Marcinkiewicz,M., Gatain,Y., Bouchard,M., Mao,J.,
            Tremblay,M., Uetani,N., Hanissian,S., Qi,S., Wu,J. and Luo,H.
  TITLE     Investigation of tissue-specific expression and functions of
            MLF1-IP during development and in the immune system
  JOURNAL   PLoS One 8 (5), e63783 (2013)
   PUBMED   23724000
  REMARK    GeneRIF: MLF1-IP expression at about 50% of its normal level is
            sufficient to sustain mice life and the development of their immune
            system without apparent abnormalities.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3451)
  AUTHORS   Hanissian,S.H., Akbar,U., Teng,B., Janjetovic,Z., Hoffmann,A.,
            Hitzler,J.K., Iscove,N., Hamre,K., Du,X., Tong,Y., Mukatira,S.,
            Robertson,J.H. and Morris,S.W.
  TITLE     cDNA cloning and characterization of a novel gene encoding the
            MLF1-interacting protein MLF1IP
  JOURNAL   Oncogene 23 (20), 3700-3707 (2004)
   PUBMED   15116101
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC119267.8.
            
            On Feb 13, 2019 this sequence version replaced NM_027973.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK050386.1, BC117785.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                AC119267.8         170002-170060       c
            60-108              AC119267.8         168066-168114       c
            109-220             AC119267.8         165936-166047       c
            221-323             AC119267.8         162158-162260       c
            324-384             AC119267.8         160291-160351       c
            385-612             AC119267.8         159484-159711       c
            613-682             AC119267.8         153450-153519       c
            683-791             AC119267.8         152050-152158       c
            792-870             AC119267.8         149299-149377       c
            871-918             AC119267.8         148649-148696       c
            919-980             AC119267.8         148219-148280       c
            981-1137            AC119267.8         145747-145903       c
            1138-3451           AC119267.8         141561-143874       c
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 26.38 cM"
     gene            1..3451
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /note="centromere protein U"
                     /db_xref="GeneID:71876"
                     /db_xref="MGI:MGI:1919126"
     exon            1..59
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..1251
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /note="isoform 1 is encoded by transcript variant 1;
                     MLF1-interacting protein; CENP-U; myeloid leukemia factor
                     1 interacting protein"
                     /codon_start=1
                     /product="centromere protein U isoform 1"
                     /protein_id="NP_082249.1"
                     /db_xref="CCDS:CCDS22292.1"
                     /db_xref="GeneID:71876"
                     /db_xref="MGI:MGI:1919126"
                     /translation="MAARRSLRYSGNPGAKHSKNTLRSTYSRKQKAGPKPRPKDVFDF
                     SNNSDASSIPGALEEEEETYETFDPPLHSTAIYAEDELSKHCVSSSSLATHRGKASRN
                     LDPSEDEASGNESIKVSTKKPRRKLEPISGESDSSADDVRRRVASAEGPRSQQRQAAP
                     AAPSPPERPAEPVTPRRTRLHSAQLSPVDETPATQSQLKTQKKVRPSPGRRKRPRRGH
                     TDTDGSESMHIWCLEGKRQSDITELDVILSVFEKTFLEYKQRVESESCNQAINKFYFK
                     MKGELIRMLKEAQMLKALKMKNTKIIANMEKKRQRLIEVQDELIRLEPQLKQLQTKYD
                     DLKERKSSLKKSKHFLSNLKQLCQDYSNVQEKGPKGTGKYDSSSLPALLFKARSILGA
                     ENHLRTINYQLGKLLELD"
     exon            60..108
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     exon            109..220
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     exon            221..323
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     exon            324..384
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     exon            385..612
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     exon            613..682
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     exon            683..791
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     exon            792..870
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     exon            871..918
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     exon            919..980
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1137
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..3451
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1491..1496
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /note="hexamer: AATAAA"
     polyA_site      1522
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /note="major polyA site"
     regulatory      1582..1587
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /note="hexamer: AATAAA"
     polyA_site      1606
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
     regulatory      1623..1628
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /note="hexamer: TATAAA"
     polyA_site      1648
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
     regulatory      2575..2580
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /note="hexamer: AATAAA"
     polyA_site      2598
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
     regulatory      2871..2876
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /note="hexamer: ATTAAA"
     polyA_site      2895
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
     regulatory      3191..3196
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
                     /note="hexamer: ATTAAA"
     polyA_site      3214
                     /gene="Cenpu"
                     /gene_synonym="1700029A22Rik; Mlf1ip"
ORIGIN      
        1 gcctttggga acggtaggat ggctgcaagg agaagtttga gatactcggg gaacccgggt
       61 gcaaaacatt caaagaacac tttgagaagc acatattcca ggaaacagaa agctggtcca
      121 aagccccggc ccaaggatgt gtttgacttt tctaataatt ctgatgcctc aagcataccg
      181 ggagcactcg aggaagaaga ggagacttac gaaaccttcg acccccctct gcacagtacg
      241 gctatctacg cagaagacga gctgtccaaa cactgtgtgt cctccagttc cctggccacc
      301 cacagaggga aggcgagcag aaacttggac ccttctgaag atgaagcaag tggaaatgag
      361 tctatcaagg tcagcacaaa aaagccaagg agaaagctgg agcccatcag cggcgagtcg
      421 gacagctctg cagatgatgt aaggaggagg gtggcatctg cagaaggacc aaggtcacag
      481 caacgccagg ctgctcctgc agcaccgtca ccccctgaga ggccagccga gccggtcact
      541 cccaggagga caagactcca cagcgctcag ctctctcctg tagacgagac accagcaacc
      601 caaagtcagc tgaaaactca gaagaaagtg cggccgtctc ctggcaggag gaagagacca
      661 aggcgcggac acacagacac agatggttct gaaagtatgc atatctggtg tctggaagga
      721 aagagacaga gtgacataac ggagttggat gttattctgt ctgtatttga gaaaaccttc
      781 ctggagtata agcaaagagt agaatctgaa agttgtaatc aagccatcaa caagttttat
      841 tttaaaatga aaggagaact catcagaatg cttaaagaag cccagatgtt aaaagccctg
      901 aaaatgaaga acaccaagat aattgccaac atggaaaaga aaaggcagcg tttaattgaa
      961 gtccaggatg aactgattcg attagaacca caactgaaac aactacaaac aaaatatgat
     1021 gaccttaagg agagaaagtc atcacttaag aagtccaagc atttcttatc taatttaaaa
     1081 cagctgtgtc aagattattc aaacgttcaa gagaaaggac caaagggaac gggaaagtat
     1141 gattcatcta gccttccagc tctgttattt aaagcaagaa gcattctggg cgccgaaaac
     1201 cacctgagaa ccatcaacta tcagctgggg aagcttctcg agctggactg agaactgagc
     1261 ctcctgctgc cttttctgct cctgaagcca tgctcctgac agcagcggcc tgtggtgaca
     1321 gtagtgagac ctgcttatca gggtgtacta ccgttttgtc attcaaacta aattacacca
     1381 attcagttgt ctaataactc agaaggcata gccatatttt tgagtattgt gttaataaat
     1441 ttcagtctct tgacaggtta gtcatcacca ggcataagct ttttaactac aataaacttt
     1501 aatgatgtca gtgcaaatgc aagcatagaa tgatcctggt taattaggag cgtctgctga
     1561 aatatgtcaa aggtagtctt caataaatct ggcttaggta aaccaagtga tagaacggtt
     1621 gctataaaca ggttttacaa taagttaata agttgatctt ttgtatgatg ttaaatgcag
     1681 ggccttgcac atcttaggta agtattctaa tgttgggcta cagtcccggc actaatttaa
     1741 catttatgtt atcaaatctc aggcttcagg caaattgtag cctggttatg tatgttctgt
     1801 ccacagtcag ctattctgta aagcttctat aattagttca tcaggaatat catccattga
     1861 accaaggctc ttatctgcag cgtcagcaaa ttcagcatct tctgtcgctt ctaagaaaag
     1921 ggcgtcatct tcgtcatccc aggcagcggc agaaagcccg ccggctgaca agttgcagga
     1981 tggcggggtt tgagaatcct tggtcagtga ggtgctggtg tcatctgttt ctccagaagt
     2041 gaagtcttcc tttattttgg agggaagagg aataaagatt agtcagaata tgtgacttca
     2101 aatacgttga gttaaggtat gaaataataa ccaggttttc agcagtcagt gaccatgctg
     2161 gctgcctgct gtcaggaaca gtcagcatgg ggaaggctaa gttagctcag aagtgctttt
     2221 gtagcgcaga catgcttctc tgaggctgaa actgactgta gctcatggca gatacatttc
     2281 aactcctcag aaggggtaca gcaaagggaa gtttctagat taacaaaaaa tcaatgccta
     2341 gaatctatgt taaaaagcca gcatgcaatc ccagtgctag ggaggtggag acatggctcc
     2401 ctggagcttg gtagccatcc agccaagcct cccaactgaa caccagccca gtgagaggct
     2461 gcatctcaaa aaagaaaaaa aaaatgtcct gaggaatgac acccaaagtt gtccctctgg
     2521 cctgtgtgcg tgtgtacatg agcacacatg tgagcatgga cacacaaatg tataaataaa
     2581 aggaagatga aggagaaatg gcagtgttaa cagttcactt ggaaggtact atttctatac
     2641 tcgtgtttct agaagaaaaa catgttcaga taatagtaat attgtatatc aagaatactt
     2701 tcacttggca tttttgaggt attattagaa aagaaaaaat ttctaccaaa gatgtcgcca
     2761 cagtaaatta atggctggga atgtagctca agagagagcc cttgcttccc atgtatgtgg
     2821 ctgataagtt caattctatc caaaaccaaa cactgctctt ataagagata attaaacatt
     2881 tttaatctgt tcctagatac tattttaaat gtagtagtat gtcagagtct gcttatcagt
     2941 tgttatgtcc cgctctaaca taccaagcat gggattttaa ctttaagctt ggatgttggg
     3001 tctggagaga tggctaagag cattgctgct cttccagagg acccctccag ggctgcagtt
     3061 cccaggacca catggtggct cacaaccacc accaactaca gttccaggga acttctgctt
     3121 ccttgggtac caaacataca tgtggtatgc acagatgtac atgcaatcaa atcactcata
     3181 catatataaa attaaaaaac caacttgaat atgatgtttt cagaagagat ttttaagtac
     3241 agatctcaaa ggatgccagg cggtttctag actgtagtgg aaggagcact tgttcggcct
     3301 gtaacaggtc aggtacccca tcatggcagt ttgaaagact gtgctgatgg ttttacagtg
     3361 aaaatgaaaa taaaaaatta actctaaata acacatgaca aaaagtatta aaaattatca
     3421 gaaaaattga tccagaaatt cataaaataa c
//
