LOCUS       NM_173762               7813 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus centromere protein E (Cenpe), mRNA.
ACCESSION   NM_173762
VERSION     NM_173762.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   He,S., Gillies,J.P., Zang,J.L., Cordoba-Beldad,C.M., Yamamoto,I.,
            Fujiwara,Y., Grantham,J., DeSantis,M.E. and Shibuya,H.
  TITLE     Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate
            mitotic and male meiotic spindle bipolarity
  JOURNAL   Nat Commun 14 (1), 1715 (2023)
   PUBMED   36973253
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7813)
  AUTHORS   She,Z.Y., Xu,M.F., Jiang,S.Y. and Wei,Y.L.
  TITLE     Kinesin-7 CENP-E is essential for chromosome alignment and spindle
            assembly of mouse spermatocytes
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1869 (9), 119306 (2022)
   PUBMED   35680098
  REMARK    GeneRIF: Kinesin-7 CENP-E is essential for chromosome alignment and
            spindle assembly of mouse spermatocytes.
REFERENCE   4  (bases 1 to 7813)
  AUTHORS   Xu,M.F., Yang,Y.H., Wei,Y.L., Zhang,J.L., Lin,X., Lin,X.Y., Chen,H.
            and She,Z.Y.
  TITLE     Functional Assessment of Kinesin-7 CENP-E in Spermatocytes using In
            Vivo Inhibition, Immunofluorescence and Flow Cytometry
  JOURNAL   J Vis Exp (2021)
   PUBMED   35037651
  REMARK    GeneRIF: Functional Assessment of Kinesin-7 CENP-E in Spermatocytes
            using In Vivo Inhibition, Immunofluorescence and Flow Cytometry.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7813)
  AUTHORS   He,H., Chen,J., Zhao,J., Zhang,P., Qiao,Y., Wan,H., Wang,J.,
            Mei,M., Bao,S. and Li,Q.
  TITLE     PRMT7 targets of Foxm1 controls alveolar myofibroblast
            proliferation and differentiation during alveologenesis
  JOURNAL   Cell Death Dis 12 (9), 841 (2021)
   PUBMED   34497269
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7813)
  AUTHORS   Uren,A.G., Wong,L., Pakusch,M., Fowler,K.J., Burrows,F.J.,
            Vaux,D.L. and Choo,K.H.
  TITLE     Survivin and the inner centromere protein INCENP show similar
            cell-cycle localization and gene knockout phenotype
  JOURNAL   Curr Biol 10 (21), 1319-1328 (2000)
   PUBMED   11084331
REFERENCE   7  (bases 1 to 7813)
  AUTHORS   Craig,J.M., Earnshaw,W.C. and Vagnarelli,P.
  TITLE     Mammalian centromeres: DNA sequence, protein composition, and role
            in cell cycle progression
  JOURNAL   Exp Cell Res 246 (2), 249-262 (1999)
   PUBMED   9925740
  REMARK    Review article
REFERENCE   8  (bases 1 to 7813)
  AUTHORS   Fowler,K.J., Saffery,R., Kile,B.T., Irvine,D.V., Hudson,D.F.,
            Trowell,H.E. and Choo,K.H.
  TITLE     Genetic mapping of mouse centromere protein (Incenp and Cenpe)
            genes
  JOURNAL   Cytogenet Cell Genet 82 (1-2), 67-70 (1998)
   PUBMED   9763662
REFERENCE   9  (bases 1 to 7813)
  AUTHORS   Nakagawa,T., Tanaka,Y., Matsuoka,E., Kondo,S., Okada,Y., Noda,Y.,
            Kanai,Y. and Hirokawa,N.
  TITLE     Identification and classification of 16 new kinesin superfamily
            (KIF) proteins in mouse genome
  JOURNAL   Proc Natl Acad Sci U S A 94 (18), 9654-9659 (1997)
   PUBMED   9275178
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1999 Mar 30;96(7):4214]
REFERENCE   10 (bases 1 to 7813)
  AUTHORS   Winter,H., Siry,P., Tobiasch,E. and Schweizer,J.
  TITLE     Sequence and expression of murine type I hair keratins mHa2 and
            mHa3
  JOURNAL   Exp Cell Res 212 (2), 190-200 (1994)
   PUBMED   7514534
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK133445.1, AY493378.1, CN534554.1, CN704540.1 and AC104874.11.
            
            On Oct 7, 2006 this sequence version replaced NM_173762.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164134,
                              SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-96                AK133445.1         2-97
            97-3764             AY493378.1         1-3668
            3765-4055           CN534554.1         390-680
            4056-4925           AY493378.1         3969-4838
            4926-5052           CN704540.1         350-476
            5053-7512           AY493378.1         4966-7425
            7513-7813           AC104874.11        107510-107810
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 62.55 cM"
     gene            1..7813
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /note="centromere protein E"
                     /db_xref="GeneID:229841"
                     /db_xref="MGI:MGI:1098230"
     exon            1..152
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..7512
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /note="centromeric protein E; motor domain of KIF10;
                     kinesin superfamily protein 10; kinesin 10; N-7 kinesin;
                     centromere autoantigen E"
                     /codon_start=1
                     /product="centromere-associated protein E"
                     /protein_id="NP_776123.3"
                     /db_xref="CCDS:CCDS51072.1"
                     /db_xref="GeneID:229841"
                     /db_xref="MGI:MGI:1098230"
                     /translation="MAEEASVAVCVRVRPLNSREEELGEATHIYWKTDKNAIYQSDGG
                     KSFQFDRVFDSNETTKNVYEEIAVPIISSAIQGYNGTIFAYGQTASGKTHTMMGSEDC
                     LGVIPRAIHDIFQRIKKFPEREFLLRVSYMEIYNETITDLLCNAQKMKPLIIREDTNR
                     TVYVSDLTEEVVYTAEMALKWLATGEKNRHYGITKMNQRSSRSHTIFRMILESREKAE
                     PSNCDGSVKVSHLNLVDLAGSERAAQTGAEGVRLKEGCFINRNLFILGQVIKKLSDGQ
                     VGGFINYRDSKLTRILQNSLGGNAKTRIICTITPASLDETLTTLQFASTAKYMKNTPY
                     VNEVSNDEALLKRYRREIADLRKQLEEVNTKTRAQEMEKDQLAQLLDEKDLLQKVQDE
                     KINNLKRMLVTSSSIALQQELEIKRKRRVTWCYGKMKDSNYEKEFKVPTSITTRKRKT
                     SVTSLRENSLMKFGESAASSEFEMLNNTLESLAEVEWSSATTLLSEENVESELNSLNA
                     QYNDLVLDYEQLRRENEDLKLKLKEKNELEEFELLEQKEERDQEMQLMHEVSNLKNLI
                     KHAEEYNQDLENDLSSKVKLLKEKEEQIKNLQEYIDAQKSEKMKIDLSYTSDATEDLK
                     QAMRTLSDLDTVALDAKKESAFLRSENLELKEKINELSDSRKQMESDIQMYQRQLEAK
                     KKMQTDLDKELQLAFQEISKLSALVDGKGLLSNLELEKRITDLQKELNKEAEEKQTLQ
                     EEVNLLSELKSLPSEVETLRRELYEKSEELHIITTEREKLFSEMAHKDSRIQGLLEEI
                     GNTRDDLATSQLSRRGSDGEWQALESLHAELEHRHAGVLEERERLKQEIGALSKEAES
                     LAFSLDSVKAELSHKTQELEQKTVEGQERLNKMEALREELESRDSSLQSVEKEKVLLT
                     EKLQQALKEVKALTQEKKNLKQLQESLQTERDQLRSDIQDTVNMNIDTQEQLLNALES
                     LKQHQETINMLKMKAAEELSDNLHVKDRGGARDEAQQKMDGIDEQNESAHTLLGGGKD
                     NEVTEEQRKIDSLMQENSGLQQTLESVRAEKEQLKMDLKENIEMSIENQEELRILRDE
                     LKRQQEVAAQEKDHATEKTQELSRTQERLAKTEEKLEEKNQKLQETQQQLLSTQEAMS
                     KLQAKVIDMESLQNEFRNQGLALERVETEKLELAQRLHESYEEVKSITKERNDLKELQ
                     ESFEIEKKQLKEYAREIEAAGLQTKEELNIAHANLKEYQEIITELRGSISENEAQGAS
                     TQDTAKSAPELQGEVPEQELLPVVKEARHSAEKVNGLEPVGAHSRTVHSMTMEGIEIE
                     NLRLTKKLEESQMEISCLTREREDLRRTQETLQVECTQLKEDARRTLANHLETEEELN
                     LARCCLKEQENKIDTLITSLSQRETELSSVRGQLALTTAELERKVQELCEKQEELTRK
                     ETSEAQGKMSELEQLRELLLAQASALQNAESDRLRLNTQLEESQEEMKTLREEREELR
                     RMQEALHVESEQQKESMKEISSKLQELQNKEYECLAMKTINETQGSRCEMDHLNQQLE
                     AQKSTLEKVEMENVNLTQRLHETLEEMRSVAKERDELWSMEERLTVERDQLKKSLEET
                     VTKGMEKEEELRVAHVHLEEHQETINKLRKMVSDYTDEISHTQGDLKHTNAVVEAQNQ
                     DLREKEHQLSQVKADLRETVDQMEQLKKKLEAQSSTLESREIEKLELTQQLNENLKKI
                     TLVTKENDSLKIMDEALREERDQLRKSLQQTEARDLENQEKLRIAHMNLKEHQETIDR
                     LMETMSEKTEEISNMKMELENVNMKLQEKVQELKTSERQRVKLKADASEAKKELKEQG
                     LTLSKIEMENLNLAQKIHENLEEMKSVRKERDDLKKLEEILRMERDQLKDNLREAMLK
                     AHQNHEETMKCGKGLLCAGEYCTGRLREKCFRIEKLLKRYSEMANDYECLNKVSLDLE
                     RETKTQKELSVTVRTKLSLPHTQTKEMEKLLTANQRCSLEFHRALKRLKYVLSSIARI
                     KEEQHESINKREMAFIQEVEKQNELQIQIQSLSQTYRIPARDLQIKLSQEMDLHIEEM
                     LKDFSENDFLTIKTEVQQVLNNRKEITEFLGKWLNTLFDTENLKSTIQKENKSIGLVN
                     NFYHSRITAMINESTEFEERSATRSKDLDQYLKSLKETTEQLSEVYQTLTASQSVVHL
                     HPTVQPSTRDSERPQAASGAEQLTSKNKIALGAVPYKEEIEDLKMQLVKSDLEKKATA
                     KEFDKKILSLKATVEHQEEMIRLLRENLRGHQQAQDTSMISEQDSQLLSKPLTCGGGS
                     GIVQSTKALILKSEYKRMGSEISKLKQQNEQLRKQNNQLLSDNSQLSNEVKTWEERTL
                     KRDSYRETTCENSPKSPKVTRTDSKRRQNTTSQCRAQNLQDPVPKDSPKSWFFDNRSK
                     SLPAPHPIRYFDNSSLGLCPEPDDVENVEPKTDLCQASLEKDVSQCKTQ"
     exon            153..244
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            245..334
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            335..453
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            454..573
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            574..655
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            656..723
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            724..789
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            790..841
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            842..929
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1059
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1179
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1338
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..1495
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1584
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..1752
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1827
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..1947
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2070
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..2227
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            2228..2382
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            2383..2697
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2970
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            2971..3126
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            3127..3318
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            3319..3477
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            3478..3768
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            3769..3924
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            3925..4203
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            4204..4362
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            4363..4647
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            4648..4935
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            4936..5094
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            5095..5376
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            5377..5535
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            5536..5796
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            5797..5898
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            5899..6117
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            6118..6312
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            6313..6516
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            6517..6744
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            6745..6857
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            6858..6955
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            6956..7136
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            7137..7423
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     exon            7424..7813
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7777..7782
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /note="hexamer: AATAAA"
     polyA_site      7797
                     /gene="Cenpe"
                     /gene_synonym="312kDa; C530022J18; CENP-E; Kif10"
                     /note="major polyA site"
ORIGIN      
        1 aaccttgagg acattttaaa cttaaaggcg gggcggcccg ggagctcggt cggcgccgtt
       61 gctctccttc gtttccctta gtctgcagcg gacaggatgg ccgaggaggc ttcagtggct
      121 gtctgtgttc gtgtgcgacc cctcaacagc agagaagaag aacttggaga agccacccac
      181 atttactgga aaacagataa aaacgctatt tatcagagcg atgggggtaa atctttccaa
      241 tttgatcgtg tctttgacag taatgaaacc acgaagaatg tgtatgaaga gattgcggtg
      301 cccatcatca gctccgccat acaaggctac aatggtacta tatttgccta tggacaaact
      361 gcatcaggaa agacacacac gatgatgggt tctgaagatt gtttgggagt tatacccagg
      421 gcaattcatg acattttcca aaggataaag aagtttcctg agagagaatt tctcctacga
      481 gtttcttaca tggagatcta caatgaaacc attacagatt tactctgtaa tgctcaaaaa
      541 atgaaacctt tgataattcg ggaagacacc aataggactg tatatgtttc tgatctcaca
      601 gaagaagtgg tttatacagc agaaatggct ttgaaatggc tcgcaacagg agaaaaaaac
      661 agacattatg gaataaccaa aatgaatcaa agaagcagtc gttctcatac gatttttagg
      721 atgattctgg aaagtagaga aaaggctgag ccttctaact gcgatggctc cgtcaaagtg
      781 tctcatctga atttggtcga tcttgcaggc agtgaaagag ctgctcaaac aggagctgaa
      841 ggtgtgcgat tgaaggaggg ctgttttata aatcggaact tgtttatttt gggacaagtg
      901 attaagaaac tcagtgatgg acaggttggg ggtttcataa attaccgaga tagcaaattg
      961 acacgaattc ttcagaattc cttgggagga aatgccaaga cgcgcatcat ctgcacgatc
     1021 accccagcgt ccctggacga aaccctgacg acgctgcagt ttgccagcac tgctaagtat
     1081 atgaagaata ctccttacgt taatgaagtg tcgaatgatg aagctctctt gaaaagatac
     1141 agaagagaaa tagcggatct tagaaaacaa ttagaggagg taaatacaaa gactcgggca
     1201 caggaaatgg aaaaggatca actggcccag cttctggatg aaaaagactt gcttcagaaa
     1261 gttcaggatg agaaaattaa taacctgaag cggatgctgg tgacgtcatc ttctattgca
     1321 ttgcagcagg agctagagat taaaaggaaa cgaagagtta cttggtgcta tggcaaaatg
     1381 aaggattcaa actatgaaaa ggaatttaaa gtcccgacaa gcataacaac aagaaaacgt
     1441 aaaacttctg tgacttcttt gagagaaaat tctctcatga agttcggaga gagtgcagcc
     1501 tcttccgagt ttgaaatgtt gaataacact cttgagtcat tggctgaggt agagtggagc
     1561 tcggcaacaa cgctcctaag cgaggaaaat gtagaaagtg agttgaactc acttaatgct
     1621 caatataatg atctggtatt agactatgaa cagctcagaa gagaaaatga agacttgaaa
     1681 ttgaaattaa aagaaaaaaa tgagttagag gaatttgagc ttctagaaca aaaagaagaa
     1741 agagatcaag agatgcaact aatgcatgaa gtttccaact taaagaattt aattaaacac
     1801 gcagaagaat ataatcaaga ccttgagaat gacctaagtt caaaagtgaa gcttcttaaa
     1861 gaaaaagagg agcagattaa gaacctgcag gaatacatag atgcccagaa gtcagaaaag
     1921 atgaagatag acctgtcata cacatcggac gccactgaag acctgaaaca ggcgatgcgc
     1981 accctgtctg atttggacac agtggccctt gacgctaaga aagagtcagc cttccttaga
     2041 agtgaaaatc tggagctgaa ggagaaaatt aatgaacttt cggattcccg taagcaaatg
     2101 gaaagtgaca ttcagatgta tcaacgccaa ctggaagcaa aaaagaaaat gcaaactgat
     2161 ctggataagg aattacagtt ggcttttcaa gaaatatcaa aactcagcgc cctggtagat
     2221 ggcaaaggtt tactgtctaa tttggaattg gagaaaagga ttactgatct ccaaaaagaa
     2281 ctaaataaag aagctgaaga aaaacaaact ttgcaagaag aagttaactt gctgtcggag
     2341 ttgaaatctt taccgtctga ggtggaaacg ctgaggagag agctgtatga gaaatctgaa
     2401 gagctccata taataaccac agaaagagag aaactgtttt ctgaaatggc tcataaagac
     2461 agtaggattc aaggtttact tgaggaaatt ggaaacacta gagatgacct tgccacttcc
     2521 cagctgagtc ggaggggcag tgatggagaa tggcaagccc tggaaagcct gcacgcagag
     2581 ctggagcaca gacacgcggg ggtcctggag gagagagaga ggctgaagca ggagattggg
     2641 gctctctcca aagaagcaga aagcctggct ttcagtttgg attccgtgaa ggccgagctt
     2701 tctcacaaga ctcaagagct tgagcagaaa acagttgagg gtcaagaaag gctaaataaa
     2761 atggaggcgc tgagagaaga gttagaaagc agagactcca gcctgcagtc tgtagagaag
     2821 gagaaagtcc tgctcactga gaagctgcag caagccttga aggaagtaaa agccttaacc
     2881 caagaaaaga aaaacctgaa gcagctccag gagagcctgc aaactgagag agaccaactc
     2941 agaagtgaca ttcaggacac tgtgaacatg aacatagata ctcaagagca gttactaaat
     3001 gctctggagt ctctgaaaca acaccaagaa acaattaaca tgctgaaaat gaaagctgct
     3061 gaggaactat ccgataattt gcatgtaaag gacaggggag gagctagaga tgaagctcag
     3121 caaaagatgg atggcataga tgaacagaat gagagtgccc acacattgct tggtggtggt
     3181 aaggacaatg aggtaactga ggaacagagg aaaatcgact ctctaatgca ggagaatagc
     3241 ggactccaac agacactgga aagtgttaga gcagaaaagg aacaactgaa gatggaccta
     3301 aaggaaaata tcgaaatgag tattgaaaac caggaagagt taagaattct tagagatgaa
     3361 ctgaaaagac aacaagaggt agctgcacag gaaaaggatc atgccaccga gaagacccag
     3421 gagctctcca ggacgcagga aagattggca aaaacagaag agaagttaga ggaaaagaac
     3481 cagaaactcc aagagacaca gcagcaactt cttagtacac aagaagctat gagtaagctg
     3541 caggcaaagg tgattgacat ggaaagctta cagaatgaat tcaggaacca aggattggca
     3601 ttggagcgtg tggagacaga gaaactggag ttagctcaga gacttcatga aagttatgag
     3661 gaggtgaaat ctataaccaa agaaagaaat gatctaaagg aattgcagga gtcatttgaa
     3721 atagagaaga agcaacttaa agaatatgca agggaaatag aagccgcggg cctacaaaca
     3781 aaagaagaac taaacattgc ccacgcgaat ctaaaagagt accaggaaat catcacagag
     3841 ctaagaggaa gcatttctga aaacgaagca caaggagcaa gcacccagga cacggcaaaa
     3901 agcgcccctg agctacaagg agaggttcca gagcaagagc tgcttcctgt tgtcaaagaa
     3961 gccaggcaca gtgcagaaaa agtgaacgga ctggagccag tgggagcaca ttccagaaca
     4021 gtgcactcca tgacaatgga gggcatagaa atagaaaacc tcaggctgac caagaaactt
     4081 gaagaaagtc agatggagat cagctgtctc actagagaga gagaagacct gagaaggaca
     4141 caggaaacac ttcaggtcga atgtacccag ctgaaagaag atgccagaag aactttggct
     4201 aatcatctag aaactgaaga ggaacttaac cttgctcgtt gttgcctgaa agagcaggag
     4261 aacaagattg acaccctaat aacgagcctc tcgcagaggg aaactgagct gtccagtgtg
     4321 cgaggacaac tagcgctgac tactgctgaa ctggagagaa aggttcaaga actctgtgag
     4381 aaacaggaag aacttactag gaaagaaact agcgaggctc aagggaagat gagtgaacta
     4441 gagcaactca gggagctgct gcttgcccag gcctcggcac tgcagaacgc agagagtgac
     4501 aggctgcggc tgaatacaca actggaagaa agtcaagagg aaatgaaaac tctaagggag
     4561 gaaagagaag agctgagaag aatgcaagag gctcttcacg tggaaagtga gcagcagaaa
     4621 gaaagcatga aggaaatcag ctcaaaactc caagaactac agaacaaaga gtatgaatgt
     4681 cttgcaatga agactattaa tgagactcaa ggaagcagat gtgaaatgga ccacttgaac
     4741 cagcagctgg aggcccagaa gtcaactctg gaaaaggtgg agatggagaa tgtaaacttg
     4801 actcaaagac ttcatgagac ccttgaggaa atgagatctg tagcaaaaga aagagatgag
     4861 ctttggagca tggaggagcg cctcacagta gagagagacc aactgaagaa gagccttgaa
     4921 gaaacagtaa ctaaaggtat ggaaaaagag gaagagctaa gagttgcgca tgtgcatctg
     4981 gaagagcacc aggaaactat caacaagctc agaaagatgg tgtctgacta cacagatgaa
     5041 atatcacata ctcaagggga cctaaagcac acaaatgccg ttgtagaagc tcagaaccaa
     5101 gatctccggg aaaaagaaca tcaactttct caagtaaaag ctgatctcag agaaaccgtg
     5161 gatcaaatgg agcaactgaa gaagaagctc gaagcccaga gttcgactct agaaagcaga
     5221 gaaatagaga agttagaatt gactcagcaa cttaacgaaa accttaaaaa aataacactt
     5281 gtcactaagg aaaatgatag cctaaaaatt atggatgaag ccctcaggga agaacgagac
     5341 cagctacgga aaagtcttca gcaaacagag gcaagggatc tggaaaatca agagaaacta
     5401 agaattgctc acatgaatct aaaagagcac caagaaacta ttgaccgact catggagact
     5461 atgtctgaga agacagagga aatatcaaat atgaaaatgg agttagaaaa tgtaaacatg
     5521 aaattacaag aaaaggttca agaacttaag acaagtgagc gtcaacgtgt taagttaaaa
     5581 gcagatgcca gtgaagccaa gaaggagctc aaggagcagg gcttaactct gagtaagata
     5641 gagatggaga acttaaatct cgctcagaag attcatgaga accttgaaga aatgaaatct
     5701 gtaagaaaag aaagagatga tctaaagaaa cttgaggaga tactcagaat ggagagagac
     5761 cagctcaagg acaacctgag agaagccatg cttaaggccc accagaacca tgaagagaca
     5821 atgaaatgtg gaaaggggtt actgtgtgct ggagaatact gtacaggaag acttcgagaa
     5881 aagtgcttca ggatagaaaa actcctgaaa agatactcag aaatggctaa tgattatgag
     5941 tgcttgaata aagtctctct tgacttggag agagaaacta aaacccaaaa agagctgtca
     6001 gttactgtaa gaacaaagct ctcacttccg cacacacaga ccaaagagat ggaaaagctt
     6061 ctgactgcaa accagagatg ttccctggag ttccacagag ccctcaagag acttaagtac
     6121 gtgttaagct ccattgcgag gataaaggaa gagcagcatg agtccatcaa taaacgtgaa
     6181 atggctttta ttcaggaagt ggaaaagcag aatgaactgc agattcaaat ccagagcctt
     6241 tcacagactt accgtattcc agccagagac ttacagatca aactgagcca ggagatggac
     6301 ctccatattg aggaaatgct caaagatttt tcagaaaatg atttcctcac cataaaaact
     6361 gaagtccaac aagtattaaa taataggaaa gaaataactg agtttttggg aaaatggcta
     6421 aatactcttt ttgatacaga aaatcttaag agcaccatcc agaaagaaaa caaaagcatt
     6481 gggctcgtga ataacttcta tcacagcaga atcactgcca tgataaatga atcaacggag
     6541 tttgaggaaa gaagtgctac cagatccaaa gacttggatc agtacctgaa gtcactgaaa
     6601 gaaacaactg agcaactgtc tgaagtctac cagacactga ccgcctccca gtctgttgtg
     6661 catctccatc ctaccgtgca gccttccaca cgggacagtg agcgtcctca ggctgcatca
     6721 ggagctgaac aactgacctc aaagaacaaa attgctttag gagctgtgcc ctataaagaa
     6781 gaaattgagg atctcaaaat gcagctggtg aagagtgacc tagagaagaa ggcgaccgcc
     6841 aaggaattcg ataagaaaat tctctctcta aaagccactg ttgaacatca agaagaaatg
     6901 ataaggctac tgagagaaaa tcttagagga catcaacagg cccaggatac ctcaatgatc
     6961 tcagaacaag actctcagct tctgagtaaa cctctaacct gtggaggtgg cagtggaatt
     7021 gtacaaagca caaaagctct tattttgaaa agtgaatata aaaggatggg aagtgagatt
     7081 tctaagttaa agcagcaaaa tgagcagcta agaaagcaaa ataatcaact attaagtgat
     7141 aactctcaac tctccaatga ggttaaaacc tgggaggaaa gaactcttaa aagagacagt
     7201 tacagagaaa caacttgtga gaattctcca aagtctccta aagtgactcg gacagattct
     7261 aaaaggagac agaacacaac ttctcagtgc agggcacaaa atctccagga tcctgtgcca
     7321 aaagattccc caaaatcatg gttttttgat aaccggtcaa agtctcttcc agcacctcac
     7381 ccgatccgct attttgacaa ctccagtcta gggctgtgcc cagaaccaga tgacgtagag
     7441 aatgtggagc ctaagacaga cctctgccaa gcctccttgg aaaaagatgt gtcccagtgc
     7501 aagacgcagt agatgcccct gtcccgtcag agcagagcgc ccttggatgg atgcccccgt
     7561 cagccgagcg ctcttggatg cctttcctcg tgtgtccaca ctgacaaaga tgatgcaatc
     7621 caacttagtt tcagtctaat ttccacttta gaagatggag gaacaagaaa cggattgatt
     7681 gcagtgactt agaacgctgg tggcagttct ttctgcttgc atctgtgaca ttccacacgc
     7741 tgaactgtta attatttatt tttatacatt gcgaagaata aaggtcttga aggaaaattt
     7801 taatctttcc aga
//
