LOCUS       NM_007919                923 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus chymotrypsin-like elastase family, member 2A (Cela2a),
            mRNA.
ACCESSION   NM_007919
VERSION     NM_007919.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Cobo,I., Paliwal,S., Bodas,C., Felipe,I., Melia-Aloma,J.,
            Torres,A., Martinez-Villarreal,J., Malumbres,M., Garcia,F.,
            Millan,I., Del Pozo,N., Park,J.C., MacDonald,R.J., Munoz,J.,
            Mendez,R. and Real,F.X.
  TITLE     NFIC regulates ribosomal biology and ER stress in pancreatic acinar
            cells and restrains PDAC initiation
  JOURNAL   Nat Commun 14 (1), 3761 (2023)
   PUBMED   37353485
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  AUTHORS   Becari,C., Durand,M.T., Guimaraes,A.O., Lataro,R.M., Prado,C.M., de
            Oliveira,M., Candido,S.C., Pais,P., Ribeiro,M.S., Bader,M.,
            Pesquero,J.B., Salgado,M.C. and Salgado,H.C.
  TITLE     Elastase-2, a Tissue Alternative Pathway for Angiotensin II
            Generation, Plays a Role in Circulatory Sympathovagal Balance in
            Mice
  JOURNAL   Front Physiol 8, 170 (2017)
   PUBMED   28386233
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 923)
  AUTHORS   Naba,A., Clauser,K.R., Mani,D.R., Carr,S.A. and Hynes,R.O.
  TITLE     Quantitative proteomic profiling of the extracellular matrix of
            pancreatic islets during the angiogenic switch and insulinoma
            progression
  JOURNAL   Sci Rep 7, 40495 (2017)
   PUBMED   28071719
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 923)
  AUTHORS   Hale,M.A., Swift,G.H., Hoang,C.Q., Deering,T.G., Masui,T.,
            Lee,Y.K., Xue,J. and MacDonald,R.J.
  TITLE     The nuclear hormone receptor family member NR5A2 controls aspects
            of multipotent progenitor cell formation and acinar differentiation
            during pancreatic organogenesis
  JOURNAL   Development 141 (16), 3123-3133 (2014)
   PUBMED   25063451
REFERENCE   5  (bases 1 to 923)
  AUTHORS   Liu,S., Young,S.M. and Varisco,B.M.
  TITLE     Dynamic expression of chymotrypsin-like elastase 1 over the course
            of murine lung development
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 306 (12), L1104-L1116 (2014)
   PUBMED   24793170
REFERENCE   6  (bases 1 to 923)
  AUTHORS   Bonnart,C., Deraison,C., Lacroix,M., Uchida,Y., Besson,C.,
            Robin,A., Briot,A., Gonthier,M., Lamant,L., Dubus,P., Monsarrat,B.
            and Hovnanian,A.
  TITLE     Elastase 2 is expressed in human and mouse epidermis and impairs
            skin barrier function in Netherton syndrome through filaggrin and
            lipid misprocessing
  JOURNAL   J Clin Invest 120 (3), 871-882 (2010)
   PUBMED   20179351
  REMARK    GeneRIF: ELA2 as a major new epidermal protease involved in
            essential pathways for skin barrier function.
REFERENCE   7  (bases 1 to 923)
  AUTHORS   Westmoreland,J.J., Wang,Q., Bouzaffour,M., Baker,S.J. and
            Sosa-Pineda,B.
  TITLE     Pdk1 activity controls proliferation, survival, and growth of
            developing pancreatic cells
  JOURNAL   Dev Biol 334 (1), 285-298 (2009)
   PUBMED   19635472
REFERENCE   8  (bases 1 to 923)
  AUTHORS   Krapp,A., Knofler,M., Ledermann,B., Burki,K., Berney,C.,
            Zoerkler,N., Hagenbuchle,O. and Wellauer,P.K.
  TITLE     The bHLH protein PTF1-p48 is essential for the formation of the
            exocrine and the correct spatial organization of the endocrine
            pancreas
  JOURNAL   Genes Dev 12 (23), 3752-3763 (1998)
   PUBMED   9851981
REFERENCE   9  (bases 1 to 923)
  AUTHORS   Petrucco,S., Wellauer,P.K. and Hagenbuchle,O.
  TITLE     The DNA-binding activity of transcription factor PTF1 parallels the
            synthesis of pancreas-specific mRNAs during mouse development
  JOURNAL   Mol Cell Biol 10 (1), 254-264 (1990)
   PUBMED   2294404
REFERENCE   10 (bases 1 to 923)
  AUTHORS   Stevenson,B.J., Hagenbuchle,O. and Wellauer,P.K.
  TITLE     Sequence organisation and transcriptional regulation of the mouse
            elastase II and trypsin genes
  JOURNAL   Nucleic Acids Res 14 (21), 8307-8330 (1986)
   PUBMED   3641189
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL928577.21, CJ042571.1,
            CJ042553.1 and CJ042539.1.
            
            On Jul 16, 2016 this sequence version replaced NM_007919.2.
            
            Summary: This gene encodes a serine protease enzyme that hydrolyzes
            elastin. This gene is highly expressed in the pancreatic acinar
            cells where the encoded preproprotein undergoes processing
            including signal peptide cleavage to generate an inactive zymogen.
            The removal of N-terminal activation peptide from the zymogen by
            trypsin generates active elastase enzyme. This gene is also
            expressed in the mouse epidermis where it participates in
            pro-filaggrin processing. [provided by RefSeq, Jul 2016].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026552.1, X04573.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AL928577.21        88764-88765         c
            3-473               CJ042571.1         2-472
            474-605             CJ042553.1         334-465
            606-913             CJ042539.1         145-452
            914-923             AL928577.21        77714-77723         c
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 74.75 cM"
     gene            1..923
                     /gene="Cela2a"
                     /gene_synonym="Ela-2; Ela2; Ela2a"
                     /note="chymotrypsin-like elastase family, member 2A"
                     /db_xref="GeneID:13706"
                     /db_xref="MGI:MGI:95316"
     exon            1..63
                     /gene="Cela2a"
                     /gene_synonym="Ela-2; Ela2; Ela2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..839
                     /gene="Cela2a"
                     /gene_synonym="Ela-2; Ela2; Ela2a"
                     /EC_number="3.4.21.71"
                     /note="elastase 2A"
                     /codon_start=1
                     /product="chymotrypsin-like elastase family member 2A
                     preproprotein"
                     /protein_id="NP_031945.1"
                     /db_xref="CCDS:CCDS18884.1"
                     /db_xref="GeneID:13706"
                     /db_xref="MGI:MGI:95316"
                     /translation="MIRTLLLSALVAGALSCGYPTYEVEDDVSRVVGGQEATPNTWPW
                     QVSLQVLSSGRWRHNCGGSLVANNWVLTAAHCLSNYQTYRVLLGAHSLSNPGAGSAAV
                     QVSKLVVHQRWNSQNVGNGYDIALIKLASPVTLSKNIQTACLPPAGTILPRNYVCYVT
                     GWGLLQTNGNSPDTLRQGRLLVVDYATCSSASWWGSSVKSSMVCAGGDGVTSSCNGDS
                     GGPLNCRASNGQWQVHGIVSFGSSLGCNYPRKPSVFTRVSNYIDWINSVMARN"
     exon            64..158
                     /gene="Cela2a"
                     /gene_synonym="Ela-2; Ela2; Ela2a"
                     /inference="alignment:Splign:2.1.0"
     exon            159..256
                     /gene="Cela2a"
                     /gene_synonym="Ela-2; Ela2; Ela2a"
                     /inference="alignment:Splign:2.1.0"
     exon            257..385
                     /gene="Cela2a"
                     /gene_synonym="Ela-2; Ela2; Ela2a"
                     /inference="alignment:Splign:2.1.0"
     exon            386..522
                     /gene="Cela2a"
                     /gene_synonym="Ela-2; Ela2; Ela2a"
                     /inference="alignment:Splign:2.1.0"
     exon            523..668
                     /gene="Cela2a"
                     /gene_synonym="Ela-2; Ela2; Ela2a"
                     /inference="alignment:Splign:2.1.0"
     exon            669..821
                     /gene="Cela2a"
                     /gene_synonym="Ela-2; Ela2; Ela2a"
                     /inference="alignment:Splign:2.1.0"
     exon            822..923
                     /gene="Cela2a"
                     /gene_synonym="Ela-2; Ela2; Ela2a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      893..898
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cela2a"
                     /gene_synonym="Ela-2; Ela2; Ela2a"
                     /note="hexamer: AATAAA"
     polyA_site      915
                     /gene="Cela2a"
                     /gene_synonym="Ela-2; Ela2; Ela2a"
                     /note="major polyA site"
ORIGIN      
        1 ctacagacgt ccagggacac accatgatca ggacactgct gctatctgcc ttggtggctg
       61 gagccctcag ctgtgggtac cccacttatg aggtggagga tgatgtgagc agggtagttg
      121 ggggtcaaga ggccacaccc aacacctggc cctggcaggt ctccctgcag gtcctttcct
      181 ccggaaggtg gcgccacaac tgcggaggct ccctggtggc caacaactgg gttctgacag
      241 ctgcccattg cctcagcaac tatcagacct accgagtgct gctgggcgca cacagcctct
      301 ccaaccccgg agctggctct gctgctgttc aagtctctaa gcttgtggtc caccagaggt
      361 ggaactccca aaacgtcggc aatggctatg acattgcctt aatcaaactg gccagcccag
      421 tgaccctgag caagaacatc cagacagctt gcctcccacc cgctggcacc attctcccga
      481 gaaactatgt ctgctatgtc acaggctggg gcctgctgca gaccaatggg aacagtcctg
      541 acaccctgag gcagggccgc ctgctggttg tggactatgc cacctgctcc agcgctagct
      601 ggtggggaag ctctgtgaag tccagcatgg tgtgcgctgg tggcgacggc gtgacctcca
      661 gctgcaatgg ggactctggc ggaccactga attgccgggc atctaatggc cagtggcagg
      721 tgcatggcat cgtgagcttc ggctcctctc tgggctgcaa ctacccccgc aagccatccg
      781 tcttcaccag ggtctccaac tacattgact ggatcaactc ggtgatggca aggaactaac
      841 tgaagacatt actgccactg tccccctgga aatgccatag aaaagaaata gtaataaagt
      901 aatttaagaa tcacatcctt gaa
//
