LOCUS       NM_144536               2638 bp    mRNA    linear   ROD 23-DEC-2022
DEFINITION  Mus musculus CDK5 regulatory subunit associated protein 1-like 1
            (Cdkal1), transcript variant 1, mRNA.
ACCESSION   NM_144536
VERSION     NM_144536.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Santos MCFD, Anderson CP, Neschen S, Zumbrennen-Bullough KB, Romney
            SJ, Kahle-Stephan M, Rathkolb B, Gailus-Durner V, Fuchs H, Wolf E,
            Rozman J, de Angelis MH, Cai WM, Rajan M, Hu J, Dedon PC and
            Leibold EA.
  TITLE     Irp2 regulates insulin production through iron-mediated
            Cdkal1-catalyzed tRNA modification
  JOURNAL   Nat Commun 11 (1), 296 (2020)
   PUBMED   31941883
  REMARK    GeneRIF: Cdkal1 catalyzes methylthiolation of t6A37 in tRNALysUUU
            to ms2t6A37.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Palmer CJ, Bruckner RJ, Paulo JA, Kazak L, Long JZ, Mina AI, Deng
            Z, LeClair KB, Hall JA, Hong S, Zushin PH, Smith KL, Gygi SP, Hagen
            S, Cohen DE and Banks AS.
  TITLE     Cdkal1, a type 2 diabetes susceptibility gene, regulates
            mitochondrial function in adipose tissue
  JOURNAL   Mol Metab 6 (10), 1212-1225 (2017)
   PUBMED   29031721
  REMARK    GeneRIF: Cdkal1 is necessary for normal mitochondrial morphology
            and function in adipose tissue. These results suggest that the type
            2 diabetes susceptibility gene CDKAL1 has novel functions in
            regulating mitochondrial activity.
REFERENCE   3  (bases 1 to 2638)
  AUTHORS   Take K, Waki H, Sun W, Wada T, Yu J, Nakamura M, Aoyama T, Yamauchi
            T and Kadowaki T.
  TITLE     CDK5 Regulatory Subunit-Associated Protein 1-like 1 Negatively
            Regulates Adipocyte Differentiation through Activation of Wnt
            Signaling Pathway
  JOURNAL   Sci Rep 7 (1), 7326 (2017)
   PUBMED   28779110
  REMARK    GeneRIF: Our results identify CDKAL1 as novel negative regulator of
            adipocyte differentiation and provide insights into the link
            between CDKAL1 and metabolic diseases such as type 2 diabetes and
            obesity.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2638)
  AUTHORS   Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK,
            Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L,
            Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre
            JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A,
            Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ,
            Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall
            S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA,
            Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR,
            Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana
            S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, MacArthur DG,
            Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC,
            Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL,
            de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown
            SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bucan M and Murray
            SA.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. PMID: 29144450]
REFERENCE   5  (bases 1 to 2638)
  AUTHORS   Okamura T, Yanobu-Takanashi R, Takeuchi F, Isono M, Akiyama K,
            Shimizu Y, Goto M, Liang YQ, Yamamoto K, Katsuya T, Fujioka A,
            Ohnaka K, Takayanagi R, Ogihara T, Yamori Y and Kato N.
  TITLE     Deletion of CDKAL1 affects high-fat diet-induced fat accumulation
            and glucose-stimulated insulin secretion in mice, indicating
            relevance to diabetes
  JOURNAL   PLoS One 7 (11), e49055 (2012)
   PUBMED   23173044
  REMARK    GeneRIF: CDKAL1 may affect such compensatory mechanisms regulating
            glucose homeostasis through interaction with diet
REFERENCE   6  (bases 1 to 2638)
  AUTHORS   O'Shaughnessy PJ, Abel M, Charlton HM, Hu B, Johnston H and Baker
            PJ.
  TITLE     Altered expression of genes involved in regulation of vitamin A
            metabolism, solute transportation, and cytoskeletal function in the
            androgen-insensitive tfm mouse testis
  JOURNAL   Endocrinology 148 (6), 2914-2924 (2007)
   PUBMED   17332066
REFERENCE   7  (bases 1 to 2638)
  AUTHORS   Evsikov AV, Graber JH, Brockman JM, Hampl A, Holbrook AE, Singh P,
            Eppig JJ, Solter D and Knowles BB.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   8  (bases 1 to 2638)
  AUTHORS   Bailey PJ, Klos JM, Andersson E, Karlen M, Kallstrom M, Ponjavic J,
            Muhr J, Lenhard B, Sandelin A and Ericson J.
  TITLE     A global genomic transcriptional code associated with CNS-expressed
            genes
  JOURNAL   Exp Cell Res 312 (16), 3108-3119 (2006)
   PUBMED   16919269
REFERENCE   9  (bases 1 to 2638)
  AUTHORS   Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M,
            Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A and
            Cortese R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 2638)
  AUTHORS   Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng
            EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC,
            Babbitt PC and Ferrin TE.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL513025.13, AK045506.1, AK082629.1, AK004490.1, AL589701.9 and
            DV651111.1.
            
            [WARNING] On Apr 17, 2023 this sequence was replaced by
            NM_144536.4.
            
            On May 7, 2015 this sequence version replaced NM_144536.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016073.1, SRR7652917.189158.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AL513025.13        58381-58390         c
            11-85               AK045506.1         2-76
            86-1207             AK082629.1         60-1181
            1208-1899           AK004490.1         9-700
            1900-2342           AL589701.9         92338-92780         c
            2343-2638           DV651111.1         25-320
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13"
     gene            1..2638
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /note="CDK5 regulatory subunit associated protein 1-like
                     1"
                     /db_xref="GeneID:68916"
                     /db_xref="MGI:MGI:1921765"
     exon            1..111
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            112..155
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..1892
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /EC_number="2.8.4.5"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tRNA-t(6)A37 methylthiotransferase;
                     threonylcarbamoyladenosine tRNA methylthiotransferase"
                     /codon_start=1
                     /product="threonylcarbamoyladenosine tRNA
                     methylthiotransferase isoform 1"
                     /protein_id="NP_653119.1"
                     /db_xref="CCDS:CCDS26412.1"
                     /db_xref="GeneID:68916"
                     /db_xref="MGI:MGI:1921765"
                     /translation="MPSASCDVLLDDIEDIISQEDSKPQDRQFSRKHVFPKVRRRNTQ
                     KYLQEEPRPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADL
                     WLLNSCTVKNPAEDHFRNSIKKAQEENKKVVLAGCVPQAQPRQDYLKGLSIIGVQQID
                     RVVEVVEETIKGHSVRLLGQKKDNGKRLGGARLDLPKIRKNPLIEIISINTGCLNACT
                     YCKTKHARGNLASYPIDELVERAKQSFQEGVCEIWLTSEDTGAYGRDIGTDLPTLLWK
                     LVEVIPEGAMLRLGMTNPPYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKR
                     EYCVADFKRVVDFLKEKVPGITIATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQ
                     FYPRPGTPAAKAEQVPAHVKKQRTKDLSRVFHSYNPYDHKIGERQQVLVTEESFDSKF
                     YVAHNRFYEQVLVPKNPAFMGKMVEVDIYESGKHFLKGQPVSETRVYTPSISKPLAKG
                     EVSGLTKEFRNRLGNHPNGTSDTCPATQHGSAYSRMVLQMSQYDCALKVATGLALLAL
                     LLHFWPDSLLTM"
     exon            156..325
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            326..438
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            439..523
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            524..620
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            621..669
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            670..790
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            791..894
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1061
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1207
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1388
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1451
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1535
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1700
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..2628
                     /gene="Cdkal1"
                     /gene_synonym="1190005B03Rik; 6620401C13Rik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagcatgcgc aaatcagccc ggcgggacac aagtgtcatg gcgctctcca tctgaagtct
       61 gtgcagtttc ctggcagggg cttgtggttt ttttcctctc agttcggaca gattcatctt
      121 tcaagaggac ttgagaccaa tttcagaaaa tcaagatgcc ttctgcgtct tgtgatgtac
      181 tgctggatga catagaagat atcatatcac aggaagattc aaagccacag gacagacagt
      241 tctcaagaaa gcatgtgttt cctaaagtac gaaggcggaa tacccaaaaa tatttgcaag
      301 aagagcccag gccaccaagt gacagcacta ttccaggcat acagaaaatt tggatccgaa
      361 catggggctg ctcacataat aattcggatg gagaatacat ggctggacag cttgctgcct
      421 atggctataa aattacagag aatgcatcag atgcagatct gtggctcctg aacagttgta
      481 ctgtgaaaaa tccagctgaa gaccacttta gaaactcaat caaaaaagct caagaagaga
      541 ataagaaagt tgtcctcgct ggctgtgttc cccaagccca gcctcgtcag gactacctca
      601 aaggactgag catcatcggg gttcagcaga tagatcgtgt ggtagaagtt gtggaggaaa
      661 caataaaagg tcactcagtg aggctcctgg gccagaagaa agacaacggg aagcgcttgg
      721 gtggagctcg actggatttg ccgaagatca gaaagaatcc gctgatagaa atcatttcca
      781 tcaacacggg gtgtctcaat gcttgtacct actgcaaaac taaacacgcc agaggaaacc
      841 tggccagcta tcccattgat gagcttgtgg agagagccaa gcaatccttt caagagggtg
      901 tttgtgagat atggctgacc agcgaggaca ccggggccta tggcagagac attggcacag
      961 atctccccac actcctgtgg aagttggtgg aagtgattcc tgagggagcc atgctgaggc
     1021 tgggcatgac aaatccacca tatattttag aacatctgga ggaaatggca aaaatcctca
     1081 accatcccag agtctacgct ttcctccaca tcccagtgca gtccgcctct gacagtgtgc
     1141 tcatggacat gaagagagag tactgcgtgg ccgacttcaa aagagtcgtg gattttctca
     1201 aggagaaggt tcctggaata acgattgcta cggatattat ctgtggtttt cctggagaaa
     1261 ccgatcagga ttttcaagaa acagtgaaac ttgttgaaga gtacaaattt ccaagcctct
     1321 tcatcaacca gttctatcca agacccggaa ctcctgctgc aaaagccgag caggttccag
     1381 cacatgtgaa aaaacaaagg acaaaagatc tttctcgggt gtttcattcc tacaatccat
     1441 acgatcacaa gattggcgaa agacaacaag tgctagtcac tgaagagtct tttgactcca
     1501 agttttatgt tgctcataac cgattctatg agcaggtttt agtaccaaag aaccctgcat
     1561 tcatggggaa aatggttgaa gtcgacattt acgaatcagg aaagcacttt ttgaaaggac
     1621 agccagtatc agaaaccaga gtgtatacac catccatcag caagccactg gccaagggag
     1681 aagtctcagg tttgacaaag gaattcagaa ataggcttgg gaatcatcca aacggaactt
     1741 cagacacctg tcctgcaact cagcatggct ctgcgtattc cagaatggtg ctgcagatgt
     1801 cccagtatga ctgcgcactg aaggtggcta caggcctggc tctcctcgct ctccttttgc
     1861 atttctggcc agactctttg cttacaatgt aactaagtaa agcctccata aggaataagt
     1921 ttctaattca agtggtcaac aaacaagagg acatcaacac cagttttcaa gagactgcct
     1981 tctgaactag acacactgcc ttgtctggat ctcttctgaa tgccctcaca ctgagctgag
     2041 ccatctgacc tcagaccaga tcacagcttc tgaacatgtc tttctcatca aactccttca
     2101 gacttcttcc taacaacaaa tacaagtggt tgggtttttt ggttggtttt tggttttttt
     2161 tgttttgttt tgtttttttt ttgttttttg ttttgttttt tttttttttt gtctccagtc
     2221 attgaatccc agcctaagtg cacagaggct ctgcctattc gtggctctgg ccatcctttc
     2281 cttgtgtgga agaacatcca gcccttaata aacattctcc cggctgtgtt tggagtcccc
     2341 gtgctacaaa catctttttc acagcagatg tgttcctaaa aagtttatat agaaacctat
     2401 tttgagaact tggacccaag tccaccactt aaaggactct tcctggcgtt ctagaaaatg
     2461 tgaagattcc atgcctgtgg cacccacaaa ctttcaaaca aaccatgatc ctttcctgac
     2521 acaccgacca ggcatgttac atttatcatt ttgacccaac accaccagcc gtctttacta
     2581 aatgaaatga cttctttcac aataaacaga attggtacaa taagaaaaaa aaaaaaaa
//
