LOCUS       NM_053180               1854 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus cyclin dependent kinase 20 (Cdk20), mRNA.
ACCESSION   NM_053180
VERSION     NM_053180.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Chaya,T., Maeda,Y., Tsutsumi,R., Ando,M., Ma,Y., Kajimura,N.,
            Tanaka,T. and Furukawa,T.
  TITLE     Ccrk-Mak/Ick signaling is a ciliary transport regulator essential
            for retinal photoreceptor survival
  JOURNAL   Life Sci Alliance 7 (11), e202402880 (2024)
   PUBMED   39293864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1854)
  AUTHORS   Lee,H. and Ko,H.W.
  TITLE     Cell cycle-related kinase is a crucial regulator for ciliogenesis
            and Hedgehog signaling in embryonic mouse lung development
  JOURNAL   BMB Rep 53 (7), 367-372 (2020)
   PUBMED   32317081
REFERENCE   4  (bases 1 to 1854)
  AUTHORS   Zhou,J., Liu,M., Sun,H., Feng,Y., Xu,L., Chan,A.W.H., Tong,J.H.,
            Wong,J., Chong,C.C.N., Lai,P.B.S., Wang,H.K., Tsang,S.W.,
            Goodwin,T., Liu,R., Huang,L., Chen,Z., Sung,J.J., Chow,K.L.,
            To,K.F. and Cheng,A.S.
  TITLE     Hepatoma-intrinsic CCRK inhibition diminishes myeloid-derived
            suppressor cell immunosuppression and enhances immune-checkpoint
            blockade efficacy
  JOURNAL   Gut 67 (5), 931-944 (2018)
   PUBMED   28939663
  REMARK    GeneRIF: Results delineate an immunosuppressive mechanism of the
            hepatoma-intrinsic CCRK signalling and highlight an overexpressed
            kinase target whose inhibition might empower hepatocellular
            carcinoma immunotherapy
REFERENCE   5  (bases 1 to 1854)
  AUTHORS   Lupu,F.I., Burnett,J.B. and Eggenschwiler,J.T.
  TITLE     Cell cycle-related kinase regulates mammalian eye development
            through positive and negative regulation of the Hedgehog pathway
  JOURNAL   Dev Biol 434 (1), 24-35 (2018)
   PUBMED   29166577
REFERENCE   6  (bases 1 to 1854)
  AUTHORS   Chaya,T., Omori,Y., Kuwahara,R. and Furukawa,T.
  TITLE     ICK is essential for cell type-specific ciliogenesis and the
            regulation of ciliary transport
  JOURNAL   EMBO J 33 (11), 1227-1242 (2014)
   PUBMED   24797473
REFERENCE   7  (bases 1 to 1854)
  AUTHORS   Feng,H., Cheng,A.S., Tsang,D.P., Li,M.S., Go,M.Y., Cheung,Y.S.,
            Zhao,G.J., Ng,S.S., Lin,M.C., Yu,J., Lai,P.B., To,K.F. and
            Sung,J.J.
  TITLE     Cell cycle-related kinase is a direct androgen receptor-regulated
            gene that drives beta-catenin/T cell factor-dependent
            hepatocarcinogenesis
  JOURNAL   J Clin Invest 121 (8), 3159-3175 (2011)
   PUBMED   21747169
  REMARK    GeneRIF: Cell cycle-related kinase is a direct androgen
            receptor-regulated gene that drives beta-catenin/T cell
            factor-dependent hepatocarcinogenesis
REFERENCE   8  (bases 1 to 1854)
  AUTHORS   Ko,H.W., Norman,R.X., Tran,J., Fuller,K.P., Fukuda,M. and
            Eggenschwiler,J.T.
  TITLE     Broad-minded links cell cycle-related kinase to cilia assembly and
            hedgehog signal transduction
  JOURNAL   Dev Cell 18 (2), 237-247 (2010)
   PUBMED   20159594
REFERENCE   9  (bases 1 to 1854)
  AUTHORS   Qiu,H., Dai,H., Jain,K., Shah,R., Hong,C., Pain,J., Tian,B.,
            Vatner,D.E., Vatner,S.F. and Depre,C.
  TITLE     Characterization of a novel cardiac isoform of the cell
            cycle-related kinase that is regulated during heart failure
  JOURNAL   J Biol Chem 283 (32), 22157-22165 (2008)
   PUBMED   18508765
  REMARK    GeneRIF: heart expresses a splice variant of CCRK, which promotes
            cardiac cell growth and survival; differs from the generic isoform
            in protein-protein interactions, substrate specificity and
            regulation of cell cycle; and is down-regulated in heart failure
REFERENCE   10 (bases 1 to 1854)
  AUTHORS   Wohlbold,L., Larochelle,S., Liao,J.C., Livshits,G., Singer,J.,
            Shokat,K.M. and Fisher,R.P.
  TITLE     The cyclin-dependent kinase (CDK) family member PNQALRE/CCRK
            supports cell proliferation but has no intrinsic CDK-activating
            kinase (CAK) activity
  JOURNAL   Cell Cycle 5 (5), 546-554 (2006)
   PUBMED   16552187
  REMARK    GeneRIF: Depletion of PNQALRE by >80% due to RNA interference
            (RNAi) impairs cell proliferation, but fails to arrest the cell
            cycle at a discrete point.
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC031907.1.
            
            On Dec 15, 2002 this sequence version replaced NM_053180.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031907.1, AY005133.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 33.26 cM"
     gene            1..1854
                     /gene="Cdk20"
                     /gene_synonym="4932702G04Rik; Ccrk; CDCH; p42; PNQLARE"
                     /note="cyclin dependent kinase 20"
                     /db_xref="GeneID:105278"
                     /db_xref="MGI:MGI:2145349"
     exon            1..118
                     /gene="Cdk20"
                     /gene_synonym="4932702G04Rik; Ccrk; CDCH; p42; PNQLARE"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..1084
                     /gene="Cdk20"
                     /gene_synonym="4932702G04Rik; Ccrk; CDCH; p42; PNQLARE"
                     /EC_number="2.7.11.22"
                     /note="cell division protein kinase 20; cell cycle-related
                     kinase; CDK-related protein kinase PNQLARE; CAK-kinase
                     p42; CDK-activating kinase p42; cyclin-dependent protein
                     kinase H; cyclin-kinase-activating kinase p42"
                     /codon_start=1
                     /product="cyclin-dependent kinase 20"
                     /protein_id="NP_444410.1"
                     /db_xref="CCDS:CCDS26601.1"
                     /db_xref="GeneID:105278"
                     /db_xref="MGI:MGI:2145349"
                     /translation="MDQYCILGRIGEGAHGIVFKAKHVETGEIVALKKVALRRLEDGI
                     PNQALREIKALQEIEDSQYVVQLKAVFPHGAGFVLAFEFMLSDLAEVVRHAQRPLAPA
                     QVKSYLQMLLKGVAFCHANNIVHRDLKPANLLISASGQLKIADFGLARVFSPDGGRLY
                     THQVATRWYRAPELLYGARQYDQGVDLWAVGCIMGELLNGSPLFPGENDIEQLCCVLR
                     ILGTPSPRVWPEITELPDYNKISFKEQAPVPLEEVLPDASPQALDLLGQFLLYPPRQR
                     IAASQALLHQYFFTAPLPAHPSELPIPQRPGGPAPKAHPGPPHVHDFHVDRPLEESLL
                     NPELIRPFIPEG"
     exon            119..232
                     /gene="Cdk20"
                     /gene_synonym="4932702G04Rik; Ccrk; CDCH; p42; PNQLARE"
                     /inference="alignment:Splign:2.1.0"
     exon            233..421
                     /gene="Cdk20"
                     /gene_synonym="4932702G04Rik; Ccrk; CDCH; p42; PNQLARE"
                     /inference="alignment:Splign:2.1.0"
     exon            422..543
                     /gene="Cdk20"
                     /gene_synonym="4932702G04Rik; Ccrk; CDCH; p42; PNQLARE"
                     /inference="alignment:Splign:2.1.0"
     exon            544..606
                     /gene="Cdk20"
                     /gene_synonym="4932702G04Rik; Ccrk; CDCH; p42; PNQLARE"
                     /inference="alignment:Splign:2.1.0"
     exon            607..730
                     /gene="Cdk20"
                     /gene_synonym="4932702G04Rik; Ccrk; CDCH; p42; PNQLARE"
                     /inference="alignment:Splign:2.1.0"
     exon            731..886
                     /gene="Cdk20"
                     /gene_synonym="4932702G04Rik; Ccrk; CDCH; p42; PNQLARE"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1827
                     /gene="Cdk20"
                     /gene_synonym="4932702G04Rik; Ccrk; CDCH; p42; PNQLARE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1807..1812
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cdk20"
                     /gene_synonym="4932702G04Rik; Ccrk; CDCH; p42; PNQLARE"
                     /note="hexamer: AATAAA"
     polyA_site      1827
                     /gene="Cdk20"
                     /gene_synonym="4932702G04Rik; Ccrk; CDCH; p42; PNQLARE"
                     /note="major polyA site"
ORIGIN      
        1 tgctggatcc ctgtagagta ggatctgggc accgcgggcc gacatggacc agtattgcat
       61 cctcggtcgc atcggggagg gcgcccacgg catcgtcttc aaagccaagc acgtagagac
      121 tggcgagatc gttgccctca agaaggtggc cttgcggcgg ctggaggatg gtattcctaa
      181 ccaggccctc agagaaatca aggctctgca ggagatagag gacagtcaat atgtggtgca
      241 gctgaaggct gtgttcccac atggtgcggg ctttgtgctg gctttcgaat ttatgctgtc
      301 ggacctggca gaggtggtgc gccatgccca gaggcccctg gccccagcac aagtcaagag
      361 ctacctgcag atgctgctca aaggtgttgc gttttgccat gccaacaaca ttgtgcatcg
      421 ggacctgaag cctgctaacc tgctcatcag tgcctcaggc cagctcaaga tagctgactt
      481 tggcctggcc cgggtcttct ctccggatgg tggtcgcctc tacacacatc aggtggccac
      541 caggtggtac cgagctcctg aactcctgta tggcgctcgg cagtatgacc agggcgttga
      601 cctatgggct gtgggctgca tcatgggaga gctgttgaat gggtcccccc tgttcccggg
      661 cgaaaacgac attgaacaac tgtgctgtgt gcttcgcatc ctgggtaccc cgagtcctcg
      721 agtctggccg gagatcacag agctgcctga ctacaacaag atctccttca aggagcaggc
      781 accagtgccc ctggaggagg tgctgcctga tgcctctccc caggccttgg acctgctggg
      841 ccagttcctc ctctaccctc cacgacagcg tattgcagcc tcccaggccc ttctgcatca
      901 gtacttcttc acagcgcctc tgcctgccca tccatccgag ctgccaattc ctcagcgccc
      961 agggggacct gcacccaagg ctcacccagg gcccccccat gtccacgact tccatgtgga
     1021 tcgacctctt gaggagtcac tgttgaaccc agaactgatt cggcccttca tcccagaggg
     1081 gtgagatgct ggtccaggcc ttcctgctcg ccctaggagc acctctttct gatttgcctc
     1141 catggcctcc ccacggctat atataccaca cctggtcctg ctccttagtg tgcttgaggg
     1201 ctgggctctg ggaggcagaa ccgtgagatg ttcatcccag cagagaaaga gactcacgtc
     1261 ctacagacaa agcctccaga aactgctagc tgtgtccttc tccagggcca cccctcagtg
     1321 gtgccacccg gccttagaga tgattgtcag gctctgtccc ctcttcaagg acattggtac
     1381 tacagcacca cctggtggaa gcacagagta taagctgtct tcataccggg gacacagctg
     1441 ggaagtcaga catgttttag ttttggttcc actgggtcag gatttgaggt tcatataaaa
     1501 gccctgggtg tttctgtcta attgcacctt gtctgttgct gttagggaaa ggacaatggt
     1561 gggccttgat tcacaggggt caggtactca gaagaggcct cctgtgaagg ccatttgggt
     1621 cctcaggctt cccatgctat tcacgggact tgagtgctca tttgggagcg agagtccaga
     1681 agctgaggcc cagggatgga cagtccagtt cccgaagcac acttcccaca tgtcgggtgg
     1741 gtcagtcagt gagcctgagg ctgccttgca gatgcggaag cagacattcc tggaatccac
     1801 tcagtaaata aattccagtg tgactcaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//
