LOCUS       NM_133654               2342 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus CD34 antigen (Cd34), transcript variant 2, mRNA.
ACCESSION   NM_133654
VERSION     NM_133654.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Chen,X., Tober,J., Dominguez,M., Tang,A.T., Bockman,J., Yang,J.,
            Mani,S., Lee,C.N., Chen,M., Thillaikumaran,T., Mericko-Ishizuka,P.,
            Mainigi,M., Speck,N.A. and Kahn,M.L.
  TITLE     Lineage tracing studies suggest that the placenta is not a de novo
            source of hematopoietic stem cells
  JOURNAL   PLoS Biol 23 (1), e3003003 (2025)
   PUBMED   39874373
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Ghotbi,E., Tchegnon,E., Chen,Z., Li,S., Shipman,T., Wang,Y.,
            Raman,J., Zhang,Y., McKay,R.M., Liao,C.P. and Le,L.Q.
  TITLE     Transcription factor KROX20 marks epithelial stem cell ancestors
            for hair follicle formation
  JOURNAL   J Clin Invest 134 (23), e180160 (2024)
   PUBMED   39361422
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2342)
  AUTHORS   Olczak,A., Pieczonka,T.D., Lawicki,S., Lukaszyk,K.,
            Pulawska-Czub,A., Cambier,L. and Kobielak,K.
  TITLE     The overexpression of R-spondin 3 affects hair morphogenesis and
            hair development along with the formation and maturation of the
            hair follicle stem cells
  JOURNAL   Front Physiol 15, 1424077 (2024)
   PUBMED   39351282
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2342)
  AUTHORS   Zhu,Z., Zou,Q., Wang,C., Li,D., Yang,Y., Xiao,Y., Jin,Y., Yan,J.,
            Luo,L., Sun,Y. and Liang,X.
  TITLE     Isl Identifies the Extraembryonic Mesodermal/Allantois Progenitors
            and is Required for Placenta Morphogenesis and Vasculature
            Formation
  JOURNAL   Adv Sci (Weinh) 11 (32), e2400238 (2024)
   PUBMED   38923264
REFERENCE   5  (bases 1 to 2342)
  AUTHORS   Flaherty,S.E. 3rd, Bezy,O., Paulhus,B.L., Song,L., Piper,M.,
            Pang,J., Park,Y., Asano,S., Lien,Y.C., Griffin,J.D., Robertson,A.,
            Opsahl,A., Shanthappa,D.H., Ahn,Y., Pashos,E., Simmons,R.A.,
            Birnbaum,M.J. and Wu,Z.
  TITLE     SPAG7 deletion causes intrauterine growth restriction, resulting in
            adulthood obesity and metabolic dysfunction
  JOURNAL   Elife 12, 91114 (2024)
   PUBMED   39056292
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2342)
  AUTHORS   Krause,D.S., Ito,T., Fackler,M.J., Smith,O.M., Collector,M.I.,
            Sharkis,S.J. and May,W.S.
  TITLE     Characterization of murine CD34, a marker for hematopoietic
            progenitor and stem cells
  JOURNAL   Blood 84 (3), 691-701 (1994)
   PUBMED   7519070
REFERENCE   7  (bases 1 to 2342)
  AUTHORS   Suda,J., Sudo,T., Ito,M., Ohno,N., Yamaguchi,Y. and Suda,T.
  TITLE     Two types of murine CD34 mRNA generated by alternative splicing
  JOURNAL   Blood 79 (9), 2288-2295 (1992)
   PUBMED   1373970
REFERENCE   8  (bases 1 to 2342)
  AUTHORS   Taketo,M., Howard,T.A. and Seldin,M.F.
  TITLE     Mapping of recombinant retrovirus integration sites that cause
            expression of the viral genome in murine embryonal carcinoma cells
  JOURNAL   Mamm Genome 2 (4), 240-245 (1992)
   PUBMED   1543915
REFERENCE   9  (bases 1 to 2342)
  AUTHORS   Howell,S.M., Molgaard,H.V., Greaves,M.F. and Spurr,N.K.
  TITLE     Localisation of the gene coding for the haemopoietic stem cell
            antigen CD34 to chromosome 1q32
  JOURNAL   Hum Genet 87 (5), 625-627 (1991)
   PUBMED   1717362
REFERENCE   10 (bases 1 to 2342)
  AUTHORS   Brown,J., Greaves,M.F. and Molgaard,H.V.
  TITLE     The gene encoding the stem cell antigen, CD34, is conserved in
            mouse and expressed in haemopoietic progenitor cell lines, brain,
            and embryonic fibroblasts
  JOURNAL   Int Immunol 3 (2), 175-184 (1991)
   PUBMED   1709048
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC162692.2.
            
            On Oct 18, 2022 this sequence version replaced NM_133654.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006607.1, AK053874.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-114               AC162692.2         84513-84626         c
            115-282             AC162692.2         75551-75718         c
            283-542             AC162692.2         73993-74252         c
            543-623             AC162692.2         72538-72618         c
            624-780             AC162692.2         64984-65140         c
            781-833             AC162692.2         64695-64747         c
            834-998             AC162692.2         64327-64491         c
            999-2342            AC162692.2         62278-63621         c
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 98.38 cM"
     gene            1..2342
                     /gene="Cd34"
                     /note="CD34 antigen"
                     /db_xref="GeneID:12490"
                     /db_xref="MGI:MGI:88329"
     exon            1..114
                     /gene="Cd34"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..1184
                     /gene="Cd34"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; hematopoietic progenitor cell antigen CD34;
                     cluster designation 34"
                     /codon_start=1
                     /product="hematopoietic progenitor cell antigen CD34
                     isoform 2 precursor"
                     /protein_id="NP_598415.1"
                     /db_xref="CCDS:CCDS15640.1"
                     /db_xref="GeneID:12490"
                     /db_xref="MGI:MGI:88329"
                     /translation="MQVHRDTRAGLLLPWRWVALCLMSLLHLNNLTSATTETSTQGIS
                     PSVPTNESVEENITSSIPGSTSHYLIYQDSSKTTPAISETMVNFTVTSGIPSGSGTPH
                     TFSQPQTSPTGILPTTSDSISTSEMTWKSSLPSINVSDYSPNNSSFEMTSPTEPYAYT
                     SSSAPSAIKGEIKCSGIREVRLAQGICLELSEASSCEEFKKEKGEDLIQILCEKEEAE
                     ADAGASVCSLLLAQSEVRPECLLMVLANSTELPSKLQLMEKHQSDLRKLGIQSFNKQD
                     IGSHQSYSRKTLIALVTSGVLLAILGTTGYFLMNRRSWSPTGERLGEDPYYTENGGGQ
                     GYSSGPGASPETQGKANVTRGAQENGTGQATSRNGHSARQHVVADTEL"
     exon            115..282
                     /gene="Cd34"
                     /inference="alignment:Splign:2.1.0"
     exon            283..542
                     /gene="Cd34"
                     /inference="alignment:Splign:2.1.0"
     exon            543..623
                     /gene="Cd34"
                     /inference="alignment:Splign:2.1.0"
     exon            624..780
                     /gene="Cd34"
                     /inference="alignment:Splign:2.1.0"
     exon            781..833
                     /gene="Cd34"
                     /inference="alignment:Splign:2.1.0"
     exon            834..998
                     /gene="Cd34"
                     /inference="alignment:Splign:2.1.0"
     exon            999..2342
                     /gene="Cd34"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2295..2300
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cd34"
                     /note="hexamer: AATAAA"
     polyA_site      2318
                     /gene="Cd34"
                     /note="major polyA site"
     regulatory      2323..2328
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cd34"
                     /note="hexamer: ATTAAA"
     polyA_site      2342
                     /gene="Cd34"
ORIGIN      
        1 ctgcaccgag cgcatctccg gagcggtaca ggagaatgca ggtccacagg gacacgcgcg
       61 cggggctcct gctgccatgg cgctgggtag ctctctgcct gatgagtctg ctgcatctaa
      121 ataacttgac ttctgctacc acggagactt ctacacaagg aatatcccca tcagttccta
      181 ccaatgagtc tgttgaggaa aatatcacat ctagcatccc tggaagtacc agccactact
      241 tgatctatca ggacagcagt aagaccacac cagccatctc agagactatg gtcaacttta
      301 cagttacctc tgggatccct tcaggctctg gaactccaca cactttttca caaccacaga
      361 cttccccaac tggcatactg cctactactt cagacagtat ttccacttca gagatgacct
      421 ggaagtccag cctgccatct ataaatgttt ctgattattc gcctaataat agcagctttg
      481 agatgacatc acccaccgag ccatatgctt acacatcatc ttctgctccg agtgccatta
      541 agggagaaat caaatgctct ggaatccgag aagtgaggtt ggcccagggt atctgcctgg
      601 aactaagtga agcatctagt tgtgaggagt ttaagaagga aaagggagaa gatctaattc
      661 aaatactgtg tgaaaaggag gaggctgagg ctgatgctgg tgctagtgtc tgctccctgc
      721 ttctagccca gtctgaggtt aggcctgagt gtttgctgat ggtcttggcc aatagcacag
      781 aacttcccag caaactccag cttatggaaa agcaccaatc tgacttgaga aagctgggga
      841 tccaaagctt caataaacaa gatatcggga gccaccagag ctattcccga aagactctta
      901 ttgcattggt cacctctgga gttctgctgg ccatcttggg caccactggt tatttcctga
      961 tgaaccgtcg cagttggagc cctacaggag aaaggctggg tgaagaccct tattacacgg
     1021 agaatggtgg aggccagggc tatagctcag gacctggggc ctcccctgag actcagggaa
     1081 aggccaatgt gacccgaggg gctcaggaga acgggaccgg ccaggccact tccagaaacg
     1141 gccattcagc aagacaacat gtggtggctg acacagaact gtgatttggt tgggtgggca
     1201 actgggtggt atgcaggaaa gtggcatctc ttgtctctga cttcatgctg ccttcagctc
     1261 atgtccggcc ttctcctatt acatacactt ctgaaactgt tcctgggact cttcaccttg
     1321 gggaaggcag ataaactgcc ttctgcacat tcaacttcct gaatccaatc tctgaccttt
     1381 gggtcaagtt gtggtgggaa gaagcctagg tctagaggag ctgccaaaaa agttggtggc
     1441 tatgtagcac ttgccctgga cccatttctc ctctctcgcc tcttcacggg aactctccgg
     1501 aagactagct tttctaagct accacttctt cccaggaaac tttgctattt ttactgcttc
     1561 ttcccctact ttatggaaac caaggtattc actgacatgt gctcccttgc aagggtacag
     1621 ccagaaaagt gctattttaa aatacatcct taaaaaatgc atcccttata acttcaagac
     1681 actgtggatt tagtcaccaa cttctatctt gttcacctgt tcctgaatgt ctgtctacag
     1741 aggccaggac aactttctgt ctggagtctg ctcaatgttt tagagcaaca gctcaatctg
     1801 atcccttggg cccacacaga aatctcattg gttcaaccta gacaggacag tggaattaga
     1861 ctttgaactg agcctctgtt ttttgtttta ttttattgct ggggtttgaa cccagagctt
     1921 cacacagctt ctttaggctt ccaagtagct tgagctacca ggcccagctg agctaaacct
     1981 cctgacctga gctcttcaaa ggaatactct tgctctgagg cccttggcct tctctaaatt
     2041 acgtgacttc ccccttcctc tgactcctgg gggagctgtg gcctcagtcc cctggcagat
     2101 tcctttcagt ctgtgccttt cctagtccaa accccttcac tattttataa ccctttgtga
     2161 tcagaggttc agaatatcta caaagactat aagcttcctc tcctggggtt aaggggagaa
     2221 caggggtcct gattttaatg atggctagga acaaaacttt ccagagatga gaggattggg
     2281 tgtattctct tctgaataaa cgtgatgagt gaaaatgatg taattaaatt gatgatgaaa
     2341 ta
//
