LOCUS       NM_021293               2488 bp    mRNA    linear   ROD 28-MAY-2024
DEFINITION  Mus musculus CD33 molecule (Cd33), transcript variant 2, mRNA.
ACCESSION   NM_021293
VERSION     NM_021293.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Tan,S., Gao,H., Sun,J., Li,N., Zhang,Y., Yang,L., Wang,M., Wang,Q.
            and Zhai,Q.
  TITLE     CD33/TREM2 Signaling Mediates Sleep Deprivation-Induced Memory
            Impairment by Regulating Microglial Phagocytosis
  JOURNAL   Neuromolecular Med 25 (2), 255-271 (2023)
   PUBMED   36639554
  REMARK    GeneRIF: CD33/TREM2 Signaling Mediates Sleep Deprivation-Induced
            Memory Impairment by Regulating Microglial Phagocytosis.
REFERENCE   3  (bases 1 to 2488)
  AUTHORS   Wang,X., Liu,M., Zhang,J., Brown,N.K., Zhang,P., Zhang,Y., Liu,H.,
            Du,X., Wu,W., Devenport,M., Tao,W., Mao-Draayer,Y., Chen,G.Y.,
            Chen,Y.E., Zheng,P. and Liu,Y.
  TITLE     CD24-Siglec axis is an innate immune checkpoint against
            metaflammation and metabolic disorder
  JOURNAL   Cell Metab 34 (8), 1088-1103 (2022)
   PUBMED   35921817
REFERENCE   4  (bases 1 to 2488)
  AUTHORS   Gnoth,K., Geissler,S., Feldhaus,J., Taudte,N., Ilse,V., Zurner,S.,
            Greiser,S., Braumann,U.D., Rahfeld,J.U., Cynis,H. and Schilling,S.
  TITLE     Evidence for Enhanced Efficacy of Passive Immunotherapy against
            Beta-Amyloid in CD33-Negative 5xFAD Mice
  JOURNAL   Biomolecules 12 (3), 399 (2022)
   PUBMED   35327591
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2488)
  AUTHORS   Griciuc,A., Federico,A.N., Natasan,J., Forte,A.M., McGinty,D.,
            Nguyen,H., Volak,A., LeRoy,S., Gandhi,S., Lerner,E.P., Hudry,E.,
            Tanzi,R.E. and Maguire,C.A.
  TITLE     Gene therapy for Alzheimer's disease targeting CD33 reduces amyloid
            beta accumulation and neuroinflammation
  JOURNAL   Hum Mol Genet 29 (17), 2920-2935 (2020)
   PUBMED   32803224
  REMARK    GeneRIF: Gene therapy for Alzheimer's disease targeting CD33
            reduces amyloid beta accumulation and neuroinflammation.
REFERENCE   6  (bases 1 to 2488)
  AUTHORS   Orr,S.J., Morgan,N.M., Elliott,J., Burrows,J.F., Scott,C.J.,
            McVicar,D.W. and Johnston,J.A.
  TITLE     CD33 responses are blocked by SOCS3 through accelerated
            proteasomal-mediated turnover
  JOURNAL   Blood 109 (3), 1061-1068 (2007)
   PUBMED   17008544
  REMARK    GeneRIF: SOCS3 inhibited the CD33-induced block on cytokine-induced
            proliferation. SOCS3 and its target protein CD33 are degraded
            concomitantly concomitantly.
REFERENCE   7  (bases 1 to 2488)
  AUTHORS   Brinkman-Van der Linden,E.C., Angata,T., Reynolds,S.A.,
            Powell,L.D., Hedrick,S.M. and Varki,A.
  TITLE     CD33/Siglec-3 binding specificity, expression pattern, and
            consequences of gene deletion in mice
  JOURNAL   Mol Cell Biol 23 (12), 4199-4206 (2003)
   PUBMED   12773563
  REMARK    GeneRIF: mCD33 is expressed on myeloid precursors in the bone
            marrow
REFERENCE   8  (bases 1 to 2488)
  AUTHORS   Yu,Z., Maoui,M., Wu,L., Banville,D. and Shen,S.
  TITLE     mSiglec-E, a novel mouse CD33-related siglec (sialic acid-binding
            immunoglobulin-like lectin) that recruits Src homology 2
            (SH2)-domain-containing protein tyrosine phosphatases SHP-1 and
            SHP-2
  JOURNAL   Biochem J 353 (Pt 3), 483-492 (2001)
   PUBMED   11171044
REFERENCE   9  (bases 1 to 2488)
  AUTHORS   Stubbs,L., Carver,E.A., Shannon,M.E., Kim,J., Geisler,J.,
            Generoso,E.E., Stanford,B.G., Dunn,W.C., Mohrenweiser,H.,
            Zimmermann,W., Watt,S.M. and Ashworth,L.K.
  TITLE     Detailed comparative map of human chromosome 19q and related
            regions of the mouse genome
  JOURNAL   Genomics 35 (3), 499-508 (1996)
   PUBMED   8812484
REFERENCE   10 (bases 1 to 2488)
  AUTHORS   Tchilian,E.Z., Beverley,P.C., Young,B.D. and Watt,S.M.
  TITLE     Molecular cloning of two isoforms of the murine homolog of the
            myeloid CD33 antigen
  JOURNAL   Blood 83 (11), 3188-3198 (1994)
   PUBMED   8193354
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC132379.1 and AK155745.1.
            
            On Dec 1, 2007 this sequence version replaced NM_021293.2.
            
            Transcript Variant: This variant (2) lacks an exon in the 3' coding
            region that results in a frameshift compared to variant 1. The
            resulting protein (isoform 2) is shorter and has a distinct
            C-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK155745.1, S71403.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164133, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1225              BC132379.1         1-1225
            1226-1586           AK155745.1         1157-1517
            1587-1793           BC132379.1         1587-1793
            1794-1846           AK155745.1         1727-1779
            1847-1909           BC132379.1         1847-1909
            1910-2488           AK155745.1         1843-2421
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 28.25 cM"
     gene            1..2488
                     /gene="Cd33"
                     /gene_synonym="gp67; Siglec-3"
                     /note="CD33 molecule"
                     /db_xref="GeneID:12489"
                     /db_xref="MGI:MGI:99440"
     exon            1..114
                     /gene="Cd33"
                     /gene_synonym="gp67; Siglec-3"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..1082
                     /gene="Cd33"
                     /gene_synonym="gp67; Siglec-3"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; myeloid cell surface antigen CD33; sialic
                     acid-binding Ig-like lectin 3; CD33 antigen"
                     /codon_start=1
                     /product="myeloid cell surface antigen CD33 isoform 2
                     precursor"
                     /protein_id="NP_067268.1"
                     /db_xref="CCDS:CCDS21173.1"
                     /db_xref="GeneID:12489"
                     /db_xref="MGI:MGI:99440"
                     /translation="MLWPLPLFLLCAGSLAQDLEFQLVAPESVTVEEGLCVHVPCSVF
                     YPSIKLTLGPVTGSWLRKGVSLHEDSPVATSDPRQLVQKATQGRFQLLGDPQKHDCSL
                     FIRDAQKNDTGMYFFRVVREPFVRYSYKKSQLSLHVTSLSRTPDIIIPGTLEAGYPSN
                     LTCSVPWACEQGTPPTFSWMSTALTSLSSRTTDSSVLTFTPQPQDHGTKLTCLVTFSG
                     AGVTVERTIQLNVTRKSGQMRELVLVAVGEATVKLLILGLCLVFLIVMFCRRKTTKLS
                     VHMGCENPIKAHQQDSKVHSNPENPRPLQKDSPQEQSSVHTKISLDFMGGKPQEYSEI
                     "
     exon            115..495
                     /gene="Cd33"
                     /gene_synonym="gp67; Siglec-3"
                     /inference="alignment:Splign:2.1.0"
     exon            496..774
                     /gene="Cd33"
                     /gene_synonym="gp67; Siglec-3"
                     /inference="alignment:Splign:2.1.0"
     exon            775..868
                     /gene="Cd33"
                     /gene_synonym="gp67; Siglec-3"
                     /inference="alignment:Splign:2.1.0"
     exon            869..935
                     /gene="Cd33"
                     /gene_synonym="gp67; Siglec-3"
                     /inference="alignment:Splign:2.1.0"
     exon            936..2488
                     /gene="Cd33"
                     /gene_synonym="gp67; Siglec-3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1542..1547
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cd33"
                     /gene_synonym="gp67; Siglec-3"
                     /note="hexamer: AATAAA"
     polyA_site      1574
                     /gene="Cd33"
                     /gene_synonym="gp67; Siglec-3"
                     /note="major polyA site"
     regulatory      1811..1816
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cd33"
                     /gene_synonym="gp67; Siglec-3"
                     /note="hexamer: ATTAAA"
     polyA_site      1833
                     /gene="Cd33"
                     /gene_synonym="gp67; Siglec-3"
                     /note="major polyA site"
ORIGIN      
        1 acccatgaca ggaagcgtca accacccaac cttccttccc cttttacttg taggaaccta
       61 ggaccattca ctgtgctatg ctgtggccac tgccgctgtt cttgctgtgt gcaggctccc
      121 tggctcagga tttagaattc cagctggtgg cgcccgagtc agtgacagtc gaggagggcc
      181 tatgtgtcca tgtgccctgc agtgttttct acccctccat taagctcact ttaggacctg
      241 tgaccggctc ctggctccgg aaaggggtca gtctccatga agactctcca gtggccacaa
      301 gtgaccccag acaactagtg cagaaggcaa cacagggcag attccaactc cttggggacc
      361 cacagaaaca tgactgttcc ctgttcatca gagatgcaca gaaaaatgac acaggaatgt
      421 acttcttcag agtggtcaga gaaccttttg tgagatattc ttacaaaaaa agccagctgt
      481 cactgcatgt gacctctcta tcacggactc ctgacattat aatcccgggg accctggagg
      541 ctggctatcc tagcaatctc acctgctctg tgccctgggc ttgtgagcag gggacacccc
      601 ctactttctc ctggatgtca actgccctca cctccttgag ttcccgaacc acagactcct
      661 ccgtgctgac gttcacacct cagcctcagg accatggtac caaactcacc tgcttggtga
      721 ccttctctgg agcaggtgtc actgtggaaa ggaccatcca gctcaatgtt acccggaaat
      781 caggccagat gagagagctg gtcctggtgg ctgtggggga ggcaacggtc aagctcctga
      841 ttcttgggct ctgtctcgtg tttctcattg tgatgttctg cagaaggaag acaacaaagc
      901 tgtcagtgca catgggctgt gaaaatccta tcaaggctca tcagcaggac tccaaggtgc
      961 acagcaatcc tgagaatcca agacccttac agaaggactc acctcaggaa cagagcagtg
     1021 tccacactaa gatttccttg gatttcatgg gagggaagcc tcaggagtac tcagagatct
     1081 aaatgcacca agctttgctc tcctgtgaag aacctgtgtc ctctatggct tcctgtggat
     1141 aattcctgca tccctctgat cccagagtgg gttatgctgc tgtgtgtgag tcttacactc
     1201 agttgacatc atctgacttt gcaataagag acccgggaca gagtctgtgc tccttcattt
     1261 atatccccac aagttgttcc ctctattttt ggtttttgta ctcagtataa tgatccccta
     1321 ccttctcctc ctgcctcacc tctttctcct tctctctttc cacatctctt atatggatga
     1381 atatacacat gtgtgtgcca aggcccttgc ccatgagtaa gtacatgtat gaaggctaga
     1441 ggtcaaagtt cagtgccttc ctcttttttc tcatcattat cactcttcat catcactatc
     1501 gtcatcatta ttattattct gacacagggt atggtggttt gaataaaaat ggcccccata
     1561 gggcacaatt aggaggtgtg gccttgttga ataggtgtgg cctagttgga gaaagtgtgc
     1621 cactggggct gggctttggg gttttaaatg ctcaagccag gcccagtgtc actttatttc
     1681 ctgatgtctg ccaatccaga aattgaactc tcagctacct atctatctag caccatgtct
     1741 atctgcatgt tgctgtgctt cccgctatga agataaagga ctaaacctct gaacctgtaa
     1801 gccatctcca attaaacgtt ttcctttata agagttgctg ttgtcacagt atatctacac
     1861 agcaattgaa ccctaactaa agcacaagct ctccctgagc ctggagcttg ctgtttaggc
     1921 aagactggct ggacagtgag tttctggggt cacccttttc tgacctccaa tattttgatg
     1981 agagttatag actgtcatat gtagctttat gtgagtgctt tattgctagg gctcctttct
     2041 ctcttgctca ggaacctcac ctttcactgg ccaggaaaga gggagacttg gagtaataac
     2101 acttttattg aatcaggaga agagagtcac actaacaata ggaaaatctt ttacccaagc
     2161 aaatatgcac aaacacacat acatttatac acacgttcat acctgcacat tcattatctc
     2221 atacttattt gttgggtcta gcaatctgtc tctgtctcat caacttacat acttacacac
     2281 gcaaatagtt acacacacac acacacacac acacacacag agagagagag agagagagag
     2341 agagagagag agagagagag agagagaaac gggggtgggg gggcaaaggc ctccatgagc
     2401 aggagcaggt tttattgagc tagtgcaaaa agaactcact cattctggat gaaaaataag
     2461 ctccaacctt tattgctcag agaaaggg
//
