LOCUS       NM_009847               5423 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus CD2-associated protein (Cd2ap), transcript variant 1,
            mRNA.
ACCESSION   NM_009847
VERSION     NM_009847.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Zhang,L., Huang,L., Zhou,Y., Meng,J., Zhang,L., Zhou,Y., Zheng,N.,
            Guo,T., Zhao,S., Wang,Z., Huo,Y., Zhao,Y., Chen,X.F., Zheng,H.,
            Holtzman,D.M. and Zhang,Y.W.
  TITLE     Microglial CD2AP deficiency exerts protection in an Alzheimer's
            disease model of amyloidosis
  JOURNAL   Mol Neurodegener 19 (1), 95 (2024)
   PUBMED   39695808
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5423)
  AUTHORS   Wang,X., Zhang,Y., Yu,J., Ma,Y., Xu,Y., Shi,J., Qi,Z. and Liu,X.
  TITLE     Identification and analysis of key circRNAs in the mouse embryonic
            ovary provides insight into primordial follicle development
  JOURNAL   BMC Genomics 25 (1), 139 (2024)
   PUBMED   38310234
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5423)
  AUTHORS   Shen,R., Zhao,X., He,L., Ding,Y., Xu,W., Lin,S., Fang,S., Yang,W.,
            Sung,K., Spencer,B., Rissman,R.A., Lei,M., Ding,J. and Wu,C.
  TITLE     Upregulation of RIN3 induces endosomal dysfunction in Alzheimer's
            disease
  JOURNAL   Transl Neurodegener 9 (1), 26 (2020)
   PUBMED   32552912
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5423)
  AUTHORS   Pandey,R.S., Graham,L., Uyar,A., Preuss,C., Howell,G.R. and
            Carter,G.W.
  TITLE     Genetic perturbations of disease risk genes in mice capture
            transcriptomic signatures of late-onset Alzheimer's disease
  JOURNAL   Mol Neurodegener 14 (1), 50 (2019)
   PUBMED   31878951
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5423)
  AUTHORS   Shih,N.Y., Li,J., Cotran,R., Mundel,P., Miner,J.H. and Shaw,A.S.
  TITLE     CD2AP localizes to the slit diaphragm and binds to nephrin via a
            novel C-terminal domain
  JOURNAL   Am J Pathol 159 (6), 2303-2308 (2001)
   PUBMED   11733379
  REMARK    GeneRIF: CD2AP is expressed in glomerular podocytes, and may play a
            role in a specialized adhesion complex known as the slit diaphragm.
REFERENCE   7  (bases 1 to 5423)
  AUTHORS   Lehtonen,S., Ora,A., Olkkonen,V.M., Geng,L., Zerial,M., Somlo,S.
            and Lehtonen,E.
  TITLE     In vivo interaction of the adapter protein CD2-associated protein
            with the type 2 polycystic kidney disease protein, polycystin-2
  JOURNAL   J Biol Chem 275 (42), 32888-32893 (2000)
   PUBMED   10913159
REFERENCE   8  (bases 1 to 5423)
  AUTHORS   Shih,N.Y., Li,J., Karpitskii,V., Nguyen,A., Dustin,M.L.,
            Kanagawa,O., Miner,J.H. and Shaw,A.S.
  TITLE     Congenital nephrotic syndrome in mice lacking CD2-associated
            protein
  JOURNAL   Science 286 (5438), 312-315 (1999)
   PUBMED   10514378
REFERENCE   9  (bases 1 to 5423)
  AUTHORS   Dustin,M.L., Olszowy,M.W., Holdorf,A.D., Li,J., Bromley,S.,
            Desai,N., Widder,P., Rosenberger,F., van der Merwe,P.A., Allen,P.M.
            and Shaw,A.S.
  TITLE     A novel adaptor protein orchestrates receptor patterning and
            cytoskeletal polarity in T-cell contacts
  JOURNAL   Cell 94 (5), 667-677 (1998)
   PUBMED   9741631
REFERENCE   10 (bases 1 to 5423)
  AUTHORS   Jansson,S., Olkkonen,V., Martin-Parras,L., Chavrier,P.,
            Stapleton,M., Zerial,M. and Lehtonen,E.
  TITLE     Mouse metanephric kidney as a model system for identifying
            developmentally regulated genes
  JOURNAL   J Cell Physiol 173 (2), 147-151 (1997)
   PUBMED   9365514
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC111082.23.
            
            On Aug 8, 2022 this sequence version replaced NM_009847.3.
            
            Summary: This gene encodes a scaffolding molecule that regulates
            the actin cytoskeleton. The protein directly interacts with
            filamentous actin and a variety of cell membrane proteins through
            multiple actin binding sites, SH3 domains, and a proline-rich
            region containing binding sites for SH3 domains. The cytoplasmic
            protein localizes to membrane ruffles, lipid rafts, and the leading
            edges of cells. It is implicated in dynamic actin remodeling and
            membrane trafficking that occurs during receptor endocytosis and
            cytokinesis. The mouse genome contains at least two pseudogenes
            located on chromosomes 9 and 17. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ459109.1, BC157901.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-136               AC111082.23        138598-138733       c
            137-297             AC111082.23        114653-114813       c
            298-451             AC111082.23        107611-107764       c
            452-552             AC111082.23        100987-101087       c
            553-673             AC111082.23        96450-96570         c
            674-861             AC111082.23        92203-92390         c
            862-940             AC111082.23        88566-88644         c
            941-1035            AC111082.23        88122-88216         c
            1036-1140           AC111082.23        86759-86863         c
            1141-1177           AC111082.23        84634-84670         c
            1178-1240           AC111082.23        82880-82942         c
            1241-1406           AC111082.23        78712-78877         c
            1407-1549           AC111082.23        78230-78372         c
            1550-1662           AC111082.23        71016-71128         c
            1663-1764           AC111082.23        70199-70300         c
            1765-1940           AC111082.23        67445-67620         c
            1941-2004           AC111082.23        60880-60943         c
            2005-5423           AC111082.23        55236-58654         c
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 19.67 cM"
     gene            1..5423
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /note="CD2-associated protein"
                     /db_xref="GeneID:12488"
                     /db_xref="MGI:MGI:1330281"
     exon            1..136
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..2046
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /note="isoform 1 is encoded by transcript variant 1; SH3
                     domain-containing adaptor protein METS-1;
                     mesenchyme-to-epithelium transition protein with SH3
                     domains 1; SH3 domain-containing adapter protein;
                     mesenchyme to epithelium transition protein, SH3 domains"
                     /codon_start=1
                     /product="CD2-associated protein isoform 1"
                     /protein_id="NP_033977.3"
                     /db_xref="CCDS:CCDS50114.1"
                     /db_xref="GeneID:12488"
                     /db_xref="MGI:MGI:1330281"
                     /translation="MVDYIVEYDYDAVHDDELTIRVGEIIRNVKKLQEEGWLEGELNG
                     RRGMFPDNFVKEIKRETEPKDDNLPIKRERQGNVASLVQRISTYGLPAGGIQPHPQTK
                     AIKKKTKKRQCKVLFDYSPQNEDELELIVGDVIDVIEEVEEGWWSGTLNNKLGLFPSN
                     FVKELESTEDGETHNAQEESEVPLTGPTSPLPSPGNGSEPAPGSVAQPKKIRGIGFGD
                     IFKEGSVKLRTRTSSSETEEKKTEKPLILQPLGSRTQNVEVTKPDVDGKIKAKEYCRT
                     LFPYTGTNEDELTFREGEIIHLISKETGEAGWWKGELNGKEGVFPDNFAVQISELDKD
                     FPKPKKPPPPAKGPAPKPDLSAAEKKAFPLKAEEKDEKSLLEQKPSKPAAPQVPPKKP
                     TAPTKASNLLRSPGAVYPKRPEKPVPPPPPAAKINGEVSIISSKIDTEPVSKPKLDPE
                     QLPVRPKSVDLDAFVARNSKETDDVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGG
                     HSPTQSPEKTLKLPKEDDSGNLKPLEFKKDASYSSKSSLSTPSSASKVNTAAFLTPLE
                     LKAKAEADDGKRNSVDELRAQIIELLCIVDALKKDHGKELEKLRKELEEEKAMRSNLE
                     VEIAKLKKAVLLS"
     exon            137..297
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            298..451
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            452..552
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            553..673
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            674..861
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            862..940
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1035
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1140
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1177
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1240
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1406
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1549
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            1550..1662
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1764
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..1940
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            1941..2004
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     exon            2005..5423
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5159..5164
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /note="hexamer: AATAAA"
     polyA_site      5181
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /note="major polyA site"
     regulatory      5402..5407
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /note="hexamer: AATAAA"
     polyA_site      5423
                     /gene="Cd2ap"
                     /gene_synonym="METS-1; Mets1"
                     /note="major polyA site"
ORIGIN      
        1 ggggctgcgg ccgaggccgg gcgtgaggag cggccaagcg cgccgcgggc ggatggagtc
       61 gcttccccgc cgcgcctgag ccggtgcaca gaaggggccg cggggccgcg ctctccccgc
      121 cagagccgca gcatggttga ctatattgtg gaatatgact atgatgctgt acatgatgat
      181 gagttaacta tccgagttgg ggaaatcatc agaaatgtga aaaaactaca ggaagaagga
      241 tggctagaag gagaactaaa tgggagacga gggatgttcc ctgacaattt tgttaaggaa
      301 attaagcgag agacagaacc caaggatgac aatttgccca tcaaacggga aaggcaagga
      361 aatgtagcaa gtcttgtaca gcgaatcagc acttatggac ttccagctgg aggaattcag
      421 ccacatccac aaaccaaagc tattaagaag aagacaaaaa agcgtcagtg taaagttctt
      481 tttgactact ctccacaaaa tgaggacgaa ctggaattga tcgtggggga tgtcattgac
      541 gtcattgaag aggtagaaga aggctggtgg agtggaaccc tgaacaataa gttgggactg
      601 tttccctcaa actttgtgaa agaattagag tccacagagg atggtgaaac tcacaacgct
      661 caggaggaat cagaagttcc tttgactggc cctacctcac cgttgccatc tccggggaat
      721 gggagtgaac ctgctcccgg atcagttgca cagccaaaga aaatccgagg aattggattt
      781 ggagacatct ttaaagaagg ctctgtgaaa cttcggacaa gaacttccag tagtgaaaca
      841 gaggagaaaa aaacagaaaa gcctttaatc ctacagcctc tgggatctag aactcagaat
      901 gtggaggtaa caaaaccaga cgttgatggc aagattaaag ctaaggaata ttgtagaaca
      961 ttatttccct atacaggaac caatgaagat gaacttacat ttagagaagg ggagattatc
     1021 catttgataa gtaaggagac tggagaagca ggctggtgga agggtgaact gaacggtaaa
     1081 gaaggagtgt ttccagataa ctttgctgtt cagataagtg aactagataa agactttcca
     1141 aaaccaaaga aaccacctcc tcctgcaaaa ggtccagctc caaagcctga cctgtcagct
     1201 gcagagaaga aagctttccc tctaaaggct gaagaaaaag atgaaaaatc actgttagaa
     1261 cagaaacctt ctaaaccagc tgctcctcaa gtcccgccca aaaagcccac tgcacccacc
     1321 aaagccagta acttactgag gtcgcctgga gcagtgtacc caaagcggcc agaaaagcca
     1381 gtccctccgc cacctccagc agccaaaatt aatggagaag tttctataat ttcttcaaag
     1441 attgatactg agccagtatc aaaaccaaag ctagatcctg aacaattgcc agttagacca
     1501 aaatcagtag accttgatgc ctttgtagcc aggaactcaa aagaaacaga tgatgtaaat
     1561 tttgatgaca tagcttcctc agagaacttg ttacatctca ctgctaatag acccaagatg
     1621 cctggaagac gattgcctgg ccgcttcaac ggtggacatt ctccaaccca aagcccagaa
     1681 aaaaccttga agctaccaaa agaagatgat agtggcaacc taaagccttt ggagtttaaa
     1741 aaggatgcaa gctactcttc aaagtcatct ctttcaacac cttcaagtgc ttctaaagta
     1801 aatacagctg ctttcctaac tccgttagaa ctcaaagcta aagcagaagc agatgatggg
     1861 aaaagaaatt ccgtggacga acttagagcc cagattattg aattgttgtg tattgtagat
     1921 gcactgaaaa aggatcatgg gaaagaactg gaaaagctac gcaaagaatt agaagaagag
     1981 aaggccatgc gaagtaatct agaggtggaa atagcaaagc tgaagaaagc tgttctgttg
     2041 tcttgaggtg gtgtggaccc agtgttccta atgattcatg aacttaactg acttgttaaa
     2101 gacagcagaa atggtggtgt gatgaagaaa taagaggaca taaactgtga catctaccga
     2161 aaagttgggg gtgacaggtt ttttttatat attttgtttt gccaatatga aaaaaaagaa
     2221 gaggccttat ttcttactgt gctgggattg caaacatttc tattgtttgc ttaaatacta
     2281 ctgaatctgt ataaaaagga aagaaagttc ataatagttt ttttattgaa acttgtatta
     2341 tttttaaaga gttctatact ataaatatcg taatatcttt acaaataagc tgtaagataa
     2401 actatttgag aaggacgggt tagctttata gtagagtcac tgctttcctg tactgcatag
     2461 ggcatgaact tgtatacata cactacacca cacacacaca cacacacaca cacacacaca
     2521 cacacacaca gacactttcc ttttagcttc ttacccagga ttacactgct gtgcctgttt
     2581 gtctgcagtg ctgtttgtac attgggtgat aaaataggag ttcctagtta ctaggttcct
     2641 cacccatgca tatttaatca ttaatgaaat aataacaatc caaattctag agtgttctat
     2701 aatgctcact ataggattca ttaaaggaag ctatttaaat tgtactggat taatcttgag
     2761 aaaataggag ctttgttcta aattcatgtg gcctaagaaa tctaaaactg tcatgctaat
     2821 tcttatttgt gcctagacac cgcagcacat gtggaatatg taaacaccag ccatattgtt
     2881 atattcttat gctcattcta cagtcttaaa tattactcat ttcattatct tgtattcatt
     2941 gtacccttcc tttaattttc actaatgata atcttaggaa acaaacctaa acccagatat
     3001 tttcagaatt ccagaatgag gttctatatt ggaaagctat tgtgtaaaca ctcaggtttt
     3061 acaggcataa tttatcgaat agaacaaact aagtcctaga gatattttgg actgttactt
     3121 aggtaaagtg aaaaagagta ttagtaattg accatattga agatcattcc acaatggatg
     3181 ttgagccatt tatttagcat cacaatcaac agttgaacat cttagactgg ctcagccact
     3241 atcagggcgg tatggctaca gaccaagtga acataaaaat gttgtatctg tagggcgaca
     3301 tgtttaaaaa cacaaaggtt aattttaaaa agtgaaatgt ttttatttgt taaaaaaaag
     3361 ttaggtttta agctttactt tagaaaatga atgtcagatt gtaaattatc atgcttaaaa
     3421 aaacaataca ttttctttac attttaatct taaaatagaa aaaaaattaa agtcagattt
     3481 cttgtgttat ttcaagtaaa atagagctga agtagaaaat aatagaatgc cagcacaagg
     3541 ttacacttga ttagattctg gggagagcta attgtacata gagtgtacat tgtaggtgtt
     3601 ttgacacagg aacttatata tcccaggctg gacttaacct tgtgatatag caaaattgtc
     3661 ttaaacttcc ggtttccctg cctctgcctc ccaagtgcta ggatgacagg catgcccagt
     3721 gtgcctagcc tttttaacca tttcaactct gagctcatct aatgagaggt tactgatgac
     3781 caaacttctc cattttattt ttaagtgatt agtcaaatga agtatttgct tcttattaat
     3841 gtaatgacag tcctcagatg acagtggcct taccctaagt atcatctcct acttccccca
     3901 cattttagaa atgatctcct gcattcccaa agggaggttt gtctccaccc acactgctgg
     3961 gagggaaagg tctaataaca gtaaaaagta acatacacag tcaacagtag gggagtgact
     4021 atgctttctg gacaaatagg taacccatag atactagtta aaatgtaaga aattagaaaa
     4081 gcagataact aacagaaaag tgagtttatg tgttataaac acaaataaga aaaacagaag
     4141 cacagcccct tattgtatac taagttttcc tttgcagttt ggctccttcc tgacccactg
     4201 tgtcacaacc tgacaggcga tctcagcccc aggagcacat gtagtttaat gcttgtatgc
     4261 tatttaaagg tccatttgag ccacgtgtag tgatgacatg cctgtaactt ggagatttgg
     4321 ggagagaaac catgagtttg aggccagcct gtgctgtata ggggccctat atccacaaga
     4381 aaggaaacag cgttcctttg gtggcggtgg gtatttttag tccacacaat cagagtagcc
     4441 atgagtcatc cctacacact tttgccatct cagtcagaat agagaagtca tactgtctgt
     4501 tttctatatg tctattttat gccctttaca aaatagttca gaaaaaaaac caaacaagct
     4561 tgaacttaat taaagggaaa aataattcct ttaaaagagt aatttgccag tatttgccct
     4621 tgatatggga acttttacta tttctaaagt taaagtcaga aaatgattaa taacaacaac
     4681 aactttactt agaggtaaat ccctcttccc ccaatattag tataaaagac agcattgaat
     4741 ctgtcattgc caaattatta gtgaatgtat agttcagatt tgctagtctt tgaaacacag
     4801 cactcctaag tttctaacag gattccaatg ttattttggt gtgtttgtga ccactaagct
     4861 tgcttcttac tataaaggta ttacctctat acaactgtac aggacttcac agaaaatcag
     4921 aagatgcaga gagagtgggc ccggtcttaa cagaggactc cttacactcc taactgtgat
     4981 cacagacaag aaataccttc cgaagtgctc tgtagatttt tttgatgaat gttttatgct
     5041 tgcatttaaa cttgaaatgt atgagcagaa tgagacaatc tttacaatca gaattgagaa
     5101 gtgttacaat tgaatggcct tgtgctgtag caataaaatg accaagtgca atgactttaa
     5161 taaaatcatc tttcaaacgt tgctgctatt aatattttta gttaacaaaa ttctgtcgtt
     5221 tagcctacct tgccttctgt ttgttaactg ttatgtgata cagttgatgt tttgacaatc
     5281 taacattcca aagtccacat atatatctta aaagtcttag aatagtatat tcattttttt
     5341 ttacaggtgc tgttaaaaaa ggaaatgaaa gcatgctttt ccctcaaaaa gaaaaattaa
     5401 taataaaaga gaactgtgct gcc
//
