LOCUS       NM_018865               4929 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Mus musculus cellular communication network factor 4 (Ccn4),
            transcript variant 1, mRNA.
ACCESSION   NM_018865
VERSION     NM_018865.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Dropmann,A., Alex,S., Schorn,K., Tong,C., Caccamo,T., Godoy,P.,
            Ilkavets,I., Liebe,R., Gonzalez,D., Hengstler,J.G., Piiper,A.,
            Quagliata,L., Matter,M.S., Waidmann,O., Finkelmeier,F., Feng,T.,
            Weiss,T.S., Rahbari,N., Birgin,E., Rasbach,E., Roessler,S.,
            Breuhahn,K., Toth,M., Ebert,M.P., Dooley,S., Hammad,S. and
            Meindl-Beinker,N.M.
  TITLE     The TGF-beta1 target WISP1 is highly expressed in liver cirrhosis
            and cirrhotic HCC microenvironment and involved in pro- and
            anti-tumorigenic effects
  JOURNAL   Biochem Biophys Res Commun 732, 150409 (2024)
   PUBMED   39033550
  REMARK    GeneRIF: The TGF-beta1 target WISP1 is highly expressed in liver
            cirrhosis and cirrhotic HCC microenvironment and involved in pro-
            and anti-tumorigenic effects.
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Li,Z., Williams,H., Jackson,M.L., Johnson,J.L. and George,S.J.
  TITLE     WISP-1 Regulates Cardiac Fibrosis by Promoting Cardiac Fibroblasts'
            Activation and Collagen Processing
  JOURNAL   Cells 13 (11), 989 (2024)
   PUBMED   38891121
  REMARK    GeneRIF: WISP-1 Regulates Cardiac Fibrosis by Promoting Cardiac
            Fibroblasts' Activation and Collagen Processing.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4929)
  AUTHORS   Green,L.C., Slone,S., Anthony,S.R., Guarnieri,A.R., Parkins,S.,
            Shearer,S.M., Nieman,M.L., Roy,S., Aube,J., Wu,X., Xu,L.,
            Kanisicak,O. and Tranter,M.
  TITLE     HuR-dependent expression of Wisp1 is necessary for TGFbeta-induced
            cardiac myofibroblast activity
  JOURNAL   J Mol Cell Cardiol 174, 38-46 (2023)
   PUBMED   36372279
  REMARK    GeneRIF: HuR-dependent expression of Wisp1 is necessary for
            TGFbeta-induced cardiac myofibroblast activity.
REFERENCE   4  (bases 1 to 4929)
  AUTHORS   van den Bosch,M.H.J. and Blaney Davidson,E.N.
  TITLE     Analysis of CCN4/WISP1 Effects on Joint Tissues Using Gain- and
            Loss-of-Function Approaches
  JOURNAL   Methods Mol Biol 2582, 369-390 (2023)
   PUBMED   36370364
  REMARK    GeneRIF: Analysis of CCN4/WISP1 Effects on Joint Tissues Using
            Gain- and Loss-of-Function Approaches.
REFERENCE   5  (bases 1 to 4929)
  AUTHORS   Liu,D., Zhang,M., Tian,J., Gao,M., Liu,M., Fu,X., Jin,T., Pan,J.,
            Chen,F. and An,F.
  TITLE     WNT1-inducible signalling pathway protein 1 stabilizes
            atherosclerotic plaques in apolipoprotein-E-deficient mice via the
            focal adhesion kinase/mitogen-activated extracellular
            signal-regulated kinase/extracellular signal-regulated kinase
            pathway
  JOURNAL   J Hypertens 40 (9), 1666-1681 (2022)
   PUBMED   35881419
  REMARK    GeneRIF: WNT1-inducible signalling pathway protein 1 stabilizes
            atherosclerotic plaques in apolipoprotein-E-deficient mice via the
            focal adhesion kinase/mitogen-activated extracellular
            signal-regulated kinase/extracellular signal-regulated kinase
            pathway.
REFERENCE   6  (bases 1 to 4929)
  AUTHORS   Yoshida,S., Mears,A.J., Friedman,J.S., Carter,T., He,S., Oh,E.,
            Jing,Y., Farjo,R., Fleury,G., Barlow,C., Hero,A.O. and Swaroop,A.
  TITLE     Expression profiling of the developing and mature Nrl-/- mouse
            retina: identification of retinal disease candidates and
            transcriptional regulatory targets of Nrl
  JOURNAL   Hum Mol Genet 13 (14), 1487-1503 (2004)
   PUBMED   15163632
REFERENCE   7  (bases 1 to 4929)
  AUTHORS   Sleeman,M.A., Murison,J.G., Strachan,L., Kumble,K., Glenn,M.P.,
            McGrath,A., Grierson,A., Havukkala,I., Tan,P.L. and Watson,J.D.
  TITLE     Gene expression in rat dermal papilla cells: analysis of 2529 ESTs
  JOURNAL   Genomics 69 (2), 214-224 (2000)
   PUBMED   11031104
REFERENCE   8  (bases 1 to 4929)
  AUTHORS   Pennica,D., Swanson,T.A., Welsh,J.W., Roy,M.A., Lawrence,D.A.,
            Lee,J., Brush,J., Taneyhill,L.A., Deuel,B., Lew,M., Watanabe,C.,
            Cohen,R.L., Melhem,M.F., Finley,G.G., Quirke,P., Goddard,A.D.,
            Hillan,K.J., Gurney,A.L., Botstein,D. and Levine,A.J.
  TITLE     WISP genes are members of the connective tissue growth factor
            family that are up-regulated in wnt-1-transformed cells and
            aberrantly expressed in human colon tumors
  JOURNAL   Proc Natl Acad Sci U S A 95 (25), 14717-14722 (1998)
   PUBMED   9843955
REFERENCE   9  (bases 1 to 4929)
  AUTHORS   Hashimoto,Y., Shindo-Okada,N., Tani,M., Nagamachi,Y., Takeuchi,K.,
            Shiroishi,T., Toma,H. and Yokota,J.
  TITLE     Expression of the Elm1 gene, a novel gene of the CCN (connective
            tissue growth factor, Cyr61/Cef10, and neuroblastoma overexpressed
            gene) family, suppresses In vivo tumor growth and metastasis of
            K-1735 murine melanoma cells
  JOURNAL   J Exp Med 187 (3), 289-296 (1998)
   PUBMED   9449709
REFERENCE   10 (bases 1 to 4929)
  AUTHORS   Hashimoto,Y., Shindo-Okada,N., Tani,M., Takeuchi,K., Toma,H. and
            Yokota,J.
  TITLE     Identification of genes differentially expressed in association
            with metastatic potential of K-1735 murine melanoma by messenger
            RNA differential display
  JOURNAL   Cancer Res 56 (22), 5266-5271 (1996)
   PUBMED   8912867
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC107859.7.
            
            On Sep 2, 2022 this sequence version replaced NM_018865.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB004873.1, SRR13948564.340630.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-147               AC107859.7         154192-154338
            148-427             AC107859.7         169105-169384
            428-688             AC107859.7         175533-175793
            689-882             AC107859.7         179981-180174
            883-4929            AC107859.7         181859-185905
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 29.3 cM"
     gene            1..4929
                     /gene="Ccn4"
                     /gene_synonym="Elm1; Wisp1"
                     /note="cellular communication network factor 4"
                     /db_xref="GeneID:22402"
                     /db_xref="MGI:MGI:1197008"
     exon            1..147
                     /gene="Ccn4"
                     /gene_synonym="Elm1; Wisp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..1182
                     /gene="Ccn4"
                     /gene_synonym="Elm1; Wisp1"
                     /note="WNT1 inducible signaling pathway protein 1; CCN
                     family member 4"
                     /codon_start=1
                     /product="CCN family member 4 precursor"
                     /protein_id="NP_061353.1"
                     /db_xref="CCDS:CCDS27510.1"
                     /db_xref="GeneID:22402"
                     /db_xref="MGI:MGI:1197008"
                     /translation="MRWLLPWTLAAVAVLRVGNILATALSPTPTTMTFTPAPLEETTT
                     RPEFCKWPCECPQSPPRCPLGVSLITDGCECCKICAQQLGDNCTEAAICDPHRGLYCD
                     YSGDRPRYAIGVCAQVVGVGCVLDGVRYTNGESFQPNCRYNCTCIDGTVGCTPLCLSP
                     RPPRLWCRQPRHVRVPGQCCEQWVCDDDARRPRQTALLDTRAFAASGAVEQRYENCIA
                     YTSPWSPCSTTCGLGISTRISNVNARCWPEQESRLCNLRPCDVDIQLHIKAGKKCLAV
                     YQPEEATNFTLAGCVSTRTYRPKYCGVCTDNRCCIPYKSKTISVDFQCPEGPGFSRQV
                     LWINACFCNLSCRNPNDIFADLESYPDFEEIAN"
     exon            148..427
                     /gene="Ccn4"
                     /gene_synonym="Elm1; Wisp1"
                     /inference="alignment:Splign:2.1.0"
     exon            428..688
                     /gene="Ccn4"
                     /gene_synonym="Elm1; Wisp1"
                     /inference="alignment:Splign:2.1.0"
     exon            689..882
                     /gene="Ccn4"
                     /gene_synonym="Elm1; Wisp1"
                     /inference="alignment:Splign:2.1.0"
     exon            883..4929
                     /gene="Ccn4"
                     /gene_synonym="Elm1; Wisp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4908..4913
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ccn4"
                     /gene_synonym="Elm1; Wisp1"
                     /note="hexamer: AATAAA"
     polyA_site      4929
                     /gene="Ccn4"
                     /gene_synonym="Elm1; Wisp1"
                     /note="major polyA site"
ORIGIN      
        1 agtcctggcc cagctccctg gagaggcatc cgcatcctct gggctgagcc gtagctcctg
       61 tgacgctgac ttccaggcat gaggtggctc ctgccctgga cgctggcagc cgtggcagtc
      121 ctgagggtgg gcaacatcct ggccacggcc ctctctccaa cccccacaac aatgaccttc
      181 accccagcac cactagagga aacgactaca cgccccgaat tctgcaagtg gccatgtgag
      241 tgcccacaat ccccacctcg ctgcccactg ggcgtcagcc taatcacaga tggctgtgaa
      301 tgctgtaaga tatgtgccca gcagcttggg gacaactgca cagaggctgc catctgtgac
      361 ccacaccggg gcctctactg cgattacagt ggggatcgcc cgaggtacgc aataggagtg
      421 tgtgcacagg tggtcggtgt gggctgtgtc ctggatggcg tacgctacac caatggcgag
      481 tccttccaac ccaactgcag gtacaactgt acctgcattg atggcacggt gggctgcaca
      541 ccgctgtgcc taagccccag gcccccacgc ctctggtgcc gccagccccg gcacgtgaga
      601 gtccctggcc agtgctgtga gcagtgggtg tgtgatgatg acgcaaggag accacgccag
      661 actgcactgt tggacaccag agcctttgca gcgtcaggcg ccgtggagca acggtatgag
      721 aactgcatag cctacactag tccctggagc ccctgctcta ccacctgtgg cctaggtatc
      781 tccactcgga tctctaacgt caatgcccgg tgctggccag agcaggaaag tcgcctctgc
      841 aacctgcggc catgtgatgt ggacatccaa ctacacatca aggcagggaa gaaatgcctg
      901 gctgtgtacc agccagagga ggccacgaac ttcactctcg caggctgtgt cagcacacgc
      961 acctaccgac ccaagtactg cggagtctgt actgacaata ggtgttgcat cccctacaag
     1021 tccaagacca tcagtgtgga tttccagtgt ccagaggggc caggtttctc ccggcaggtc
     1081 ctatggatta atgcttgctt ctgcaacctg agctgcagga atcctaacga tatctttgct
     1141 gacttggaat cttaccctga cttcgaagag attgccaatt aggtgggtgt gtggctcagg
     1201 gtaaagttcc atgctgcaaa gcagccagcc ctttgtggtc caggacttca caattgagcc
     1261 ttatttcatc tacttcctac tcgattctga attcccagtt tctgttcctg ttttgacaat
     1321 cgtaatggcc caggagagtg ctgctcaggc tcagacaatg ggttcctcct tggggacatt
     1381 ctacatcatt ccaaggaaaa cacatctctg actgttcaca atggaagcaa agcctggccc
     1441 agctagtctg gctccagcct gggcaagttg tcagaagttg tgatgggatt gtccaaggaa
     1501 aagcatcagc tgaagaacca gtatcatgaa gtccttcctc agatgccaag cctagggatg
     1561 ctgggatcct ttcagacaga tggatgggat tggggacaca ggaataagct attattttac
     1621 ccttgccaaa tgatactatc ctgggtattt ctgcctaaaa cataccaaaa gtgttcttgt
     1681 tccactgatc tgtatatcac aagtcaccaa acattttcca ggtgaggacc atagttgtgt
     1741 cattctgttt tgctattgaa aatcatttta aaaagaagga aaaaaaaaaa agaaaagaaa
     1801 agaaagtcat ttcattaact tgggcacttt ctcctctcac cccatattct ataaagggct
     1861 aaacttgggt tcttgttgat caggaatgta atttgagaag tcttactttt gcagggagat
     1921 ggtagccctc aattcatccc gtagggatgg gacaaggcca gccaatcttt caagccatag
     1981 ctgggcaggt cactgaatct gctgctggcc aagttcttag gacaattagc caaaatctgg
     2041 gcctctctct ccctagggtt catgggagtt ggtagggacc gtagagtgac ttgtctgttg
     2101 tctcaaaaag taagatggaa agatgttctc atggccctta gaagactctt ttgaagtcta
     2161 cgccagacct aacagaatat gtgcatcaaa caaacaagtg gatcaccctc ccatggcctg
     2221 ggtgacctct agcagtcacc cagtgactgt ggtaaggcca cagtagtccc tggacccagg
     2281 acaaatcttt tttgtttcag tgacctactt tacagcctca gtgtctatga agaaagttaa
     2341 ctcagttttc tcatctgaag acagaagtcg accagccatt cagaaatggg gatctattta
     2401 gtagtggtaa acgttaaaga gtttgtaagc taaagaaata ttttttttcc taagtgataa
     2461 tgaggttgtt acctatttta gaggtagaat tttctgtcat catcctttta ttgtgttcta
     2521 catactaccc attgttctca atatttgcca cacatttact cattgaaact tgctgataac
     2581 acagaggact tcccttaatg atttctgttc ttgacactgt gaaatatcaa ttcaaagagg
     2641 ctaataagtt caatcaaagt cacctatctg aagactcata gccagagaag attgccttca
     2701 tatttcccct ttgcttctgg accttgtaaa catgtcaggg caggaaagca tacagaagtc
     2761 agcagcttgc cctcttagta ttggctctct agcccctttc acgtaagagg agagtgtgtc
     2821 taggtactaa ggatccaacc taaactggac agaaaagggc atgtgcatta tcccctgtgg
     2881 ctcctcctgc cctggaatca gaactagctt acagcatgct tttatatgat gattcagttt
     2941 gccccacacc atacatatgg gcatatggga gaactataca cacactcata aatggatttg
     3001 taagtaggaa aggggacttg agatttcccc tccttcctgc aacttcccaa aggcctggcc
     3061 ttatgcaagg caaacagtgg tgcttaagat gtattttggg aagaaaatac tatatatata
     3121 tattcctatt tttccttaga agccttttct tccaggatct catcttaaca gtaagaaatc
     3181 ctctcttaag agaggcctag tattttgagt aaacatatgt tcatatgtgt acaagtactt
     3241 gtatgtaaat ataagttacc actgtgggtt attaattagg taaattttat gtggtatcat
     3301 atttgatttt cttctattgg aaaaatctgc cttaacagct aactctgtaa gaacttccat
     3361 aggagccata agctagggtc ttcccaggta tccatcctct tttgggaaac tgagctgagc
     3421 atcttcaacc aaaggagtta gggtgatcat tgggaatagg agaaagggat ggccagggta
     3481 gctccatcgt tatttagaaa cagacctggc atacagaacg accagaggaa ccaaccttct
     3541 ttgaaccaag ggaaaaagac ttggatgtaa tatatagaag ctttttctaa tagtcagaaa
     3601 cagactttaa ttgtatggcc tggttcaagg aaagttaaga atgtccatta tcgttaaaaa
     3661 caaaagtcca ttattgatag cttatttggt gctaagccct acggtgcatt ttgtccggct
     3721 atcacttagc agagccatgc tcagtacaca gtctttctct attcaataaa tagaaacgct
     3781 gaagctcaaa ggcactgagg agctgaggct cagaagcatg tttagtccac tctatcaggg
     3841 ggggaagaga tcttgacgga acctaaatga ctactattgg aaactcatat ttgaaagctt
     3901 tcagaagtcc cacccccacc ccatccccag atgcttagaa actaaagaag caatgaggat
     3961 gagctagcct tcagtgaaga ggttcactac tgcaccaaga gtgaatgtct taggtgtact
     4021 tagtcattgg acagggagac ctgagtgagt ttgtgaacct gcagcttact aaaccttaca
     4081 atgagcattt ggagagccaa gactgcttct cggcgcttta ctgacatggc ttgcttaatc
     4141 ttctcagtga gcccaagagt cagggcgtta ccactgccca ttttagggct gagaaagcaa
     4201 aatctccagg agttaagtga tttgctcaag tttttaacca accgaggcac tgcagataaa
     4261 ctccgaagcc cagtgtcatg taacatgccc atgccatctc tccggacacg cagcccattt
     4321 tcctgttcct aaaccaaagg ctcagagtca ccagaaccaa ctcacaggac agtgcagaaa
     4381 ttctaatgtc gagggtgatt agagactgat caaagaaagt aatttcaaat gatatgattg
     4441 tttgtaagca ccctagttaa ttctggacta catatgcata gagattgtga agaacattac
     4501 agcctgtgac tataacgttg acttctgtca tttcttttta aagacttgtt tttttttttt
     4561 tactcaaagg acccacagtg acagccctga atggttgaga agcattgatt agctgtgagt
     4621 cctgcatatg tatgtatgtg tgtgtgtgtg tgtgtatttg tatgtactta tctattttca
     4681 aactgtgatt gtgtatttaa atattcctcc tgccattttg taagtgatta cgcataaaga
     4741 aacacctttg aatgtcctaa taaaggagag ctagcccttg ggcggcctgt cacattttgc
     4801 cactttcctc atttttctca tgatctgtgt agcagggaat gtgtttgttc aaccatgatg
     4861 agttttcatt gttcaaattc tttgtttaca gcttttctcc ttaaagcaat aaatcatcag
     4921 caacagtaa
//
