LOCUS       NM_153518               1917 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus coiled-coil domain containing 65 (Ccdc65), transcript
            variant 1, mRNA.
ACCESSION   NM_153518
VERSION     NM_153518.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Zhang,Z., Xu,P., Hu,Z., Fu,Z., Deng,T., Deng,X., Peng,L., Xie,Y.,
            Long,L., Zheng,D., Shen,P., Zhang,M., Gong,B., Zhu,Z., Lin,J.,
            Chen,R., Liu,Z., Yang,H., Li,R. and Fang,W.
  TITLE     CCDC65, a Gene Knockout that leads to Early Death of Mice, acts as
            a potentially Novel Tumor Suppressor in Lung Adenocarcinoma
  JOURNAL   Int J Biol Sci 18 (10), 4171-4186 (2022)
   PUBMED   35844805
  REMARK    GeneRIF: CCDC65, a Gene Knockout that leads to Early Death of Mice,
            acts as a potentially Novel Tumor Suppressor in Lung
            Adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1917)
  AUTHORS   McClintock,T.S., Glasser,C.E., Bose,S.C. and Bergman,D.A.
  TITLE     Tissue expression patterns identify mouse cilia genes
  JOURNAL   Physiol Genomics 32 (2), 198-206 (2008)
   PUBMED   17971504
  REMARK    GeneRIF: Ccdc65 is most abundantly expressed in tissues rich in
            highly ciliated cells, such as olfactory sensory neurons, and is
            predicted to be important to cilia.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC156543.10.
            
            On Jul 12, 2018 this sequence version replaced NM_153518.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK077174.1, BC048073.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-343               AC156543.10        107464-107806
            344-511             AC156543.10        108325-108492
            512-681             AC156543.10        116694-116863
            682-820             AC156543.10        117982-118120
            821-1018            AC156543.10        119854-120051
            1019-1237           AC156543.10        120228-120446
            1238-1390           AC156543.10        121788-121940
            1391-1917           AC156543.10        122084-122610
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 54.65 cM"
     gene            1..1917
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
                     /note="coiled-coil domain containing 65"
                     /db_xref="GeneID:105833"
                     /db_xref="MGI:MGI:2146001"
     exon            1..343
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
                     /inference="alignment:Splign:2.1.0"
     CDS             203..1684
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
                     /note="isoform 1 is encoded by transcript variant 1;
                     coiled-coil domain-containing protein 65; testis
                     development protein NYD-SP28; dynein regulatory complex
                     subunit 2"
                     /codon_start=1
                     /product="dynein regulatory complex subunit 2 isoform 1"
                     /protein_id="NP_705738.1"
                     /db_xref="CCDS:CCDS27802.1"
                     /db_xref="GeneID:105833"
                     /db_xref="MGI:MGI:2146001"
                     /translation="MSKKGKKPKLPKAPLSEEDQLLIFQQKMLADEEAAKKKERLLTQ
                     FLKDKLAKEEHNSALNLNKINTQWRTILREVKTRELHQDIEILSQTFERVVDCKDSVI
                     KSLAKDLTEAEEQYAHALRSHLHNIDQLLTLQRRRLGLLEENYNMELEVLTKEFETER
                     KLIIDHHEKEMHYLQDVFMAMEQNYIDSEYESKLEFQSMWDDLKNKNLEEKHFLRLQL
                     ENIVEDLWRRFQDALKNYTDATEDRKIAFEYLKVKDEKSSKEIETQMKKIQKLQETIG
                     ILKGKIVAHSREGEWQNQCIRNNKELVHVQLRKLKVQRTQARTLSQENLVKLTLESNA
                     TLKALKKVVEKGEKILKLAEICRKFETEEEKVLPFYSSVLTPEEQEEAKLQNPEDITE
                     DLAKIMMDYAGMENFWKRYNKVKLEVLSLQHRRLQLLDISSKLREMLKQYLDGISVSD
                     EVLSHLNPLFVVNHRSNLPQLPPPSAQPVYNVIEAAHIASHIL"
     exon            344..511
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
                     /inference="alignment:Splign:2.1.0"
     exon            512..681
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
                     /inference="alignment:Splign:2.1.0"
     exon            682..820
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
                     /inference="alignment:Splign:2.1.0"
     exon            821..1018
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1237
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1390
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1917
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1879
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
                     /note="major polyA site"
     regulatory      1895..1900
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
                     /note="hexamer: AATAAA"
     polyA_site      1913
                     /gene="Ccdc65"
                     /gene_synonym="4933417K04Rik; NYD-SP28"
ORIGIN      
        1 aagatgcgtc tttctgttct ccagctggga gtcgggtgtc gcctgagcac tcttggcgtg
       61 gaggacagag gtctaaggcc ccattacctt agctccagaa ggtccctccc tgtctatggg
      121 aagtttttct ttctaattcc tcacaagtgg ctcgtagcag caggcagagg ctccttgccc
      181 ggacccagtc cgcagcccag ccatgtctaa aaaaggaaaa aagcccaagt tgcccaaggc
      241 acccctgtcc gaggaagatc agctgcttat atttcagcaa aagatgctgg ctgacgaaga
      301 ggcagccaag aagaaagaga gactcctcac ccagttcttg aaggacaagt tggccaaaga
      361 agaacacaac agcgctctaa accttaataa gattaacacg cagtggagaa ccatcctcag
      421 ggaggtgaag accagagagc ttcaccaaga catcgagatc ctcagccaga cctttgaacg
      481 cgtggtggac tgtaaggaca gcgtcatcaa gtctctggct aaggacttga ctgaagctga
      541 ggagcagtat gcccacgctc tgcgtagtca cttgcacaac attgaccagc tcttgaccct
      601 gcaacgacgc cggctcggcc tcctggagga aaattacaac atggagctcg aggtcctgac
      661 caaagagttt gagacggaaa ggaaattgat cattgaccat catgagaagg agatgcacta
      721 cctacaggac gtcttcatgg ccatggagca gaactacata gattccgagt atgagagcaa
      781 gctggagttc cagagcatgt gggatgacct taaaaacaag aatttagaag agaagcactt
      841 tcttcgccta caactggaga acatagtgga agatctgtgg cggaggttcc aggatgcact
      901 caagaattac accgacgcca cggaggaccg gaagatcgcc tttgagtacc tgaaggtgaa
      961 ggatgagaaa agctccaaag agattgagac gcagatgaaa aagatccaga agctgcagga
     1021 gaccataggt atcctgaaag ggaagatcgt agcccacagc cgcgaagggg aatggcagaa
     1081 tcagtgcatc cggaataaca aggagctggt ccacgtgcag cttcgaaaac ttaaggtcca
     1141 gaggacccag gctcggaccc tatctcagga gaacttagtc aaactcaccc tggaaagtaa
     1201 tgccaccctc aaggccctga agaaggtcgt ggaaaagggt gaaaagatcc ttaagcttgc
     1261 tgaaatatgt aggaaattcg aaaccgagga agaaaaggtg ctgcctttct actcttcagt
     1321 gctgactccc gaggagcagg aagaggccaa gctccagaac ccggaggata tcacagagga
     1381 ccttgccaag attatgatgg actacgcggg gatggagaac ttctggaaga gatacaacaa
     1441 ggtgaaactg gaggtcctga gtctccagca caggcggctg cagctgctgg atatcagtag
     1501 caagctgcgg gagatgctca aacagtactt ggatggcatc tccgtgagcg acgaagtgct
     1561 gagccacctc aacccgctct tcgtagtaaa ccacagaagc aacctacccc agctgccccc
     1621 accctcggcc cagcctgtct acaacgtcat cgaagccgcc cacattgcct cccatatcct
     1681 gtgaccggag aaggagactt ctttcctgac ccctgaaagg aggctttctc ctttttcctt
     1741 ttcttttgtg aaaccagcgt gctttgctct catgctcaga tggacaggca cactcagaca
     1801 gacagacaga cagacgctcc agctgcttac tacttaccac cactaccctt acagtgtaaa
     1861 cgatgacttc tttttttttt taaagagata aataaataaa agtaaaagaa aaacttc
//
