LOCUS       NM_177866               1730 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus cation channel, sperm associated 4 (Catsper4),
            transcript variant 1, mRNA.
ACCESSION   NM_177866 XM_986473
VERSION     NM_177866.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Lin,S., Ke,M., Zhang,Y., Yan,Z. and Wu,J.
  TITLE     Structure of a mammalian sperm cation channel complex
  JOURNAL   Nature 595 (7869), 746-750 (2021)
   PUBMED   34225353
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Chung,J.J., Navarro,B., Krapivinsky,G., Krapivinsky,L. and
            Clapham,D.E.
  TITLE     A novel gene required for male fertility and functional CATSPER
            channel formation in spermatozoa
  JOURNAL   Nat Commun 2, 153 (2011)
   PUBMED   21224844
REFERENCE   3  (bases 1 to 1730)
  AUTHORS   Rezaian,J., Movahedin,M. and Mowla,S.J.
  TITLE     CatSper genes expression, semen characteristics and histology of
            testes in the contusive spinal cord-injured mice model
  JOURNAL   Spinal Cord 47 (1), 76-81 (2009)
   PUBMED   18607386
  REMARK    GeneRIF: Data show that spinal cord injury doesn't significantly
            change the expression of CatSpers 4.
REFERENCE   4  (bases 1 to 1730)
  AUTHORS   Xia,J., Reigada,D., Mitchell,C.H. and Ren,D.
  TITLE     CATSPER channel-mediated Ca2+ entry into mouse sperm triggers a
            tail-to-head propagation
  JOURNAL   Biol Reprod 77 (3), 551-559 (2007)
   PUBMED   17554080
REFERENCE   5  (bases 1 to 1730)
  AUTHORS   Jin,J., Jin,N., Zheng,H., Ro,S., Tafolla,D., Sanders,K.M. and
            Yan,W.
  TITLE     Catsper3 and Catsper4 are essential for sperm hyperactivated
            motility and male fertility in the mouse
  JOURNAL   Biol Reprod 77 (1), 37-44 (2007)
   PUBMED   17344468
  REMARK    GeneRIF: Catsper3 and Catsper4 knockout male mice are completely
            infertile due to a quick loss of motility and a lack of
            hyperactivated motility under capacitating conditions.
REFERENCE   6  (bases 1 to 1730)
  AUTHORS   Qi,H., Moran,M.M., Navarro,B., Chong,J.A., Krapivinsky,G.,
            Krapivinsky,L., Kirichok,Y., Ramsey,I.S., Quill,T.A. and
            Clapham,D.E.
  TITLE     All four CatSper ion channel proteins are required for male
            fertility and sperm cell hyperactivated motility
  JOURNAL   Proc Natl Acad Sci U S A 104 (4), 1219-1223 (2007)
   PUBMED   17227845
REFERENCE   7  (bases 1 to 1730)
  AUTHORS   Jin,J.L., O'Doherty,A.M., Wang,S., Zheng,H., Sanders,K.M. and
            Yan,W.
  TITLE     Catsper3 and catsper4 encode two cation channel-like proteins
            exclusively expressed in the testis
  JOURNAL   Biol Reprod 73 (6), 1235-1242 (2005)
   PUBMED   16107607
  REMARK    GeneRIF: CATSPER4 is a cation-channel protein and has role in
            acrosome reaction and male fertility
REFERENCE   8  (bases 1 to 1730)
  AUTHORS   Lobley,A., Pierron,V., Reynolds,L., Allen,L. and Michalovich,D.
  TITLE     Identification of human and mouse CatSper3 and CatSper4 genes:
            characterisation of a common interaction domain and evidence for
            expression in testis
  JOURNAL   Reprod Biol Endocrinol 1, 53 (2003)
   PUBMED   12932298
  REMARK    GeneRIF: Catsper4 has a single channel-forming domain, a pore-loop
            with the consensus sequence TxDxW, & a coiled-coil protein-protein
            interaction domain in the C-terminal tail. CatSpers 1,2,3 & 4 may
            interact directly or indirectly to form a functional tetramer.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY104668.1, AK077145.1 and CR513858.1.
            
            On Jul 11, 2008 this sequence version replaced NM_177866.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK077145.1, BC108974.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-347               BY104668.1         2-348
            348-1725            AK077145.1         335-1712
            1726-1730           CR513858.1         1-5                 c
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 66.5 cM"
     gene            1..1730
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /note="cation channel, sperm associated 4"
                     /db_xref="GeneID:329954"
                     /db_xref="MGI:MGI:3043288"
     exon            1..112
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..1375
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /note="isoform 1 is encoded by transcript variant 1;
                     channel, sperm associated 4; cation channel of sperm 4;
                     cation channel sperm-associated protein 4"
                     /codon_start=1
                     /product="cation channel sperm-associated protein 4
                     isoform 1"
                     /protein_id="NP_808534.1"
                     /db_xref="CCDS:CCDS18766.1"
                     /db_xref="GeneID:329954"
                     /db_xref="MGI:MGI:3043288"
                     /translation="MSEKHKWWQQVENIDITHLGPKRKAYELLGRHEEQVLINRRDVM
                     EKKDAWDVQEFITQMYIKQLLRHPAFQLLLAFLLLSNAITIALRTNSYLGQKHYELFS
                     TIDDIVLTILICEVLLGWLNGFWIFWKDGWNILNFAIVFILFMGFFIKQLDMVAITYP
                     LRVLRLVHVCMAVEPLARIIKVILQSMPDLANVMALILFFMLVFSVFGVTLFGAFVPK
                     HFQNMGVALYTLFICITQDGWLDIYTDFQMDEREYAMEVGGAIYFAVFITLGAFIGLN
                     LFVVVVTTNLEQMMKTGEEEGHLNIKFTETEEDEDWTDELPLVHCTEARKDTSTVPKE
                     PLVGGPLSNLTEKTCDNFCLVLEAIQENLMEYKEIREELNMIVEEVSSIRFNQEQQNV
                     ILHKYTSKSATFLSEPPEGANKQDLITALVSREKVSDSNINMVNKHKFSH"
     exon            113..187
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /inference="alignment:Splign:2.1.0"
     exon            188..331
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /inference="alignment:Splign:2.1.0"
     exon            332..433
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /inference="alignment:Splign:2.1.0"
     exon            434..531
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /inference="alignment:Splign:2.1.0"
     exon            532..652
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /inference="alignment:Splign:2.1.0"
     exon            653..786
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /inference="alignment:Splign:2.1.0"
     exon            787..961
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1170
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1324
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1727
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1704..1709
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /note="hexamer: AATAAA"
     polyA_site      1727
                     /gene="Catsper4"
                     /gene_synonym="4933412L05"
                     /note="major polyA site"
ORIGIN      
        1 agcaaagaag cggcctggcc tctgaagaga aggggacaca gcaaagatgt ctgaaaaaca
       61 caagtggtgg cagcaggtgg agaacatcga catcacacac ctgggcccta agagaaaagc
      121 ctatgaactc ctgggtcggc atgaggagca agtgctcatc aaccgcagag atgtcatgga
      181 gaagaaggat gcctgggatg tacaggaatt catcactcaa atgtatatca agcagttgct
      241 ccgccatccg gccttccagc tgctgctggc ctttctgctg ctgtccaacg ctatcaccat
      301 tgcccttcgc accaactctt atctcggtca gaaacattac gagctattct cgaccataga
      361 tgacattgtg ttgacgatcc ttatctgcga ggttctgctt ggttggctta acggcttctg
      421 gattttctgg aaggatggct ggaatatcct caacttcgca attgtcttta tcttgtttat
      481 ggggttcttc ataaaacaac ttgacatggt tgccatcacc taccctctca gggtgctccg
      541 gctggtgcat gtgtgtatgg cggtggaacc cctggccaga atcatcaagg ttatcctgca
      601 gtcgatgcca gacttggcta atgtcatggc tctcatcctc ttcttcatgc tggtattctc
      661 tgtgtttggg gtcacgctct tcggtgcatt tgtgcccaag catttccaga acatgggggt
      721 tgccctgtac acgctcttca tctgcatcac tcaggatgga tggctggaca tctacactga
      781 cttccagatg gatgaaagag agtacgcgat ggaggtcggg ggcgccatct actttgccgt
      841 ctttatcacc ctcggtgcct tcattggtct caacttgttc gtcgtcgtgg tgaccacaaa
      901 cctggaacaa atgatgaaga ccggcgagga agagggacac ctgaacataa agtttactga
      961 gacagaagag gatgaggact ggaccgacga gctgccactg gtgcattgta cagaggcccg
     1021 caaggatact tccactgtcc ccaaggaacc actggttggg ggccccctga gtaacctcac
     1081 agaaaagacc tgcgataact tctgcttggt gcttgaagca atacaggaga acttgatgga
     1141 gtacaaagag atccgagagg aactcaacat gatcgtggag gaagtgtcct ccatccggtt
     1201 caaccaggag cagcaaaatg tgatcctaca caagtatacc tccaaaagcg ccaccttcct
     1261 aagcgagccc ccagaagggg ctaacaagca agacttgatc actgcgctgg tcagcaggga
     1321 aaaggtgtct gattctaaca taaacatggt taacaaacac aagttcagcc actgagaagg
     1381 cagtatggga actggagggc ctgcacacac tgggccagct gctgcatccc agactggagc
     1441 ccatccctct cccttacacc cgggcggcag aggccatggg gctggaaagg tggcagcatc
     1501 tgcagggctt atgcccagga gcctcaagtc cggaggagtc gggattctgg ctgtgagtgg
     1561 gcgtacagaa agttgtagcc aggctttggc ctaaagatga acatccctga cctctgtctg
     1621 catcagccag agctggatga ggcctgcctg ggcccaagca ccagaaagag aagagccagg
     1681 ccagtgagac cgtgtgcacc aaaaataaac ttttgtgtta taaccaaaaa
//
