LOCUS       NM_001252487            1352 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus cation channel, sperm associated 3 (Catsper3),
            transcript variant 1, mRNA.
ACCESSION   NM_001252487
VERSION     NM_001252487.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Lin,S., Ke,M., Zhang,Y., Yan,Z. and Wu,J.
  TITLE     Structure of a mammalian sperm cation channel complex
  JOURNAL   Nature 595 (7869), 746-750 (2021)
   PUBMED   34225353
REFERENCE   3  (bases 1 to 1352)
  AUTHORS   Bystroff,C.
  TITLE     Intramembranal disulfide cross-linking elucidates the
            super-quaternary structure of mammalian CatSpers
  JOURNAL   Reprod Biol 18 (1), 76-82 (2018)
   PUBMED   29371110
  REMARK    GeneRIF: Data suggest that Catsper1, Catsper2, Catsper3, and
            Catsper4 form protein multimer with hypothetical activities of
            flippase and disulfide isomerase.
REFERENCE   4  (bases 1 to 1352)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 1352)
  AUTHORS   Chung,J.J., Navarro,B., Krapivinsky,G., Krapivinsky,L. and
            Clapham,D.E.
  TITLE     A novel gene required for male fertility and functional CATSPER
            channel formation in spermatozoa
  JOURNAL   Nat Commun 2, 153 (2011)
   PUBMED   21224844
REFERENCE   6  (bases 1 to 1352)
  AUTHORS   Xia,J., Reigada,D., Mitchell,C.H. and Ren,D.
  TITLE     CATSPER channel-mediated Ca2+ entry into mouse sperm triggers a
            tail-to-head propagation
  JOURNAL   Biol Reprod 77 (3), 551-559 (2007)
   PUBMED   17554080
REFERENCE   7  (bases 1 to 1352)
  AUTHORS   Jin,J., Jin,N., Zheng,H., Ro,S., Tafolla,D., Sanders,K.M. and
            Yan,W.
  TITLE     Catsper3 and Catsper4 are essential for sperm hyperactivated
            motility and male fertility in the mouse
  JOURNAL   Biol Reprod 77 (1), 37-44 (2007)
   PUBMED   17344468
  REMARK    GeneRIF: Catsper3 and Catsper4 knockout male mice are completely
            infertile due to a quick loss of motility and a lack of
            hyperactivated motility under capacitating conditions.
REFERENCE   8  (bases 1 to 1352)
  AUTHORS   Qi,H., Moran,M.M., Navarro,B., Chong,J.A., Krapivinsky,G.,
            Krapivinsky,L., Kirichok,Y., Ramsey,I.S., Quill,T.A. and
            Clapham,D.E.
  TITLE     All four CatSper ion channel proteins are required for male
            fertility and sperm cell hyperactivated motility
  JOURNAL   Proc Natl Acad Sci U S A 104 (4), 1219-1223 (2007)
   PUBMED   17227845
REFERENCE   9  (bases 1 to 1352)
  AUTHORS   Jin,J.L., O'Doherty,A.M., Wang,S., Zheng,H., Sanders,K.M. and
            Yan,W.
  TITLE     Catsper3 and catsper4 encode two cation channel-like proteins
            exclusively expressed in the testis
  JOURNAL   Biol Reprod 73 (6), 1235-1242 (2005)
   PUBMED   16107607
  REMARK    GeneRIF: CATSPER3 is a cation-channel protein and has role in
            acrosome reaction and male fertility
REFERENCE   10 (bases 1 to 1352)
  AUTHORS   Lobley,A., Pierron,V., Reynolds,L., Allen,L. and Michalovich,D.
  TITLE     Identification of human and mouse CatSper3 and CatSper4 genes:
            characterisation of a common interaction domain and evidence for
            expression in testis
  JOURNAL   Reprod Biol Endocrinol 1, 53 (2003)
   PUBMED   12932298
  REMARK    GeneRIF: Catsper3 has a single channel-forming domain, a pore-loop
            with the consensus sequence TxDxW, & a coiled-coil protein-protein
            interaction domain in the C-terminal tail. CatSpers 1,2,3 & 4 may
            interact directly or indirectly to form a functional tetramer.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC129780.5.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY263400.1, HM179540.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-181               AC129780.5         73371-73551         c
            182-335             AC129780.5         71611-71764         c
            336-575             AC129780.5         59147-59386         c
            576-758             AC129780.5         53164-53346         c
            759-899             AC129780.5         52276-52416         c
            900-1019            AC129780.5         51774-51893         c
            1020-1174           AC129780.5         49956-50110         c
            1175-1352           AC129780.5         49130-49307         c
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 30.06 cM"
     gene            1..1352
                     /gene="Catsper3"
                     /gene_synonym="4921522D01Rik; CACRC"
                     /note="cation channel, sperm associated 3"
                     /db_xref="GeneID:76856"
                     /db_xref="MGI:MGI:1924106"
     exon            1..181
                     /gene="Catsper3"
                     /gene_synonym="4921522D01Rik; CACRC"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..1271
                     /gene="Catsper3"
                     /gene_synonym="4921522D01Rik; CACRC"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cation channel sperm-associated protein 3"
                     /codon_start=1
                     /product="cation channel sperm-associated protein 3
                     isoform 1"
                     /protein_id="NP_001239416.1"
                     /db_xref="CCDS:CCDS56885.1"
                     /db_xref="GeneID:76856"
                     /db_xref="MGI:MGI:1924106"
                     /translation="MSQHFHHNPVRVKSGSLFATASEALQARLSKIKRKDKECQAYFR
                     KVIKSTFFQIVMITTVTTNSFLLVLGTNYDIQFEFFRTFEVSELFFVSVYVCEFLMKV
                     YVDPITYWKDGYNILDVIILIILTIPYLLRKIKGNHSAYLHFADGIQSLRILKLISYS
                     RGIRTLIIAVGETVYTVASVLTLLFLLMFVFAILGFCLFGVTDRGDLENWGNLASAFF
                     TLFSLATVDGWTDLQEELDKRKFTVSRAFTILFILLASFIFLNMFVGVMIMHTEDSMK
                     KFERDLTLERNLAIMEEKQIILKRQQEEVNRLMNTQKSGSMNFIDMVEGFKKTLRHTD
                     PMVLDDFSTSLSFIDIYLVTLDNQDVIVSKLQELYCEIVNVLSLMLEDMPKESSSSLS
                     GLS"
     exon            182..335
                     /gene="Catsper3"
                     /gene_synonym="4921522D01Rik; CACRC"
                     /inference="alignment:Splign:2.1.0"
     exon            336..575
                     /gene="Catsper3"
                     /gene_synonym="4921522D01Rik; CACRC"
                     /inference="alignment:Splign:2.1.0"
     exon            576..758
                     /gene="Catsper3"
                     /gene_synonym="4921522D01Rik; CACRC"
                     /inference="alignment:Splign:2.1.0"
     exon            759..899
                     /gene="Catsper3"
                     /gene_synonym="4921522D01Rik; CACRC"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1019
                     /gene="Catsper3"
                     /gene_synonym="4921522D01Rik; CACRC"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1174
                     /gene="Catsper3"
                     /gene_synonym="4921522D01Rik; CACRC"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1352
                     /gene="Catsper3"
                     /gene_synonym="4921522D01Rik; CACRC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1320..1325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Catsper3"
                     /gene_synonym="4921522D01Rik; CACRC"
                     /note="hexamer: ATTAAA"
     polyA_site      1352
                     /gene="Catsper3"
                     /gene_synonym="4921522D01Rik; CACRC"
                     /note="major polyA site"
ORIGIN      
        1 gtagaattca ggtgccaagg accttaggca gaggtgttta tctgtatgat aaaagtgagg
       61 aaggaagaca gaaaatttgg aaaatgtccc aacattttca ccacaaccct gtacgagtca
      121 agtcgggctc actgtttgct acagcatcgg aagcattgca ggcaagactg agcaagatta
      181 agaggaagga taaggagtgc caggcttact tcaggaaggt tattaagagc actttcttcc
      241 agattgtgat gatcaccacg gtcaccacca actccttttt actggtcttg gggactaatt
      301 atgacataca attcgagttt ttcagaacct ttgaggtctc agagcttttc tttgtatctg
      361 tctatgtctg cgagttcctc atgaaggtct atgtggaccc cattacatac tggaaggatg
      421 gctataacat actggatgtg atcattctca tcattctcac cataccctat ctcctccgca
      481 aaatcaaggg gaatcattct gcatacctcc actttgctga tggcatccag tctctacgaa
      541 tcctcaagct tatctcctac agtaggggca tcaggacact catcatcgct gtgggggaga
      601 cggtctacac tgtggcctcg gtgctgacgc tgctcttcct cctcatgttt gtgttcgcga
      661 tcctgggatt ctgcctattt ggcgtgacgg acagaggcga cctggagaac tgggggaacc
      721 tggcttcagc tttctttact ctcttcagtt tggccacggt tgatggctgg actgacctgc
      781 aggaagagct ggacaagagg aagtttactg tgagccgggc gtttactatc ctcttcatct
      841 tgcttgcatc cttcatcttc ctcaacatgt ttgtgggtgt gatgatcatg cacacggagg
      901 attccatgaa aaagtttgag cgggatctga cgttggagag gaaccttgcg attatggagg
      961 agaagcaaat aatcctgaaa cgccagcaag aggaggtcaa caggctgatg aacacacaga
     1021 aaagtggtag catgaacttc attgatatgg tggagggctt caagaagacc ctgcggcaca
     1081 cagaccccat ggttctggat gacttcagca ctagtctctc cttcattgat atctacttgg
     1141 tcactctgga caaccaagat gttattgtca gcaagcttca ggagctctac tgtgagattg
     1201 tgaacgtgct gagcctgatg ttggaagaca tgcccaagga gagctcgtcc agcctctcgg
     1261 gactaagtta gaggtagccg tagctgggag ggcgtggcct cgaagactat gacagtccca
     1321 ttaaacacaa gcctccaggg gtggtttctc aa
//
