LOCUS       NM_153075               2247 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus cation channel, sperm associated 2 (Catsper2), mRNA.
ACCESSION   NM_153075
VERSION     NM_153075.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Contreras-Marciales,A.D.P., Lopez-Guzman,S.F., Benitez-Hess,M.L.,
            Oviedo,N. and Hernandez-Sanchez,J.
  TITLE     Characterization of the promoter region of the murine Catsper2 gene
  JOURNAL   FEBS Open Bio 12 (12), 2236-2249 (2022)
   PUBMED   36345591
  REMARK    GeneRIF: Characterization of the promoter region of the murine
            Catsper2 gene.
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Lin,S., Ke,M., Zhang,Y., Yan,Z. and Wu,J.
  TITLE     Structure of a mammalian sperm cation channel complex
  JOURNAL   Nature 595 (7869), 746-750 (2021)
   PUBMED   34225353
REFERENCE   3  (bases 1 to 2247)
  AUTHORS   Horiuchi,K., Perez-Cerezales,S., Papasaikas,P., Ramos-Ibeas,P.,
            Lopez-Cardona,A.P., Laguna-Barraza,R., Fonseca Balvis,N.,
            Pericuesta,E., Fernandez-Gonzalez,R., Planells,B., Viera,A.,
            Suja,J.A., Ross,P.J., Alen,F., Orio,L., Rodriguez de Fonseca,F.,
            Pintado,B., Valcarcel,J. and Gutierrez-Adan,A.
  TITLE     Impaired Spermatogenesis, Muscle, and Erythrocyte Function in U12
            Intron Splicing-Defective Zrsr1 Mutant Mice
  JOURNAL   Cell Rep 23 (1), 143-155 (2018)
   PUBMED   29617656
REFERENCE   4  (bases 1 to 2247)
  AUTHORS   Bystroff,C.
  TITLE     Intramembranal disulfide cross-linking elucidates the
            super-quaternary structure of mammalian CatSpers
  JOURNAL   Reprod Biol 18 (1), 76-82 (2018)
   PUBMED   29371110
  REMARK    GeneRIF: Data suggest that Catsper1, Catsper2, Catsper3, and
            Catsper4 form protein multimer with hypothetical activities of
            flippase and disulfide isomerase.
REFERENCE   5  (bases 1 to 2247)
  AUTHORS   Lishko,P.V., Botchkina,I.L. and Kirichok,Y.
  TITLE     Progesterone activates the principal Ca2+ channel of human sperm
  JOURNAL   Nature 471 (7338), 387-391 (2011)
   PUBMED   21412339
REFERENCE   6  (bases 1 to 2247)
  AUTHORS   Marquez,B., Ignotz,G. and Suarez,S.S.
  TITLE     Contributions of extracellular and intracellular Ca2+ to regulation
            of sperm motility: Release of intracellular stores can
            hyperactivate CatSper1 and CatSper2 null sperm
  JOURNAL   Dev Biol 303 (1), 214-221 (2007)
   PUBMED   17174296
REFERENCE   7  (bases 1 to 2247)
  AUTHORS   Qi,H., Moran,M.M., Navarro,B., Chong,J.A., Krapivinsky,G.,
            Krapivinsky,L., Kirichok,Y., Ramsey,I.S., Quill,T.A. and
            Clapham,D.E.
  TITLE     All four CatSper ion channel proteins are required for male
            fertility and sperm cell hyperactivated motility
  JOURNAL   Proc Natl Acad Sci U S A 104 (4), 1219-1223 (2007)
   PUBMED   17227845
REFERENCE   8  (bases 1 to 2247)
  AUTHORS   Carlson,A.E., Quill,T.A., Westenbroek,R.E., Schuh,S.M., Hille,B.
            and Babcock,D.F.
  TITLE     Identical phenotypes of CatSper1 and CatSper2 null sperm
  JOURNAL   J Biol Chem 280 (37), 32238-32244 (2005)
   PUBMED   16036917
  REMARK    GeneRIF: CatSper1 and CatSper2 null sperm show identical phenotypes
            in mice
REFERENCE   9  (bases 1 to 2247)
  AUTHORS   Quill,T.A., Sugden,S.A., Rossi,K.L., Doolittle,L.K., Hammer,R.E.
            and Garbers,D.L.
  TITLE     Hyperactivated sperm motility driven by CatSper2 is required for
            fertilization
  JOURNAL   Proc Natl Acad Sci U S A 100 (25), 14869-14874 (2003)
   PUBMED   14657366
REFERENCE   10 (bases 1 to 2247)
  AUTHORS   Quill,T.A., Ren,D., Clapham,D.E. and Garbers,D.L.
  TITLE     A voltage-gated ion channel expressed specifically in spermatozoa
  JOURNAL   Proc Natl Acad Sci U S A 98 (22), 12527-12531 (2001)
   PUBMED   11675491
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845466.4.
            
            On Jun 25, 2009 this sequence version replaced NM_153075.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF411816.1, BC132140.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-377               AL845466.4         120067-120443       c
            378-518             AL845466.4         118801-118941       c
            519-692             AL845466.4         117028-117201       c
            693-761             AL845466.4         116789-116857       c
            762-934             AL845466.4         113994-114166       c
            935-1090            AL845466.4         113358-113513       c
            1091-1215           AL845466.4         112980-113104       c
            1216-1394           AL845466.4         106695-106873       c
            1395-1494           AL845466.4         106388-106487       c
            1495-1518           AL845466.4         106097-106120       c
            1519-1946           AL845466.4         104112-104539       c
            1947-2114           AL845466.4         103604-103771       c
            2115-2247           AL845466.4         101006-101138       c
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.38 cM"
     gene            1..2247
                     /gene="Catsper2"
                     /note="cation channel, sperm associated 2"
                     /db_xref="GeneID:212670"
                     /db_xref="MGI:MGI:2387404"
     exon            1..377
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     exon            378..518
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     CDS             380..2146
                     /gene="Catsper2"
                     /codon_start=1
                     /product="cation channel sperm-associated protein 2"
                     /protein_id="NP_694715.2"
                     /db_xref="CCDS:CCDS38216.1"
                     /db_xref="GeneID:212670"
                     /db_xref="MGI:MGI:2387404"
                     /translation="MAQEQGHFQLLRADAIRSKLIDTFSLIEHLQGLSQAVPRHTLRE
                     ILDPSYQQKLMSGDQEQLVRFSIKPRRMGHITHSRRLLSRLRVRCSRMPPLSLWAGWV
                     LDSSVFSKFIISLIFLNTFVLMVEIELMESTNTALWPVKLALEVADWFILLSFIVEIL
                     LMWLASFSLFWKDAWNVFDFFVTLLSLLPELVVLLGVPAHSVWLQLLRVCRVLRSLKL
                     FARFRQIKVILLALVRALKSMTFLLMLLLIFFYIFAVTGVYFFREYSRSTIEGLEYNM
                     FFSDLLNSLVTVFILFTLDHWYAVLQDIWKVPESSRVFSSIYVILWLLLGSIIFRNII
                     VAMMVTNFQNIRSELSEEMSHLEVQYKADMFKQQIIQRRQHSESLRGTSLGKVSEDII
                     ETSDASDDDDDDDDDDDDDDDDDDKSDATESDGEKNENEESDSENSESENSESEKIDP
                     EKDYAKKSYPEKSHPEKSYPEKSHPEKSYPEKSHPKKSYDEQAEAEESKEKAYPVSHS
                     ISSHGSTAADTAFLENLDWETLVHENLPGLMDMDQDDRIVWPRDSLFRYFELLEKLQY
                     NLEERKKLQEFAVQALMSFEDK"
     exon            519..692
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     exon            693..761
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     exon            762..934
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1090
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1215
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1394
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1494
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1518
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1946
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2114
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2247
                     /gene="Catsper2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2229..2234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Catsper2"
                     /note="hexamer: AATAAA"
     polyA_site      2247
                     /gene="Catsper2"
                     /note="major polyA site"
ORIGIN      
        1 ttaggcgaac gcccgccggt ctggctgtgg cggaggcctg gacggggggt cggtgggaag
       61 tagggggctc agcgaagccg gagcggccct gagcgccacc cgctggggag gagtgaagct
      121 ctgggcgccg gcgcggaagg cggaggggtg aagtcagggc tgagtgggcg gaagtgaggc
      181 ccgcgaaagg gctggagggg agcttgagct gtctgtccgg ctccctccct ccggggcatt
      241 ggggcgcgca cgcacttgag cgcaccgcga cacgttttcc aaatgtcagg ctgttgcttt
      301 gtccaattat agggcgtagt aagtctacat cctgccgctg ccctacctcg tatcctgctt
      361 tgtcaggtcc acgtgagaca tggcacaaga acaaggacat ttccagctgc tcagagctga
      421 tgctatccgt tcaaagctca ttgacacttt ctcgctcata gagcatttgc agggcttgag
      481 ccaagccgta ccaaggcaca ctctccggga gatacttgat ccttcttacc agcagaaact
      541 catgtcagga gatcaggagc agctagtgcg cttctccata aagcctcggc gaatggggca
      601 catcacacac tcgcggcggt tgctgagcag gcttcgcgtg cggtgcagtc gaatgccccc
      661 tctttccttg tgggctggat gggtccttga tagttctgtc ttctcgaaat tcatcatctc
      721 cctcatcttt ctgaacacct ttgtgctgat ggttgaaata gaattgatgg aatccacaaa
      781 tactgctctg tggccagtga agctggcttt ggaggtggca gattggttca tcttgcttag
      841 cttcattgta gagatacttc taatgtggtt ggccagtttt tctctcttct ggaaggatgc
      901 ctggaatgtc tttgactttt ttgttacctt gttgtctctg cttcctgagt tagtagtgct
      961 gttaggagtc ccagcacact ctgtgtggct ccagctgctg agggtctgtc gggtgctgag
     1021 gtctctcaaa ctgtttgcac gattccgtca aattaaagtt attcttttgg ctctggtcag
     1081 ggccctgaag agcatgacgt tcctcttgat gttgctgctt atcttcttct acatttttgc
     1141 tgtgactggt gtctacttct tcagagaata ttcccgatca actatcgagg gcctggagta
     1201 caacatgttc ttctcggacc tactaaattc actggtgaca gtgttcatcc tcttcacctt
     1261 ggatcattgg tatgcagtac ttcaggatat ctggaaggtg ccagaatcta gccgtgtctt
     1321 tagcagcatc tatgttatcc tttggttgct gcttggctcc ataatctttc gaaatatcat
     1381 agtagccatg atggttacta actttcagaa tatcagaagt gagctgagtg aggagatgag
     1441 ccacctggag gttcagtata aagctgacat gttcaagcaa cagattatcc agaggagaca
     1501 gcactctgaa tcactaagag ggaccagtct tggaaaggtc tccgaagaca taatagaaac
     1561 ttctgatgct agtgatgatg atgacgatga cgacgatgat gacgacgacg atgatgatga
     1621 tgatgacaaa agcgatgcta ctgaaagcga tggcgagaaa aacgaaaacg aggaaagcga
     1681 tagtgagaat agtgagagtg agaatagcga gagcgagaaa attgatcctg agaaagacta
     1741 tgccaagaaa agctatcctg agaaaagcca tcctgagaaa agctatcctg agaaaagcca
     1801 tcctgagaaa agctatcctg agaaaagcca tcctaagaaa agctatgatg aacaagctga
     1861 agctgaagag tcaaaagaaa aagcctaccc agtttcccat tcaatctcgt cccatggctc
     1921 cactgcagcc gatactgctt tccttgaaaa cctggactgg gagacccttg tgcatgagaa
     1981 cctgcctggg ctaatggaca tggatcagga tgaccgcatt gtctggccca gagactcact
     2041 cttccgatat ttcgagttac tggaaaagct tcagtataac ctagaagagc gcaagaagtt
     2101 acaagaattt gcagtccagg ccctgatgag ttttgaagac aagtgaaggt acagtgggtg
     2161 acagctgtgt ccttggaccc agcaaaagct aatgaagaaa ataattggaa tttaaaatat
     2221 aaatatctaa taaatactgc taatgaa
//
