LOCUS       NM_009817               3925 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus calpastatin (Cast), transcript variant 2, mRNA.
ACCESSION   NM_009817 XM_903480
VERSION     NM_009817.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Williams,J.N., Irwin,M., Li,Y., Kambrath,A.V., Mattingly,B.T.,
            Patel,S., Kittaka,M., Collins,R.N., Clough,N.A., Doud,E.H.,
            Mosley,A.L., Bellido,T., Bruzzaniti,A., Plotkin,L.I.,
            Trinidad,J.C., Thompson,W.R., Bonewald,L.F. and Sankar,U.
  TITLE     Osteocyte-Derived CaMKK2 Regulates Osteoclasts and Bone Mass in a
            Sex-Dependent Manner through Secreted Calpastatin
  JOURNAL   Int J Mol Sci 24 (5), 4718 (2023)
   PUBMED   36902150
  REMARK    GeneRIF: Osteocyte-Derived CaMKK2 Regulates Osteoclasts and Bone
            Mass in a Sex-Dependent Manner through Secreted Calpastatin.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3925)
  AUTHORS   Hu,D., Sun,X., Magpusao,A., Fedorov,Y., Thompson,M., Wang,B.,
            Lundberg,K., Adams,D.J. and Qi,X.
  TITLE     Small-molecule suppression of calpastatin degradation reduces
            neuropathology in models of Huntington's disease
  JOURNAL   Nat Commun 12 (1), 5305 (2021)
   PUBMED   34489447
  REMARK    GeneRIF: Small-molecule suppression of calpastatin degradation
            reduces neuropathology in models of Huntington's disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3925)
  AUTHORS   Flores-Toro,J., Chun,S.K., Shin,J.K., Campbell,J.,
            Lichtenberger,M., Chapman,W., Zendejas,I., Behrns,K.,
            Leeuwenburgh,C. and Kim,J.S.
  TITLE     Critical Roles of Calpastatin in Ischemia/Reperfusion Injury in
            Aged Livers
  JOURNAL   Cells 10 (8), 1863 (2021)
   PUBMED   34440632
  REMARK    GeneRIF: Critical Roles of Calpastatin in Ischemia/Reperfusion
            Injury in Aged Livers.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3925)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   6  (bases 1 to 3925)
  AUTHORS   Li,S. and Goldberg,E.
  TITLE     A novel N-terminal domain directs membrane localization of mouse
            testis-specific calpastatin
  JOURNAL   Biol Reprod 63 (6), 1594-1600 (2000)
   PUBMED   11090425
REFERENCE   7  (bases 1 to 3925)
  AUTHORS   Takano,J., Watanabe,M., Hitomi,K. and Maki,M.
  TITLE     Four types of calpastatin isoforms with distinct amino-terminal
            sequences are specified by alternative first exons and
            differentially expressed in mouse tissues
  JOURNAL   J Biochem 128 (1), 83-92 (2000)
   PUBMED   10876161
REFERENCE   8  (bases 1 to 3925)
  AUTHORS   Takano,J., Kawamura,T., Murase,M., Hitomi,K. and Maki,M.
  TITLE     Structure of mouse calpastatin isoforms: implications of
            species-common and species-specific alternative splicing
  JOURNAL   Biochem Biophys Res Commun 260 (2), 339-345 (1999)
   PUBMED   10403772
REFERENCE   9  (bases 1 to 3925)
  AUTHORS   Li,J., Nixon,R., Messer,A., Berman,S. and Bursztajn,S.
  TITLE     Altered gene expression for calpain/calpastatin system in motor
            neuron degeneration (Mnd) mutant mouse brain and spinal cord
  JOURNAL   Brain Res Mol Brain Res 53 (1-2), 174-186 (1998)
   PUBMED   9473662
REFERENCE   10 (bases 1 to 3925)
  AUTHORS   Lee,W.J., Hatanaka,M. and Maki,M.
  TITLE     Multiple forms of rat calpastatin cDNA in the coding region of
            functionally unknown amino-terminal domain
  JOURNAL   Biochim Biophys Acta 1129 (2), 251-253 (1992)
   PUBMED   1730065
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CD550676.1, AK028843.1,
            AK083395.1 and AK037098.1.
            
            On Jul 26, 2014 this sequence version replaced NM_009817.3.
            
            Summary: This gene encodes an inhibitor of the calcium-dependent
            cysteine protease, calpain. This protein plays roles in multiple
            processes, including apoptosis, cell cycle regulation, and membrane
            fusion. Multiple protein isoforms exist which contain unique
            N-terminal domains, and multiple inhibitory domains that share
            homology with each other. Some isoforms may be tissue-specific. Two
            different pseudogenes of this gene are found on chromosome 19.
            [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (2) represents use of an alternate
            promoter and therefore differs in the 5' UTR and 5' coding region,
            compared to variant 1. These differences cause translation
            initiation at an alternate start codon and result in an isoform (2)
            with a shorter and distinct N-terminus. This variant also lacks one
            internal exons relative to variant 1. The resulting protein
            (isoform 2) has a distinct N-terminus and is shorter than isoform
            1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028843.1, AK028841.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                CD550676.1         1-39
            40-2931             AK028843.1         2-2893
            2932-3620           AK083395.1         2738-3426
            3621-3621           AK037098.1         995-995
            3622-3925           AK083395.1         3428-3731
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 40.52 cM"
     gene            1..3925
                     /gene="Cast"
                     /note="calpastatin"
                     /db_xref="GeneID:12380"
                     /db_xref="MGI:MGI:1098236"
     exon            1..142
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..2377
                     /gene="Cast"
                     /note="isoform 2 is encoded by transcript variant 2;
                     calpastatin type III; calpain inhibitor"
                     /codon_start=1
                     /product="calpastatin isoform 2"
                     /protein_id="NP_033947.1"
                     /db_xref="CCDS:CCDS26648.1"
                     /db_xref="GeneID:12380"
                     /db_xref="MGI:MGI:1098236"
                     /translation="MAFASWWYKTHVNEKNIGSSSKPGEKKGSDEKKAASLGSSQPSR
                     PHVGEAATATKVTASSAATSKSPSMSTTETKAVKTESKKPQSSEQPVVHEKKSKGGPK
                     EGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSEKPSESKTKFQDVPSAGGESVAG
                     GGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLIDTLGGHEDTN
                     RDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTD
                     QAIDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVIND
                     QALQALSDSLGTRQPDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKD
                     KDGKPLLPEPEETSKSLSESELIGELSADFDRSTYQDKPSTPAEKKSNDTSQTPPGET
                     VPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTREADPEHEKTVEDKVKEKAKE
                     EEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDALSQDFSSP
                     ANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQ
                     RPPDPDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPPTKKT
                     EKPDQDRDPIDALSEDLDSCPSTTETSKNTAKGKSKKTSSSKASKDGEKTKDSSKKTE
                     EVSKPKAKEDARHS"
     exon            143..205
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            206..277
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            278..337
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            338..376
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            377..430
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            431..544
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            545..634
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            635..703
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            704..802
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            803..883
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            884..922
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1009
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1099
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1201
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1285
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1333
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1423
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1424..1519
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1621
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1622..1705
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1756
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1757..1828
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1829..1927
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2032
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..2125
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2161
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2254
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2323
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            2324..2385
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     exon            2386..3925
                     /gene="Cast"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2539..2544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cast"
                     /note="hexamer: AATGAA"
     polyA_site      2557
                     /gene="Cast"
                     /note="major polyA site"
     regulatory      2774..2779
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cast"
                     /note="hexamer: AATAAT"
     polyA_site      2778
                     /gene="Cast"
     regulatory      2915..2920
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cast"
                     /note="hexamer: AATAAA"
     polyA_site      2967
                     /gene="Cast"
     polyA_site      2978
                     /gene="Cast"
     regulatory      3093..3098
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cast"
                     /note="hexamer: AATAAA"
     polyA_site      3116
                     /gene="Cast"
     regulatory      3152..3157
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cast"
                     /note="hexamer: ATTAAA"
     polyA_site      3184
                     /gene="Cast"
     regulatory      3624..3629
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cast"
                     /note="hexamer: AATGAA"
     polyA_site      3645
                     /gene="Cast"
ORIGIN      
        1 ggcagctgtg tcctgtgcgg tgtcagccgg gtcccagcca agcagagtgt catcgtggtg
       61 cacgttctgc gttctcgaga ggttcccagc ggagcaagtc agggttgtcg cgatggcatt
      121 cgcaagttgg tggtacaaga cgcatgttaa tgaaaagaat attggatcat cttctaaacc
      181 aggagaaaag aaaggatccg atgagaaaaa agcagcaagt ttggggagca gtcagccttc
      241 cagaccacat gttggtgaag ctgccacagc caccaaggtg acagcttcct ctgctgccac
      301 cagcaagtcc cccagcatga gtaccacaga gactaaggct gtgaaaacag agtctaagaa
      361 accacaatca agtgagcaac ctgtggttca cgagaaaaag tcaaaaggag ggccaaagga
      421 agggtcagag cccaaaaatc ttccgaagca cacatcaagt acaggaagca agcatgctca
      481 taaagaaaaa gcactttcca gatcaaatga acagatggtg tcagagaaac catcagaatc
      541 aaagacaaaa tttcaagatg ttccttcagc tggtggagag agtgtggctg gaggtgggac
      601 tgtggccact gccttggaca aagtggttgg taagaaaaaa gaacagaaac cattcacacc
      661 agcttcacct gtgcagtcca caccgagcaa gccatctgac aagtcaggca tggatgctgc
      721 cttggatgac ctgatagaca ccttaggtgg acatgaagat actaacagag atgatccacc
      781 atatactgga ccagtagttt tggacccaat gtattctacc taccttgagg cactgggtat
      841 aaaagaaggg actattcctc cagagtatag gaaacttttg gaaaaaaatg aagggatcac
      901 acaacctctt ccagactctc ctaaacctat ggggactgac caggctatag atgccttgtc
      961 atctgatttc acctgtagtt ctccaactgg aaagcaaagt gagaaagaga agtctactgg
     1021 ggaaattttc aaagctcagt ctgcaggagt gaccagaagt tctgttcccc ccaaggagaa
     1081 gaaaaggaag gtggaagagg aggtcataaa tgatcaagca cttcaggctc tgtcagattc
     1141 actgggcacc cggcagccag atcctccgag ccacgttagc caagctgaac aagtcaaaga
     1201 ggcaaaagca aaagaagaaa ggcaggagaa gtgtggtgaa gatgaggaca cagtcccagc
     1261 tgagtacagg ttaaaaccag caaaggataa agatggaaaa ccactattgc cagagcctga
     1321 agaaacatct aagagcctga gtgagtcgga gctgattggg gagctttcag cagattttga
     1381 ccgatctaca tatcaagaca aaccatctac gccagctgaa aagaaatcca atgacacatc
     1441 ccaaactcct ccgggggaga ctgtgcctcg ggcctccatg tgcagtatac ggtcagcgcc
     1501 acccaaacta gcatccttga agggcgtggt accagaagat gctgttgaaa ccttggctgg
     1561 aagcctgggg acaagggaag cagatccaga acatgaaaaa actgtggagg ataaagtcaa
     1621 ggagaaagct aaagaagaag agcatgaaaa acttggtgaa aaagaagaaa cagtgcctcc
     1681 tgattatcga ctagaagaag tcaaggataa ggatggaaaa ccactcctgc ccaaagaatc
     1741 ccaggaacaa cttgcaccct taagcgatga cttccttctt gatgccttgt ctcaggactt
     1801 ctccagtccg gcaaacatat cgtctcttga atttgaagat gccaaacttt ctgctgccat
     1861 ttctgaagta gtttctcaga cacctgctcc aagcacccat gcagcagctc cactgcctgg
     1921 cactgagcag aaagacaaag aacttgatga tgccttggat gaactttctg acagtcttgg
     1981 acaaaggccg cctgatccag atgagaacaa accactggat gacaaagtga aggagaaaat
     2041 caaaccagag catagcgaga aactgggaga aagagacgac accatccccc ctgaatacag
     2101 gcatctcttg gataatgatg ggaaggacaa accagagaag ccaccgacta agaaaacaga
     2161 gaaacctgat caggaccggg accccattga tgccctctca gaagatttgg atagctgccc
     2221 ctcaactaca gagacctcaa agaatacagc aaaggggaag agcaagaaga cttcaagttc
     2281 caaagcatcc aaggacggag agaaaacaaa ggactcttcc aagaagacag aggaagtgtc
     2341 caagccaaag gctaaagaag atgcaagaca cagttaatga atttgggtat ctgaatagaa
     2401 attcttcagc tggtggatgg tgacttttga agaacaaagg ctttgatgac agttttttct
     2461 gggtggtttc tggacagtgg tatttgttat gtcttttgac atcttaaaca ctgagttgtt
     2521 attcttttcc agaacgaaaa tgaatttgtc tgcttcacct gtgttctgta ttgagtattg
     2581 ataaacttca aagattttta attgccctta gatgggagaa ggaagcttta tatttgtaag
     2641 aaatatattt gatagtttct taaaacacca aaaatcccag aaagccttta caaacctttg
     2701 cactttatat atacagtgcc tgtaaatctc attagtattt ttggtttgca ctaaattttt
     2761 ttagcatttg aaaaataatg ctcccaactt ttgtcagtat tttgatttct tattatgtct
     2821 ttttcttcag ctttgatgta tctttgggtt aatgttgata agtcaaacat gaaatattgt
     2881 atactcggtg tataactctt ttggggatgc taataataaa ttaatgatag atgacccaag
     2941 gtaaaccttg ggagcatcaa tctcctttcc tgcctgaact ctactttctt aggaatgagg
     3001 acttctactc atggtcaaac aagacacact gagacttgaa tgaagctagc agaaaaacag
     3061 cacagactag agtttaacgt ataactttta gcaataaatt ggggattctt agaatactca
     3121 tatggcttga actatcctgg tttaccatgg cattaaactg tggtttccac cctctacatg
     3181 aaaaccagat caaaatcctt tgcttatagt cacttgccac attgtctaaa gcatctttac
     3241 caaacacaaa aatcaacttc tttaaatgtg tgtatgtaca tacaatggat acactgaaat
     3301 ttaaaatctg ctgctatatc aaagcaggtt aaattatctt aacatctttg aatggtacct
     3361 aagatttttc tgagcactgt actggtatca ttgatggaaa tttgataaac aattaattcc
     3421 atttctactt cctagcaatg aatgctgaac aagatgtcag gtgcatataa accaaacttc
     3481 aactttcttg tgtccagaaa tcacagcctt tgttgccatc aatgtaaaga ttttactacg
     3541 atttaattaa ctttactaaa tataacttac atcatgagca attcccaggg gataggtaag
     3601 accaagcaaa acattatatc cataatgaac atgatacttt gttaactgtc tctaaaatta
     3661 ctctctacaa aatatgcatg cacctcttag atttcaaatg ctacccacaa aagtatttaa
     3721 ttcccagtcc tataaaaggg acagtcacta ttctgttttt aaggcactga taagaatgta
     3781 ctccctgggc ggtggtggcg cacgccttta atcccagcac ttgggaggca gaggcaagca
     3841 gatttctgag ttcgaggcca gcctggtcta caaagtgagt tccaggacag ccagggctac
     3901 acagagaaac cctgtctcga aaagc
//
