LOCUS       NM_001379465            3679 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Cas scaffolding protein family member 4 (Cass4),
            transcript variant 5, mRNA.
ACCESSION   NM_001379465 XM_030251764
VERSION     NM_001379465.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Lu,W., Quintero-Rivera,F., Fan,Y., Alkuraya,F.S., Donovan,D.J.,
            Xi,Q., Turbe-Doan,A., Li,Q.G., Campbell,C.G., Shanske,A.L.,
            Sherr,E.H., Ahmad,A., Peters,R., Rilliet,B., Parvex,P.,
            Bassuk,A.G., Harris,D.J., Ferguson,H., Kelly,C., Walsh,C.A.,
            Gronostajski,R.M., Devriendt,K., Higgins,A., Ligon,A.H.,
            Quade,B.J., Morton,C.C., Gusella,J.F. and Maas,R.L.
  TITLE     NFIA haploinsufficiency is associated with a CNS malformation
            syndrome and urinary tract defects
  JOURNAL   PLoS Genet 3 (5), e80 (2007)
   PUBMED   17530927
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL833787.8.
            
            On Oct 27, 2022 this sequence version replaced NM_001379465.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR6116558.64167.1,
                                           SRR13861859.3670068.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-185               AL833787.8         36849-37033
            186-608             AL833787.8         59148-59570
            609-710             AL833787.8         66627-66728
            711-791             AL833787.8         67628-67708
            792-2081            AL833787.8         69664-70953
            2082-2159           AL833787.8         72670-72747
            2160-3679           AL833787.8         75261-76780
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 94.85 cM"
     gene            1..3679
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
                     /note="Cas scaffolding protein family member 4"
                     /db_xref="GeneID:320664"
                     /db_xref="MGI:MGI:2444482"
     exon            1..185
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             150..2570
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
                     /note="isoform 5 is encoded by transcript variant 5;
                     likely orthologue of H. sapiens chromosome 20 open reading
                     frame 32 (C20orf32)"
                     /codon_start=1
                     /product="cas scaffolding protein family member 4 isoform
                     5"
                     /protein_id="NP_001366394.1"
                     /db_xref="GeneID:320664"
                     /db_xref="MGI:MGI:2444482"
                     /translation="MRGTSIREGAPKTLLARALYDNHADCSDELAFSRGDILTIVEQN
                     VPESEGWWRCLLHGRQGLAPANRLQVLRETPADRPCPLLPRGPDTDLTSSGAPYQVQD
                     LISPPPQGPVYEPMRSWVEGPSPATAQVYELPESPSSARIICEKTLSFPKQALSVLPR
                     PTRASLPTLPSQVYDVPVQRQGFSTLERLEKQQFYDIPTSSQKALLHSSTSQASGRDV
                     TLAPTMAFRQGGGYNPLSSPQKSERIHDTPVLLEKADVRNVSMTSFTKDSGSRAIPGS
                     SAVHTGAVALSPQLGNTVQRKNSLPEEPTYAFPTSRDPLPSDAGGSYKVPSRFLIPRV
                     EQQNTMPNIYDTPKAMQGVSHNAPKAMQGVSLAGKELERGREAPENSPWISGQTSFLS
                     PDSDRLSVASSDSRASVVSSCSSISMDSSSGSSSEDSVKELWMDVDFAKETAVSLQHK
                     VASSAAGLLLFVSRTWRFKDSLETNIHRIRRAADHVEESVREFLDFAQGVGGTACNLT
                     DSYLQARIRDQLQTISSSYQTLLDAKGSLDRCNWSLEVLVTDKVQNSLDDLERFVATA
                     RIVPEDVKRFTSIVIANGKLLFKQNCEKGEMDLKCERCIRPPQRETESYQESSPFDRQ
                     PTTEHSFELARKNRVNVCWQQSPNLQEKGKPTMEGKSNRNPDFHGMSPPPLTSPSPSG
                     QNTERKIHLSKHSRLYFGALFKAISVFASSLSNGQPPEVFITQSKLVITVGQKLVDTL
                     CSETQEKDERNEILCGSSHLCGLLKDLALATKSAVIQYPSPSALSLLQSEVERLEHHS
                     RKFRDTLE"
     exon            186..608
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            609..710
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            711..791
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            792..2081
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2082..2159
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..3679
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3546..3551
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3582
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
                     /note="major polyA site"
     regulatory      3652..3657
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      3679
                     /gene="Cass4"
                     /gene_synonym="F730031O20Rik"
ORIGIN      
        1 atctcgactg tttccatgtg ttggcaaaca gttccataga atccagctcc tgggaaccag
       61 ccagcagcat gcagtgatat tgcatcattg gtccctggaa ccagatatat cagcggcaga
      121 actccgagga ccctgctcac atcacagcca tgagaggcac cagcatcaga gagggtgcgc
      181 ccaagaccct gttggccagg gcactctacg acaaccatgc tgattgctcc gacgagcttg
      241 ctttctccag aggggacatc ctgaccatcg tggaacaaaa tgtgccagaa agcgagggct
      301 ggtggaggtg tctgctccac gggaggcaag gcctggctcc agccaaccgc ctccaagtcc
      361 tcagagagac ccctgcagac agaccctgtc ccctgctccc gagaggccca gacacagatc
      421 tcaccagctc cggggcgcct taccaggtgc aggacttgat ctccccgcca ccccagggac
      481 ctgtgtatga accaatgagg agctgggtgg aggggccgtc accagcgact gcccaagtct
      541 acgaattgcc tgagtcacct tccagtgcca ggatcatctg tgaaaagacg ctgagcttcc
      601 ccaagcaggc cctctctgtg cttcccagac cgacgagggc ctcgttgccg acacttcctt
      661 cccaggtgta tgacgtgcct gtccagaggc agggcttctc aacccttgag aggctggaga
      721 agcagcagtt ctatgacata cccaccagct cccagaaggc tctgctccac tcctccacca
      781 gccaggcaag tggacgggat gttacattgg caccgacaat ggccttcaga caaggtggtg
      841 gctataaccc gttatccagc cctcagaagt cagaacggat tcatgacact ccggtgttgc
      901 tggaaaaggc cgatgtcaga aatgtgtcta tgaccagctt caccaaagac tcaggttcca
      961 gagccattcc aggctcctct gctgtccaca ctggagctgt ggccctctcc ccacagctgg
     1021 ggaacactgt gcagagaaaa aacagcctcc cagaagaacc tacctatgcc ttcccaacgt
     1081 ccagggaccc actgccctct gatgcaggtg gcagctacaa ggtcccttcc aggtttctta
     1141 ttcccagagt ggagcaacag aacaccatgc ctaacattta tgacacccct aaagccatgc
     1201 agggtgtgtc tcataacgcc cctaaagcca tgcagggtgt gtctctggct ggaaaggagc
     1261 tggagagagg cagagaggct ccagaaaatt ccccctggat ctccggacag acaagtttcc
     1321 tgtctcctga ctcggaccga ttatctgttg ccagctcaga cagcagggct agtgtggtgt
     1381 cttcctgctc ctccatatcc atggactcct cctctggctc ctcctcagag gactccgtca
     1441 aggagctatg gatggacgtg gactttgcaa aggagacagc agtgtctctg caacacaaag
     1501 tggccagctc ggcggcgggc ctgctgctct ttgtaagcag gacttggagg ttcaaagact
     1561 ccttggaaac caacatccac agaatccgca gggccgctga ccacgtagag gaatctgtaa
     1621 gagaatttct ggattttgcc caaggggttg gtgggaccgc ttgcaatctc acagacagct
     1681 accttcaggc tagaattaga gatcagctcc agacaatctc tagctcctac cagacactgc
     1741 tggatgccaa gggaagcttg gaccgctgca attggtccct ggaagttctt gtgacagaca
     1801 aagtccaaaa cagcctggat gacctggaga ggtttgtcgc cactgcacga atagttccag
     1861 aagacgtcaa gaggtttacc tccatcgtca ttgccaatgg gaagctcctt tttaaacaga
     1921 actgtgagaa aggagagatg gacttgaagt gtgagagatg catccggcct ccccaaagag
     1981 aaactgagtc ctaccaagag agcagcccct tcgacaggca accgacaact gaacactcct
     2041 ttgaactagc aaggaaaaac agagtgaatg tctgttggca gcagtcacct aacctacaag
     2101 agaaaggaaa gcccaccatg gaaggaaagt caaacagaaa cccagacttc catggcatga
     2161 gtcctccccc tcttacctct ccatctccca gtgggcaaaa caccgagagg aagatccacc
     2221 tgtctaagca tagcagactc tatttcggag cactcttcaa agccatcagt gtctttgcaa
     2281 gcagcctcag caacggccag ccccccgagg tcttcatcac tcagagcaag ctagtcatca
     2341 cagttggaca gaagctggtg gacacgctgt gcagtgagac ccaggagaaa gatgaacgca
     2401 atgagatcct gtgtggcagc agtcacctct gcgggctgct caaggacctg gcgctggcca
     2461 ccaaaagtgc agtgatccag tacccgagcc ccagtgccct gagcctcctc cagtcagagg
     2521 tggagaggct agagcaccac tctcggaaat tcagagacac gttggaatga agcctgcgga
     2581 ggtccgctcc cggccagttt cacacccacc attctgttcc gtggaaaagc caatcagaat
     2641 ggctgaaaca gccacgggtg tcagagctgg tgttagtaag tggctaagcc tccgaaggcc
     2701 actgactgac cacgaggtca agaaagagag gcaggcgaca agtcaagacc ttgagatgct
     2761 gaactcactg agccgaggtg ttcagcatca acaggggcag tggcattgta ggagccacgt
     2821 ttcgtgtcag tcaaatgagt gtgtggctta cagtcactct acatttgtta ggtagcgcct
     2881 aaggtcaagg tcaaggagtt gaagagacag cagtgtgaaa caggctctga cctcacagag
     2941 gcaccatcta attgggaaga cagacaaagg gacaggctag actagtccac agtaagctcc
     3001 aagccaaaga ctatatagta agaaactatt tttttaaagc ctcctgaaga gtaatgagtt
     3061 tgcctttttt caatcagttg ttttagatga atactgtgag atgtatacaa actgtttatg
     3121 cacaaacacc tgtgtgcatg cacgcgtgtg catgtgttgc tagaaactta atctagggtt
     3181 tcacaaatgc taagcatgta ctctgccacg aagactgatc cctagtcccc caaatgtatg
     3241 ctgttacttc accccaaagg tgacaattat gtgctctgtt gagtccccta ctaacttatt
     3301 ttgtgttaca aatcctgtat tgatctgttc ccctgctcag ttagattagg tttatatttc
     3361 actagtctca aatctgttat attatttatc tataatcttg ggtttaaaca tacttgcaag
     3421 gtcctgatgg ctcaggaggt agaggcacct aaccccagtc ctgatttcct gagttctatc
     3481 ccagggaccc acgtggtgga aggagaggag actcctgata actgtcttca gacatccatg
     3541 cacaaaataa aacgaaactt gtaattaaaa acttaaaaca tacgagcaag ctggcagcaa
     3601 tcctttctct gaaagggcct ctgagccgtg tcacctgagt gctggtacca aagtaaagtg
     3661 tttgttaaag acacctcca
//
