LOCUS       NM_001284409            2610 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus caspase 3 (Casp3), transcript variant 1, mRNA.
ACCESSION   NM_001284409
VERSION     NM_001284409.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Serdar,L.D., Egol,J.R., Lackford,B., Bennett,B.D., Hu,G. and
            Silver,D.L.
  TITLE     mRNA stability fine-tunes gene expression in the developing cortex
            to control neurogenesis
  JOURNAL   PLoS Biol 23 (2), e3003031 (2025)
   PUBMED   39913536
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Stottmann,R.W., Moran,J.L., Turbe-Doan,A., Driver,E., Kelley,M. and
            Beier,D.R.
  TITLE     Focusing forward genetics: a tripartite ENU screen for
            neurodevelopmental mutations in the mouse
  JOURNAL   Genetics 188 (3), 615-624 (2011)
   PUBMED   21515572
  REMARK    GeneRIF: Mutation in the planar cell polarity gene, Scrib, and a
            mutation in caspase-3 gene have the most profound effect in
            controlling neural development.
REFERENCE   3  (bases 1 to 2610)
  AUTHORS   Parker,A., Hardisty-Hughes,R.E., Wisby,L., Joyce,S. and Brown,S.D.
  TITLE     Melody, an ENU mutation in Caspase 3, alters the catalytic cysteine
            residue and causes sensorineural hearing loss in mice
  JOURNAL   Mamm Genome 21 (11-12), 565-576 (2010)
   PUBMED   21116635
  REMARK    GeneRIF: A point mutation in Casp3 alters the catalytic cysteine
            residue and causes sensorineural hearing loss.
REFERENCE   4  (bases 1 to 2610)
  AUTHORS   Li,Z., Jo,J., Jia,J.M., Lo,S.C., Whitcomb,D.J., Jiao,S., Cho,K. and
            Sheng,M.
  TITLE     Caspase-3 activation via mitochondria is required for long-term
            depression and AMPA receptor internalization
  JOURNAL   Cell 141 (5), 859-871 (2010)
   PUBMED   20510932
  REMARK    GeneRIF: Hippocampal slices from caspase-3 knockout mice lose
            specifically their ability to undergo NMDA receptor-dependent
            long-term depression; these findings reveal a critical role of the
            mitochondrial pathway of caspase activation in synaptic depression.
REFERENCE   5  (bases 1 to 2610)
  AUTHORS   Miura,M., Chen,X.D., Allen,M.R., Bi,Y., Gronthos,S., Seo,B.M.,
            Lakhani,S., Flavell,R.A., Feng,X.H., Robey,P.G., Young,M. and
            Shi,S.
  TITLE     A crucial role of caspase-3 in osteogenic differentiation of bone
            marrow stromal stem cells
  JOURNAL   J Clin Invest 114 (12), 1704-1713 (2004)
   PUBMED   15599395
  REMARK    GeneRIF: caspase-3 is crucial for the differentiation of bone
            marrow stromal stem cells by influencing TGF-beta/Smad2 pathway and
            cell cycle progression
REFERENCE   6  (bases 1 to 2610)
  AUTHORS   Morishita,H., Makishima,T., Kaneko,C., Lee,Y.S., Segil,N.,
            Takahashi,K., Kuraoka,A., Nakagawa,T., Nabekura,J., Nakayama,K. and
            Nakayama,K.I.
  TITLE     Deafness due to degeneration of cochlear neurons in
            caspase-3-deficient mice
  JOURNAL   Biochem Biophys Res Commun 284 (1), 142-149 (2001)
   PUBMED   11374883
  REMARK    GeneRIF: cochlear neuron function
REFERENCE   7  (bases 1 to 2610)
  AUTHORS   Keramaris,E., Stefanis,L., MacLaurin,J., Harada,N., Takaku,K.,
            Ishikawa,T., Taketo,M.M., Robertson,G.S., Nicholson,D.W.,
            Slack,R.S. and Park,D.S.
  TITLE     Involvement of caspase 3 in apoptotic death of cortical neurons
            evoked by DNA damage
  JOURNAL   Mol Cell Neurosci 15 (4), 368-379 (2000)
   PUBMED   10845773
REFERENCE   8  (bases 1 to 2610)
  AUTHORS   Woo,M., Hakem,R., Soengas,M.S., Duncan,G.S., Shahinian,A., Kagi,D.,
            Hakem,A., McCurrach,M., Khoo,W., Kaufman,S.A., Senaldi,G.,
            Howard,T., Lowe,S.W. and Mak,T.W.
  TITLE     Essential contribution of caspase 3/CPP32 to apoptosis and its
            associated nuclear changes
  JOURNAL   Genes Dev 12 (6), 806-819 (1998)
   PUBMED   9512515
REFERENCE   9  (bases 1 to 2610)
  AUTHORS   Kuida,K., Zheng,T.S., Na,S., Kuan,C., Yang,D., Karasuyama,H.,
            Rakic,P. and Flavell,R.A.
  TITLE     Decreased apoptosis in the brain and premature lethality in
            CPP32-deficient mice
  JOURNAL   Nature 384 (6607), 368-372 (1996)
   PUBMED   8934524
REFERENCE   10 (bases 1 to 2610)
  AUTHORS   Juan,T.S., McNiece,I.K., Jenkins,N.A., Gilbert,D.J., Copeland,N.G.
            and Fletcher,F.A.
  TITLE     Molecular characterization of mouse and rat CPP32 beta gene
            encoding a cysteine protease resembling interleukin-1 beta
            converting enzyme and CED-3
  JOURNAL   Oncogene 13 (4), 749-755 (1996)
   PUBMED   8761296
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC119267.8.
            
            Summary: This gene encodes a protein that belongs to a highly
            conserved family of cysteinyl aspartate-specific proteases that
            function as essential regulators of programmed cell death through
            apoptosis. Members of this family contain an N-terminal pro-domain
            and require cleavage at specific aspartate residues to become
            mature. The protein encoded by this gene belongs to a subgroup of
            cysteinyl aspartate-specific proteases that are activated by
            initiator caspases and that perform the proteolytic cleavage of
            apoptotic target proteins. Mice defective for this gene exhibit a
            variety of phenotypes including reduced neuronal apoptosis
            resulting in hyperplasias, hearing loss, attenuated osteogenic
            differentiation of bone marrow stromal stem cells, and pre- and
            post-natal lethality. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146226.1, BC038825.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               AC119267.8         104628-104838       c
            212-279             AC119267.8         92328-92395         c
            280-404             AC119267.8         89688-89812         c
            405-533             AC119267.8         87803-87931         c
            534-709             AC119267.8         86577-86752         c
            710-830             AC119267.8         85807-85927         c
            831-2610            AC119267.8         82431-84210         c
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 26.39 cM"
     gene            1..2610
                     /gene="Casp3"
                     /gene_synonym="A830040C14Rik; AC-3; CASP-3; Caspase-3;
                     CC3; CPP-32; CPP32; Lice; mldy; SCA-1; Yama"
                     /note="caspase 3"
                     /db_xref="GeneID:12367"
                     /db_xref="MGI:MGI:107739"
     exon            1..211
                     /gene="Casp3"
                     /gene_synonym="A830040C14Rik; AC-3; CASP-3; Caspase-3;
                     CC3; CPP-32; CPP32; Lice; mldy; SCA-1; Yama"
                     /inference="alignment:Splign:2.1.0"
     exon            212..279
                     /gene="Casp3"
                     /gene_synonym="A830040C14Rik; AC-3; CASP-3; Caspase-3;
                     CC3; CPP-32; CPP32; Lice; mldy; SCA-1; Yama"
                     /inference="alignment:Splign:2.1.0"
     CDS             227..1060
                     /gene="Casp3"
                     /gene_synonym="A830040C14Rik; AC-3; CASP-3; Caspase-3;
                     CC3; CPP-32; CPP32; Lice; mldy; SCA-1; Yama"
                     /EC_number="3.4.22.56"
                     /note="protein Yama; cysteine protease CPP32; SREBP
                     cleavage activity 1; caspase 3, apoptosis related cysteine
                     protease; apopain"
                     /codon_start=1
                     /product="caspase-3"
                     /protein_id="NP_001271338.1"
                     /db_xref="CCDS:CCDS22294.1"
                     /db_xref="GeneID:12367"
                     /db_xref="MGI:MGI:107739"
                     /translation="MENNKTSVDSKSINNFEVKTIHGSKSVDSGIYLDSSYKMDYPEM
                     GICIIINNKNFHKSTGMSSRSGTDVDAANLRETFMGLKYQVRNKNDLTREDILELMDS
                     VSKEDHSKRSSFVCVILSHGDEGVIYGTNGPVELKKLTSFFRGDYCRSLTGKPKLFII
                     QACRGTELDCGIETDSGTDEEMACQKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQS
                     LCSMLKLYAHKLEFMHILTRVNRKVATEFESFSLDSTFHAKKQIPCIVSMLTKELYFY
                     H"
     exon            280..404
                     /gene="Casp3"
                     /gene_synonym="A830040C14Rik; AC-3; CASP-3; Caspase-3;
                     CC3; CPP-32; CPP32; Lice; mldy; SCA-1; Yama"
                     /inference="alignment:Splign:2.1.0"
     exon            405..533
                     /gene="Casp3"
                     /gene_synonym="A830040C14Rik; AC-3; CASP-3; Caspase-3;
                     CC3; CPP-32; CPP32; Lice; mldy; SCA-1; Yama"
                     /inference="alignment:Splign:2.1.0"
     exon            534..709
                     /gene="Casp3"
                     /gene_synonym="A830040C14Rik; AC-3; CASP-3; Caspase-3;
                     CC3; CPP-32; CPP32; Lice; mldy; SCA-1; Yama"
                     /inference="alignment:Splign:2.1.0"
     exon            710..830
                     /gene="Casp3"
                     /gene_synonym="A830040C14Rik; AC-3; CASP-3; Caspase-3;
                     CC3; CPP-32; CPP32; Lice; mldy; SCA-1; Yama"
                     /inference="alignment:Splign:2.1.0"
     exon            831..2610
                     /gene="Casp3"
                     /gene_synonym="A830040C14Rik; AC-3; CASP-3; Caspase-3;
                     CC3; CPP-32; CPP32; Lice; mldy; SCA-1; Yama"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2580..2585
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Casp3"
                     /gene_synonym="A830040C14Rik; AC-3; CASP-3; Caspase-3;
                     CC3; CPP-32; CPP32; Lice; mldy; SCA-1; Yama"
                     /note="hexamer: CATAAA"
     polyA_site      2601
                     /gene="Casp3"
                     /gene_synonym="A830040C14Rik; AC-3; CASP-3; Caspase-3;
                     CC3; CPP-32; CPP32; Lice; mldy; SCA-1; Yama"
                     /note="major polyA site"
ORIGIN      
        1 ggggctgaaa ccaccaatcg cgactggcgt gtgcgagatg aggtgttggg gggtgtgtga
       61 ggggggatgg ggcggggagg tagagaaggg gcgtggcgtg gggcgtgacc ggcggtagtc
      121 tgctgttgct gctgctgctg ctgcagttgc agtgttggtg ctggtgggat caaagcgcag
      181 tgtcctgcgg cggggagctt ggaacggtac gctaagaaaa gtgaccatgg agaacaacaa
      241 aacctcagtg gattcaaaat ccattaataa ttttgaagta aagaccatac atgggagcaa
      301 gtcagtggac tctgggatct atctggacag tagttacaaa atggattatc ctgaaatggg
      361 catatgcata ataattaata ataagaactt ccataagagc actggaatgt catctcgctc
      421 tggtacggat gtggacgcag ccaacctcag agagacattc atgggcctga aataccaagt
      481 caggaataaa aatgatctta ctcgtgaaga cattttggaa ttaatggata gtgtttctaa
      541 ggaagatcat agcaaaagga gcagctttgt gtgtgtgatt ctaagccatg gtgatgaagg
      601 ggtcatttat gggacaaatg ggcctgttga actgaaaaag ttgactagct tcttcagagg
      661 cgactactgc cggagtctga ctggaaagcc gaaactcttc atcattcagg cctgccgggg
      721 tacggagctg gactgtggca ttgagacaga cagtgggact gatgaggaga tggcttgcca
      781 gaagataccg gtggaggctg acttcctgta tgcttactct acagcacctg gttactattc
      841 ctggagaaat tcaaaggacg ggtcgtggtt catccagtcc ctttgcagca tgctgaagct
      901 gtacgcgcac aagctagaat ttatgcacat tctcactcgc gttaacagga aggtggcaac
      961 ggaattcgag tccttctccc tggactccac tttccacgca aagaaacaga tcccgtgtat
     1021 tgtgtccatg ctcacgaaag aactgtactt ttatcactag aggaatgatt gggggtgggg
     1081 gggggcgtgt ttctgttttg ttatgccaaa tgagaaagct gtcagggaga ctctcattta
     1141 aatctaatct gacggtcctc ctggtctttg tacgctacca ctgcctagca atgcagccag
     1201 ccacagtgca gctacctcaa cttcgacatc aggtagttga aatgaaattt aatttaataa
     1261 ggagcaagta actgtcaatg atggtactat catcctagat gaaattacaa agttgccctt
     1321 ttataattag caagatttgg cgatactatg aattttgaag tcattttgaa gcagtacagc
     1381 ttttccactg atgattttat actccccact catgttaaga atgttgttct agttttcgtt
     1441 aaacgtagaa acaataatgt caaatgataa tgtcttagaa cttgaatcca tgagcagagt
     1501 caaaggctgg aacccttgtt ttggacctga tttatggaag tgaagagttg gaccaccata
     1561 gcatgcatta tagctactgg ttttgtgaca gttgtccaca acacgtattt gttattttaa
     1621 aaaaaaaaat catgttttat tactggaaca aaaataactt gagtatttat gtgagaggaa
     1681 aaccgtgtgc gaacttggtt gactcattga aggcaaggtg ctaaaatcca tcagcatttg
     1741 tttcttactt atccttatac aaatcagctc ggtcttacag accagcaaaa tatctcaaga
     1801 gctgttatgt aacagcctca aatggttgat tattagaatg aagtacagga attctatagg
     1861 aatatactat aataccagtt gtcaaacaga gcttaagaaa aggagagatg aaatctactg
     1921 tagttggaga gaatgtggtt tgagccagag cagagacttg gcagagccat gggcacatct
     1981 tcagaaaagg cctgccagct ggactcccca cccctcaccc cacccccctt gtgagttctg
     2041 gtttgtgtgg atgatgtcca agttaccacg tgggaaagtg aaccagatca caaacttctg
     2101 caaaaaggct ttcccagagg gctgagagtc tgaagaaagt tcactggact ccatgtccag
     2161 agtctgtagc atgctgactt ttctgttgac gtttacaaag aaaggaaaaa taatggtgat
     2221 atttatactg tgctagaaac gaaagggcca gggcacacag gacttggaaa gcatccagca
     2281 atagccctgc agcccatgtg gagaagctgc gggctgcagt ccatcctcag ggagactgtg
     2341 ctctgctcct ggcctcctta tgggggtgct gagtcactgc tggctctcgc agcatttctc
     2401 ctaagcttcc ttgttgcaca aagaaaggaa ggaactcaca ttgtaacgga tagattcttg
     2461 tttagactgg ctttgttgtt aacgctttct aagtgaatgc agcatgtgat ggtattttaa
     2521 agtgtgttcc catgtgacaa ttttataaaa gtttgttacc ttgcaatttt atttcaaaac
     2581 ataaattcag atttaaaatt atcagtgtat
//
