LOCUS       NM_001359137            1864 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus cysteinyl-tRNA synthetase 2, mitochondrial (Cars2),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001359137 XM_006508893
VERSION     NM_001359137.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Akaike,T., Ida,T., Wei,F.Y., Nishida,M., Kumagai,Y., Alam,M.M.,
            Ihara,H., Sawa,T., Matsunaga,T., Kasamatsu,S., Nishimura,A.,
            Morita,M., Tomizawa,K., Nishimura,A., Watanabe,S., Inaba,K.,
            Shima,H., Tanuma,N., Jung,M., Fujii,S., Watanabe,Y., Ohmuraya,M.,
            Nagy,P., Feelisch,M., Fukuto,J.M. and Motohashi,H.
  TITLE     Cysteinyl-tRNA synthetase governs cysteine polysulfidation and
            mitochondrial bioenergetics
  JOURNAL   Nat Commun 8 (1), 1177 (2017)
   PUBMED   29079736
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1864)
  AUTHORS   Vats,K.R., Ishwad,C., Singla,I., Vats,A., Ferrell,R., Ellis,D.,
            Moritz,M., Surti,U., Jayakar,P., Frederick,D.R. and Vats,A.N.
  TITLE     A locus for renal malformations including vesico-ureteric reflux on
            chromosome 13q33-34
  JOURNAL   J Am Soc Nephrol 17 (4), 1158-1167 (2006)
   PUBMED   16565260
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK038968.1 and BF458069.1.
            
            On Dec 21, 2017 this sequence version replaced XM_006508893.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK038968.1, SRR7652917.959645.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AK038968.1         13-29
            18-1843             AK038968.1         31-1856
            1844-1864           BF458069.1         1-21                c
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 5.73 cM"
     gene            1..1864
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /note="cysteinyl-tRNA synthetase 2, mitochondrial"
                     /db_xref="GeneID:71941"
                     /db_xref="MGI:MGI:1919191"
     exon            1..273
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..1750
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /EC_number="6.1.1.16"
                     /note="isoform 1 is encoded by transcript variant 1;
                     probable cysteine--tRNA ligase, mitochondrial; probable
                     cysteinyl-tRNA synthetase, mitochondrial; cysteinyl-tRNA
                     synthetase 2 (mitochondrial)(putative)"
                     /codon_start=1
                     /product="probable cysteine--tRNA ligase, mitochondrial
                     isoform 1"
                     /protein_id="NP_001346066.1"
                     /db_xref="GeneID:71941"
                     /db_xref="MGI:MGI:1919191"
                     /translation="MLRARGPGPGALLLRAALGLGRRGRSWQRPQGQDTGVQVHNSLT
                     GRKEPLIVARSDAVSWYSCGPTVYDHAHLGHACSYVRFDIIRRILTRVFGCNVVMAMS
                     ITDVDDKIIKRANEMNVTPASLASLFEEEFKQDMAALKVLPPTVYLRVTENIPQIIAF
                     IEGIIAHGHAYSTATGSVYFDLHARGDKYGKLVNTVPSATAEPGDSDKRHSSDFALWK
                     AAKPQEVFWASPWGDGRPGWHIECSTMASEVFGSHLDIHTGGIDLAFPHHENEIAQSE
                     VFHQCQQWGNYFLHSGHLHVKGTEEKMSKSLKNYITIKDFLKTFSPDVFRLFCLRTNY
                     RSAIEYSDSTLVEAKHLLLGLASFVEDARAYVKGQLTCGPVEEDVLWERLTSTKKAVK
                     AALANDFDTPRAVNTILDLVHHANRQLRAVSKEASGPRSPTVFGAIISYVEQFFETVG
                     ISLANRQCVSGDSSTVTLRCVVDELVRFRLKVRQYALDTPGAAGEARKRQLQERQPLL
                     EACDTLRQDLVTHGINVKDRGSAASTWELLDPRTRHQKPGDRG"
     exon            274..324
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            325..442
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            443..514
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            515..620
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            621..704
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            705..834
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            835..968
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1036
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1103
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1242
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1366
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1465
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..1672
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1847
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1814..1819
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      1847
                     /gene="Cars2"
                     /gene_synonym="2310051N18Rik; 2410044A07Rik; cysRS;
                     D530030H10Rik"
                     /note="major polyA site"
ORIGIN      
        1 gccccatgag gaacgggcgt ggcttctggt tcgcccggct gggcctggcc tgcaggatct
       61 tgccttccgc ctgttcgctg gatctgtccc caatatgctg cgggcgcgcg ggcctgggcc
      121 cggcgctctg ctgctccggg ccgcgctggg tctcgggcgg cgcgggcggt catggcaacg
      181 gccgcaaggc caggacaccg gcgtgcaggt gcataacagc ctcactggca ggaaggagcc
      241 gctgatagtg gcccgctcgg acgcagtctc ctggtatagc tgtggaccga ctgtgtacga
      301 ccatgcacat cttggccatg cttgctccta tgttagattt gacattatcc ggaggatcct
      361 gaccagagtg tttggatgca atgtggtcat ggcgatgagc attaccgacg tggatgacaa
      421 aataatcaag agagctaacg agatgaacgt gacgcccgct tctctcgcca gtctttttga
      481 ggaagaattt aaacaagaca tggcagccct gaaggttctg ccgccaactg tgtatttgag
      541 agtcactgag aacatccctc agatcattgc tttcatcgag ggcatcattg ctcacggcca
      601 tgcatactcg acagctacag gcagcgtcta cttcgatctg cacgcccgag gggacaagta
      661 tggcaagctg gtcaacacgg ttcccagtgc cactgcagag ccaggtgact ctgacaagcg
      721 gcacagcagc gacttcgccc tgtggaaggc agccaaacct caggaggtgt tctgggcttc
      781 gccgtgggga gacggacggc ctggatggca catcgagtgc tctaccatgg ccagtgaggt
      841 gttcggaagc cacctggaca tccacaccgg cggcatagac ttggctttcc cacatcacga
      901 aaatgaaatc gcgcagagtg aagtcttcca ccagtgtcag cagtggggaa attacttcct
      961 acattctggt catttgcatg tgaaaggcac agaagaaaag atgtccaaat ccctaaaaaa
     1021 ctatatcacc attaaggact tcctgaagac cttctcccct gacgtcttcc ggcttttctg
     1081 cttgcgtacc aactataggt cagccattga atacagtgac agtaccttgg tggaagccaa
     1141 gcacctcctg ctggggctgg cctcttttgt ggaggatgca cgagcctacg taaaggggca
     1201 gctgacctgt ggccctgttg aggaggatgt gctatgggag aggctgacta gcaccaagaa
     1261 ggcggtgaaa gctgctctag ccaatgactt tgacacaccg agggcagtga acaccatcct
     1321 ggaccttgtg caccatgcaa acagacagct cagggcggtc tccaaggaag ctagtggccc
     1381 aaggagtccc actgtgtttg gggccatcat ttcctacgtt gagcagtttt tcgagactgt
     1441 tgggatttct ctggcaaatc gacagtgtgt ttcaggagac agcagcacag taacgttgcg
     1501 ctgtgtggta gatgaactcg tgcgcttcag gctgaaggtc cgccagtatg cactggacac
     1561 acccggggct gctggggagg ctcggaaacg gcagctccag gaaaggcagc ccttgctgga
     1621 ggcatgtgac acactccgac aggacctcgt cacccatggc atcaatgtta aggacagagg
     1681 cagtgcagcc tccacatggg aactgctgga tccaaggaca agacaccaga aacctgggga
     1741 ccgaggatga ggtggctgca tcccatgcgc tgccataccc tggccttctg ctcttttgtg
     1801 atgaaaactt tatattaaag ttgccaattg tggctgttta gccaataaaa aaaaaaaaaa
     1861 aaaa
//
