LOCUS       NM_013742               3236 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus cysteinyl-tRNA synthetase 1 (Cars1), transcript
            variant 1, mRNA.
ACCESSION   NM_013742
VERSION     NM_013742.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Di Michele,M., Attina,A., Roux,P.F., Tabet,I., Laguesse,S.,
            Florido,J., Houdeville,M., Choquet,A., Encislai,B., Arena,G., De
            Blasio,C., Wendling,O., Frenois,F.X., Papon,L., Stuani,L.,
            Fuentes,M., Jahannault Talignani,C., Rousseau,M., Guegan,J.,
            Buscail,Y., Dupre,P., Michaud,H.A., Rodier,G., Bellvert,F.,
            Kulyk,H., Ferraro Peyret,C., Mathieu,H., Close,P., Rapino,F.,
            Chaveroux,C., Pirot,N., Rubio,L., Torro,A., Sorg,T., Ango,F.,
            Hirtz,C., Compan,V., Lebigot,E., Legati,A., Ghezzi,D., Nguyen,L.,
            David,A., Sardet,C., Lacroix,M. and Le Cam,L.
  TITLE     E4F1 coordinates pyruvate metabolism and the activity of the
            elongator complex to ensure translation fidelity during brain
            development
  JOURNAL   Nat Commun 16 (1), 67 (2025)
   PUBMED   39747033
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Laguesse,S., Creppe,C., Nedialkova,D.D., Prevot,P.P., Borgs,L.,
            Huysseune,S., Franco,B., Duysens,G., Krusy,N., Lee,G., Thelen,N.,
            Thiry,M., Close,P., Chariot,A., Malgrange,B., Leidel,S.A.,
            Godin,J.D. and Nguyen,L.
  TITLE     A Dynamic Unfolded Protein Response Contributes to the Control of
            Cortical Neurogenesis
  JOURNAL   Dev Cell 35 (5), 553-567 (2015)
   PUBMED   26651292
REFERENCE   3  (bases 1 to 3236)
  AUTHORS   Mohammad,F., Mondal,T., Guseva,N., Pandey,G.K. and Kanduri,C.
  TITLE     Kcnq1ot1 noncoding RNA mediates transcriptional gene silencing by
            interacting with Dnmt1
  JOURNAL   Development 137 (15), 2493-2499 (2010)
   PUBMED   20573698
REFERENCE   4  (bases 1 to 3236)
  AUTHORS   Furuya,S., Yoshida,K., Kawakami,Y., Yang,J.H., Sayano,T., Azuma,N.,
            Tanaka,H., Kuhara,S. and Hirabayashi,Y.
  TITLE     Inactivation of the 3-phosphoglycerate dehydrogenase gene in mice:
            changes in gene expression and associated regulatory networks
            resulting from serine deficiency
  JOURNAL   Funct Integr Genomics 8 (3), 235-249 (2008)
   PUBMED   18228065
REFERENCE   5  (bases 1 to 3236)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   6  (bases 1 to 3236)
  AUTHORS   Engemann,S., Strodicke,M., Paulsen,M., Franck,O., Reinhardt,R.,
            Lane,N., Reik,W. and Walter,J.
  TITLE     Sequence and functional comparison in the Beckwith-Wiedemann
            region: implications for a novel imprinting centre and extended
            imprinting
  JOURNAL   Hum Mol Genet 9 (18), 2691-2706 (2000)
   PUBMED   11063728
REFERENCE   7  (bases 1 to 3236)
  AUTHORS   Yatsuki,H., Watanabe,H., Hattori,M., Joh,K., Soejima,H., Komoda,H.,
            Xin,Z., Zhu,X., Higashimoto,K., Nishimura,M., Kuratomi,S.,
            Sasaki,H., Sakaki,Y. and Mukai,T.
  TITLE     Sequence-based structural features between Kvlqt1 and Tapa1 on
            mouse chromosome 7F4/F5 corresponding to the Beckwith-Wiedemann
            syndrome region on human 11p15.5: long-stretches of unusually well
            conserved intronic sequences of kvlqt1 between mouse and human
  JOURNAL   DNA Res 7 (3), 195-206 (2000)
   PUBMED   10907850
REFERENCE   8  (bases 1 to 3236)
  AUTHORS   Kato,R., Shirohzu,H., Yokomine,T., Mizuno,S., Mukai,T. and
            Sasaki,H.
  TITLE     Sequence-ready 1-Mb YAC, BAC and cosmid contigs covering the distal
            imprinted region of mouse chromosome 7
  JOURNAL   DNA Res 6 (6), 401-405 (1999)
   PUBMED   10691133
REFERENCE   9  (bases 1 to 3236)
  AUTHORS   Zimmer,Y., Milo-Landesman,D., Svetlanov,A. and Efrat,S.
  TITLE     Genes induced by growth arrest in a pancreatic beta cell line:
            identification by analysis of cDNA arrays
  JOURNAL   FEBS Lett 457 (1), 65-70 (1999)
   PUBMED   10486565
REFERENCE   10 (bases 1 to 3236)
  AUTHORS   Sowden,J., Smith,H., Morrison,K. and Edwards,Y.
  TITLE     Sequence comparisons and functional studies of the proximal
            promoter of the carbonic anhydrase 3 (CA3) gene
  JOURNAL   Gene 214 (1-2), 157-165 (1998)
   PUBMED   9651514
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY253195.1, BY021374.1, AK135433.1, AJ276796.1 and BC030473.1.
            
            On Nov 19, 2011 this sequence version replaced NM_013742.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and it encodes the longer protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK033328.1, AK135433.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 BY253195.1         2-5
            5-31                BY021374.1         1-27
            32-2152             AK135433.1         2-2122
            2153-2747           AJ276796.1         2120-2714
            2748-3236           BC030473.1         2184-2672
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 88.25 cM"
     gene            1..3236
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /note="cysteinyl-tRNA synthetase 1"
                     /db_xref="GeneID:27267"
                     /db_xref="MGI:MGI:1351477"
     exon            1..109
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..2580
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /EC_number="6.1.1.16"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cysteinyl-tRNA synthetase, cytoplasmic; cysRS;
                     cysteine--tRNA ligase, cytoplasmic"
                     /codon_start=1
                     /product="cysteine--tRNA ligase, cytoplasmic isoform 1"
                     /protein_id="NP_038770.3"
                     /db_xref="CCDS:CCDS40198.1"
                     /db_xref="GeneID:27267"
                     /db_xref="MGI:MGI:1351477"
                     /translation="MAGSSAEQAADYRSILSISDEAARVQALDQHLSTRSYIQGYSLS
                     QADVDVFRQLSAPPADSRLFHVARWFRHIEALLGGPQGRDEPCRLQASKGRRVQPQWS
                     PPAGTEPCRLRLYNSLTRNKDVFIPQDGKKVTWYCCGPTVYDASHMGHARSYISFDIL
                     RRVLRDYFQYDVFYCMNITDIDDKIIRRARQNYLFEQYREQKPPATQLLKDVRDAMKP
                     FSVKLSETTDPDKRQMLERIQNSVKLATEPLEQAVRSSLSGEEVDSKVQVLLEEAKDL
                     LSDWLDSTGGSEVTDNSIFSKLPKFWEEEFHKDMEALNVLPPDVLTRVSEYVPEIVNF
                     VQKIVDNGYGYASNGSVYFDTAKFAASEKHSYGKLVPEAVGDQKALQEGEGDLSISAD
                     RLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASMDIHGGGF
                     DLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHS
                     ARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFMNEFFLNVKDILRAPVDITGQFEKW
                     EAEEVELNKNFYGKKTAVHEALCDNIDTRTVMEEMRALVSQCNLYMAARKAERRRPNR
                     ALLENIAMYLTHMLKIFGAIEEESPLGFPVGGPGTNLNLESTVMPYLQVLSEFREGVR
                     KIAREKKVLEVLQLSDALRDDILPELGVRFEDHEGLPTVVKLVDRDTLLKEKEGKKRA
                     EEEKRRKKEEAARKKQEQEAAKLAKMKIPPSEMFLSEVNKYSKFDENGLPTHDTEGKE
                     LSKGQAKKLKKLFEAQEKLYKEYLQMLQNGSLQ"
     exon            110..358
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            359..450
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            451..539
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            540..636
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            637..735
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            736..885
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            886..1026
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1115
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1237
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1350
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1479
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1609
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1713
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1811
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..2001
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2070
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..2152
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2233
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            2234..2301
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            2302..2361
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            2362..2445
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..3222
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2726..2731
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /note="hexamer: AATAAA"
     polyA_site      2747
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /note="major polyA site"
     regulatory      3131..3136
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
                     /note="hexamer: GATAAA"
     polyA_site      3152
                     /gene="Cars1"
                     /gene_synonym="CA3; Cars"
ORIGIN      
        1 ggtggggcgg gacttccggc gccgcggttg catcagattc tggaaggcgt ctgtggcggc
       61 ggccgcgggt cttgattccc agtgatggca ggttcctccg cggagcaggc tgccgactac
      121 aggtccattc tgagcattag tgacgaggca gccagggtgc aggccctgga ccaacacctc
      181 agcacgcgta gctatatcca ggggtactcc ctatcccagg cagatgtgga cgtgttcagg
      241 cagctctcgg cccctcccgc tgactcaagg ctcttccacg tggctcggtg gttcaggcac
      301 atagaagcgc tcctgggtgg cccccaaggc agagacgagc cctgcaggct tcaagcaagt
      361 aaaggccggc gagtgcagcc tcagtggtcc cccccagcag ggactgagcc ctgcaggctc
      421 cgcctctata atagcctcac ccggaacaag gatgtattta tacctcaaga tgggaagaag
      481 gtgacgtggt actgttgcgg gccgactgtc tatgatgcgt ctcacatggg acatgccagg
      541 tcctacatct ccttcgatat cctgaggagg gtgctgaggg attacttcca gtatgatgtc
      601 ttttactgca tgaacatcac agacattgat gataagatca tcaggcgggc acggcagaac
      661 tacctgtttg agcagtatcg ggagcagaaa cccccagcca cccagctcct gaaggatgtc
      721 cgtgatgcca tgaagccatt ttcagtcaag ttaagtgaga caacagatcc cgacaagagg
      781 cagatgctgg agcggatcca gaactctgtg aaacttgcca cagaaccact ggaacaggct
      841 gtgcgatcca gcctctctgg agaggaagta gacagcaaag tacaggtgtt gctggaggaa
      901 gcaaaggact tgctctctga ctggctggat tccacaggtg gcagtgaggt gactgacaac
      961 tcgattttct ccaaactgcc caagttctgg gaagaagagt tccacaaaga catggaagcc
     1021 ctgaatgttc tccctcctga tgtcctaacc cgtgtcagtg aatatgtgcc agaaattgtg
     1081 aacttcgtcc agaagattgt ggacaatggt tatggttatg cttcaaatgg atctgtttac
     1141 ttcgacacag ccaagtttgc tgctagtgaa aagcactcct atgggaagct ggtgcctgag
     1201 gccgttgggg atcagaaggc acttcaggaa ggggaaggtg atctgagcat ctctgctgac
     1261 cgccttagtg agaaacgctc tcccaatgac tttgccttgt ggaaggcctc caagccaggc
     1321 gagccatcct ggccttgccc ctggggaaag ggtcgtcccg gatggcacat agagtgttct
     1381 gctatggcag gcacgctcct gggagcctca atggacattc atggtggagg gtttgacctc
     1441 cgcttccccc accatgacaa tgagctggca cagtcggagg cctactttga aaatgactgc
     1501 tgggtcaggt acttcttgca cacgggccac ttgacgatag caggctgcaa gatgtccaag
     1561 tcactgaaaa actttatcac cattaaagat gccttgaaga agcactcagc acggcagctg
     1621 cgactggcat tcctcatgca ctcatggaaa gacacactgg actattccag caacactatg
     1681 gagtctgctc ttcagtatga aaaattcatg aacgagtttt tcttaaatgt gaaagacatc
     1741 ctccgagccc ctgtagacat cactggccag tttgaaaagt gggaagctga agaagtggag
     1801 ctaaataaga acttctatgg caagaagacc gcagttcacg aagccttgtg tgataacatt
     1861 gacacccgga ctgtcatgga agagatgcgg gctttggtca gtcagtgcaa cctctacatg
     1921 gcagccagga aggctgaacg gaggaggccc aaccgggctc tgctggagaa cattgccatg
     1981 tacctcaccc acatgctgaa gatctttggg gccatagagg aggagagccc cctggggttc
     2041 ccagttggtg ggcctggaac caacctgaac ctcgagtcaa cagtcatgcc ctaccttcag
     2101 gtgttatcag aattcagaga gggagtacga aagattgccc gagagaaaaa agttcttgag
     2161 gttctacagc tcagtgatgc cctccgggat gacatcctgc ctgagcttgg ggtccggttt
     2221 gaagaccatg aagggctgcc aacagtggtg aagttggtgg acagagacac cttactgaaa
     2281 gagaaggaag gaaagaaaag ggctgaagaa gagaagagga ggaagaaaga ggaggcggcc
     2341 agaaagaaac aggagcaaga agcagcaaag ctggccaaga tgaagatacc acccagtgag
     2401 atgttcctgt cagaagtcaa caagtattct aaatttgatg aaaatggtct gcctacccac
     2461 gacaccgaag gcaaagagct gagcaagggc caggccaaga agctgaagaa gctctttgaa
     2521 gcccaggaga aactgtacaa ggagtatctg caaatgttac agaatggcag cctccagtga
     2581 tgccacggca gcccaggagg ggcgcacctg ccagcctggc tgctatggac taggccacag
     2641 ctactgaggc tccgtgccct gatcatgttt acagctgttc tcacacctga atcaggcaga
     2701 gtggtagcag ctctgggaca ctgataataa attcttctga acggtgaggt ctggtctgcc
     2761 ctctcagcac ttggagagct gacctacagg cgcctggtca gcagcttgca acttaggcct
     2821 cctagtccat tgagcccctt cagccagcct gcccgaggaa tttccctcac attggcaagc
     2881 tgagctaatc tctgctgtgt tggcacattt ggtggtattg gagtagggaa aaggccgact
     2941 cagcctaacg gatccccctg agtcccagac aaaagccact cagttggctg tctggctctg
     3001 atgagcacac actgcgggtt tcccagagca actggccagc aggaccgttg ctgtgtgggg
     3061 tggggctctg gcaagctgcc tgggactgcc gttatgacag caagcccagt tcagaaccac
     3121 tggcttgtaa gataaagtgt gtctaggtcc aacccaagtg tgttcctgtc cacctgctcc
     3181 tgcctgtttg ctctcagccc tgctaagaat cagcacctaa gaaaaaaaaa aaaaaa
//
