LOCUS       NM_026825               5158 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus capping protein regulator and myosin 1 linker 1
            (Carmil1), transcript variant 1, mRNA.
ACCESSION   NM_026825 XM_488514 XM_488536
VERSION     NM_026825.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5158)
  AUTHORS   Jo,Y.J., Jang,W.I., Namgoong,S. and Kim,N.H.
  TITLE     Actin-capping proteins play essential roles in the asymmetric
            division of maturing mouse oocytes
  JOURNAL   J Cell Sci 128 (1), 160-170 (2015)
   PUBMED   25395583
  REMARK    GeneRIF: Expression of the capping-protein-binding region of CARMIL
            (also known as LRRC16A) impaired spindle migration and polar body
            extrusion during oocyte maturation and decreased the density of the
            cytoplasmic actin mesh
REFERENCE   4  (bases 1 to 5158)
  AUTHORS   Fujiwara,I., Remmert,K., Piszczek,G. and Hammer,J.A.
  TITLE     Capping protein regulatory cycle driven by CARMIL and V-1 may
            promote actin network assembly at protruding edges
  JOURNAL   Proc Natl Acad Sci U S A 111 (19), E1970-E1979 (2014)
   PUBMED   24778263
  REMARK    GeneRIF: Our data argue that a large pool of freely diffusing,
            inactive capping protein (CP:V-1) feeds, via CARMIL-driven complex
            exchange, the formation of weak-capping complexes (CP:CARMIL) at
            the plasma membrane of protruding edges.
REFERENCE   5  (bases 1 to 5158)
  AUTHORS   Adissu,H.A., Estabel,J., Sunter,D., Tuck,E., Hooks,Y.,
            Carragher,D.M., Clarke,K., Karp,N.A., Newbigging,S., Jones,N.,
            Morikawa,L., White,J.K. and McKerlie,C.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Histopathology reveals correlative and unique phenotypes in a
            high-throughput mouse phenotyping screen
  JOURNAL   Dis Model Mech 7 (5), 515-524 (2014)
   PUBMED   24652767
REFERENCE   6  (bases 1 to 5158)
  AUTHORS   Takeda,S., Minakata,S., Koike,R., Kawahata,I., Narita,A.,
            Kitazawa,M., Ota,M., Yamakuni,T., Maeda,Y. and Nitanai,Y.
  TITLE     Two distinct mechanisms for actin capping protein
            regulation--steric and allosteric inhibition
  JOURNAL   PLoS Biol 8 (7), e1000416 (2010)
   PUBMED   20625546
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5158)
  AUTHORS   Fujiwara,I., Remmert,K. and Hammer,J.A. 3rd.
  TITLE     Direct observation of the uncapping of capping protein-capped actin
            filaments by CARMIL homology domain 3
  JOURNAL   J Biol Chem 285 (4), 2707-2720 (2010)
   PUBMED   19926785
  REMARK    GeneRIF: the isolated CAH3 domain of CARMIL (and presumably the
            intact molecule as well) possesses the ability to uncap CP-capped
            actin filaments
REFERENCE   8  (bases 1 to 5158)
  AUTHORS   Bailey,P.J., Klos,J.M., Andersson,E., Karlen,M., Kallstrom,M.,
            Ponjavic,J., Muhr,J., Lenhard,B., Sandelin,A. and Ericson,J.
  TITLE     A global genomic transcriptional code associated with CNS-expressed
            genes
  JOURNAL   Exp Cell Res 312 (16), 3108-3119 (2006)
   PUBMED   16919269
REFERENCE   9  (bases 1 to 5158)
  AUTHORS   Yang,C., Pring,M., Wear,M.A., Huang,M., Cooper,J.A., Svitkina,T.M.
            and Zigmond,S.H.
  TITLE     Mammalian CARMIL inhibits actin filament capping by capping protein
  JOURNAL   Dev Cell 9 (2), 209-221 (2005)
   PUBMED   16054028
  REMARK    GeneRIF: mCARMIL is a key regulator of CP and has profound effects
            on cell behavior.
REFERENCE   10 (bases 1 to 5158)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK051570.1, AY437876.1, AK043591.1 and AK004138.1.
            
            On Jul 28, 2007 this sequence version replaced NM_026825.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY437876.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-969               AK051570.1         1-969
            970-2196            AY437876.1         975-2201
            2197-2944           AK043591.1         777-1524
            2945-3117           AY437876.1         2950-3122
            3118-4181           AK043591.1         1716-2779
            4182-5158           AK004138.1         209-1185
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 10.05 cM"
     gene            1..5158
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /note="capping protein regulator and myosin 1 linker 1"
                     /db_xref="GeneID:68732"
                     /db_xref="MGI:MGI:1915982"
     exon            1..230
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     CDS             191..4315
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /note="isoform 1 is encoded by transcript variant 1;
                     leucine rich repeat containing 16; leucine-rich
                     repeat-containing protein 16A; CARMIL homolog; capping
                     protein, Arp2/3 and myosin-I linker homolog 1; capping
                     protein, Arp2/3 and myosin-I linker protein 1;
                     F-actin-uncapping protein LRRC16A; leucine rich repeat
                     containing 16A; capping protein regulator and myosin 1
                     linker protein 1"
                     /codon_start=1
                     /product="F-actin-uncapping protein LRRC16A isoform 1"
                     /protein_id="NP_081101.3"
                     /db_xref="CCDS:CCDS36621.1"
                     /db_xref="GeneID:68732"
                     /db_xref="MGI:MGI:1915982"
                     /translation="MTDESSDVPRELMESIKDVIGRKIKISVKKKVKLEVKGDRVENK
                     VLVLTSCRAFLLSARIPSKLELTFSYLEIHGVICHKPAQMVVETEKCNMSMKMVSPED
                     VSEVLAHIGTCLRRIFPGLSPLRIMKKVSMEPSERLASLQALWDSQTLAEPGPCGGFS
                     QMYACVCDWLGFSYKEEVQWDVDTIYLTQDTRELNLQDFSHLEHRDLIPIIAALEYNQ
                     WFTKLSSKDLKLSTDVCEQILRVVSRSNRLEELVLENAGLRIDFAQKLAGALAHNPNS
                     GLHTINLAGNSLEDRGVSSLSIQFAKLPKGLKHLNLSKTSLSPKGVNSLCQSLSANPL
                     TASTLTHLDLSGNALRGDDLSHMYNFLAQPNTIVHLDLSNTECSLEMVCSALLRGCLQ
                     CLAVLNLSRSVFSHRKGKEVPPSFKQFFSSSLALIQINLSGTKLSPEPLKALLLGLAC
                     NHSLKGVSLDLSNCELGHCLRSGGAQVLEGCIAEIHNITSLDISDNGLESDLSTLIVW
                     LSKNRSIQHLALGKNFNNMKSKNLTPVLDNLVQMIQDEDSPLQSLSLADSKLKAEVTI
                     IINALGSNTSLTKVDISGNGMGDMGAKMLAKALQINTKLRTVIWDKNNITAQGFQDIA
                     VAMEKNYTLRFMPIPMYDAAQALKTNPEKTEEALQKIENYLLRNHETRKYLQEQAYRL
                     QQGIVTSTTQQMIDRICVKVQDHLNSLRACGGDAIQEDLKAAERLMRDAKNSKTLLPN
                     LYHVGGASWAGASGLSSSPIQETLESMAGEVTRVVDEQLKDLLESMVDAAETLCPNVM
                     RKAHIRQDLIHASTEKISIPRTFVKNVLLEQSGIDILNKISEVKLTVASFLSDRIVDE
                     ILDSLSSSHRKLANHFSRLNKSLPQREDLEVELVEEKPVKRAILTVEDLTEVERLEDL
                     DTCMMTPKSKRKSIHSRMLRPVSRAFEMEFDLDKALEEVPIHIEDPPFPSVRQEKRSS
                     GLISELPSEEGRRLEHFTKLRPKRNKKQQPTQAAVCTISILPQDGEQNGLMGRVDEGV
                     DEFFTKKVTKMDCKRSSSRSSDAHELGEGDEKKKRDSRRSGFLNLIKSRSRSERPPTV
                     LMTEELSSPKGAMRSPPVDTTRKEIKAAEHNGAPDRTEEIKTPEPLEEGPAEEAGRAE
                     RSDSRGSPQGGRRYVQVMGSGLLAEMKAKQERRAACAQKKLGNDVISQDPSSPVSCNT
                     ERLEGGATVPKLQPGLPEARFGSGTPEKNAKAEPRVDGGCRSRSSSSMPTSPKPLLQS
                     PKPSPSARPSIPQKPRTASRPEDTPDSPSGPSSPKVALLPPILKKVSSDKERDGQNSS
                     QSSPRSFSQEASRRSWGPAQEYQEQKQRSSGKDGHQGSKCSDSGEEAEKEFIFV"
     exon            231..328
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            329..379
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            380..439
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            440..561
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            562..659
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            660..730
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            731..804
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            805..880
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            881..969
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1064
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1151
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1255
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1333
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1410
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1515
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1585
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1597
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1679
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..1779
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1834
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            1835..2007
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2008..2076
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2077..2170
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2269
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2270..2398
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2399..2530
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2531..2706
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2707..2794
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2795..2944
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            2945..3011
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            3012..3208
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            3209..3321
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            3322..3745
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            3746..3827
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            3828..4040
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            4041..4181
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     exon            4182..5158
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5138..5143
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /note="putative"
     polyA_site      5158
                     /gene="Carmil1"
                     /gene_synonym="1110037D04Rik; CARMIL; CARML1; D130057M20;
                     Lrrc16; Lrrc16a"
                     /note="putative"
ORIGIN      
        1 tttttttttt ttttttttgg tactgcgggc gcgcggcaac cttggatcaa cgccccgctg
       61 ctccggccgg cccactcttt ccttgcaagc tgcggcttcc tcgcggaaac attaaagggt
      121 ctgaagaagg gcgcggggcc gagagtcaga gagtgggacc cgggatctcc ccgcgcccag
      181 tggggcaacc atgaccgacg agagctccga tgtcccccgg gagttaatgg aaagcatcaa
      241 ggatgttatt ggcaggaaga tcaaaatctc agtgaagaag aaagtgaagt tggaagtgaa
      301 gggcgacagg gtggaaaaca aagtgctggt gctcacatca tgccgagcct tcctcctgtc
      361 agcacgcatc cccagcaagc tcgagttaac cttcagttat ctagagattc atggagtcat
      421 ctgccacaag ccagcccaga tggttgtgga gactgagaag tgtaacatgt ccatgaagat
      481 ggtgtcccca gaggatgtga gtgaagtgct ggctcacata ggcacctgct tgcggaggat
      541 attcccaggc ctttccccac tgagaatcat gaagaaagtc tccatggagc catctgaacg
      601 tctggccagt ctccaggccc tgtgggacag ccagaccttg gctgagccag gcccctgtgg
      661 tgggttttct cagatgtatg cctgtgtgtg cgactggctg ggcttttcat acaaggaaga
      721 agtgcagtgg gatgtggata caatttatct gacacaagac accagggaat taaatttaca
      781 ggacttcagt catctcgagc acagagacct gatccccatc attgctgccc tggagtataa
      841 ccagtggttc accaaactgt cctctaagga tctcaaactg tccacggatg tttgtgaaca
      901 gattttgaga gtggtgagta ggtccaatcg actggaagag ttggtgttgg aaaatgctgg
      961 actcagaata gattttgcac agaaattggc cggtgctcta gcacacaatc ctaactcagg
     1021 acttcacaca atcaaccttg ctgggaactc gctggaggat cgaggtgtgt cctccttaag
     1081 tattcaattt gccaaactcc caaagggatt gaagcatttg aacctctcta aaacctcatt
     1141 gtcacctaaa ggggtgaaca gcctttgtca gtccctcagt gccaaccctc tgactgcctc
     1201 taccctcacc cacctggacc tctcagggaa tgccctccgc ggagatgacc tctcgcacat
     1261 gtacaatttt ttggcccagc ccaacaccat tgttcatctg gatctatcta atacagaatg
     1321 ttctctggag atggtgtgta gcgctctcct ccggggttgc cttcagtgtc tcgctgtgct
     1381 caatctctcc agatctgtct tctctcacag gaaaggaaag gaagtccctc cgtctttcaa
     1441 gcagtttttc agcagttctc tggctttgat ccaaatcaac ctttcaggca cgaagctgtc
     1501 tcccgagccc ttgaaagcgc tgctcttggg cctggcctgc aatcatagcc tgaagggagt
     1561 ctctctggat ctcagcaact gtgagcttgg ccactgtctg aggtcaggag gagctcaagt
     1621 gttagaaggc tgcatcgctg aaatccacaa catcaccagc ttagatatct ctgacaatgg
     1681 tttagaatcc gacctatcta ccctaattgt gtggctcagt aaaaacagat caatacagca
     1741 cctggcattg ggcaaaaatt ttaataatat gaaatccaaa aatctgacgc ctgtgttgga
     1801 caacttagtg cagatgatcc aagatgaaga ctctcctctg cagtcgttgt ccctggctga
     1861 ctcaaaactc aaggctgagg tcaccatcat catcaatgcc ctgggcagca acacctccct
     1921 gaccaaagtg gacatcagtg ggaatggcat gggggacatg ggagcgaaga tgctggccaa
     1981 ggctctccag atcaacacca aactcaggac agtcatctgg gacaaaaaca acatcactgc
     2041 acaaggcttt caagacatag ctgttgctat ggaaaagaac tacacactgc gatttatgcc
     2101 aattccgatg tatgatgccg ctcaagctct caaaaccaac cccgagaaaa cagaagaggc
     2161 tctgcagaag atagaaaatt atctgctccg gaatcacgag accaggaaat accttcagga
     2221 gcaggcctat cgcctccagc agggcatcgt caccagcacc acccagcaga tgattgaccg
     2281 gatatgtgtg aaggttcagg atcacctcaa ctccttacga gcctgtgggg gtgatgctat
     2341 tcaggaggat ttaaaggcag cggagcggct catgcgagat gctaagaact ctaaaacatt
     2401 gttgcccaat ttatatcatg tgggcggtgc atcctgggca ggagccagtg ggctgtcatc
     2461 cagtcccatt caggagaccc tggagtcgat ggctggagaa gtcacaagag tggtggatga
     2521 acaactgaag gatttgctag aatccatggt ggacgcagcc gagaccctct gccccaacgt
     2581 catgagaaaa gcccacatcc gacaggacct gatccatgcc agcaccgaaa agatttccat
     2641 cccacgtacc tttgttaaaa atgtcctgtt ggaacagtcc gggattgaca ttcttaacaa
     2701 aatcagtgag gtgaagctga cagtggcctc gttcttgtct gaccggatcg tcgatgagat
     2761 cctggattct ctctccagca gccatcggaa gctggccaac catttcagca ggctaaacaa
     2821 gagccttccc cagcgagagg acctagaggt ggagctggtg gaggagaagc cggtgaaacg
     2881 ggccatcctc acggtagagg atctcacaga ggtggagagg ctggaagacc tagacacttg
     2941 tatgatgact cctaagtcca agaggaagag catccacagc cgaatgctgc ggcctgtctc
     3001 cagggccttc gaaatggagt ttgatcttga taaagccctg gaagaggtgc cgattcacat
     3061 cgaagaccca cctttcccat cagtccggca agagaagcga agctcggggt taatctctga
     3121 gctgccctcg gaggaaggca ggagactgga acacttcacc aagttaaggc ccaagcggaa
     3181 caagaagcag caacctacgc aagcagcggt ctgtaccatc agcatcttgc cccaggatgg
     3241 agaacagaat ggcctcatgg gcagagtgga tgaaggggta gatgagtttt tcaccaagaa
     3301 ggtgactaaa atggattgca agaggtcatc ctcgcgctcc tcagatgccc acgaactcgg
     3361 ggaaggagat gagaagaaaa aacgggactc tcggaggagt ggcttcctca acctgattaa
     3421 atccaggtcc agatctgagc ggccacccac agtcctgatg acggaagagc tctcctcccc
     3481 gaaaggggca atgcggagcc caccagtgga taccaccagg aaggagataa aggcagcgga
     3541 gcacaatggt gctccagacc gcacagaaga gattaagaca ccggaacccc tggaggaggg
     3601 tccagcagag gaagctggca gggctgagcg cagtgacagc aggggcagcc cgcagggtgg
     3661 ccggcgctat gtgcaggtga tgggcagcgg cctgctggcc gagatgaagg ccaagcagga
     3721 gcggagggca gcatgtgcac agaagaagct cggcaatgat gtcatctccc aggatccttc
     3781 cagcccagtc tcgtgcaaca cagagcgttt agaaggaggg gcgacagtgc ctaaactgca
     3841 accaggtctt ccagaggccc gctttggttc gggaacacca gaaaagaatg ccaaagctga
     3901 acccagagtg gatggaggct gcaggtcccg gagctcctcc agcatgccca ccagcccaaa
     3961 gcccctcctt cagtccccta agcccagccc ttcagcccgg ccttctatcc ctcagaagcc
     4021 aagaactgcc tcccgacctg aagacacccc agactctcca tctggtccta gttcccctaa
     4081 agttgccctc cttccaccca tcctcaaaaa agtctcctcg gacaaggagc gtgacggcca
     4141 gaacagctca cagtccagtc ctaggagctt ctcccaggaa gcctcaagga gaagctgggg
     4201 cccggcccag gagtatcaag aacagaagca acggtcctcg ggcaaagatg gtcaccaagg
     4261 gagcaaatgc agtgactctg gagaagaggc agaaaaagag tttatttttg tgtaatggtg
     4321 cccaccccaa gcctctccat gcagggcgct ctcctcagct ctcagagagg agccagggcc
     4381 agtacctctt ctctcttctc ttggtgcttt cttctttctc tcctttcttc tttctcattt
     4441 atatatattt tcaaattttt ggtcttcccc cattcttggc atttgatttc taaacactgt
     4501 ttcttatgcc ttcaacaaaa agccagcaat tattccatgg gccctacttg aatttctctg
     4561 aggcagctac acattgccct gcatggtgag ttgtttttgg aaattaatga agtaactgga
     4621 ctcacaccca agtaacaatc cgggaggtcc tggggtgcta cagggtgtgt ttgtgtcaga
     4681 tccacacata actcgataga gcaatttatt cttgatgtat gcaattgcac attgtaatta
     4741 tattaacaga gcacactaat aaataatttg tataaattat aaatattaga tcttgggcac
     4801 agttcttaca ttcttttgtg ggacatttcc ttccccatgt agaattgtac atttaaacct
     4861 tggttggtaa tatttgcaga ccatcggcaa gcacagctaa aaacagtgtg agaagctcag
     4921 attcatgtat atacttgatt ggaatgaggt cttataaata ttcatgtttc tgaaggcctt
     4981 taatttctgt ctgcttatta gtttttaatg tgtggttttt aaaagaaaat tttggcacct
     5041 gtaaaaaaaa tctaaatact actctttata aaacatctca caaatattca cttacattac
     5101 aagctgaaag tattttattc atatgtatat ttataccaat aaaataattt tacaagtg
//
