LOCUS       NM_139150               5574 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus calcium response factor (Carf), transcript variant 1,
            mRNA.
ACCESSION   NM_139150
VERSION     NM_139150.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. PMID: 29144450]
REFERENCE   3  (bases 1 to 5574)
  AUTHORS   Lyons,M.R., Chen,L.F., Deng,J.V., Finn,C., Pfenning,A.R.,
            Sabhlok,A., Wilson,K.M. and West,A.E.
  TITLE     The transcription factor calcium-response factor limits NMDA
            receptor-dependent transcription in the developing brain
  JOURNAL   J Neurochem 137 (2), 164-176 (2016)
   PUBMED   26826701
  REMARK    GeneRIF: This report that loss of CaRF leads to enhanced
            NMDAR-dependent induction of both Bdnf IV and the
            activity-regulated gene Arc, and we investigate potential
            mechanisms as well as biological consequences of this change in
            transcriptional regulation.
REFERENCE   4  (bases 1 to 5574)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 5574)
  AUTHORS   Agostino,P.V., Cheng,R.K., Williams,C.L., West,A.E. and Meck,W.H.
  TITLE     Acquisition of response thresholds for timed performance is
            regulated by a calcium-responsive transcription factor, CaRF
  JOURNAL   Genes Brain Behav 12 (6), 633-644 (2013)
   PUBMED   23848551
  REMARK    GeneRIF: Carf(+/-) and Carf(-/-) mice were impaired in their
            ability to acquire timed response thresholds relative to Carf(+/+)
            mice.
REFERENCE   6  (bases 1 to 5574)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   7  (bases 1 to 5574)
  AUTHORS   Cheung,C.T., Hasan,M.K., Widodo,N., Kaul,S.C. and Wadhwa,R.
  TITLE     CARF: an emerging regulator of p53 tumor suppressor and senescence
            pathway
  JOURNAL   Mech Ageing Dev 130 (1-2), 18-23 (2009)
   PUBMED   18555516
  REMARK    Review article
REFERENCE   8  (bases 1 to 5574)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 5574)
  AUTHORS   Hasan,M.K., Yaguchi,T., Sugihara,T., Kumar,P.K., Taira,K.,
            Reddel,R.R., Kaul,S.C. and Wadhwa,R.
  TITLE     CARF is a novel protein that cooperates with mouse p19ARF (human
            p14ARF) in activating p53
  JOURNAL   J Biol Chem 277 (40), 37765-37770 (2002)
   PUBMED   12154087
REFERENCE   10 (bases 1 to 5574)
  AUTHORS   Tao,X., West,A.E., Chen,W.G., Corfas,G. and Greenberg,M.E.
  TITLE     A calcium-responsive transcription factor, CaRF, that regulates
            neuronal activity-dependent expression of BDNF
  JOURNAL   Neuron 33 (3), 383-395 (2002)
   PUBMED   11832226
  REMARK    GeneRIF: Cloned as a calcium- and neural-selective transcriptional
            activator of the gene for Brain-Derived Neurotrophic Factor (BDNF).
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK044738.1, AF454947.1, AC138597.7 and BF456537.1.
            
            [WARNING] On Jul 23, 2024 this sequence was replaced by
            NM_139150.6.
            
            On Oct 22, 2013 this sequence version replaced NM_139150.4.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219381.278431.1, AF454947.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AK044738.1         2-35
            35-2472             AF454947.1         4-2441
            2473-2474           AC138597.7         5932-5933           c
            2475-2579           AF454947.1         2442-2546
            2580-2580           AC138597.7         5826-5826           c
            2581-2636           AF454947.1         2548-2603
            2637-5247           AC138597.7         3159-5769           c
            5248-5574           BF456537.1         1-327               c
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 30.45 cM"
     gene            1..5574
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /note="calcium response factor"
                     /db_xref="GeneID:241066"
                     /db_xref="MGI:MGI:2182269"
     exon            1..61
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            62..178
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            179..296
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             222..2291
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     amyotrophic lateral sclerosis 2 chromosomal region
                     candidate gene 8 protein homolog; calcium-response factor
                     CaRF; calcium-responsive transcription factor"
                     /codon_start=1
                     /product="calcium-responsive transcription factor isoform
                     1"
                     /protein_id="NP_631889.1"
                     /db_xref="CCDS:CCDS14989.1"
                     /db_xref="GeneID:241066"
                     /db_xref="MGI:MGI:2182269"
                     /translation="MEQRHSLAGNCDDGEKSEREAQGFEHRTCMDSGDPSFGQNDPPT
                     ILPITAPKTYDSLTSQDMPETLTDTQALHEEQFHLLDQNGQPIQYDLQSLGNSTAQMT
                     GMEQVAIPQEPFADEHSPQDISEEKPTGVRADVLEDSTSNYGLRSPASLVLPKKAGAR
                     LVEEPLLAPLQPLSCNTPMWACRLRSCEKIGDSYRGYCVSETELESVLTFHKQQTQTV
                     WGTRQSPSPAKPATRLMWKSQYVPYDGIPFVNAGSRAVVMECQYGPRRKGFQLKKISE
                     QESRSCHLYKATCPARIYIKKVQKFPEYRVPTDPQIDRKIIRLEQEKAFTMLKKNLMD
                     AGGVLRWYVQLPTQQAHQYHESETPGVPLSPSPFPMSPLEEEEAIVRDENCALPSRLH
                     PQVAHKIQELVSQGVGQVYAVRKQLRKFVERELFKPDEIPERHNLSYFPTVNDIKNHI
                     HEVQKSLRTGDVVYNSEIIPATLQWTTDSGNILRETVTVTFAEGNLLGEPIPSKMGTS
                     QTQTAVSPEPLSSFPPKIFTHFQALKLQPRLSSPDGSQALVSVDSHASSSPPGLVDTV
                     GNAEVDNHSVLLGQSQNPGTDTCLTQDNSTSSSTGHLPESVPNPVAEDQLLEGEDVED
                     AGNPEGSVNRTLLGDVQTVPIQIIDSRPVLVEESLSKNQVKQETNEPTLSTEAKTFLD
                     CKKISAT"
     exon            297..524
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            525..585
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            586..700
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            701..785
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            786..975
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1096
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1240
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1474
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1637
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1638..1701
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1814
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1815..2184
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2185..5557
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2617..2622
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /note="hexamer: AATAAA"
     polyA_site      2639
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
     regulatory      5533..5538
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /note="hexamer: ATTAAA"
     polyA_site      5557
                     /gene="Carf"
                     /gene_synonym="Als2cr8; ECBRC-FC1; ECBRC-FC2"
                     /note="major polyA site"
ORIGIN      
        1 aggaggggtg cgcctgcgca ttgtgctggt ctccacggcg cggcctcgga gccaagacga
       61 ggtatggtga gatcaacaga ggaagagaca attgacattg ggaaaccagc ccactattca
      121 cagttctcaa aaggagtggc catgctgtac cacaaagtgc caacaatata acaccaagcc
      181 attaactaat cagagaaaat ggaactgaat gtgaagccag catggagcag cgtcattcct
      241 tagcgggcaa ctgtgacgat ggtgagaagt cagaaagaga ggctcaggga ttcgagcatc
      301 ggacctgtat ggactctggg gatccttcct ttgggcaaaa tgatccccct accattttgc
      361 ccattactgc tcctaaaaca tatgattcac tcacatcaca ggatatgcca gagacactga
      421 ctgacacaca ggcccttcat gaagagcaat ttcatctttt ggaccaaaat gggcagccta
      481 ttcaatatga tcttcagtct ttgggaaatt ctactgcaca aatgacaggg atggagcaag
      541 tggccatacc tcaggagccc tttgcagatg aacatagccc tcaagatatc tctgaagaga
      601 aacccactgg tgtaagagca gatgttctcg aagacagcac cagcaattac ggccttcgct
      661 caccagcatc cctcgtgcta cccaaaaagg caggagccag acttgttgaa gaacctttgc
      721 tggctcctct ccagccactt tcttgtaaca cacccatgtg ggcctgccgt cttaggagtt
      781 gtgagaaaat tggagattcc taccgtggct actgtgtaag tgagactgaa ttagaaagtg
      841 tcctaacttt tcacaagcag caaacacaga ctgtctgggg tactcgccag tctccaagcc
      901 cagccaaacc tgccacacgc ttgatgtgga agtctcagta tgtcccatat gacggaatcc
      961 catttgtcaa tgcaggaagt cgagctgtgg taatggagtg tcagtatgga ccaaggagaa
     1021 aaggttttca gttaaaaaaa atcagtgagc aagaaagtag gtcttgtcat ctctataaag
     1081 ctacttgtcc agccaggata tacattaaaa aggtacagaa atttcctgaa tatagagtac
     1141 ctacagaccc ccaaattgac aggaagataa tcagactgga gcaggagaaa gccttcacta
     1201 tgctgaagaa gaacctgatg gatgctggtg gtgtacttcg atggtatgtg cagttaccta
     1261 cacagcaagc tcatcagtat catgaatcag agactcctgg tgtccctttg tcaccgtcac
     1321 cttttcctat gtctcctctt gaagaagagg aagctatagt cagagatgag aactgtgcgt
     1381 tgccctctcg tctccacccg caagtagcgc ataagattca ggaactagtg tcacagggag
     1441 taggacaagt gtatgcagta aggaagcagc taagaaaatt tgtggaaaga gaactgttca
     1501 aacctgatga gatacctgaa agacataatt tatcttattt tccaactgta aatgatataa
     1561 aaaatcatat ccatgaggta cagaaatcct tgaggactgg agatgtggta tataactcag
     1621 agattattcc agcaacgctt cagtggacga cagatagtgg gaacatcctc agagagactg
     1681 taacagttac atttgcagaa ggaaatttac taggagaacc gattcccagc aaaatgggaa
     1741 caagtcagac acagactgct gtgtctccag agccactttc ttcatttcca cccaaaatat
     1801 ttacacactt tcaggctttg aagttacagc caagactctc ctctcctgat ggctcacaag
     1861 ctttggtatc agtagatagc catgcatcct ctagtcctcc tggccttgtg gatactgtag
     1921 gaaatgctga agtggataac cactctgtac tgcttggtca gagtcaaaac cctgggacag
     1981 atacatgcct aacccaagac aacagcacct cctcctccac gggccacctc ccagaatcag
     2041 ttccgaatcc agttgcagag gatcagctgc tggaaggtga agatgttgag gatgcaggga
     2101 atccggaagg aagtgtaaat aggactctgt tgggagatgt acaaaccgtt ccaatacaga
     2161 tcatagacag ccgtccagtg cttgttgaag aaagtctgtc taagaaccaa gttaaacaag
     2221 aaaccaatga gccaacactg tctacagaag caaaaacttt cctggactgt aaaaaaatat
     2281 ctgctacata aatattttaa gtttttcaga atttacagct ctggcccctt ctgatttctt
     2341 agtaggtgga gattgtctga gatggtgtat caatcactgg accagaaatg tatgatgggc
     2401 agttttgact taaagctcca atatttgttg ccaattctat atttgtattt cccttaagaa
     2461 atattttact ttattttgta ttcctttatt attattatta ttatccagta aggcaaatat
     2521 tttaaaagat catctgaagt tatccacagt aatttagtgt gaatgcaaat agttatctcg
     2581 ccaagttttg gtttttacaa gacagaaaag accaacaata aaagcagtgt gtaagtgcat
     2641 gtctaggtgt gtcagaaaat ttaaagttgt aagaacagtt ttgattaact gacaggtgag
     2701 aaactattta tcatctgttt atttggaaag gtagagcaat agatgtgtat ggtagcacag
     2761 agccaccaaa cacccccccc ccccaatgct gcatttgcag tttatccttg gcttacatct
     2821 ttaactgtaa atgagtggta ctgaaggtca tctgtcagac atttcagtga cttggaaata
     2881 ttaaaacatt gaattaatca cacattgaaa taacacaaat gaatgtaaat gacgtgtatg
     2941 ttatatggga ctggaggtaa atccctacct aggaatagaa acatgaaaaa ttcagagatt
     3001 tggggaaatg ttagaatttc ctactattta tagatcattt gggggaactt ttgatggtag
     3061 aaaaattgtt gttgcttttt aaagattcaa ttttgactag aaaataattt aaatagtatt
     3121 tacatttttt tctgtactag agttctttct gaattgatag tattacgatt tatatatttc
     3181 aaggtaagta tatatagctt aaaatttagt tcatatttga aacttaaaaa tgtctgatcc
     3241 gatatttact cattaaataa gtatatttat atttatacct agtaccaaat atcaagtaat
     3301 tagtatgttc attataacct tgtttttatg gaagattgac taggtagaaa tttcaaatgt
     3361 aactgtcctg aaataagcaa gctgaatgta gtggtacaca cctgtaatcc cagcactgag
     3421 gaggccaagg actcagattg taaattcaat tagcctaggt tatagagcaa gaccttacta
     3481 agaaagaaag agcggatcac ctagaagatt ctttttctgg agattcattt gtgagttact
     3541 tacttttttt tttttttttt ttttttgtct tttcgagaca gggtttctct gtgtagccct
     3601 ggctgtcctg gaactcactc tgtagaccag gctggcctgg aactcagaaa tccgcctgct
     3661 tctgcctccc gagtgctgga attaaaggtg tgtgccacca ccgcctggct aacattttta
     3721 tttttaaaaa taagaagttc aagtggatat taaagtttaa tattcaaagc atgaacaata
     3781 taatttgata tttgtcctta aaactttaca tggattgact ttcctaaaat ctaatttccc
     3841 attattattc actttgcttt gggtacatga cctgtaccta aagataaagc tctgttattt
     3901 taaaagtagg cagaactcta aaattcatta tctttacatc tgtatcattg tcaacataac
     3961 gtttattata tcttattgaa agcaaagttc atatataatt atatgtatga ttattcgtag
     4021 gaactgcttt tcttcttttc ttttttcttt ctttctttct ttttttttta agaaaaaaac
     4081 taaagatatc tgttgccagt atttatagca gcgggctact aagcctgaat atattacatt
     4141 aagtttaata caattcgtag tcacttcaaa acccaggttc tcttagtctg tgtgtactta
     4201 agaggaatga aatcaaatta gtatatagtt taggactgta taggtaatgc tcttttgtta
     4261 tccatgttat agaaactgtt tttcaagaac agataggttt tcatcttcca ataatgatta
     4321 cggtttattg ctaaatatct tacattcctt ttttatttgg actgctaagg agaaatgctt
     4381 gttaagaaac aaactagtca atttccagtg cggagagaaa gagcagagga cccagcggtt
     4441 actgtgtaat caggaacaag tacagtgagg atgtgtttct gccacctcat tccgccaggt
     4501 ggcaggcacc tatagtccca gtgcatctct gtgagctcca ggggagcccg gcccacacag
     4561 tgagatcagg acagccagag ctacatagtg aggcccagga agaaagaaaa gagataacat
     4621 atttctgtca cttcattctg tttgaatggg tcttatgtta atatttttta ttatgagaac
     4681 agaaagttac atacattctt cccacaaaca aaaagaaaac agaaaactga atacttatat
     4741 ctctaattgt atatttttgc tttcttaggt agaaactgga actattaata tttttgagta
     4801 tttaaataag tagtttttaa aaaatggtat ctaaatgagt ttaaaaatgt atttgtcttc
     4861 taaactcatg tatagtcaca acatgtcagt gttggctaaa ggtgcttagt accagttttt
     4921 gaaaactatg tttacatatt taaatattta attctttaca aatgtacaag atcttaaaca
     4981 aaagtgtata aaaatttcag tatctgtatt tggcgttctc ttaacaactc cacagcaaaa
     5041 tttggcaata ctcttttgtt catgtattac aagatgtagt atctgtagtg gaaagctgga
     5101 catttaaaat aagcagttag aaaatcataa tcatagcctg gcttgtttct ctgcagtttt
     5161 catggtctag gcctccaggt aaacttagta tatacccagg aaattacaga taaacaacca
     5221 caggagactt gaccctcggt agtaattttc tgtgtaagca gccaatgtac ttagagagct
     5281 ttaacatctt atgcagagtg aatgtttcct aactgatagg aaacataagc ttggagatcc
     5341 ggtcctgcat tgaagttttt aattttaagc cccagcttgt gctttatctt gcttatatcc
     5401 tgtcaagcta tatacttaat catgtgggtt tgagatagtg tttgctttat actagatttg
     5461 taaaaagatt ttaaaatatg tatgtatata ttgtaacttg atgtacagtg ttcttattat
     5521 aatgtaattt ttattaaaaa attctgtttt tttcttaaaa aaaaaaaaaa aaaa
//
