LOCUS       NM_181315               3465 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus carbonic anhydrase 5b, mitochondrial (Car5b), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_181315 NM_019513
VERSION     NM_181315.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Aspatwar,A., Supuran,C.T., Waheed,A., Sly,W.S. and Parkkila,S.
  TITLE     Mitochondrial carbonic anhydrase VA and VB: properties and roles in
            health and disease
  JOURNAL   J Physiol 601 (2), 257-274 (2023)
   PUBMED   36464834
  REMARK    GeneRIF: Mitochondrial carbonic anhydrase VA and VB: properties and
            roles in health and disease.
            Review article
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Gan,P., Patterson,M., Watanabe,H., Wang,K., Edmonds,R.A.,
            Reinholdt,L.G. and Sucov,H.M.
  TITLE     Allelic variants between mouse substrains BALB/cJ and BALB/cByJ
            influence mononuclear cardiomyocyte composition and cardiomyocyte
            nuclear ploidy
  JOURNAL   Sci Rep 10 (1), 7605 (2020)
   PUBMED   32371981
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3465)
  AUTHORS   Shah,G.N., Rubbelke,T.S., Hendin,J., Nguyen,H., Waheed,A.,
            Shoemaker,J.D. and Sly,W.S.
  TITLE     Targeted mutagenesis of mitochondrial carbonic anhydrases VA and VB
            implicates both enzymes in ammonia detoxification and glucose
            metabolism
  JOURNAL   Proc Natl Acad Sci U S A 110 (18), 7423-7428 (2013)
   PUBMED   23589845
  REMARK    GeneRIF: both Car5A and Car5B contribute to both ammonia
            detoxification (ureagenesis) and regulation of fasting blood sugar
            (gluconeogenesis).
REFERENCE   4  (bases 1 to 3465)
  AUTHORS   Wu,L., Sagong,B., Choi,J.Y., Kim,U.K. and Bok,J.
  TITLE     A systematic survey of carbonic anhydrase mRNA expression during
            mammalian inner ear development
  JOURNAL   Dev Dyn 242 (3), 269-280 (2013)
   PUBMED   23233153
REFERENCE   5  (bases 1 to 3465)
  AUTHORS   Archambaud,C., Nahori,M.A., Soubigou,G., Becavin,C., Laval,L.,
            Lechat,P., Smokvina,T., Langella,P., Lecuit,M. and Cossart,P.
  TITLE     Impact of lactobacilli on orally acquired listeriosis
  JOURNAL   Proc Natl Acad Sci U S A 109 (41), 16684-16689 (2012)
   PUBMED   23012479
REFERENCE   6  (bases 1 to 3465)
  AUTHORS   Price,T.O., Eranki,V., Banks,W.A., Ercal,N. and Shah,G.N.
  TITLE     Topiramate treatment protects blood-brain barrier pericytes from
            hyperglycemia-induced oxidative damage in diabetic mice
  JOURNAL   Endocrinology 153 (1), 362-372 (2012)
   PUBMED   22109883
REFERENCE   7  (bases 1 to 3465)
  AUTHORS   Pan,P.W., Waheed,A., Sly,W.S. and Parkkila,S.
  TITLE     Carbonic anhydrases in the mouse harderian gland
  JOURNAL   J Mol Histol 41 (6), 411-417 (2010)
   PUBMED   20820888
  REMARK    GeneRIF: Car5b and Car13 showed the highest signals in the mouse
            harderian gland.
REFERENCE   8  (bases 1 to 3465)
  AUTHORS   Cankaya,M., Hernandez,A.M., Ciftci,M., Beydemir,S., Ozdemir,H.,
            Budak,H., Gulcin,I., Comakli,V., Emircupani,T., Ekinci,D., Kuzu,M.,
            Jiang,Q., Eichele,G. and Kufrevioglu,O.I.
  TITLE     An analysis of expression patterns of genes encoding proteins with
            catalytic activities
  JOURNAL   BMC Genomics 8, 232 (2007)
   PUBMED   17626619
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 3465)
  AUTHORS   Pan,P.W., Rodriguez,A. and Parkkila,S.
  TITLE     A systematic quantification of carbonic anhydrase transcripts in
            the mouse digestive system
  JOURNAL   BMC Mol Biol 8, 22 (2007)
   PUBMED   17362527
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 3465)
  AUTHORS   Shah,G.N., Hewett-Emmett,D., Grubb,J.H., Migas,M.C., Fleming,R.E.,
            Waheed,A. and Sly,W.S.
  TITLE     Mitochondrial carbonic anhydrase CA VB: differences in tissue
            distribution and pattern of evolution from those of CA VA suggest
            distinct physiological roles
  JOURNAL   Proc Natl Acad Sci U S A 97 (4), 1677-1682 (2000)
   PUBMED   10677517
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY121187.1, AK085670.1, BX469932.7 and CD802657.1.
            
            On or before Nov 1, 2008 this sequence version replaced
            NM_019513.1, NM_181315.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034413.1, AF192978.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                BY121187.1         2-27
            27-931              AK085670.1         2-906
            932-2893            BX469932.7         83540-85501         c
            2894-3465           CD802657.1         1-572               c
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 76.03 cM"
     gene            1..3465
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /note="carbonic anhydrase 5b, mitochondrial"
                     /db_xref="GeneID:56078"
                     /db_xref="MGI:MGI:1926249"
     exon            1..76
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            77..268
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..1080
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /EC_number="4.2.1.1"
                     /note="carbonic anhydrase VB; CA-VB; carbonate dehydratase
                     VB"
                     /codon_start=1
                     /product="carbonic anhydrase 5B, mitochondrial precursor"
                     /protein_id="NP_851832.2"
                     /db_xref="CCDS:CCDS30515.1"
                     /db_xref="GeneID:56078"
                     /db_xref="MGI:MGI:1926249"
                     /translation="MAVMNHLRVILQVSSSTLPWRRCWVPRLVPRRSCSLYTCTYRTR
                     NRALPPLWENLDLVPAGDRQSPINIRWRDSVYDPGLKPLTISYDPATCLHIWNNGYSF
                     LVEFEDSTDKSVVEGGPLEHNYRLKQFHFHWGAIDAWGSEHTVDSKCYPAELHLVHWN
                     AVKFESFEDAALEENGLAVIGVFLKLGKHHKELQKLVDTLPSIKHKDTLVEFGSFDPS
                     CLMPTCPDYWTYSGSLTTPPLSESVTWIIKKQPVEVDRDQLEQFRTLLFTSEGEKEKR
                     MVDNFRPLQPLMNRTVRSSFRHDYVLNIQVKPKPTASEVTP"
     exon            269..466
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            467..585
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            586..681
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            682..744
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            745..900
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            901..3449
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2450
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /note="major polyA site"
     polyA_site      2481
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
     regulatory      2598..2603
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /note="hexamer: ATTATA"
     polyA_site      2618
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
     regulatory      3408..3413
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3432
                     /gene="Car5b"
                     /gene_synonym="7330410H16Rik; Ca5b; CarVb; CAVB;
                     D730005F19Rik"
ORIGIN      
        1 agtagctggt cctgggaagc taacttgcca gtggcacttg ttaagtgctg ttggagaaac
       61 ggagaaaaac gtccaggtct taggttcccg agctgcttag ccattgctgg gtattccaag
      121 ctaaagatgg ctgtgatgaa tcacctgaga gtgattctac aagtctcttc gagtacattg
      181 ccatggagaa ggtgctgggt tccgaggttg gtgccacgga ggtcctgcag tctctatacc
      241 tgcacataca gaaccaggaa ccgagcctta cctccactct gggagaactt ggaccttgtt
      301 cctgcgggtg atcgacaatc acccatcaac atccgatgga gggacagtgt ttatgaccct
      361 ggcttaaaac cactcaccat ttcttatgac ccagccacct gcctccacat ctggaacaat
      421 gggtactctt ttcttgtgga atttgaagat tctacagata aatcagtggt cgaggggggg
      481 cccctggaac acaactaccg actgaagcag tttcattttc attggggagc cattgatgct
      541 tggggctctg agcacaccgt ggacagtaaa tgctacccag cagagctgca cttggtacat
      601 tggaacgcag tcaaatttga aagttttgag gatgcagcac tggaagaaaa tggcttggct
      661 gtgataggtg tatttttaaa gcttggtaaa catcataaag aactacagaa attggtggac
      721 actttgccat caattaagca caaggacacc ctggtggaat ttggatcatt tgacccttct
      781 tgtctgatgc ctacttgccc agattactgg acctactctg ggtctctcac tacgccgccc
      841 ctctcagagt ctgtcacctg gatcattaag aagcagccag ttgaggttga ccgtgaccag
      901 cttgagcagt ttcggaccct gcttttcact tctgagggag agaaagagaa aagaatggtg
      961 gacaacttcc gccctcttca gccgctgatg aatcgcacag tccgctcatc cttccgccat
     1021 gactatgtgc tcaatataca agtgaagccc aagccaacag ccagcgaagt caccccctaa
     1081 gacagcattc ttcagcagca tcttgtttta atgactatta gcttaaaatt ttaactagat
     1141 tattttaaaa cacagcattt aataatactt atatgtatac atttctattt ttttcttcct
     1201 tttttgggta acatttcaaa attcctactc ttgtaaagca aagaccggga gacaaacgaa
     1261 gaaaaacaaa aacctgtacc caaataacgt caatctgagg tcaccctttc tttctttctt
     1321 tctttctttc tttctttctt tctttctttc tttctttctt tcttctttct ttctttcttt
     1381 ctttctttct ttcttttttt tttttgagac agggtttctc tgtgtagtct tggctagcct
     1441 ggaactcact ttgtagacca ggctggcctc gaactcagaa atccacctgt ctctgcctcc
     1501 caagtgctgg gattaaaggc gtgcgccacc acgctcagca gatttactta ttattatatg
     1561 taagtatgat gtagctgtct ttagacacac cagaagaggg tgtcaggtct cattatggat
     1621 ggttgtgagc cacggttgct gggaattgaa ctcaggactt tcagaagagc agtcagtgct
     1681 cttaactgct gagccatctc tccagtccag aggtcaccct ttcttatgac agcgttaagg
     1741 cagaggaatc tgccaggcag tggtggcgca cggctttaat cccagcactt gggaggcaga
     1801 gacaggtgga tttctgagtt cgaggccagc ctggtctaca aagtgagttc caggacagcc
     1861 agggctacac agagaaaccc tatctcgggg aaaaagaaaa aaaaaaggca gaggaatctt
     1921 cattaactgt taaatctctc taatctattt ctcagacagt cagaaagaca atttagtttc
     1981 cttttggtta aatgggggct taatatgagt gcctgatctt gatttttctg tttgggtcta
     2041 attcaggagc ttagctcaca acaagaggtt cttgtgaatt tgattttaaa atgcccccct
     2101 tttgttcaaa ctggtgaatt gtctcacctt tcattgttgg tcaagttctt agctaccatc
     2161 atttggaggt gaatggctct caaagtttag gtgtaaactt cctggtttta gagtatttca
     2221 tgtctttttt aaaagagaag gtctcatctg tgtagccctg gctgtcctgg aaatcactat
     2281 gtaaaccagt ctagacttga actcacagaa gtctgtctgt ctttgcctcc caagtgctgg
     2341 gaataaaggc ttgtgccacc aaggccagct ttcattatat ttgctctttg actgataata
     2401 atatgtgtca ttggaatatt aacagaacat gcaagttgca cttatagcaa tctgttagta
     2461 attgtgtttt tatatctgaa attgtgtatg tgtgtgtgtt ttgagacagt gtcccactct
     2521 gtagtccagg ctggcctgga actcatagtt aaacttttgt ctcagtctct caagtgtaca
     2581 aggcaccaca ctcaacgatt atatttgcct tccttggcta tcctttttat ttgtgaaaca
     2641 aggtatcctt tagctcaggc tgtattagaa ggtgctgtgt agcaggagct gctttgaagc
     2701 ttcaggtcct cctgcttcca catcccaact gctagcattg caagtgtgcg ccactccgct
     2761 cagcttcagc tctcttccag tgttcatagc tgtgtagaat cacaagtagg gattacagtc
     2821 aaactgttaa aaactaccaa tggtggtgct acttaccaca caaagcccag gcttctcaga
     2881 aaacgtctgt gggagcccag accagcaagt ccgagctgaa tgctggccat agttttaggt
     2941 atattttgtt tcaaaacttg ggtgtgaatt tttgtattca tacttctcaa tgagtatgtt
     3001 tatactaatc tttagttgct attactagca tatgatatgt ctacttatta tcaatttact
     3061 gtatagaaaa tacatttggt agtcatgggc tggctcagtg ggtaaagaca cttgccatgt
     3121 gaacctaaag acctgagctt gatccccaga gctcacataa ctaaccccac caaattgctg
     3181 tctgaccaag catctttcat cccatacata caaccacata cacattataa atgcacacaa
     3241 taataaataa aactttgggg gacggttcaa gacagggttt ctctggatag ccctggctgt
     3301 tctggaaact cactctgtag accaggctgg cctagaactc agaaatccac ctgcctctgc
     3361 ctcccaagtg ctgacattaa aggtatgcgc cgccactgcc tgatctaaat aaaactttaa
     3421 aagaataaaa aagaagctat attttgtaaa aaaaaaaaaa aaaaa
//
