LOCUS       NM_001037761            1789 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus capping actin protein of muscle Z-line subunit beta
            (Capzb), transcript variant 1, mRNA.
ACCESSION   NM_001037761
VERSION     NM_001037761.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Ye,Z., Wang,D., Lu,Y., He,Y., Yu,J., Wei,W., Chen,C., Wang,R.,
            Zhang,L., Zhang,L., Le,M.T.N., Cho,W.C., Yang,M., Zhang,H. and
            Yue,J.
  TITLE     Vacuolin-1 inhibits endosomal trafficking and metastasis via
            CapZbeta
  JOURNAL   Oncogene 40 (10), 1775-1791 (2021)
   PUBMED   33564074
  REMARK    GeneRIF: Vacuolin-1 inhibits endosomal trafficking and metastasis
            via CapZbeta.
REFERENCE   3  (bases 1 to 1789)
  AUTHORS   Jo,Y.J., Jang,W.I., Namgoong,S. and Kim,N.H.
  TITLE     Actin-capping proteins play essential roles in the asymmetric
            division of maturing mouse oocytes
  JOURNAL   J Cell Sci 128 (1), 160-170 (2015)
   PUBMED   25395583
REFERENCE   4  (bases 1 to 1789)
  AUTHORS   Kim,T., Cooper,J.A. and Sept,D.
  TITLE     The interaction of capping protein with the barbed end of the actin
            filament
  JOURNAL   J Mol Biol 404 (5), 794-802 (2010)
   PUBMED   20969875
  REMARK    GeneRIF: Capping protein interacts with both actin protomers at the
            barbed end of the filament, and the amphipathic helix at the
            C-terminus of the beta-subunit binds to the hydrophobic cleft on
            actin.
REFERENCE   5  (bases 1 to 1789)
  AUTHORS   Davis,D.A., Wilson,M.H., Giraud,J., Xie,Z., Tseng,H.C., England,C.,
            Herscovitz,H., Tsai,L.H. and Delalle,I.
  TITLE     Capzb2 interacts with beta-tubulin to regulate growth cone
            morphology and neurite outgrowth
  JOURNAL   PLoS Biol 7 (10), e1000208 (2009)
   PUBMED   19806181
  REMARK    GeneRIF: Capzb2 plays an important role in growth cone formation
            and neurite outgrowth and that the underlying mechanism may involve
            direct interaction between Capzb2 and microtubules.
REFERENCE   6  (bases 1 to 1789)
  AUTHORS   Geyer,C.B., Inselman,A.L., Sunman,J.A., Bornstein,S., Handel,M.A.
            and Eddy,E.M.
  TITLE     A missense mutation in the Capza3 gene and disruption of F-actin
            organization in spermatids of repro32 infertile male mice
  JOURNAL   Dev Biol 330 (1), 142-152 (2009)
   PUBMED   19341723
REFERENCE   7  (bases 1 to 1789)
  AUTHORS   Hart,M.C. and Cooper,J.A.
  TITLE     Vertebrate isoforms of actin capping protein beta have distinct
            functions In vivo
  JOURNAL   J Cell Biol 147 (6), 1287-1298 (1999)
   PUBMED   10601341
REFERENCE   8  (bases 1 to 1789)
  AUTHORS   von Bulow,M., Rackwitz,H.R., Zimbelmann,R. and Franke,W.W.
  TITLE     CP beta3, a novel isoform of an actin-binding protein, is a
            component of the cytoskeletal calyx of the mammalian sperm head
  JOURNAL   Exp Cell Res 233 (1), 216-224 (1997)
   PUBMED   9184090
REFERENCE   9  (bases 1 to 1789)
  AUTHORS   Marks,P.W. and Kwiatkowski,D.J.
  TITLE     Genomic organization and chromosomal location of murine Cdc42
  JOURNAL   Genomics 38 (1), 13-18 (1996)
   PUBMED   8954774
REFERENCE   10 (bases 1 to 1789)
  AUTHORS   Schafer,D.A., Korshunova,Y.O., Schroer,T.A. and Cooper,J.A.
  TITLE     Differential localization and sequence analysis of capping protein
            beta-subunit isoforms of vertebrates
  JOURNAL   J Cell Biol 127 (2), 453-465 (1994)
   PUBMED   7929588
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY033332.1, BY124858.1,
            U10406.1, BQ030615.1 and BY415700.1.
            
            On Sep 27, 2012 this sequence version replaced NM_001037761.1.
            
            Summary: This gene encodes the beta subunit of a highly conserved
            filamentous actin capping protein that binds the barbed end of
            filamentous actin to stabilize it and terminate elongation.
            Interaction of this protein with the barbed end of the actin
            filament occurs through binding of the amphipathic helix at the
            C-terminus to the hydrophobic cleft on the actin molecule. This
            gene is required for a variety of dynamic actin-mediated processes
            including organization of lamellipodia and filopodia, growth cone
            morphology and neurite outgrowth in hippocampal neurons, and
            asymmetric spindle migration and polar body extrusion during oocyte
            maturation. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U10406.1, AK159390.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-74                BY033332.1         1-74
            75-80               BY124858.1         64-69
            81-1727             U10406.1           1-1647
            1728-1756           BQ030615.1         47-75               c
            1757-1789           BY415700.1         271-303
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 70.59 cM"
     gene            1..1789
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /note="capping actin protein of muscle Z-line subunit
                     beta"
                     /db_xref="GeneID:12345"
                     /db_xref="MGI:MGI:104652"
     exon            1..130
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..961
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /note="isoform a is encoded by transcript variant 1;
                     F-actin-capping protein subunit beta; capping protein
                     (actin filament) muscle Z-line, beta; capZ beta; capping
                     protein beta 1"
                     /codon_start=1
                     /product="F-actin-capping protein subunit beta isoform a"
                     /protein_id="NP_001032850.1"
                     /db_xref="CCDS:CCDS18842.1"
                     /db_xref="GeneID:12345"
                     /db_xref="MGI:MGI:104652"
                     /translation="MSDQQLDCALDLMRRLPPQQIEKNLSDLIDLVPSLCEDLLSSVD
                     QPLKIARDKVVGKDYLLCDYNRDGDSYRSPWSNKYDPPLEDGAMPSARLRKLEVEANN
                     AFDQYRDLYFEGGVSSVYLWDLDHGFAGVILIKKAGDGSKKIKGCWDSIHVVEVQEKS
                     SGRTAHYKLTSTVMLWLQTNKSGSGTMNLGGSLTRQMEKDETVSDCSPHIANIGRLVE
                     DMENKIRSTLNEIYFGKTKDIVNGLRSLDAIPDNHKFKQLQRELSQVLTQRQVYIQPD
                     N"
     exon            131..220
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /inference="alignment:Splign:2.1.0"
     exon            221..342
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /inference="alignment:Splign:2.1.0"
     exon            343..456
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /inference="alignment:Splign:2.1.0"
     exon            457..598
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /inference="alignment:Splign:2.1.0"
     exon            599..715
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /inference="alignment:Splign:2.1.0"
     exon            716..781
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /inference="alignment:Splign:2.1.0"
     exon            782..858
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /inference="alignment:Splign:2.1.0"
     exon            859..971
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1789
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1213..1218
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /note="hexamer: AATAAA"
     polyA_site      1235
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /note="major polyA site"
     regulatory      1761..1766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /note="hexamer: AATAAA"
     regulatory      1771..1776
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
                     /note="hexamer: AAGAAA"
     polyA_site      1782
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
     polyA_site      1787
                     /gene="Capzb"
                     /gene_synonym="1700120C01Rik; Cappb1; CPB1; CPB2; CPbeat2;
                     CPbeta1; CPbeta2"
ORIGIN      
        1 ggggggggag gggggcgctg acccggatgt tcactcttgg gcacccgggg aagtggaagc
       61 gcagggccct gctgcggggg ggagagccac agacgccggg acagggaccg tcgccgccgc
      121 cgccaccatg agcgatcagc agctggactg cgccttggac ctgatgaggc gcctgcctcc
      181 acagcagatt gagaagaacc tcagcgatct gatcgacctg gtccccagtc tgtgtgaaga
      241 tctcctgtca tctgttgacc agcccctgaa aattgccaga gacaaggtgg tgggcaagga
      301 ttaccttttg tgtgactaca acagagacgg ggactcctat aggtcaccgt ggagtaacaa
      361 gtatgaccct cctttggaag atggggccat gccatctgct cggctcagaa agctggaggt
      421 agaggccaac aatgccttcg accaataccg agacctgtat tttgaaggtg gggtctcatc
      481 agtctacctc tgggatcttg atcatggctt tgctggagtg atcctcataa agaaagctgg
      541 agatggatcc aagaagatca aaggctgctg ggattccatc cacgtggtgg aagtgcagga
      601 gaagtccagc ggccgtactg cccattacaa gttgacctcc acggtgatgc tatggctgca
      661 aaccaacaaa tccggctcgg gcaccatgaa cctgggaggc agcctaacca gacagatgga
      721 gaaagacgaa actgtgagtg actgttcccc acacatagcc aacatcgggc gcctggtgga
      781 ggacatggaa aacaaaatcc gaagcacgct gaatgagatc tactttggaa aaacaaagga
      841 catcgtcaac gggctgagat ctcttgatgc tatccccgac aaccacaagt ttaagcagtt
      901 gcagagggaa ctttctcaag tgctgaccca gcgccaggtc tacatccagc ctgataattg
      961 agccgaccca ggtctgtgca gacgtttgca gacaaatcaa agcaagaagc gcttaagaac
     1021 gacctggtgg aggccttgaa gagaaagcag cagtgttgaa gacctctgct tcacgctaac
     1081 cggacacgcc atgcactcgt taggttcctt tctttagaaa actcgttttc tgctcctttt
     1141 ccctcttcct ttcccgccct gacaggtcac ataacagttt gcatcgacca cgcagcgcca
     1201 tctctccccc aaaataaagt ccgataacca ccctcctctg gctccaaggc ctgcttccca
     1261 cgacgtttcc atagagaccg tgtggttttg ttcgcctgtc ccccttcctt cccttgccca
     1321 tttataggca cagatacact gtctgacacc gctccctcca tctttttgtt acattggtgt
     1381 aaaaaatgta aaacaaaaaa ttttatgaac taactggtgt gtgagagaag aaactggaga
     1441 tctgatccgt gtgtgtgtgg gagttgcttg gagtcagggc tggggacagg ggacagcagt
     1501 ggaggaagga gtgatgtcct cagcactgtc ctgcacaggt ggacccttgt cccaaggaga
     1561 ccatgctggg gtggggtggg gctggcgacc ggctgctctt caggccaggt gcttttctgt
     1621 caatttttat ggaatgcaaa aggagttttt tgttttattt tggttttttt ttgtaaagct
     1681 taaacagaca aaaaaatcta catcttctac ttgagcctcc atacttaaaa aaaaaaaaaa
     1741 aggaaaaaaa agaacaaaaa aataaataaa aagaaactgg gacacaatc
//
