LOCUS       NM_009795               1559 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus calpain, small subunit 1 (Capns1), mRNA.
ACCESSION   NM_009795 XM_001000249 XM_001000263 XM_001000279 XM_001000296
            XM_001000311
VERSION     NM_009795.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Wang,Y., Li,M., Chen,J., Yu,Y., Yu,Y., Shi,H., Liu,X., Chen,Z.,
            Chen,R. and Ge,J.
  TITLE     Macrophage CAPN4 regulates CVB3-induced cardiac inflammation and
            injury by promoting NLRP3 inflammasome activation and phenotypic
            transformation to the inflammatory subtype
  JOURNAL   Free Radic Biol Med 208, 430-444 (2023)
   PUBMED   37660839
  REMARK    GeneRIF: Macrophage CAPN4 regulates CVB3-induced cardiac
            inflammation and injury by promoting NLRP3 inflammasome activation
            and phenotypic transformation to the inflammatory subtype.
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Zhang,L., Zheng,D., Yan,Y., Yu,Y., Chen,R., Li,Z., Greer,P.A.,
            Peng,T. and Wang,Q.
  TITLE     Myeloid cell-specific deletion of Capns1 prevents macrophage
            polarization toward the M1 phenotype and reduces interstitial lung
            disease in the bleomycin model of systemic sclerosis
  JOURNAL   Arthritis Res Ther 24 (1), 148 (2022)
   PUBMED   35729674
  REMARK    GeneRIF: Myeloid cell-specific deletion of Capns1 prevents
            macrophage polarization toward the M1 phenotype and reduces
            interstitial lung disease in the bleomycin model of systemic
            sclerosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1559)
  AUTHORS   Akasu,R., Miyazaki,T., Elhussiny,M.Z., Sugiura,Y., Tomitsuka,Y.,
            Haraguchi,S., Otsu,K., Chowdhury,V.S. and Miyazaki,A.
  TITLE     Calpain-mediated proteolytic production of free amino acids in
            vascular endothelial cells augments obesity-induced hepatic
            steatosis
  JOURNAL   J Biol Chem 298 (6), 101953 (2022)
   PUBMED   35447117
REFERENCE   4  (bases 1 to 1559)
  AUTHORS   Huang,X., Zhang,K.J., Jiang,J.J., Jiang,S.Y., Lin,J.B. and Lou,Y.J.
  TITLE     Identification of Crucial Genes and Key Functions in Type 2
            Diabetic Hearts by Bioinformatic Analysis
  JOURNAL   Front Endocrinol (Lausanne) 13, 801260 (2022)
   PUBMED   35242109
  REMARK    GeneRIF: Identification of Crucial Genes and Key Functions in Type
            2 Diabetic Hearts by Bioinformatic Analysis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1559)
  AUTHORS   Li,L., Thompson,J., Hu,Y., Lesnefsky,E.J., Willard,B. and Chen,Q.
  TITLE     Calpain-mediated protein targets in cardiac mitochondria following
            ischemia-reperfusion
  JOURNAL   Sci Rep 12 (1), 138 (2022)
   PUBMED   34997008
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1559)
  AUTHORS   Li,J., Nixon,R., Messer,A., Berman,S. and Bursztajn,S.
  TITLE     Altered gene expression for calpain/calpastatin system in motor
            neuron degeneration (Mnd) mutant mouse brain and spinal cord
  JOURNAL   Brain Res Mol Brain Res 53 (1-2), 174-186 (1998)
   PUBMED   9473662
REFERENCE   7  (bases 1 to 1559)
  AUTHORS   Sorimachi,H., Ishiura,S. and Suzuki,K.
  TITLE     Structure and physiological function of calpains
  JOURNAL   Biochem J 328 (Pt 3) (Pt 3), 721-732 (1997)
   PUBMED   9396712
  REMARK    Review article
REFERENCE   8  (bases 1 to 1559)
  AUTHORS   Spencer,M.J. and Tidball,J.G.
  TITLE     Calpain translocation during muscle fiber necrosis and regeneration
            in dystrophin-deficient mice
  JOURNAL   Exp Cell Res 226 (2), 264-272 (1996)
   PUBMED   8806430
REFERENCE   9  (bases 1 to 1559)
  AUTHORS   Li,J., Grynspan,F., Berman,S., Nixon,R. and Bursztajn,S.
  TITLE     Regional differences in gene expression for calcium activated
            neutral proteases (calpains) and their endogenous inhibitor
            calpastatin in mouse brain and spinal cord
  JOURNAL   J Neurobiol 30 (2), 177-191 (1996)
   PUBMED   8738748
REFERENCE   10 (bases 1 to 1559)
  AUTHORS   Ohno,S., Minoshima,S., Kudoh,J., Fukuyama,R., Shimizu,Y.,
            Ohmi-Imajoh,S., Shimizu,N. and Suzuki,K.
  TITLE     Four genes for the calpain family locate on four distinct human
            chromosomes
  JOURNAL   Cytogenet Cell Genet 53 (4), 225-229 (1990)
   PUBMED   2209092
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY133208.1, BY296628.1, BC090988.1 and BU700497.1.
            
            On Jan 19, 2017 this sequence version replaced NM_009795.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC090988.1, AF058298.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                BY133208.1         2-23
            23-105              BY296628.1         1-83
            106-1535            BC090988.1         1-1430
            1536-1559           BU700497.1         1-24                c
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 17.31 cM"
     gene            1..1559
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /note="calpain, small subunit 1"
                     /db_xref="GeneID:12336"
                     /db_xref="MGI:MGI:88266"
     exon            1..148
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /inference="alignment:Splign:2.1.0"
     exon            149..372
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /inference="alignment:Splign:2.1.0"
     CDS             164..970
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /EC_number="3.4.22.17"
                     /note="calpain 4; CANP small subunit; calpain regulatory
                     subunit; calcium-dependent protease small subunit 1;
                     calcium-activated neutral proteinase small subunit"
                     /codon_start=1
                     /product="calpain small subunit 1"
                     /protein_id="NP_033925.2"
                     /db_xref="CCDS:CCDS21082.1"
                     /db_xref="GeneID:12336"
                     /db_xref="MGI:MGI:88266"
                     /translation="MFLVNSFLKGGGGGGGGGGLGGGLGNVLGGLISGAAGGGGGGGG
                     GMGLGGGGGGGGTAMRILGGVISAISEAAAQYNPEPPPPRSHYSNIEANESEEVRQFR
                     KLFVQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDSDTTGKLG
                     FEEFKYLWNNIKKWQAIYKRFDTDRSGTIGSHELPGAFEAAGFHLNEHLYSMIIRRYA
                     DESGNMDFDNFISCLVRLDAMFRAFKSLDKNGTGQIQVNIQEWLQLTMYS"
     exon            373..406
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /inference="alignment:Splign:2.1.0"
     exon            407..496
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /inference="alignment:Splign:2.1.0"
     exon            497..554
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /inference="alignment:Splign:2.1.0"
     exon            555..619
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /inference="alignment:Splign:2.1.0"
     exon            620..688
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /inference="alignment:Splign:2.1.0"
     exon            689..767
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /inference="alignment:Splign:2.1.0"
     exon            768..884
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /inference="alignment:Splign:2.1.0"
     exon            885..943
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1543
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1462..1467
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /note="hexamer: AATAAA"
     polyA_site      1487
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /note="major polyA site"
     polyA_site      1535
                     /gene="Capns1"
                     /gene_synonym="Capa-4; Capa4; Capn4; Cdps; Css1;
                     D7Ertd146e"
                     /note="major polyA site"
ORIGIN      
        1 aatggaggct ctaggaccgc gcagacgacg gctgggtggc ggcagggtct aacccggagt
       61 agctgcctga gtcaccggcc ccgccctccc gaaccggaca cagcggaggc cctgcagcag
      121 gcgagcaaga ccgcggaccg ccggatccga ctcagctgcg atcatgttct tggtgaattc
      181 gttcttgaag ggcggcggtg gcggtggggg aggcgggggt ctgggcgggg gcctggggaa
      241 tgtgctcgga ggcctgatca gcggagctgc tggcggcggc ggcggtggcg gtggaggcat
      301 gggactggga ggaggtggcg gaggcggtgg aactgccatg cgcatcctgg gcggagtcat
      361 tagcgccatc agcgaggctg ctgcgcagta caacccggag cctccgcctc cacgtagtca
      421 ttactccaat attgaggcca acgagagtga agaagtccgt cagttcagga aactttttgt
      481 ccagctggct ggagacgaca tggaggtcag cgccacagaa ctcatgaata tcctcaacaa
      541 ggtcgtgacc cgacacccgg atctgaaaac tgatggtttt ggcattgaca cttgtcggag
      601 catggtggcc gtcatggata gcgacaccac aggcaagctg ggctttgagg agttcaaata
      661 cctctggaac aacattaaaa agtggcaggc tatatacaaa cgctttgata ctgaccgatc
      721 agggactatc ggtagccatg aacttccagg ggcctttgag gcagcaggat tccacctgaa
      781 tgagcatctc tacagcatga tcatccggcg ctacgcagat gagagcggga acatggattt
      841 cgataacttc atcagctgct tggtcaggct agatgccatg ttccgcgctt tcaaatctct
      901 tgacaaaaat ggcaccggac agatccaagt gaacatccag gagtggctgc agctgactat
      961 gtattcctga atgggagtgg cagacctgcc ccctcacagc tcactatgga gccaccttga
     1021 accctgatcc tgactgcagc cgcattttca tggtcatgtc ctgctaccca gaggcctttc
     1081 gtctccctgt tctggtaccc agcttcccaa tcagcagcca gagctcaaca tgccacacct
     1141 tgctgcaccc tgagttaccc ctggctctga gacaagctag cttgttctac tgaagggtca
     1201 ccccacccta gcacacccaa tccacgccat ctgttacctt catcctgtct gtgtgccaag
     1261 cctagcactc ctgctgcttc catgtcccaa gggccctgtc ttagttctgg gaggatcaca
     1321 cctgtcccta cacaccctgt tacacactca cacagttacc tcccaggcag ccagatgaac
     1381 tccaggccat ggcaggtcca cacaccctag tgcctacgcc tgctctgtgc tccccctgca
     1441 cagcggtcgg gcccaggaga aaataaactc tctctacttg ctgcttcctt cggactctga
     1501 gtgctgagtc caggcaggag ggaacatgag ctctatagag taaaaaaaaa aaaaaaaaa
//
