LOCUS       NM_130890               2411 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus calpain 8 (Capn8), transcript variant 1, mRNA.
ACCESSION   NM_130890
VERSION     NM_130890.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Holmes,G., Gonzalez-Reiche,A.S., Lu,N., Zhou,X., Rivera,J.,
            Kriti,D., Sebra,R., Williams,A.A., Donovan,M.J., Potter,S.S.,
            Pinto,D., Zhang,B., van Bakel,H. and Jabs,E.W.
  TITLE     Integrated Transcriptome and Network Analysis Reveals
            Spatiotemporal Dynamics of Calvarial Suturogenesis
  JOURNAL   Cell Rep 32 (1), 107871 (2020)
   PUBMED   32640236
REFERENCE   3  (bases 1 to 2411)
  AUTHORS   Hata,S., Kitamura,F., Yamaguchi,M., Shitara,H., Murakami,M. and
            Sorimachi,H.
  TITLE     A Gastrointestinal Calpain Complex, G-calpain, Is a Heterodimer of
            CAPN8 and CAPN9 Calpain Isoforms, Which Play Catalytic and
            Regulatory Roles, Respectively
  JOURNAL   J Biol Chem 291 (53), 27313-27322 (2016)
   PUBMED   27881674
REFERENCE   4  (bases 1 to 2411)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 2411)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 2411)
  AUTHORS   Hata,S., Abe,M., Suzuki,H., Kitamura,F., Toyama-Sorimachi,N.,
            Abe,K., Sakimura,K. and Sorimachi,H.
  TITLE     Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease
            complex, G-calpain, involved in gastric mucosal defense
  JOURNAL   PLoS Genet 6 (7), e1001040 (2010)
   PUBMED   20686710
  REMARK    GeneRIF: gastrointestinal-tract-specific calpains 8 and 9 formed a
            complex we termed 'G-calpain,' in which both were essential for
            gastric mucosal defense
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2411)
  AUTHORS   Harrill,A.H., Watkins,P.B., Su,S., Ross,P.K., Harbourt,D.E.,
            Stylianou,I.M., Boorman,G.A., Russo,M.W., Sackler,R.S.,
            Harris,S.C., Smith,P.C., Tennant,R., Bogue,M., Paigen,K.,
            Harris,C., Contractor,T., Wiltshire,T., Rusyn,I. and
            Threadgill,D.W.
  TITLE     Mouse population-guided resequencing reveals that variants in CD44
            contribute to acetaminophen-induced liver injury in humans
  JOURNAL   Genome Res 19 (9), 1507-1515 (2009)
   PUBMED   19416960
REFERENCE   8  (bases 1 to 2411)
  AUTHORS   Hata,S., Doi,N., Kitamura,F. and Sorimachi,H.
  TITLE     Stomach-specific calpain, nCL-2/calpain 8, is active without
            calpain regulatory subunit and oligomerizes through C2-like domains
  JOURNAL   J Biol Chem 282 (38), 27847-27856 (2007)
   PUBMED   17646163
  REMARK    GeneRIF: nCL-2/calpain 8 is active without calpain regulatory
            subunit and oligomerizes through C2-like domains
REFERENCE   9  (bases 1 to 2411)
  AUTHORS   Hata,S., Koyama,S., Kawahara,H., Doi,N., Maeda,T.,
            Toyama-Sorimachi,N., Abe,K., Suzuki,K. and Sorimachi,H.
  TITLE     Stomach-specific calpain, nCL-2, localizes in mucus cells and
            proteolyzes the beta-subunit of coatomer complex, beta-COP
  JOURNAL   J Biol Chem 281 (16), 11214-11224 (2006)
   PUBMED   16476741
  REMARK    GeneRIF: nCL-2, localizes in mucus cells and proteolyzes the
            beta-subunit of coatomer complex, beta-COP
REFERENCE   10 (bases 1 to 2411)
  AUTHORS   Hata,S., Nishi,K., Kawamoto,T., Lee,H.J., Kawahara,H., Maeda,T.,
            Shintani,Y., Sorimachi,H. and Suzuki,K.
  TITLE     Both the conserved and the unique gene structure of
            stomach-specific calpains reveal processes of calpain gene
            evolution
  JOURNAL   J Mol Evol 53 (3), 191-203 (2001)
   PUBMED   11523006
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK132449.1, BC125504.1, AB050202.1 and AC123793.5.
            
            On Mar 11, 2009 this sequence version replaced NM_130890.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB050202.1, BC125508.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-324               AK132449.1         2-325
            325-2299            BC125504.1         297-2271
            2300-2386           AB050202.1         2296-2382
            2387-2387           AC123793.5         158048-158048
            2388-2411           AB050202.1         2383-2406
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 85.37 cM"
     gene            1..2411
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /note="calpain 8"
                     /db_xref="GeneID:170725"
                     /db_xref="MGI:MGI:2181366"
     exon            1..291
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..2166
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /EC_number="3.4.22.53"
                     /note="isoform 1 is encoded by transcript variant 1; new
                     calpain 2; stomach-specific M-type calpain;
                     stomach-specific calpain (nCL-2)"
                     /codon_start=1
                     /product="calpain-8 isoform 1"
                     /protein_id="NP_570960.2"
                     /db_xref="CCDS:CCDS35814.1"
                     /db_xref="GeneID:170725"
                     /db_xref="MGI:MGI:2181366"
                     /translation="MAALAAGISKQRAAAQGLGSNQNAVKYLGQDFETLRKQCLNSGV
                     LFKDPEFPACPSALGYRDLGPGSAETQGIIWKRPTELCSNPQFIVGGATRTDIRQGGL
                     GDCWLLAAIASLTLNEKLLYRVVPRDQSFQKNYAGIFHFQFWQYGEWVEVVIDDRLPT
                     KNGQLLFLHSEEGNEFWSALLEKAYAKLNGSYEALAGGSTIEGFEDFTGGISEFYDLR
                     KPPGNLYYTIQKALRKGSLLGCSIDVSNAAEAEATTRQKLVKGHAYSVTGVEEVDFRG
                     LPEKLIRLRNPWGEVEWTGAWSDSAPEWNYIDPQKKGELDKRAEDGEFWMSFSDFLKQ
                     FSRLEICNLSPDSLSSEEIHKWNLVLFNGRWTRGSTAGGCQNYPATYWTNPQFKIHLD
                     EVDEDQEEGTSEPCCTVLLGLMQKNRRRQRRIGQGMLSIGYAVYQIPKELENHTDEHL
                     GRDFFQGRQPSTCSSTYMNLREVSSRVQLPPGQYLVVPSTFEPFKDGDFCLRVFSEKK
                     AQALEIGDAVAGDPHEPHPRDMDGEDEHFWSLSEEFADKDSEISAHQLKRVLNGLLSK
                     RTDMKFDGFNINTCREMISLLDGDGTGSLRPVEFKTLWLKICKYLEIYQEMDHSRAGT
                     IDAHEMRTALKKAGFTLNNQVQQTIATRYACSKLGVDFDGFVACMIRLEILFKLFRLL
                     DKDQNGIVQLSLAEWLCCALV"
     exon            292..361
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            362..480
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            481..614
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            615..783
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            784..867
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            868..953
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1028
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1189
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1365
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1377
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1589
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1626
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1695
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1753
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..1818
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1887
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..1966
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            1967..2083
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2142
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
     exon            2143..2411
                     /gene="Capn8"
                     /gene_synonym="nCL-2; nCL-2'"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagaggacac agaagccctg ctttcctgtc acctacagga cgcacaggca ggccatggca
       61 gccctggcag ctggaatatc caagcaacgt gcagctgctc agggccttgg ctctaaccag
      121 aatgctgtga agtatctggg ccaggacttc gagaccctga gaaagcaatg cttgaactca
      181 ggggtcctat ttaaagaccc ggaattcccg gcatgtccgt cagctttggg ctacagggat
      241 cttggaccag gctctgcaga aactcaaggc atcatatgga agcgacccac ggaattgtgt
      301 tccaaccctc agtttatcgt tggtggagcc acacgcacag acatccgcca ggggggtctt
      361 ggagactgtt ggcttctggc agccattgcc tccctcacct tgaatgaaaa gctgctttat
      421 cgagtggttc ctagagacca gagcttccag aagaactatg ctggcatttt tcatttccag
      481 ttctggcagt atggagagtg ggtggaggtg gtcattgatg accggctccc caccaagaat
      541 ggacagctgc tcttcctaca ctccgaagaa ggcaatgagt tctggagtgc tctgctggag
      601 aaagcctatg ccaagctcaa tggttcgtat gaggctcttg ctggaggctc cacaattgag
      661 gggtttgagg atttcacggg tggcatctct gagttttatg acttgaggaa gcctccgggc
      721 aatctgtact acaccatcca gaaggccctg cgcaaaggct ctctgctggg ctgctccatt
      781 gatgtctcaa acgcagccga agcagaagcc accaccaggc aaaagctggt taagggccac
      841 gcatactctg tgactggagt cgaagaggtg gatttccgtg gcctcccaga gaagctgatc
      901 aggttgagga acccgtgggg tgaagtggag tggacgggag cctggagtga tagtgcacct
      961 gagtggaatt acatagatcc acagaagaag ggagaactgg acaagagagc agaagatggg
     1021 gagttctgga tgtccttttc ggatttcttg aagcagttct cccgactaga gatatgcaac
     1081 ctgtccccag actctctgag cagtgaggag atacacaaat ggaacctggt actcttcaac
     1141 ggccgctgga cacggggttc tacagccggg ggctgccaga actacccagc cacttactgg
     1201 accaaccccc agtttaaaat ccatttggac gaggtggatg aggaccagga ggagggcacc
     1261 agtgaaccct gctgtacagt gctgctgggt ctgatgcaga agaatcgcag acgtcagagg
     1321 aggatcggcc agggcatgct cagcataggc tatgctgtct accagattcc caaggagctg
     1381 gagaatcaca ccgatgagca cctgggccgg gatttcttcc agggccgcca gccctccacc
     1441 tgctccagca cctacatgaa ccttcgagag gtgtccagca gggtccaact ccccccagga
     1501 cagtacctgg tggtgccatc cacctttgag cccttcaagg acggtgactt ctgcctgagg
     1561 gtgttctcgg agaagaaggc ccaggctcta gaaattgggg acgctgttgc tggagaccca
     1621 catgagccac atccccgtga catggatgga gaagatgagc acttctggag cctgtctgag
     1681 gagtttgcgg acaaggattc tgaaatcagt gctcaccagc tcaagagggt cctgaatgga
     1741 ttgctttcta aacgaacaga catgaaattt gacggattca acatcaacac ttgcagagaa
     1801 atgatcagcc tgctggatgg tgatgggaca ggaagcctgc gaccggtgga gttcaagact
     1861 ctctggctga agatctgcaa gtatctggag atctaccaag aaatggacca cagccgtgca
     1921 gggaccattg atgctcacga gatgaggaca gctctcaaga aagcaggttt caccctcaac
     1981 aaccaggtgc agcagaccat tgccacgagg tatgcatgca gcaagcttgg tgttgacttt
     2041 gacggctttg tggcttgtat gatccgcctg gagatcctgt tcaaattgtt cagactcttg
     2101 gataaggacc agaatggcat tgtacagctc tccctggctg agtggctgtg ctgtgcactg
     2161 gtctgaccct ctggacatca acaacttccc tgcttccaac ctgtctcttt gcaatcttat
     2221 gaacgtgtat cctcaagtgt catttactga ctgttgtatt gtccagccaa tcactgatcc
     2281 cgagacactg gcttttcccg atagagcagg ctggggagcc ccatatcctc agtagcaccg
     2341 agctacgagc tatttgagct gatcccaggg cccagcagaa ggaaagcaat caattaaagt
     2401 tgtgggccag g
//
