LOCUS       NM_007602               4515 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus calpain 5 (Capn5), transcript variant 1, mRNA.
ACCESSION   NM_007602
VERSION     NM_007602.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Chukai,Y., Ito,G., Miki,Y., Wakabayashi,K., Itoh,K., Sugano,E.,
            Tomita,H., Fukuda,T. and Ozaki,T.
  TITLE     Role of calpain-5 in cerebral ischemia and reperfusion injury
  JOURNAL   Biochim Biophys Acta Gen Subj 1868 (1), 130506 (2024)
   PUBMED   37949151
  REMARK    GeneRIF: Role of calpain-5 in cerebral ischemia and reperfusion
            injury.
REFERENCE   3  (bases 1 to 4515)
  AUTHORS   Chukai,Y., Iwamoto,T., Itoh,K., Tomita,H. and Ozaki,T.
  TITLE     Characterization of mitochondrial calpain-5
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1868 (5), 118989 (2021)
   PUBMED   33607190
  REMARK    GeneRIF: Characterization of mitochondrial calpain-5.
REFERENCE   4  (bases 1 to 4515)
  AUTHORS   Gafni,J., Hermel,E., Young,J.E., Wellington,C.L., Hayden,M.R. and
            Ellerby,L.M.
  TITLE     Inhibition of calpain cleavage of huntingtin reduces toxicity:
            accumulation of calpain/caspase fragments in the nucleus
  JOURNAL   J Biol Chem 279 (19), 20211-20220 (2004)
   PUBMED   14981075
  REMARK    GeneRIF: calpain-derived Htt fragmentation has a role in
            Huntington's disease and aberrant activation of calpains may play a
            role in HD pathogenesis
REFERENCE   5  (bases 1 to 4515)
  AUTHORS   Franz,T., Winckler,L., Boehm,T. and Dear,T.N.
  TITLE     Capn5 is expressed in a subset of T cells and is dispensable for
            development
  JOURNAL   Mol Cell Biol 24 (4), 1649-1654 (2004)
   PUBMED   14749380
  REMARK    GeneRIF: Despite the numerous expression sites of Capn5, the
            majority of Capn5-null mice were viable and fertile and appeared
            healthy
REFERENCE   6  (bases 1 to 4515)
  AUTHORS   Dear,T.N. and Boehm,T.
  TITLE     Diverse mRNA expression patterns of the mouse calpain genes Capn5,
            Capn6 and Capn11 during development
  JOURNAL   Mech Dev 89 (1-2), 201-209 (1999)
   PUBMED   10559499
REFERENCE   7  (bases 1 to 4515)
  AUTHORS   Matena,K., Boehm,T. and Dear,N.
  TITLE     Genomic organization of mouse Capn5 and Capn6 genes confirms that
            they are a distinct calpain subfamily
  JOURNAL   Genomics 48 (1), 117-120 (1998)
   PUBMED   9503024
REFERENCE   8  (bases 1 to 4515)
  AUTHORS   Li,J., Nixon,R., Messer,A., Berman,S. and Bursztajn,S.
  TITLE     Altered gene expression for calpain/calpastatin system in motor
            neuron degeneration (Mnd) mutant mouse brain and spinal cord
  JOURNAL   Brain Res Mol Brain Res 53 (1-2), 174-186 (1998)
   PUBMED   9473662
REFERENCE   9  (bases 1 to 4515)
  AUTHORS   Sorimachi,H., Ishiura,S. and Suzuki,K.
  TITLE     Structure and physiological function of calpains
  JOURNAL   Biochem J 328 (Pt 3) (Pt 3), 721-732 (1997)
   PUBMED   9396712
  REMARK    Review article
REFERENCE   10 (bases 1 to 4515)
  AUTHORS   Dear,N., Matena,K., Vingron,M. and Boehm,T.
  TITLE     A new subfamily of vertebrate calpains lacking a calmodulin-like
            domain: implications for calpain regulation and evolution
  JOURNAL   Genomics 45 (1), 175-184 (1997)
   PUBMED   9339374
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY794575.1, AK164293.1,
            AK139476.1, AI049109.1 and BQ176356.1.
            
            On Jul 30, 2014 this sequence version replaced NM_007602.3.
            
            Summary: The protein encoded by this gene is a member of the
            calpain family of proteins. Unlike many members of the calpain gene
            family, this gene lacks a calmodulin-like domain, required for
            calcium binding. Mouse models for Huntington's disease displayed
            increased levels of the protein encoded by this gene. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK155179.1, AK170034.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-91                BY794575.1         1-91
            92-2420             AK164293.1         2-2330
            2421-3548           AK139476.1         570-1697
            3549-3549           AI049109.1         283-283
            3550-3903           AK139476.1         1699-2052
            3904-4515           BQ176356.1         1-612               c
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 53.57 cM"
     gene            1..4515
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /note="calpain 5"
                     /db_xref="GeneID:12337"
                     /db_xref="MGI:MGI:1100859"
     exon            1..231
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            232..426
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     CDS             262..2184
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /note="new calpain 3"
                     /codon_start=1
                     /product="calpain-5"
                     /protein_id="NP_031628.1"
                     /db_xref="CCDS:CCDS21465.1"
                     /db_xref="GeneID:12337"
                     /db_xref="MGI:MGI:1100859"
                     /translation="MFSCAKAYEDQNYSALKRACLRKKVLFEDPLFPATDDSLYYKGT
                     PGPTVRWKRPKDICDDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIP
                     DWKEQEWNPEKPDSYAGIFHFNFWRFGEWVDVIVDDRLPTVNNQLIYCHSNSKNEFWC
                     ALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDLATDEAKRNQLFERV
                     LKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLGHGLLAFFKSEK
                     LDMIRLRNPWGEREWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYF
                     TDIIKCRLINTSYLSIHKTWEEARLHGAWTRHEDPQQNRSGGCINHKDTFFQNPQYVF
                     EVKKPEDEVLISIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSI
                     YINSRSVFLRTELPEGRYVIIPTTFEPGHTGEFLLRVFTDVPSNCRELRLDEPPRTCW
                     SSLCGYPQQVAQVHVLGAAGLKDSPTGANSYVIIKCEGEKVRSAVQRGTSTPEYNVKG
                     IFYRKKLAQPITVQVWNHRVLKDEFLGQVHLKTAPDDLQDLHTLHLQDRSSRQPSDLP
                     GIVAVRVLCSASLTAV"
     exon            427..558
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            559..767
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            768..960
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1154
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1232
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1428
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1551
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1748
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1864
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..2001
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..4498
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4471..4476
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /note="hexamer: AATAAA"
     polyA_site      4492
                     /gene="Capn5"
                     /gene_synonym="nCL-3"
                     /note="major polyA site"
ORIGIN      
        1 gccccacccc gggagaccac gcctctggcc tccaatggga ggaggctgag aggaggggcg
       61 ggacagactg aagcccgggg gtccaagttc caacccccgc ctgcgggctg ccggggtatc
      121 atctccccgc agagtcccag gctgtggcgc gggctggtct agcctccgct ccagtgcccg
      181 cactgtgctc tgcatcccgg gagtccagct ccagctgcgg cgacgcggca ggtgcctccc
      241 cttcttgggg acgtggtcac catgttctcc tgcgcgaagg cctatgagga ccagaactac
      301 tcggcgctga agcgggcctg cctgcgcaag aaggtgctgt tcgaggatcc cctcttccct
      361 gccaccgacg actcccttta ctataagggc accccagggc ccacagtcag gtggaagcgg
      421 cctaaggata tctgcgacga tccccggctc ttcgtagatg gcatcagctc ccatgacctg
      481 caccagggcc aggtgggcaa ctgctggttt gtggctgcct gctcatcact ggcctcccga
      541 gagtcactct ggcagaaggt catcccagac tggaaggagc aggaatggaa ccccgagaag
      601 cctgacagct atgctggcat cttccacttc aacttctggc gctttgggga gtgggtggac
      661 gtaatcgtcg atgaccggct gcccacagtc aacaaccagc tcatttactg ccattccaac
      721 tccaaaaatg agttctggtg tgccctggtg gagaaggcct atgccaagct ggccggctgt
      781 taccaggccc tggacggagg caacacggcc gatgcattgg tggatttcac aggtggtgtt
      841 tctgaaccca ttgacctgac cgagggggac ttggccactg acgaggctaa gaggaatcag
      901 ctctttgagc gagtgctgaa ggtgcacagc agaggcgggc tcatcagtgc ctccatcaag
      961 gctgtgacag cagctgacat ggaggcccgc ctggcatgtg gcctggtgaa gggccatgca
     1021 tacgctgtca ccgatgtgcg caaggtgcgc ctgggccatg gcctgctggc cttcttcaag
     1081 tcagagaagc ttgatatgat ccgtctgagg aacccctggg gcgagcggga gtggacgggg
     1141 ccctggagtg acacgtcaga ggaatggcag aaagtgagca agagtgagag ggagaagatg
     1201 ggcgtgaccg tgcaggatga tggggaattc tggatgacct ttgaggacat gtgccggtac
     1261 tttactgaca tcattaaatg ccgcctgatt aacacgtcct acctgagcat ccataagaca
     1321 tgggaggagg cccggctgca tggtgcctgg acgagacatg aggacccaca gcagaaccgc
     1381 agtggaggct gcatcaacca caaggacact ttcttccaga acccacagta cgtatttgaa
     1441 gtcaagaagc cagaagatga agtgttgatc agtatccagc agcggccgaa gcgctcaact
     1501 cgccgggagg gcaaaggcga gaatctggcc attggcttcg acatctataa ggtggaagag
     1561 aaccgccaat accgtatgca cagcctacag cataaggccg ccagctccat ctacatcaat
     1621 tcccgcagcg tttttttgag gacagagctg cccgagggcc gctacgttat catccctacc
     1681 acctttgagc caggccacac tggcgagttc ctgctccgag tcttcacaga tgtcccctcc
     1741 aactgccggg aactacgcct ggatgagccc cctcggacct gttggagttc cctctgtggc
     1801 taccctcagc aggtggccca ggtacatgtc ctgggggctg ctggcctcaa ggactcccca
     1861 acaggagcaa actcatatgt gatcatcaag tgtgagggcg aaaaggttcg ctcagctgtg
     1921 cagagaggga cctcgacacc agagtacaat gtaaaaggca tcttctatcg caagaagctg
     1981 gctcagccta tcaccgtgca ggtttggaat caccgagtcc tgaaggatga attcctgggc
     2041 caggtgcacc tgaagactgc cccggatgac ctgcaggacc tccacaccct ccatctccag
     2101 gaccgcagta gccggcagcc cagtgacctg ccaggcattg tagctgtgcg agtcctctgc
     2161 agtgcctctc tcacggctgt ctgaccccag cctgcctgtc ctgccccact agtcctcacc
     2221 actactcgca tgtccccacc ttgcctggga ccagcctggg aaccagacac tggggccctt
     2281 tcctcactct tccactgacc cactgtgtga cctgaagaga gccctgccct ctctgagcct
     2341 cagtgtttgg agggccccaa agaattcccg tcttgtgggg gagttttctt gcctaagatt
     2401 taatgcagtt ctctctaccc agtgggcgct gctgttaagg ggccatctgc tgaaaacgtt
     2461 tccccaggcc ctgctgtctg ccaggagtgc caagtgtcaa ctgtttacac acaaactgcc
     2521 atgtcccacg ccccttccct ctccttgttt gctatggggg agattgcttc cctccccaga
     2581 tctttgctat gtagcttaga gtgaccttaa acttatgatc cccctgcctc cacctcctca
     2641 atgccgtacc actataccag tttgagtggt gctgggtatg gaacccaggg ctttgtgcat
     2701 gctaggcgag cacttaccaa ctgagctaca tccccaatcc cactacagcc cattttaaaa
     2761 ccatcttgaa agaaccctta ttggcatatg caggagacat ctgctcattg tctaatgcct
     2821 gacccccatt catttagtta ttcaatatag acttgcccaa ttagcccatc gagcctccct
     2881 aggcttcccg attctcaggt cttctcggag gcagatccct tcctgagctc caggctctga
     2941 cttcaccgtc accctctgaa gagctatgat catgctggtg agaaagtatg atttcccaac
     3001 ctctgcttgt ggtgttggaa ggagtggcct ggccaggcag gttgatgtaa ggctatggga
     3061 gacccaaggg gaacacatgc tgcctctgct gaaagaccct gggtttttct tggaagaaag
     3121 gactctaggg tggatggtac caggccttca gggttagatc atctgtcaag tgtcaccgat
     3181 ggggacttct catgaccaga gggctctgca ccagcatccc caaagagcca agaaggccac
     3241 catgctttta ctgtccagac caaaatgttg ctctcaatcc ccaacatttg atagtcttag
     3301 catttaaaca aaatctccga gtgagggagg ccagccccca catttgtcct ctgcaggctc
     3361 attttgttga gcgtctgctt ttatcttgac actgagcacg agagggcacg ggtgggtgct
     3421 cacagtggga ccttcttgga ggaatctcag agggggacag agatgagatg tgcattcctg
     3481 gctgccacct gcattcctga ggaactcttg tttactggtg ctgtggctca caggtccccc
     3541 tctgtgccct ccttcagctt ccaggccaga cttagtagat aaatagtaat aataataata
     3601 atacaattga gttatcagcc acaattcctt ttggaactgt tacactctgc ctctggtccc
     3661 tccaacgtgg tcctcttcct ggctggaggt gctggattct gactgccatc ttgctccatg
     3721 gtggctcttc tggcctggga cccggtccac aggaagggct aggagctgaa ttaaagatga
     3781 gagcatccct cctgcacact ggggcttccc ttcaccagga gcaaatcact tcaacctccc
     3841 gtgcctcgtt tcctccctct gtagcatggg gctcgagact cctgtttgtg agggactctt
     3901 acaaggataa gagcaaggcc acaccacttc cgctgtgtat cagtctatca tacactccgc
     3961 acggtcacat gatcacttca cttctcgatg gctattgcag accaagactg cagtcaagaa
     4021 gggagggtga gcctcacgat cttgggtctc agggcctgac ataggggtct tagctcagcc
     4081 atatccctct gaggcacagg gcgagcaggg accaagtagc agacgagtcg atgcttcacc
     4141 tggcatctag ccactgcctc ccacttctag cagaactggg tcagcagccc tgttcttgct
     4201 tcctctctct taagctaggc caggctggct cctggctgtg aaggaacttc cggtccccaa
     4261 gaagccttga agactggaca agggaggaca gggtggagca gggatagccg tggcttcatt
     4321 tatcgtttgc acccaggagg ccttgtagtc ttccaattgc aggagatcag ccctacagtg
     4381 tattctacgt gaaccaacag ggcctggcct tcactcctcc aggccacaat gccctggggt
     4441 caagggaaag gaactctgtt atcttctcag aataaatatt actgccgctc tagagggaaa
     4501 aaaaaaaaaa aaaaa
//
