LOCUS       NM_007601               3167 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus calpain 3 (Capn3), transcript variant a, mRNA.
ACCESSION   NM_007601
VERSION     NM_007601.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Cao,R., Liu,Y., Wei,K., Jin,N., Liang,Y., Ao,R., Pan,W., Wang,X.,
            Wang,X., Zhang,L. and Xie,J.
  TITLE     Genes related to neural tube defects and glioblastoma
  JOURNAL   Sci Rep 15 (1), 3777 (2025)
   PUBMED   39885289
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Villani,K.R., Zhong,R., Henley-Beasley,C.S., Rastelli,G.,
            Harris,E., Boncompagni,S., Barton,E.R. and Wei-LaPierre,L.
  TITLE     Loss of Calpain 3 dysregulates store-operated calcium entry and its
            exercise response in mice
  JOURNAL   FASEB J 38 (14), e23825 (2024)
   PUBMED   39031532
  REMARK    GeneRIF: Loss of Calpain 3 dysregulates store-operated calcium
            entry and its exercise response in mice.
REFERENCE   3  (bases 1 to 3167)
  AUTHORS   Ma,H.S., Gong,X.L., Li,W.X., Cai,Q., Chen,Y.W., Guo,X.B., Ren,Z.R.,
            Zeng,F. and Yan,J.B.
  TITLE     Missense mutation of c.635 T > C in CAPN3 impairs muscle injury
            repair in a Limb-Girdel Muscular Dystropy Model
  JOURNAL   Clin Genet 103 (6), 663-671 (2023)
   PUBMED   36999564
  REMARK    GeneRIF: Missense mutation of c.635 T > C in CAPN3 impairs muscle
            injury repair in a Limb-Girdel Muscular Dystropy Model.
REFERENCE   4  (bases 1 to 3167)
  AUTHORS   Winter,L., Kustermann,M., Ernhofer,B., Hoger,H., Bittner,R.E. and
            Schmidt,W.M.
  TITLE     Proteins implicated in muscular dystrophy and cancer are functional
            constituents of the centrosome
  JOURNAL   Life Sci Alliance 5 (11), e202201367 (2022)
   PUBMED   35790299
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3167)
  AUTHORS   Lasa-Elgarresta,J., Mosqueira-Martin,L., Gonzalez-Imaz,K.,
            Marco-Moreno,P., Gerenu,G., Mamchaoui,K., Mouly,V., Lopez de
            Munain,A. and Vallejo-Illarramendi,A.
  TITLE     Targeting the Ubiquitin-Proteasome System in Limb-Girdle Muscular
            Dystrophy With CAPN3 Mutations
  JOURNAL   Front Cell Dev Biol 10, 822563 (2022)
   PUBMED   35309930
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3167)
  AUTHORS   Sorimachi,H., Forsberg,N.E., Lee,H.J., Joeng,S.Y., Richard,I.,
            Beckmann,J.S., Ishiura,S. and Suzuki,K.
  TITLE     Highly conserved structure in the promoter region of the gene for
            muscle-specific calpain, p94
  JOURNAL   Biol Chem 377 (12), 859-864 (1996)
   PUBMED   8997499
REFERENCE   7  (bases 1 to 3167)
  AUTHORS   Spencer,M.J. and Tidball,J.G.
  TITLE     Calpain translocation during muscle fiber necrosis and regeneration
            in dystrophin-deficient mice
  JOURNAL   Exp Cell Res 226 (2), 264-272 (1996)
   PUBMED   8806430
REFERENCE   8  (bases 1 to 3167)
  AUTHORS   Li,J., Grynspan,F., Berman,S., Nixon,R. and Bursztajn,S.
  TITLE     Regional differences in gene expression for calcium activated
            neutral proteases (calpains) and their endogenous inhibitor
            calpastatin in mouse brain and spinal cord
  JOURNAL   J Neurobiol 30 (2), 177-191 (1996)
   PUBMED   8738748
REFERENCE   9  (bases 1 to 3167)
  AUTHORS   Richard,I. and Beckmann,J.S.
  TITLE     Molecular cloning of mouse canp3, the gene associated with
            limb-girdle muscular dystrophy 2A in human
  JOURNAL   Mamm Genome 7 (5), 377-379 (1996)
   PUBMED   8661728
REFERENCE   10 (bases 1 to 3167)
  AUTHORS   Ohno,S., Minoshima,S., Kudoh,J., Fukuyama,R., Shimizu,Y.,
            Ohmi-Imajoh,S., Shimizu,N. and Suzuki,K.
  TITLE     Four genes for the calpain family locate on four distinct human
            chromosomes
  JOURNAL   Cytogenet Cell Genet 53 (4), 225-229 (1990)
   PUBMED   2209092
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY142198.1, CN664345.1, BC139790.1, AL935121.9, BC090661.1 and
            BB202194.1.
            
            On Apr 28, 2010 this sequence version replaced NM_007601.2.
            
            Transcript Variant: This variant (a) is the longest transcript and
            it encodes the longest protein (isoform a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC139790.1, X92523.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-323               BY142198.1         1-323
            324-580             CN664345.1         304-560
            581-1101            BC139790.1         301-821
            1102-1102           AL935121.9         81251-81251
            1103-1348           BC139790.1         823-1068
            1349-2062           BC090661.1         938-1651
            2063-2673           BC139790.1         1783-2393
            2674-3154           BC090661.1         2131-2611
            3155-3167           BB202194.1         204-216
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.31 cM"
     gene            1..3167
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /note="calpain 3"
                     /db_xref="GeneID:12335"
                     /db_xref="MGI:MGI:107437"
     exon            1..589
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     CDS             281..2746
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /EC_number="3.4.22.54"
                     /note="isoform a is encoded by transcript variant a;
                     skeletal muscle specific calpain p94; CANP 3; calpain L3;
                     calcium-activated neutral proteinase 3; muscle-specific
                     calcium-activated neutral protease 3"
                     /codon_start=1
                     /product="calpain-3 isoform a"
                     /protein_id="NP_031627.2"
                     /db_xref="CCDS:CCDS16620.1"
                     /db_xref="GeneID:12335"
                     /db_xref="MGI:MGI:107437"
                     /translation="MPTVISPTVAPRTGAEPRSPGPVPHPAQGKTTEAGGGHPSGIYS
                     AIISRNFPIIGVKEKTFEQLRRKCLEKKVLYLDPEFPPDETSLFYSQKFPIQFVWKRP
                     PEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYA
                     GIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEA
                     LKGGNTTEAMEDFTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDDGTNMTYGT
                     SPSGLNMGELIARMVRNMDNSLLRDSDLDPRGSDDRPSRTIVPVQYETRMACGLVKGH
                     AYSVTGLEEALFKGEKVKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKDEKARLQHQVT
                     EDGEFWMSYDDFVYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNF
                     PDTFWTNPQYRLKLLEEDDDPEDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYE
                     VPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQE
                     GEFILRVFSEKRNLSEEAENTISVDRPVKKKKNKPIIFVSDRANSNKELGVDQEAEEG
                     KDKAGPEKRGETPQPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVN
                     KHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSG
                     TINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFNA
                     FDKDGDGIIKLNVLEWLQLTMYA"
     exon            590..659
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            660..778
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            779..912
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1081
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1225
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1309
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1395
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1473
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1634
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1804
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1816
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..2025
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2062
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2080
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2194
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..2272
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2273..2330
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2331..2395
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2396..2464
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2465..2543
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2544..2660
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2661..2719
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..3166
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3133..3138
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /note="hexamer: AATAAA"
     polyA_site      3160
                     /gene="Capn3"
                     /gene_synonym="Capa-3; Capa3; Lp82; p94"
                     /note="major polyA site"
ORIGIN      
        1 tggcaagtgc gctgctgaag gccactaggt agaaactgcg tctaaatcct ttattgactc
       61 ttcctcagga aaacctatcg ctctagggtc atagttcact atttgagctg gtcagaagcc
      121 agtcagtttt ctgataggat ttaaactttg aagagactgt ggccattttt ttcctcagat
      181 gacagaatca cttcaacttc agctttgtaa tcacttcctt tccttgaagg tagctgaatc
      241 tcattttctt taaaaagtct tccttccaaa gttgcctgcc atgccaactg ttattagtcc
      301 aactgtggcc ccaaggacgg gggctgagcc caggtcccca gggccagttc ctcacccagc
      361 tcaaggcaag accaccgagg ctggaggtgg acacccaagt ggcatctatt cggccatcat
      421 tagccgcaat tttccgatca tcggtgtgaa agagaagaca tttgagcagc tccgcaggaa
      481 gtgcctagag aagaaagttc tttatctaga ccccgagttc ccaccggatg agacctctct
      541 cttttacagt cagaagttcc ccatccagtt cgtctggaag agacctccgg aaatttgtga
      601 gaatccccgg tttatcattg gtggagccaa caggactgac atctgccaag gagatctagg
      661 ggactgctgg tttcttgcag ccatcgcctg cctgaccctg aatgagcggc tgcttttccg
      721 agttataccc catgatcaaa gtttcactga aaactacgca gggatcttcc acttccagtt
      781 ctggcgctat ggagactggg tagatgtggt cattgacgac tgtctgccaa catacaacaa
      841 tcagctggtt ttcaccaaat ccaaccaccg caatgagttc tggagtgctc tccttgagaa
      901 ggcttatgcc aagctccatg gctcctatga agctctgaaa ggtgggaata ccacagaagc
      961 catggaggac ttcacaggag gggtgacaga gttttttgag atcaaggatg ctccgagtga
     1021 catgtacaag attatgagga aagctatcga gagaggctcc ctcatgggct gctccattga
     1081 cgatggcacg aacatgactt atggaacctc tccttctggt ctgaacatgg gggagttgat
     1141 tgcacggatg gtgagaaata tggataactc gctgctcaga gactcagacc tggaccccag
     1201 gggctcagat gacagaccgt cacggacaat tgttccagtg cagtatgaaa caagaatggc
     1261 ctgtgggttg gtgaaagggc atgcctattc agtcactggg ctggaggagg ccctgttcaa
     1321 aggtgagaag gtgaagttgg tgcggctgcg gaacccctgg ggccaggtgg agtggaacgg
     1381 ctcttggagt gatggttgga aggactggag ctttgtagac aaagatgaga aggcccgtct
     1441 acagcaccag gttactgagg atggagagtt ctggatgtca tatgatgact tcgtctacca
     1501 tttcacgaag ctggagatct gcaacctcac agctgacgcc ctggagtccg ataagcttca
     1561 gacctggacg gtgtctgtaa acgagggccg ctgggtgagg ggctgttctg ctggaggctg
     1621 ccgaaacttc ccagacactt tctggaccaa cccgcagtac cgtctcaagc ttctggagga
     1681 agacgatgac cctgaggact ctgaggtaat ctgcagcttc ctcgtggctc tgatgcagaa
     1741 gaaccggcgc aaggaccgga agctgggggc caacctcttc actattggct tcgccatcta
     1801 cgaggttccc aaagagatgc acgggaataa gcaacacctg cagaaggact tcttcttgta
     1861 caacgcctcc aaggccagaa gcaaaaccta catcaacatg cgggaggtgt cccagcgctt
     1921 ccgcctgcca cccagcgagt atgtcatcgt accctccacc tacgagcccc accaggaggg
     1981 ggaattcatc ctccgagtct tctccgaaaa gaggaatctc tctgaggaag ctgaaaatac
     2041 aatctctgtg gatcggccag tgaaaaagaa aaaaaacaag cccatcatct tcgtttctga
     2101 cagagcaaac agcaacaagg agctgggtgt ggaccaggag gcagaggagg gcaaagacaa
     2161 agcagggccc gagaaacgag gggaaacccc acagccaagg cctggccaca cagaccagga
     2221 aagtgaggag cagcagcaat tccggaacat cttcaggcag atcgcaggcg acgacatgga
     2281 gatctgtgca gatgaactca agaatgtcct caacacagtg gtgaacaaac acaaggacct
     2341 gaagacacaa gggttcacgc tggagtcctg cagaagcatg atagctctca tggatacaga
     2401 tggctctgga agactgaatc ttcaagagtt ccatcacctc tggaaaaaga tcaaggcctg
     2461 gcagaaaatc ttcaagcact atgacacaga ccattccggt accatcaata gctatgagat
     2521 gcgaaatgca gtcaatgatg caggcttcca tctcaacagc caactctatg acatcatcac
     2581 catgcgctat gcggacaaac acatgaacat cgactttgac agcttcatct gctgcttcgt
     2641 caggctggaa gggatgttca gagcttttaa cgcatttgac aaggatggcg atggtatcat
     2701 caaactgaat gtacttgagt ggctgcagct taccatgtat gcctgaacca gactgacctc
     2761 atgttaagag caactaggat ttcatctcaa cacaacacag ctagagccat ctaccacaaa
     2821 agacccagta ggtgcatctc caccaaacct agggggctcc tggtcatgtt ccttctccat
     2881 tttgcaccaa ctcttggtgc acagcctgat gccacctgaa gtgtctggct tgctgagagg
     2941 tctgtagatt ctgaaactgt ctacttagct tgtactgggt tgtccatagc acctgtagat
     3001 ggctctcact cacggaggag cccagtcgcc atctgcactc agttctgcca cttttagggt
     3061 gggtctattc tggggtcaat agggcgtgga tatctgacaa aaatgtggct acattctgaa
     3121 agaagctatc taaataaagg cacacacatg gctagttcca ccattca
//
