LOCUS       NM_009794               3330 bp    mRNA    linear   ROD 03-DEC-2024
DEFINITION  Mus musculus calpain 2 (Capn2), mRNA.
ACCESSION   NM_009794 XM_977623
VERSION     NM_009794.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Lahiri,S.K., Lu,J., Aguilar-Sanchez,Y., Li,H., Moreira,L.M.,
            Hulsurkar,M.M., Mendoza,A., Turkieltaub Paredes,M.R.,
            Navarro-Garcia,J.A., Munivez,E., Horist,B., Moore,O.M.,
            Weninger,G., Brandenburg,S., Lenz,C., Lehnart,S.E., Sayeed,R.,
            Krasopoulos,G., Srivastava,V., Zhang,L., Karch,J.M., Reilly,S. and
            Wehrens,X.H.T.
  TITLE     Targeting calpain-2-mediated junctophilin-2 cleavage delays heart
            failure progression following myocardial infarction
  JOURNAL   J Mol Cell Cardiol 194, 85-95 (2024)
   PUBMED   38960317
  REMARK    GeneRIF: Targeting calpain-2-mediated junctophilin-2 cleavage
            delays heart failure progression following myocardial infarction.
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Muller,T., Reichlmeir,M., Hau,A.C., Wittig,I. and Schulte,D.
  TITLE     The neuronal transcription factor MEIS2 is a calpain-2 protease
            target
  JOURNAL   J Cell Sci 137 (4) (2024)
   PUBMED   38305737
  REMARK    GeneRIF: The neuronal transcription factor MEIS2 is a calpain-2
            protease target.
REFERENCE   3  (bases 1 to 3330)
  AUTHORS   Wang,L.H., Lin,C.Y., Lin,Y.M., Buee,L., Sergeant,N., Blum,D.,
            Chern,Y. and Wang,G.S.
  TITLE     Calpain-2 Mediates MBNL2 Degradation and a Developmental RNA
            Processing Program in Neurodegeneration
  JOURNAL   J Neurosci 42 (25), 5102-5114 (2022)
   PUBMED   35606145
  REMARK    GeneRIF: Calpain-2 Mediates MBNL2 Degradation and a Developmental
            RNA Processing Program in Neurodegeneration.
REFERENCE   4  (bases 1 to 3330)
  AUTHORS   Liu,G.T., Kochlamazashvili,G., Puchkov,D., Muller,R., Schultz,C.,
            Mackintosh,A.I., Vollweiter,D., Haucke,V. and Soykan,T.
  TITLE     Endosomal phosphatidylinositol 3-phosphate controls synaptic
            vesicle cycling and neurotransmission
  JOURNAL   EMBO J 41 (9), e109352 (2022)
   PUBMED   35318705
REFERENCE   5  (bases 1 to 3330)
  AUTHORS   Muniappan,L., Okuyama,M., Javidan,A., Thiagarajan,D., Jiang,W.,
            Moorleghen,J.J., Yang,L., Balakrishnan,A., Howatt,D.A.,
            Uchida,H.A., Saido,T.C. and Subramanian,V.
  TITLE     Inducible Depletion of Calpain-2 Mitigates Abdominal Aortic
            Aneurysm in Mice
  JOURNAL   Arterioscler Thromb Vasc Biol 41 (5), 1694-1709 (2021)
   PUBMED   33761765
  REMARK    GeneRIF: Inducible Depletion of Calpain-2 Mitigates Abdominal
            Aortic Aneurysm in Mice.
REFERENCE   6  (bases 1 to 3330)
  AUTHORS   Sorimachi,H., Ishiura,S. and Suzuki,K.
  TITLE     Structure and physiological function of calpains
  JOURNAL   Biochem J 328 (Pt 3) (Pt 3), 721-732 (1997)
   PUBMED   9396712
  REMARK    Review article
REFERENCE   7  (bases 1 to 3330)
  AUTHORS   Dear,N., Matena,K., Vingron,M. and Boehm,T.
  TITLE     A new subfamily of vertebrate calpains lacking a calmodulin-like
            domain: implications for calpain regulation and evolution
  JOURNAL   Genomics 45 (1), 175-184 (1997)
   PUBMED   9339374
REFERENCE   8  (bases 1 to 3330)
  AUTHORS   Spencer,M.J. and Tidball,J.G.
  TITLE     Calpain translocation during muscle fiber necrosis and regeneration
            in dystrophin-deficient mice
  JOURNAL   Exp Cell Res 226 (2), 264-272 (1996)
   PUBMED   8806430
REFERENCE   9  (bases 1 to 3330)
  AUTHORS   Li,J., Grynspan,F., Berman,S., Nixon,R. and Bursztajn,S.
  TITLE     Regional differences in gene expression for calcium activated
            neutral proteases (calpains) and their endogenous inhibitor
            calpastatin in mouse brain and spinal cord
  JOURNAL   J Neurobiol 30 (2), 177-191 (1996)
   PUBMED   8738748
REFERENCE   10 (bases 1 to 3330)
  AUTHORS   Ohno,S., Minoshima,S., Kudoh,J., Fukuyama,R., Shimizu,Y.,
            Ohmi-Imajoh,S., Shimizu,N. and Suzuki,K.
  TITLE     Four genes for the calpain family locate on four distinct human
            chromosomes
  JOURNAL   Cytogenet Cell Genet 53 (4), 225-229 (1990)
   PUBMED   2209092
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC131742.2, BC054726.1 and BG091141.1.
            
            On Feb 13, 2018 this sequence version replaced NM_009794.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC054726.1, AK154855.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               AC131742.2         184339-184465       c
            128-3312            BC054726.1         1-3185
            3313-3330           BG091141.1         233-250
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 84.93 cM"
     gene            1..3330
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /note="calpain 2"
                     /db_xref="GeneID:12334"
                     /db_xref="MGI:MGI:88264"
     exon            1..493
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     CDS             257..2359
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /EC_number="3.4.22.53"
                     /note="cysteine proteinase; m-calpain 80 kDa large
                     subunit; m-calpain large subunit; CANP 2; calpain M-type;
                     millimolar-calpain; calpain-2 large subunit; 80 kDa
                     M-calpain subunit; calcium-activated neutral proteinase 2"
                     /codon_start=1
                     /product="calpain-2 catalytic subunit"
                     /protein_id="NP_033924.2"
                     /db_xref="CCDS:CCDS35813.1"
                     /db_xref="GeneID:12334"
                     /db_xref="MGI:MGI:88264"
                     /translation="MAGIAIKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAGA
                     LFQDPSFPALPSSLGYKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGAL
                     GDCWLLAAIASLTLNEEILARVVPPDQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPT
                     KDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELR
                     KPPPNLFKIIQKALEKGSLLGCSIDITSAADSEAVTYQKLVKGHAYSVTGAEEVESSG
                     SLQKLIRIRNPWGQVEWTGKWNDNCPSWNTVDPEVRANLTERQEDGEFWMSFSDFLRH
                     YSRLEICNLTPDTLTCDSYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLE
                     EEDEDEEDGERGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELTGQTNIHLGK
                     NFFLTTRARERSDTFINLREVLNRFKLPPGEYVLVPSTFEPHKDGDFCIRVFSEKKAD
                     YQAVDDEIEANIEEIDANEEDIDDGFRRLFVQLAGEDAEISAFELQTILRRVLAKRQD
                     IKSDGFSIETCKIMVDMLDEDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNS
                     YEMRKALEEAGFKLPCQLHQVIVARFADDELIIDFDNFVRCLVRLETLFKIFKQLDPE
                     NTGTIQLNLASWLSFSVL"
     exon            494..563
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            564..682
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            683..816
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            817..985
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1069
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1155
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1230
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1391
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1561
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1573
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1785
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..1822
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..1888
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..1946
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2011
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            2012..2080
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2159
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2276
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2335
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     exon            2336..3318
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3294..3299
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /note="hexamer: AATAAA"
     polyA_site      3312
                     /gene="Capn2"
                     /gene_synonym="CALP80; Capa-2; Capa2; m-calpain; m-calpin"
                     /note="major polyA site"
ORIGIN      
        1 gtagtcctca cggtcgggct ggcactgtgc aggtcgccgg cgagccgcgt ccggagcccg
       61 gcgccgagca ggcctgggga ggcggaggcc acaccccgcg cacggcccag gcgcttcccg
      121 ccggtgaatc atccccgcag cagcggctcc cgcagtccgc tgcagcgccc cgggcccggc
      181 cgcgccccag ccgagtgccg tgccccgcct ctccgcgacc cctttctccg tgcagtgcgc
      241 caaccggatc gctaccatgg cgggcatcgc gataaagctg gccaaggacc gcgaagcggc
      301 cgaggggctg gggtcgcatg agagagccat caagtacctc aaccaggact acgagacgct
      361 gcggaacgag tgcctggagg ccggggcgct cttccaggat ccttccttcc ccgccctgcc
      421 gtcatccttg ggctataagg agttggggcc ctactccagc aaaactcggg gcatcgaatg
      481 gaagcggccc acggagatct gcgctgaccc ccagttcatt attggaggag ccacccgcac
      541 agacatctgc caaggagccc tgggggactg ctggctgctg gctgccatcg cctccctcac
      601 cttgaatgag gaaatcctgg ctcgggttgt gcctccagat cagagcttcc aggaaaacta
      661 tgcaggcatc ttccacttcc agttctggca atatggcgag tgggtggagg tggtggtgga
      721 cgacaggctg cccactaagg acggggagct gctctttgtg cattcagctg aagggagtga
      781 gttctggagt gcccttctgg agaaagccta tgccaagatc aatgggtgct atgaagcact
      841 ctcagggggt gccaccacgg aaggctttga agacttcaca ggaggcattg ctgagtggta
      901 tgagctgagg aagcctcctc ccaacttgtt caagatcatc cagaaggctt tggagaaagg
      961 ttctctgctt ggctgctcta tcgatatcac cagcgctgca gattctgagg ccgttacgta
     1021 ccagaagttg gtgaaaggac atgcgtactc tgtcaccgga gccgaggagg ttgaaagttc
     1081 aggaagcctg cagaaattga tccgcatcag gaatccctgg ggacaagtag agtggaccgg
     1141 gaagtggaat gacaattgcc ccagctggaa cacggttgac ccagaagtaa gggcaaatct
     1201 aaccgaacgg caggaggacg gagaattctg gatgtccttc agtgacttcc tgagacacta
     1261 ctctcgcctg gagatctgca acctgacccc ggacaccctc acctgtgact cctataagaa
     1321 gtggaaactc accaagatgg atgggaactg gaggcgaggc tccaccgcag ggggctgcag
     1381 gaattaccca aataccttct ggatgaaccc tcagtaccta attaagctgg aggaagaaga
     1441 cgaagatgag gaggatgggg agagaggctg taccttcctg gtgggtctca tccagaagca
     1501 ccggcgacgg cagaggaaga tgggcgagga catgcacacc attggcttcg gcatctatga
     1561 ggttccagag gagctaacag ggcagaccaa catccacctc ggcaaaaact ttttcctcac
     1621 aacccgagcc agggagcggt cagatacctt cattaacctc cgcgaggtcc tcaaccgctt
     1681 caagctgccc ccgggagagt atgtcctcgt tccctccacc ttcgaacccc acaaggatgg
     1741 cgatttctgc atccgagtct tctcggagaa gaaggctgac taccaagctg ttgatgacga
     1801 gatcgaggcc aacattgaag agattgacgc caatgaggag gacattgacg atggattccg
     1861 aaggctcttt gttcagctgg ctggagagga tgcagagatc tctgcctttg agctgcagac
     1921 catcttaaga agagttctag ccaaacgcca agacatcaag tcagacggct tcagcatcga
     1981 gacctgtaag atcatggtgg acatgctgga tgaagatggg agtggcaagc ttggcctgaa
     2041 ggagttctac atcctctgga cgaagattca gaaataccaa aaaatctacc gggaaatcga
     2101 tgtggacagg tctggaacca tgaattccta cgagatgcgg aaagcactgg aagaagcagg
     2161 tttcaagctg ccctgtcaac ttcatcaagt catcgttgcc cggtttgcag acgacgagct
     2221 aatcatcgac tttgacaatt ttgtgcggtg tttggtccgt ctggaaacgc tattcaagat
     2281 attcaagcag ctagaccctg agaacactgg aacgatacag ctcaaccttg cttcgtggct
     2341 gagtttttca gtactctgaa gttacatgcc tggagaggtc tagtggaaga aaacagccaa
     2401 tgactacact tagacacttt gtatctggac cttgaactta cgggaacatt gacttcatcg
     2461 gatgattata actgaagaat ataaccgtct gggatagatg aacttttcac atatcagagt
     2521 aacatatttg catattatta aaccaaatgc aaatgaattg cataaccctt ggtaaggtca
     2581 gaggaagcgt tacacctgta aatagtatat cctttttact tctacacatt gtcctggcca
     2641 tagcaatatt aaatcaggaa ggaatgcaga gagctttaaa taactgggca aatagtaaac
     2701 caatctcaga ggctacaagg ccttgatggg aatatttgaa gaaattttaa cttttaaatg
     2761 cagtggtggc acctgccaca gcagcccaga gactgccgct tgccctgagc gaactgagaa
     2821 atacaatgac gaagtagtct gatggaggca tctaaagaac ccagtctgat ggaggcatct
     2881 aaagaatcca ggagccagga gtgtctgaca caaggccaac ctgggctgcc tagcaagacc
     2941 cgatctcaga aaataaatgc caatttaaaa aaagggcatg cttgcttcca tcatttggtt
     3001 ctaacagaag atggttttta tataccttta gcattcttaa actcaagtcc ttgaccatat
     3061 ttggccttag acacttaggt ctgacaagta gtaagtaatg agatcactct gaccaactga
     3121 gccaggtgtg ccgtctgcaa gaagatcgtg ataaacccca gggactgtgc ctacagctgg
     3181 ggaggacagg gctcagaaga tcttcttaaa acaaccaagc tggcttcttc atcctgggaa
     3241 aatgctagca accatacaga catattgcct tatctacttc caaacgtact gttaataaag
     3301 agttacaaca cagtcgcaaa aaaaaaaaaa
//
