LOCUS       NM_001025617            2957 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus calcium activated nucleotidase 1 (Cant1), transcript
            variant 2, mRNA.
ACCESSION   NM_001025617
VERSION     NM_001025617.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Niu,Y., Fan,L., Shi,X., Wu,J., Wang,T. and Hou,X.
  TITLE     Circ_0001715 accelerated lung adenocarcinoma process by the
            miR-1322/CANT1 axis
  JOURNAL   Diagn Pathol 18 (1), 91 (2023)
   PUBMED   37553672
  REMARK    GeneRIF: Circ_0001715 accelerated lung adenocarcinoma process by
            the miR-1322/CANT1 axis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2957)
  AUTHORS   Gramegna Tota,C., Valenti,B., Forlino,A., Rossi,A. and Paganini,C.
  TITLE     Phenotypic Characterization of Immortalized Chondrocytes from a
            Desbuquois Dysplasia Type 1 Mouse Model: A Tool for Studying
            Defects in Glycosaminoglycan Biosynthesis
  JOURNAL   Int J Mol Sci 22 (17), 9304 (2021)
   PUBMED   34502207
  REMARK    GeneRIF: Phenotypic Characterization of Immortalized Chondrocytes
            from a Desbuquois Dysplasia Type 1 Mouse Model: A Tool for Studying
            Defects in Glycosaminoglycan Biosynthesis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2957)
  AUTHORS   Kodama,K., Takahashi,H., Oiji,N., Nakano,K., Okamura,T., Niimi,K.,
            Takahashi,E., Guo,L., Ikegawa,S. and Furuichi,T.
  TITLE     CANT1 deficiency in a mouse model of Desbuquois dysplasia impairs
            glycosaminoglycan synthesis and chondrocyte differentiation in
            growth plate cartilage
  JOURNAL   FEBS Open Bio 10 (6), 1096-1103 (2020)
   PUBMED   32277574
  REMARK    GeneRIF: CANT1 deficiency in a mouse model of Desbuquois dysplasia
            impairs glycosaminoglycan synthesis and chondrocyte differentiation
            in growth plate cartilage.
REFERENCE   5  (bases 1 to 2957)
  AUTHORS   Paganini,C., Monti,L., Costantini,R., Besio,R., Lecci,S.,
            Biggiogera,M., Tian,K., Schwartz,J.M., Huber,C., Cormier-Daire,V.,
            Gibson,B.G., Pirog,K.A., Forlino,A. and Rossi,A.
  TITLE     Calcium activated nucleotidase 1 (CANT1) is critical for
            glycosaminoglycan biosynthesis in cartilage and endochondral
            ossification
  JOURNAL   Matrix Biol 81, 70-90 (2019)
   PUBMED   30439444
  REMARK    GeneRIF: To understanding the mechanism of CANT1 mutations in
            Desbuquois dysplasia type 1, a novel Cant1 knock-in and knock-out
            murine strains were developed. Both strains present with a skeletal
            and connective tissue phenotype recapitulating human DBQD1.
            Cartilage samples and primary chondrocytes demonstrate that CANT1
            defects not only affect GAG synthesis, but also GAG elongation,
            sulfation and PG secretion.
REFERENCE   6  (bases 1 to 2957)
  AUTHORS   Cali,T., Fedrizzi,L., Ottolini,D., Gomez-Villafuertes,R.,
            Mellstrom,B., Naranjo,J.R., Carafoli,E. and Brini,M.
  TITLE     Ca2+-activated nucleotidase 1, a novel target gene for the
            transcriptional repressor DREAM (downstream regulatory element
            antagonist modulator), is involved in protein folding and
            degradation
  JOURNAL   J Biol Chem 287 (22), 18478-18491 (2012)
   PUBMED   22451650
  REMARK    GeneRIF: Restoring CANT1 levels in neuroblastoma clones recovered
            the phenotype, thus confirming a key role of CANT1, and of the
            regulation of its gene by DREAM, in the control of protein
            synthesis and degradation
REFERENCE   7  (bases 1 to 2957)
  AUTHORS   Failer,B.U., Braun,N. and Zimmermann,H.
  TITLE     Cloning, expression, and functional characterization of a
            Ca(2+)-dependent endoplasmic reticulum nucleoside diphosphatase
  JOURNAL   J Biol Chem 277 (40), 36978-36986 (2002)
   PUBMED   12167635
REFERENCE   8  (bases 1 to 2957)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 2957)
  AUTHORS   Valenzuela,J.G., Charlab,R., Galperin,M.Y. and Ribeiro,J.M.
  TITLE     Purification, cloning, and expression of an apyrase from the bed
            bug Cimex lectularius. A new type of nucleotide-binding enzyme
  JOURNAL   J Biol Chem 273 (46), 30583-30590 (1998)
   PUBMED   9804829
REFERENCE   10 (bases 1 to 2957)
  AUTHORS   Brady,K.P., Rowe,L.B., Her,H., Stevens,T.J., Eppig,J.,
            Sussman,D.J., Sikela,J. and Beier,D.R.
  TITLE     Genetic mapping of 262 loci derived from expressed sequences in a
            murine interspecific cross using single-strand conformational
            polymorphism analysis
  JOURNAL   Genome Res 7 (11), 1085-1093 (1997)
   PUBMED   9371744
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL591075.11, CD562087.1,
            AK081118.1, AL591404.4 and BF465199.1.
            
            On Jun 6, 2012 this sequence version replaced NM_001025617.1.
            
            Summary: The protein encoded by this gene is a calcium-dependent
            nucleotidase that preferentially hydrolyzes UDP, GDP, and IDP. The
            encoded protein has low activity with ADP and ATP and shows no
            activity with AMP and GMP. Several transcript variants encoding two
            different isoforms have been found for this gene. [provided by
            RefSeq, Jun 2012].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2, 3, and 4 all encode the same isoform
            (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11927937.5184770.1,
                                           SRR10662772.3701672.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849379, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-158               AL591075.11        4755-4912           c
            159-746             CD562087.1         35-622
            747-1972            AK081118.1         804-2029
            1973-2432           AL591404.4         194203-194662       c
            2433-2957           BF465199.1         1-525               c
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 83.15 cM"
     gene            1..2957
                     /gene="Cant1"
                     /gene_synonym="5830420C20Rik; Apy1h; D11Bwg0554e; Entpd8;
                     SCAN-1; Shapy"
                     /note="calcium activated nucleotidase 1"
                     /db_xref="GeneID:76025"
                     /db_xref="MGI:MGI:1923275"
     exon            1..189
                     /gene="Cant1"
                     /gene_synonym="5830420C20Rik; Apy1h; D11Bwg0554e; Entpd8;
                     SCAN-1; Shapy"
                     /inference="alignment:Splign:2.1.0"
     exon            190..270
                     /gene="Cant1"
                     /gene_synonym="5830420C20Rik; Apy1h; D11Bwg0554e; Entpd8;
                     SCAN-1; Shapy"
                     /inference="alignment:Splign:2.1.0"
     exon            271..929
                     /gene="Cant1"
                     /gene_synonym="5830420C20Rik; Apy1h; D11Bwg0554e; Entpd8;
                     SCAN-1; Shapy"
                     /inference="alignment:Splign:2.1.0"
     CDS             293..1504
                     /gene="Cant1"
                     /gene_synonym="5830420C20Rik; Apy1h; D11Bwg0554e; Entpd8;
                     SCAN-1; Shapy"
                     /EC_number="3.6.1.6"
                     /note="isoform a is encoded by transcript variant 2;
                     apyrase 1, homolog (C. lectularius); Ca2+-dependent
                     endoplasmic reticulum nucleoside diphosphatase; soluble
                     calcium-activated nucleotidase 1; apyrase homolog"
                     /codon_start=1
                     /product="soluble calcium-activated nucleotidase 1 isoform
                     a"
                     /protein_id="NP_001020788.1"
                     /db_xref="CCDS:CCDS25702.1"
                     /db_xref="GeneID:76025"
                     /db_xref="MGI:MGI:1923275"
                     /translation="MPIQPFDQREWNEPMHSLRISVGGLPVLASMTKATDPRFRPRWR
                     VILTSFVGAALLWLLYSHHQGPVPGRPPTHNAHNWRLSQQRISHYNDTYPLSPPQRTP
                     GGIRYRIAVIADLDTGSRAQEENTWFSYLKKGYLTLSDSGDRVSVEWDKDHGVLESHL
                     AEKGRGMELSDLIVFNGKLYSVDDRTGVIYQIEGTKAVPWVILSDGDGTVEKGFKAEW
                     LAVKDEHLYVGGLGKEWTTTTGEVMNENPEWVKVVGHRGSVDHENWVSSYNALRAAAG
                     IRPPGYLIHESACWSDTLQRWFFLPRRASHERYSEKDDERKGSNLLLSAAQDFRDISV
                     RQVGTLIPTHGFSSFKFIPNTDDQIIVALKSEEDNGRIATYVMAFTLDGRFLLPETKI
                     GTVKYEGIEFI"
     exon            930..1133
                     /gene="Cant1"
                     /gene_synonym="5830420C20Rik; Apy1h; D11Bwg0554e; Entpd8;
                     SCAN-1; Shapy"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..2939
                     /gene="Cant1"
                     /gene_synonym="5830420C20Rik; Apy1h; D11Bwg0554e; Entpd8;
                     SCAN-1; Shapy"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2918..2923
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cant1"
                     /gene_synonym="5830420C20Rik; Apy1h; D11Bwg0554e; Entpd8;
                     SCAN-1; Shapy"
                     /note="hexamer: AATAAA"
     polyA_site      2939
                     /gene="Cant1"
                     /gene_synonym="5830420C20Rik; Apy1h; D11Bwg0554e; Entpd8;
                     SCAN-1; Shapy"
                     /note="major polyA site"
ORIGIN      
        1 ggcaaccgag agtgcggcgc aggaagacac ttagagcaga ggctgccggg agcgcgcggc
       61 cagagtgagg cgcaggcgca gaacagagac tgccgggagc gcgcggcggg gcggaagtgg
      121 ccgcctgccc tgtccggccc tacgcaggta ggcggcagcc cgcaggctcc ggagccaggc
      181 cctcagtggc tcccacagat acgtgcgacc ctgtcacctg agccttggaa catccttcgc
      241 agtgtggctg tcttcgccgt ggccatacag gtcctgtcca gagtgcagtc tgatgcccat
      301 ccagcccttt gaccagcggg aatggaatga gcctatgcac tccctccgga tcagtgtagg
      361 gggccttcct gtgctggcat ccatgaccaa ggccacggac cctcgcttcc gcccccgctg
      421 gcgggtgatc ctcacgtcct ttgtgggtgc tgccctcctc tggctgctgt actcccacca
      481 tcagggccca gtgccaggca ggccccccac ccacaatgcg cacaattgga ggctcagcca
      541 gcagcgcatc tctcattaca acgacaccta ccccctctcc cccccacaga ggactccagg
      601 tgggattcgg taccgaatcg cagtcattgc tgacttggac acggggtcca gggcccagga
      661 agaaaacact tggtttagct accttaagaa aggctacctg accctgtcgg acagcgggga
      721 cagggtcagc gtggagtggg ataaagacca cggggtccta gagtcccacc tggcagaaaa
      781 ggggcggggc atggagctct cagacctgat tgtcttcaat gggaaactct actccgtgga
      841 tgaccgcaca ggggtcatct accagatcga aggcaccaaa gcagtgccct gggtgatcct
      901 ttctgatggc gatggaactg tggagaaggg tttcaaagct gaatggcttg ctgtgaagga
      961 cgaacacctg tatgtgggcg gcctgggcaa ggagtggacc accacgacag gggaggtgat
     1021 gaacgagaac cctgagtggg tgaaggtggt aggccacagg ggcagtgtgg accacgagaa
     1081 ctgggtgtcc agctacaacg ccctgagagc agccgccggg atccgaccac caggctacct
     1141 cattcacgag tccgcctgct ggagcgacac actgcagcgc tggttcttcc tgcctcgtcg
     1201 ggctagccac gaacgctaca gcgagaagga cgatgaacgc aagggcagca acctcctgct
     1261 gagcgcagcc caggacttca gggacatctc tgtgaggcaa gtggggactc tgatccccac
     1321 ccacggcttc tcatccttca aattcatccc caacacggat gaccagatca tcgtggctct
     1381 caagtcggaa gaggacaatg gcagaatcgc cacctatgtc atggccttca cgctggacgg
     1441 ccgcttcctg cttccagaga ccaagatcgg cactgtgaag tacgaaggaa tagagttcat
     1501 ctagatgagt aagctcgcac gcccagaggg gcgagggcca gcggctgtgt tacaccagga
     1561 ctcataaaaa ccgggagcgc ctccactcgt ttgctttttt tcttttttta actaagagct
     1621 gtgttcctgg ctagagaatt tcattatagg gagctgagcc ccggcccata tgggtgtact
     1681 gctcggagac tgggagcaag gggtcctata caaggaaccc caggtgctcc gcccacccgc
     1741 gctcactctg cactgccact tggtggtagg gattgatcac tgctgcagct atcttctgag
     1801 cctctggcct tcagaatcca gccatgcctt tccctggtct ctggcctttc caccacttct
     1861 ctgtccagta tgtgtctttg aagcagcccc gtgttaaacc ctagcttaac cctgggagca
     1921 cacaatagtc tgaggtaggc ctgccagcca ggcactgggg gggggggggg ggggccctct
     1981 ttctcccatg tgactctggc attccatatg ctgcagaccc ctggaaagtg aatgtttaca
     2041 tggcagggtg agggccaccc cttgggaggc acctgcccac actctggagc ctctctagca
     2101 caggattgct tgcgagctgt tgataacttc atttctcccc tcaggaggat ttttgtgctg
     2161 tacagccttt taaagtccaa attattaact tatttttact agagcagttt cttttcctga
     2221 ataacagagt caccagactt catcacacag tgacagtgga gttcccacat gcacttccag
     2281 gagcgaggga cttgtcccca cacccacagt ggcgacgtga gcttttacag aaagagtctc
     2341 agcatccctg gcctctgcaa cttccaaggg ctgccatgct ttcacccctc agcagtggtt
     2401 gagggagggg ctgcacctga ctggagacct gccctcattg gctcctggtg ggtgtttagc
     2461 agccttctct ctagggaggc ctcactgttt tctgctgtcc tgttctgtcc atgttcaaaa
     2521 gagccaaggc agcagagtca ggctcagagg tcattctgca ggcagcagca cctcacctgc
     2581 ctccggcacc cttagaacag gacaccggta ctgctgtagg cacagggccc tgatgaccgt
     2641 cacaaacccc ttccacagga agccactgcc actggggcct gactaccctc agtacagcag
     2701 gctgctgctg acttgtggtg ctgaggtctt cgtgtactgt tgtgctggga gctcagccaa
     2761 acctccttga tgtgtgtcaa ctgtgttcgg ttgtgtgatt ctgagctgaa agatgaaatg
     2821 agagaggttg agagagacct gggcccctcg ctgaggagcc ttccacatac cttcctttcc
     2881 ctgagcaatg attgtgtctt tccattgttt tataaacaat aaagtgtcag gggaatgtga
     2941 aaaaaaaaaa aaaaaaa
//
