LOCUS       NM_028500               1670 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus calreticulin 3 (Calr3), transcript variant 1, mRNA.
ACCESSION   NM_028500
VERSION     NM_028500.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Fujihara,Y., Okabe,M. and Ikawa,M.
  TITLE     GPI-anchored protein complex, LY6K/TEX101, is required for sperm
            migration into the oviduct and male fertility in mice
  JOURNAL   Biol Reprod 90 (3), 60 (2014)
   PUBMED   24501175
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Tokuhiro,K., Ikawa,M., Benham,A.M. and Okabe,M.
  TITLE     Protein disulfide isomerase homolog PDILT is required for quality
            control of sperm membrane protein ADAM3 and male fertility
            [corrected]
  JOURNAL   Proc Natl Acad Sci U S A 109 (10), 3850-3855 (2012)
   PUBMED   22357757
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):5905]
REFERENCE   3  (bases 1 to 1670)
  AUTHORS   Ikawa,M., Tokuhiro,K., Yamaguchi,R., Benham,A.M., Tamura,T.,
            Wada,I., Satouh,Y., Inoue,N. and Okabe,M.
  TITLE     Calsperin is a testis-specific chaperone required for sperm
            fertility
  JOURNAL   J Biol Chem 286 (7), 5639-5646 (2011)
   PUBMED   21131354
  REMARK    GeneRIF: Calsperin is a testis-specific chaperone required for
            sperm fertility.
REFERENCE   4  (bases 1 to 1670)
  AUTHORS   Persson,S., Rosenquist,M. and Sommarin,M.
  TITLE     Identification of a novel calreticulin isoform (Crt2) in human and
            mouse
  JOURNAL   Gene 297 (1-2), 151-158 (2002)
   PUBMED   12384296
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK006582.1 and AK046081.1.
            
            On Nov 20, 2009 this sequence version replaced NM_028500.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK006582.1, AB206786.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1207              AK006582.1         2-1208
            1208-1670           AK046081.1         910-1372
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 35.08 cM"
     gene            1..1670
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /note="calreticulin 3"
                     /db_xref="GeneID:73316"
                     /db_xref="MGI:MGI:1920566"
     exon            1..186
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..1238
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; calspeculin; calsperin; calreticulin-2"
                     /codon_start=1
                     /product="calreticulin-3 isoform 1 precursor"
                     /protein_id="NP_082776.2"
                     /db_xref="CCDS:CCDS22413.1"
                     /db_xref="GeneID:73316"
                     /db_xref="MGI:MGI:1920566"
                     /translation="MVSARALLWAICVLRVALATVYFQEEFLDGERWRNRWVQSTNDS
                     QFGHFRVSSGKFYGHKEKDKGLQTTQNSRFYAISASFKPFSNKGKTLVIQYTVKHEQK
                     MDCGGGYIKVFPSDLDQKKMNGKSQYYIMFGPDICGFDIKKVHVILYFKNQYHENKKP
                     IRCKVDGFTHLYTLILRPDLSYEVKVDGQSIESGSIEYDWNLTSLRKTEKTSLDSRDW
                     DQVEGSKVQDWEKHFLDAGASKPSDWNSELDGDWLQKPPYEDGLKAEGIDKDVWLHQK
                     MRPAGYLTQYDLSEFENIGAIGLELWQVRSGTIFDNFLITDDEEYAEKFGKATWGETK
                     GPEKEMDAIQAKEEVKKAREEDEEDLLMGKFHRHNHFSRFHRQGEL"
     exon            187..288
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /inference="alignment:Splign:2.1.0"
     exon            289..492
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /inference="alignment:Splign:2.1.0"
     exon            493..587
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /inference="alignment:Splign:2.1.0"
     exon            588..776
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /inference="alignment:Splign:2.1.0"
     exon            777..872
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /inference="alignment:Splign:2.1.0"
     exon            873..1004
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1097
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1670
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1283..1288
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /note="hexamer: AATACA"
     polyA_site      1304
                     /gene="Calr3"
                     /gene_synonym="1700031L01Rik; 6330586I20Rik; Crt2; cspn"
                     /note="major polyA site"
ORIGIN      
        1 aatcccaagg catgccggga gttgtagttt ctgagttgcg cccgtcaagc ggtacggacg
       61 cctgagagca gagagttgaa gtcgcacgtg cagctatggt ctcggctcgt gcattgctct
      121 gggccatctg tgtgctgcgc gtggcactag ccactgtgta cttccaggag gagtttctag
      181 acggagagcg ctggaggaac cgctgggtgc agtccaccaa tgactcccag tttgggcact
      241 tcagagtctc atcggggaag ttctatgggc ataaagagaa agacaaaggc ctgcagacta
      301 cccaaaacag ccgattctat gccatctctg cgagcttcaa gccattcagc aacaaaggga
      361 agaccctggt aatccagtac acggtgaagc atgagcagaa gatggactgt ggtggtggtt
      421 acatcaaggt gtttccgtct gacctggacc agaagaagat gaacggaaaa tctcagtact
      481 acatcatgtt tggacctgat atttgtggat ttgatatcaa gaaagttcat gttatcttat
      541 atttcaagaa tcaatatcat gaaaacaaga agccaatcag atgcaaggtg gatggcttca
      601 cgcatctcta caccctgatc ttaagaccag atctttctta cgaggtaaaa gtcgatggcc
      661 agtccattga gtctggcagc attgagtatg actggaacct gacatcgctg aggaaaacgg
      721 aaaagacatc actggactct agggactggg atcaggtgga aggcagtaaa gtccaggatt
      781 gggaaaagca ctttctggat gcgggtgcca gcaagccgag tgactggaac agtgagctgg
      841 atggggactg gctgcagaag ccaccatatg aggatggcct gaaagctgag ggcatcgaca
      901 aggacgtgtg gctccaccag aagatgagac ctgctggcta cctgacgcag tatgacctct
      961 cagagtttga gaacattggc gccattggtc tagagctgtg gcaggtgaga tctggaacca
     1021 tctttgataa cttcctgatc acagatgatg aagaatatgc agagaagttt ggcaaggcca
     1081 cctggggaga aactaagggt ccagaaaagg agatggatgc catacaggcc aaggaagaag
     1141 tgaaaaaggc ccgtgaagaa gatgaggagg atctgctgat ggggaagttt cacaggcaca
     1201 accatttcag cagattccac aggcagggcg aactatagtc atcctcactc tgcatgctga
     1261 gtggcccagc tgcaactcta ataatacaca tttgaatgat cacatctaca tggtcatttg
     1321 tccagtttct gaaaaacaat cccctgtttg tgttagggtc ccccatttct tctatagacc
     1381 cctatgaccc cagggtctgc aataagtttc tgttgacggc atgtggtggc acactccttt
     1441 aatctcagga cttgagaggc agaagcagac agatctggga tttccacatc agcctggtcc
     1501 atgtagtgag ttctaggaca gcctgggaca catagtgaga cactgtttaa atttttttcc
     1561 ctgcatagaa cagatcattg aaagccagag acctataaga atctggccat aaacaatgtg
     1621 cttttttttt tttttgaaac tcttaaatat tttcatgaat aaaagatgat
//
