LOCUS       NM_001347427            5730 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus calcium channel, voltage-dependent, alpha 2/delta
            subunit 4 (Cacna2d4), transcript variant 2, mRNA.
ACCESSION   NM_001347427 XM_006506251
VERSION     NM_001347427.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Laird,J.G., Kopel,A., Lankford,C.K. and Baker,S.A.
  TITLE     Mouse all-cone retina models of Cav1.4 synaptopathy
  JOURNAL   Front Mol Neurosci 16, 1155955 (2023)
   PUBMED   37181655
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5730)
  AUTHORS   Klomp,A., Omichi,R., Iwasa,Y., Smith,R.J., Usachev,Y.M.,
            Russo,A.F., Narayanan,N.S. and Lee,A.
  TITLE     The voltage-gated Ca2+ channel subunit alpha2delta-4 regulates
            locomotor behavior and sensorimotor gating in mice
  JOURNAL   PLoS One 17 (3), e0263197 (2022)
   PUBMED   35353835
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5730)
  AUTHORS   Cao,Y., Wang,Y., Dunn,H.A., Orlandi,C., Shultz,N., Kamasawa,N.,
            Fitzpatrick,D., Li,W., Zeitz,C., Hauswirth,W. and Martemyanov,K.A.
  TITLE     Interplay between cell-adhesion molecules governs synaptic wiring
            of cone photoreceptors
  JOURNAL   Proc Natl Acad Sci U S A 117 (38), 23914-23924 (2020)
   PUBMED   32879010
REFERENCE   5  (bases 1 to 5730)
  AUTHORS   Collin,G.B., Gogna,N., Chang,B., Damkham,N., Pinkney,J., Hyde,L.F.,
            Stone,L., Naggert,J.K., Nishina,P.M. and Krebs,M.P.
  TITLE     Mouse Models of Inherited Retinal Degeneration with Photoreceptor
            Cell Loss
  JOURNAL   Cells 9 (4), 931 (2020)
   PUBMED   32290105
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5730)
  AUTHORS   Schlick,B., Flucher,B.E. and Obermair,G.J.
  TITLE     Voltage-activated calcium channel expression profiles in mouse
            brain and cultured hippocampal neurons
  JOURNAL   Neuroscience 167 (3), 786-798 (2010)
   PUBMED   20188150
REFERENCE   7  (bases 1 to 5730)
  AUTHORS   Shibasaki,M., Kurokawa,K. and Ohkuma,S.
  TITLE     Role of alpha2/delta subunit in the development of morphine-induced
            rewarding effect and behavioral sensitization
  JOURNAL   Neuroscience 163 (3), 731-734 (2009)
   PUBMED   19596051
REFERENCE   8  (bases 1 to 5730)
  AUTHORS   Wycisk,K.A., Budde,B., Feil,S., Skosyrski,S., Buzzi,F.,
            Neidhardt,J., Glaus,E., Nurnberg,P., Ruether,K. and Berger,W.
  TITLE     Structural and functional abnormalities of retinal ribbon synapses
            due to Cacna2d4 mutation
  JOURNAL   Invest Ophthalmol Vis Sci 47 (8), 3523-3530 (2006)
   PUBMED   16877424
  REMARK    GeneRIF: The Cacna2d4 mutation underlies a novel channelopathy
            leading to cone-rod dysfunction in the visual system of mice
REFERENCE   9  (bases 1 to 5730)
  AUTHORS   Obermair,G.J., Kugler,G., Baumgartner,S., Tuluc,P., Grabner,M. and
            Flucher,B.E.
  TITLE     The Ca2+ channel alpha2delta-1 subunit determines Ca2+ current
            kinetics in skeletal muscle but not targeting of alpha1S or
            excitation-contraction coupling
  JOURNAL   J Biol Chem 280 (3), 2229-2237 (2005)
   PUBMED   15536090
REFERENCE   10 (bases 1 to 5730)
  AUTHORS   Ruether,K., Grosse,J., Matthiessen,E., Hoffmann,K. and Hartmann,C.
  TITLE     Abnormalities of the photoreceptor-bipolar cell synapse in a
            substrain of C57BL/10 mice
  JOURNAL   Invest Ophthalmol Vis Sci 41 (12), 4039-4047 (2000)
   PUBMED   11053310
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC115816.9.
            
            On May 2, 2019 this sequence version replaced NM_001347427.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' coding region compared to variant 1. It encodes
            isoform 2 which is shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC141091.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164136 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-346               AC115816.9         77497-77842
            347-428             AC115816.9         79995-80076
            429-545             AC115816.9         81638-81754
            546-605             AC115816.9         82126-82185
            606-768             AC115816.9         83751-83913
            769-900             AC115816.9         85172-85303
            901-961             AC115816.9         108780-108840
            962-1112            AC115816.9         109169-109319
            1113-1187           AC115816.9         111727-111801
            1188-1277           AC115816.9         112189-112278
            1278-1391           AC115816.9         112382-112495
            1392-1470           AC115816.9         112846-112924
            1471-1604           AC115816.9         115198-115331
            1605-1682           AC115816.9         118035-118112
            1683-1763           AC115816.9         118793-118873
            1764-1850           AC115816.9         122593-122679
            1851-1922           AC115816.9         123152-123223
            1923-1984           AC115816.9         138451-138512
            1985-2052           AC115816.9         139403-139470
            2053-2098           AC115816.9         141182-141227
            2099-2196           AC115816.9         141301-141398
            2197-2290           AC115816.9         142999-143092
            2291-2386           AC115816.9         148219-148314
            2387-2514           AC115816.9         149650-149777
            2515-2595           AC115816.9         153396-153476
            2596-2657           AC115816.9         177364-177425
            2658-2702           AC115816.9         178855-178899
            2703-2765           AC115816.9         179088-179150
            2766-2836           AC115816.9         184503-184573
            2837-2912           AC115816.9         184940-185015
            2913-2965           AC115816.9         185501-185553
            2966-3018           AC115816.9         186017-186069
            3019-3039           AC115816.9         186403-186423
            3040-3157           AC115816.9         188107-188224
            3158-3270           AC115816.9         189699-189811
            3271-3353           AC115816.9         190068-190150
            3354-5730           AC115816.9         191004-193380
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 56.62 cM"
     gene            1..5730
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /note="calcium channel, voltage-dependent, alpha 2/delta
                     subunit 4"
                     /db_xref="GeneID:319734"
                     /db_xref="MGI:MGI:2442632"
     exon            1..346
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..3458
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; voltage-dependent calcium channel subunit
                     alpha-2/delta-4; voltage-gated calcium channel subunit
                     alpha-2/delta-4"
                     /codon_start=1
                     /product="voltage-dependent calcium channel subunit
                     alpha-2/delta-4 isoform 2 precursor"
                     /protein_id="NP_001334356.2"
                     /db_xref="CCDS:CCDS85143.2"
                     /db_xref="GeneID:319734"
                     /db_xref="MGI:MGI:2442632"
                     /translation="MARCPMLSSSHNQDHSGRWTAVWTSLWKTPIILWLLLSDTSLPT
                     VRGQTTVPLETVKLWADTFGRNLYNTVTRYSGSLLLQKKYKDAEPSLKIKEVDGLELV
                     KKFSEDMETMLRRKVEAVESLVEAAEEADLNHEFNASLVFNYYNSVLINEKDDKGNYV
                     ELGAEFLLESDAHFSNLRVNVSMSSVQLPTNVYNKDPDILNGVYMSEALNPVFVENFQ
                     RDPTLTWQYFGSSTGFFRIYPGIKWMPDENGVIAFDCRNRGWYIQAATSPKDIVILVD
                     ISGSMKGLRMAIAKHTITTILDTLGENDFVNIIAYNDYVHYIEPCFKGILVQADRDNR
                     EHFKQLVDELMVKGVGVVSQALIEAFEILKQFQESKQGSLCNQAIMLITDGAVEDYEP
                     VFETYNWPDRKVRVFTYLIGREVTFADRMKWIACNNKGYYTQISTLADAQESVMEYLH
                     VLSRPMVINHDHDIIWTEAYMDSRLFTSEAQSLMLLTTVAMPVFSKKNETRSHGILLG
                     VVGSDVTLRELMKLAPRYKYREGKKLRPKPNYNSVDLSEVEWEDQAEILRTAMINGET
                     GSHSMDVKVPLDKGKRVLFLTNDYFFTDISDTPFSLGVVLTRGHGEYILLGNTSVEEG
                     LHDLLHPDLTLASDWIYCITDIDPDHRKLSQLEAVVRFLTGVDPDLECDEELVREVLF
                     DAVVTAPMEAYWTALALNISEESEPGVDVAFLGTRAGLLRRSLFVGSEKVSDRKFLTP
                     EDEASIFTMDHFPLWYRQASEQPPGSFVFNLRWAEGPDSPGKPVAVRASTAVTVTVDG
                     KTAIAAAVGIQMQADYLQRQFWAAMQQCNAVEGPCLKSCEDTDLDCFVIDNNGFVLIS
                     ERPQEMGRLLGEADGALMKQLLSMGVFSRVTMYDYQAMCKPPDHHHSAAESLFSPLSA
                     FLMVARWLLHECLLFLLEWSAWGSWQDKGSEAKSVFHHSHKHKKQDLLHPCDTEYPVF
                     VHQTAIQEANGIIECGGCQKTFVMQQIPRSNLLLLVTDRTCDCSAHSPILQEATEVKY
                     NASVKCDRMRSQKPRRRPGSCHAFHPEENAQDCGGASDTLPSSPLLLLSLGAWLLPPQ
                     LLW"
     exon            347..428
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            429..545
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            546..605
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            606..768
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            769..900
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            901..961
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1112
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1187
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1277
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1391
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1470
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1604
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1682
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1763
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1850
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..1922
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..1984
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2052
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2098
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2099..2196
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2197..2290
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2291..2386
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2387..2514
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2515..2595
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2596..2657
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2658..2702
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2703..2765
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2766..2836
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2837..2912
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2913..2965
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2966..3018
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3019..3039
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3040..3157
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3158..3270
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3271..3353
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3354..5730
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4847..4852
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4875
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /note="major polyA site"
     regulatory      5711..5716
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5730
                     /gene="Cacna2d4"
                     /gene_synonym="5730412N02Rik"
                     /note="major polyA site"
ORIGIN      
        1 actgccttcc cctttgatca cctggggtgg accctctgcc cgctgcagct gggggcaaca
       61 tcttttactt gggactggac agcaggcaga acacttgcat gcccaggagt tcctgtggca
      121 gcaagttatc tcccagcagg ggctctgtcc tcctcccctg cccctccccc agtgggaaca
      181 ccatggcaag atgccccatg ctgtcctcta gccacaacca agaccacagt ggacggtgga
      241 cagcagtgtg gacctccctg tggaagacgc caatcatcct gtggctgctg ctctcggaca
      301 cctccctgcc taccgtgagg ggccagacca cagttcctct ggaaacagtg aagctgtggg
      361 ccgatacctt cggcaggaat ctgtacaaca cggtgaccag atattcaggc tccctcctgc
      421 tgcagaagaa gtacaaggat gcagaaccca gtctgaagat caaagaggtg gatggcttgg
      481 agctggtgaa gaagttctcg gaggacatgg agaccatgct tcggaggaaa gtcgaggctg
      541 ttgagagcct ggtggaggcc gctgaggagg ctgacctgaa tcatgagttc aatgcatctc
      601 tggtgtttaa ctactacaac tcggtactaa tcaatgagaa ggatgacaag ggcaactacg
      661 tggagctggg agctgagttt cttctcgagt ccgacgcgca cttcagcaac ctgagggtga
      721 atgtctccat gagcagcgtt caactgccca ccaacgtgta caacaaagac ccagacattt
      781 taaatggcgt gtatatgtcc gaagccctga accccgtgtt tgtggagaac tttcagagag
      841 accctacatt gacatggcag tatttcggca gctcaactgg gttcttcagg atctatccag
      901 gaataaagtg gatgcctgat gaaaacggag tcatcgcctt cgactgcaga aaccgtggct
      961 ggtacataca agctgccaca tctcccaaag acatcgtaat tctggtggac attagcggca
     1021 gcatgaaggg gctgaggatg gctatcgcca agcacaccat taccaccata ttggacaccc
     1081 tgggagagaa cgacttcgtg aacatcatcg cgtacaatga ctacgtccac tacattgagc
     1141 cctgcttcaa aggcatcctt gtccaagcag atcgagacaa ccgagagcat ttcaagcagt
     1201 tggtggatga gctgatggtc aaaggtgtgg gtgtcgtgag tcaagctcta attgaagctt
     1261 tcgagatcct gaaacagttc caagagtcca aacaaggaag tctctgcaac caggccatca
     1321 tgcttatcac cgatggggct gtggaagact atgagcccgt gtttgagacc tataactggc
     1381 cagaccgaaa ggtccgagtt ttcacttacc ttattggaag agaagtgact ttcgctgacc
     1441 gcatgaagtg gatcgcctgc aacaataaag gctactacac acagatctcc acactggcgg
     1501 atgcgcagga gagcgtgatg gagtacctgc atgtgcttag ccgccccatg gtcatcaacc
     1561 atgaccacga catcatctgg acagaggctt acatggacag ccggctcttc acatcagagg
     1621 cacagagcct gatgctcctc accacagtgg ccatgcctgt cttcagcaaa aagaatgaaa
     1681 caagatccca tggcattctc ctgggtgtag tgggctctga cgtgacccta agagagctca
     1741 tgaagctggc accccgatat aagtacagag aaggcaagaa gctgagaccc aaacccaact
     1801 acaacagtgt ggacctctca gaagtggagt gggaggacca agctgaaatt ctgaggaccg
     1861 ccatgatcaa tggggaaacc gggtctcact ccatggacgt gaaggtgcca ctggacaaag
     1921 ggaagcgagt tctattcctg accaatgact acttcttcac agacatcagt gacacacctt
     1981 tcagcctggg agtggtgctc accagaggtc atggagaata catcctcctg gggaacacat
     2041 ctgtggaaga aggcctgcac gacttgcttc atccggatct gaccctggcc agtgactgga
     2101 tctactgtat cacagatatc gacccggacc accggaaact cagccaactg gaagctgtgg
     2161 ttcgtttcct gacaggggtg gatccagacc tggagtgtga cgaggaactg gtgcgggagg
     2221 tgctgtttga tgcggtggta accgccccca tggaagccta ttggacagca ctggcactca
     2281 acatctctga agagtcagag cctggcgtgg atgtggcctt cctgggaacc cgggctggcc
     2341 ttctaagaag aagcttgttc gtaggctccg agaaagtctc tgacaggaag ttcctgaccc
     2401 ctgaagatga agccagtatt tttaccatgg accacttccc gctgtggtat cgccaggcct
     2461 ctgagcagcc ccccggcagc tttgtcttca atctccgctg ggcagaggga ccagatagcc
     2521 caggcaagcc agtggcggta agggccagca cagcagtaac tgtgaccgtg gatgggaaga
     2581 cggccattgc agcagctgtg ggcatccaga tgcaagcaga ctacctccag cgccagttct
     2641 gggcagccat gcagcagtgc aacgctgtag aggggccgtg cctgaagagc tgcgaggaca
     2701 ctgatctcga ctgcttcgtc atagacaaca atggcttcgt cctgatctca gagagacccc
     2761 aagagatggg aagacttctg ggggaagcgg atggtgctct catgaagcaa cttctcagca
     2821 tgggggtgtt cagccgtgtg accatgtatg actaccaggc catgtgcaag cccccagatc
     2881 atcaccacag tgcggccgaa tccctgttca gtcctctctc tgccttcttg atggtggcca
     2941 ggtggctact gcatgaatgt ctcctattcc tgttagagtg gagtgcctgg ggatcttggc
     3001 aggacaaagg gtcagaggcc aaatctgtct tccatcactc ccacaagcac aagaagcagg
     3061 acctgctgca cccctgtgac acagagtacc cagtgttcgt gcatcagacg gccatccagg
     3121 aagccaatgg catcatcgag tgtgggggct gccagaagac attcgtgatg caacagattc
     3181 cccgaagcaa cctgctcctg ctcgtgacag accgcacctg tgactgcagc gcccactctc
     3241 ccatcctcca ggaggccaca gaagtcaaat ataatgcctc tgtcaagtgt gacaggatgc
     3301 gctcccagaa gccccggaga cgaccaggct cctgccatgc cttccatcca gaggagaatg
     3361 cccaggactg tggcggtgct tcagacactc tgccttcatc ccctctgctc ctgctatcac
     3421 tgggggcctg gctgcttcca ccccagcttc tgtggtgact ccatcccatc tgactttcgc
     3481 agaaggggga ccttacagga cattctacaa agttaccggc ttatgaaggc ccctcactgc
     3541 tgtgggatgt ctcctgtttc agtcctgggg ctacaagtct ctgcttctta gagattgcta
     3601 gagggatctc caaggctctt caagtcttct actcttgtct caagtgaagg aaggacagaa
     3661 gttcattact ccagtttacc aaagggtcac ccatttccct ggctgatctg ctgatccgag
     3721 gagttctgga ggctggaatt gagcggcgag gcccatgtag aggctgtggc aagagtgtgc
     3781 ttgccaccaa agggaaggtg cttttatcca tcagatagtg cagaggacag gcagagggag
     3841 agacaaaaga ctagggaagg gaccagaaga ggagggaaga aatggaaagg aagctgggcg
     3901 tggtggttca cactttcaat cccagcacaa acggaaacaa taggaacgca atggatatta
     3961 agtcctgcga gctgtcatta gctacctctg aagagtctca acctttttac actaatgatc
     4021 tggagagcgg gcaggggtgt atgggaggag gtggagggga agggaaggga taaatgctgt
     4081 aattataatc tcagaaaggg aaagaaaagc ttttaggatg agctctgcag ctgctgcact
     4141 gggccaagca ccagtgtggc actggacagg catgatcctg aggaggtggc gagaaaaagc
     4201 agaaccgaga ctcgcgcctc acagactaaa ggaaggagcc aggtgaggtc tccaagagcc
     4261 atagtgggct ttctgtggct cacaatgtac aaaagaaacc agactgttgc tggggaaagt
     4321 cctggaggct ggaaacaaca cataatcatt gccacaatgc ccaagtgaac acacacacac
     4381 gcccgtgcgc acacatttgt gtacctgttt gtacaaagat gtggaggcca aaggtaatat
     4441 tggccgtttt cctcaattac tcctttaatt ttttttttag ataagatctc tcacaaaatc
     4501 tgagtcctcc aattcatagc tggccagcaa gccccagaag cccttctgtc actgtctccc
     4561 caaggctggg actgcaagca tgccccgctg tagccctact gttggaatag ttcaggaacc
     4621 taacttgggc cctcattctc cactagctga acctctcaag ccccgcgtat ctatctttta
     4681 aatcaggaac ctaaactggc agccgtggtg cacgccttga atcccaggac tgggaggcag
     4741 agggaggaag atctctaagt atgaggctag cctggtctat aaaatgacag acacccaggg
     4801 ctacacagaa accttatctt gaagataaaa aaataaatag aaaaagaata aaagctaaaa
     4861 cttggagaag ggaaactggt tctaagtctc ggctttgtgt agtaggaggc aatgtaccta
     4921 gcaccagcta gcactcagta agcactaaca gcacactagc tagcatcctg catcggggat
     4981 tggaacgtag ggaaggtcag aggtgctgcc aggtgaagta ccaaactgtt atagcagtgt
     5041 tttctctgtc aggcacggca ggtctttcct gaccaccgtc acatcacctg ccttgtcatc
     5101 agtgttatga cctcctttca gcagcgtcag gtctcaatgg caagcaggca agtctcttaa
     5161 aggacttgtc tccttcaccc aaccaggcag aacatttggg gacgttgatc aactctttag
     5221 gccactggag aaatgctaaa tgtagggtgc caggcagctc ctggtcagga gccacggggc
     5281 tctcgtgcct ggcactgagg aatcgtttag aacaccacca gagcagctga agagaaggca
     5341 tttcatgatg ctctgtccct accatggaca ccaggtatct cccaaggtgt gcctgcctct
     5401 caattctgag actagtgccg ccaaaggaca tgtaggagga tggccaagac atgacgtggc
     5461 aagctaagcc ctcctcagtg agtgatcata actccaccaa gacactggaa gactgcagtc
     5521 tggattaggt gaaagatctg gcaccttgac tttagaggaa tgaagttggc acccccgaga
     5581 ttaaatgaat ggaatgcagc attctggcat ctttccttat ctgtgttgcc ttcaaccaca
     5641 agagctggag ccctgtcaga agcaccagga atgtgccgct gagcttatgt gctagagtag
     5701 aaccacctca aataaaaggc ttcaacagca
//
