LOCUS       NM_001110844            7436 bp    mRNA    linear   ROD 21-AUG-2024
DEFINITION  Mus musculus calcium channel, voltage-dependent, alpha2/delta
            subunit 1 (Cacna2d1), transcript variant b, mRNA.
ACCESSION   NM_001110844
VERSION     NM_001110844.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Roy,D., Dion,E., Sepeda,J.A., Peng,J., Lingam,S.R., Townsend,K.,
            Sas,A., Sun,W. and Tedeschi,A.
  TITLE     alpha2delta1-mediated maladaptive sensory plasticity disrupts
            adipose tissue homeostasis following spinal cord injury
  JOURNAL   Cell Rep Med 5 (5), 101525 (2024)
   PUBMED   38663398
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Lankford,L., Maddala,R., Jablonski,M.M. and Rao,P.V.
  TITLE     Influence of the calcium voltage-gated channel auxiliary subunit
            (CACNA2D1) absence on intraocular pressure in mice
  JOURNAL   Exp Eye Res 241, 109835 (2024)
   PUBMED   38373629
  REMARK    GeneRIF: Influence of the calcium voltage-gated channel auxiliary
            subunit (CACNA2D1) absence on intraocular pressure in mice.
REFERENCE   3  (bases 1 to 7436)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 7436)
  AUTHORS   Dolphin,A.C.
  TITLE     Calcium channel auxiliary alpha2delta and beta subunits:
            trafficking and one step beyond
  JOURNAL   Nat Rev Neurosci 13 (8), 542-555 (2012)
   PUBMED   22805911
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7436)
  AUTHORS   Li,C.Y., Zhang,X.L., Matthews,E.A., Li,K.W., Kurwa,A.,
            Boroujerdi,A., Gross,J., Gold,M.S., Dickenson,A.H., Feng,G. and
            Luo,Z.D.
  TITLE     Calcium channel alpha2delta1 subunit mediates spinal
            hyperexcitability in pain modulation
  JOURNAL   Pain 125 (1-2), 20-34 (2006)
   PUBMED   16764990
REFERENCE   6  (bases 1 to 7436)
  AUTHORS   Bian,F., Li,Z., Offord,J., Davis,M.D., McCormick,J., Taylor,C.P.
            and Walker,L.C.
  TITLE     Calcium channel alpha2-delta type 1 subunit is the major binding
            protein for pregabalin in neocortex, hippocampus, amygdala, and
            spinal cord: an ex vivo autoradiographic study in alpha2-delta type
            1 genetically modified mice
  JOURNAL   Brain Res 1075 (1), 68-80 (2006)
   PUBMED   16460711
  REMARK    GeneRIF: alpha(2)-delta Type 1 subunit of voltage-gated calcium
            channels is the major binding protein for pregabalin in the brain
            and spinal cord.
REFERENCE   7  (bases 1 to 7436)
  AUTHORS   Obermair,G.J., Kugler,G., Baumgartner,S., Tuluc,P., Grabner,M. and
            Flucher,B.E.
  TITLE     The Ca2+ channel alpha2delta-1 subunit determines Ca2+ current
            kinetics in skeletal muscle but not targeting of alpha1S or
            excitation-contraction coupling
  JOURNAL   J Biol Chem 280 (3), 2229-2237 (2005)
   PUBMED   15536090
REFERENCE   8  (bases 1 to 7436)
  AUTHORS   Angelotti,T. and Hofmann,F.
  TITLE     Tissue-specific expression of splice variants of the mouse
            voltage-gated calcium channel alpha2/delta subunit
  JOURNAL   FEBS Lett 397 (2-3), 331-337 (1996)
   PUBMED   8955374
REFERENCE   9  (bases 1 to 7436)
  AUTHORS   Chin,H., Mock,B., Kim,H.L., Kim,H. and Kozak,C.A.
  TITLE     The gene for the dihydropyridine-sensitive calcium channel alpha 2
            subunit (CCHL2A) maps to the proximal region of mouse chromosome 5
  JOURNAL   Genomics 13 (4), 1325-1327 (1992)
   PUBMED   1324224
REFERENCE   10 (bases 1 to 7436)
  AUTHORS   Jay,S.D., Sharp,A.H., Kahl,S.D., Vedvick,T.S., Harpold,M.M. and
            Campbell,K.P.
  TITLE     Structural characterization of the dihydropyridine-sensitive
            calcium channel alpha 2-subunit and the associated delta peptides
  JOURNAL   J Biol Chem 266 (5), 3287-3293 (1991)
   PUBMED   1847144
  REMARK    GeneRIF: This study of the rabbit ortholog of
            dihydropyridine-sensitive calcium channel alpha 2 provides
            N-terminal sequencing evidence of the cleavage junction of the
            alpha2 and delta subunits from the preprotein
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK164580.1, BC115871.1 and
            AC158586.8.
            
            Summary: This gene encodes a regulatory component of the
            voltage-dependent calcium channel complex. The product of this gene
            is a proprotein that is proteolytically processed into alpha-2 and
            delta subunits, which are linked by a disulfide bond. Alternatively
            spliced transcript variants encoding multiple isoforms have been
            observed for this gene. [provided by RefSeq, Jan 2013].
            
            Transcript Variant: This variant (b) lacks an exon and contains an
            alternate exon in the coding region, but maintains the reading
            frame, compared to variant a. The encoded isoform (b), is shorter
            than isoform a, and is processed into alpha-2b and delta-1
            subunits.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U73484.1, MK327276.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849390, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-249               AK164580.1         2-250
            250-1913            BC115871.1         25-1688
            1914-1928           AC158586.8         169364-169378       c
            1929-3652           BC115871.1         1689-3412
            3653-7436           AC158586.8         121179-124962       c
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 6.56 cM"
     gene            1..7436
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /note="calcium channel, voltage-dependent, alpha2/delta
                     subunit 1"
                     /db_xref="GeneID:12293"
                     /db_xref="MGI:MGI:88295"
     exon            1..418
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             324..3599
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /note="isoform b preproprotein is encoded by transcript
                     variant b; voltage-dependent calcium channel subunit
                     alpha-2/delta-1; voltage-gated calcium channel subunit
                     alpha-2/delta-1; dihydropyridine-sensitive calcium channel
                     alpha 2; voltage-dependent calcium channel alpha2/delta-1"
                     /codon_start=1
                     /product="voltage-dependent calcium channel subunit
                     alpha-2/delta-1 isoform b preproprotein"
                     /protein_id="NP_001104314.1"
                     /db_xref="CCDS:CCDS51422.1"
                     /db_xref="GeneID:12293"
                     /db_xref="MGI:MGI:88295"
                     /translation="MAAGCLLALTLTLFQSGLIGPSSEEPFPSPVTIKSWVDKMQEDL
                     VTLAKTASGVTQLADIYEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLAM
                     EAEKVQAAHQWREDFASNEVVYYNAKDDLDPERNESEPGSQRIKPVFIEDANFGRQIS
                     YQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKRNRDEDPTLLWQVFGSATGLARYY
                     PASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSV
                     SEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYK
                     KGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQH
                     NYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVY
                     LDALELGLVITGTLPVFNVTGQSENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCP
                     NGYYFAIDPNGYVLLHPNLQPKNPKSQEPVTLDFLDAELENEIKVEIRNKMIDGESGE
                     KTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQAR
                     SKKGKMKDSETLKPDNFEESGYTFIAPREYCNDLKPSDNNTEFLLNFNEFIDRKTPNN
                     PSCNTDLINRILLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENW
                     QENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVELYIQGKLLKPA
                     VVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYT
                     NQIGRFFGEIDPSMMRHLVNISLYAFNKSYDYQSVCDPGAAPKQGAGHRSAYVPSIAD
                     ILQIGWWATAAAWSILQQLLLSLTFPRLLEAVEMEEDDFTASLSKQSCITEQTQYFFK
                     NDTKSFSGLLDCGNCSRIFHVEKLMNTNLVFIMVESKGTCPCDTRLLMQAEQTSDGPD
                     PCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPSLWSIFGLQFILLWLVSGSRH
                     YLL"
     exon            419..500
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            501..617
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            618..677
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            678..719
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            720..849
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            850..981
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1051
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1102
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1202
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1361
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1466
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1545
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1595
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1596..1685
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..1763
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1838
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..1913
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1914..1985
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2057
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2058..2119
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2120..2196
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2197..2217
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..2278
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..2376
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2464
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2465..2527
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2528..2631
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2632..2718
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2719..2786
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2787..2825
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2826..2897
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2898..3050
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3051..3103
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3104..3159
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3160..3289
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3290..3399
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3400..3482
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3483..7436
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7406..7411
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /note="hexamer: AATAAA"
     polyA_site      7430
                     /gene="Cacna2d1"
                     /gene_synonym="Ca(v)alpha2delta1; Cacna2; Cchl2a"
                     /note="major polyA site"
ORIGIN      
        1 ctctcctcgg cgaccgcgga taaatcaggg aggcgagcgc tgggagccag agcgcgcggg
       61 cgccggcggg ctggccgagc tctgtttcca cagtcgccct tgatggcggc gcaggcgagg
      121 cagccgtggc acggagcggc agccacgcgc tagagagtcc gcccggcccc gcccggcccg
      181 cccggccgcc cgcccgtgcc cagcccgctt ctccccgcgc gctctcccgc agacgtcggc
      241 gccccggctc cgtccctcag ccgcctcgtg cagctccgcc gcctccgggc cggcagggag
      301 ggcactgatc ttcgatcgcg aagatggctg ctggctgcct gctggccttg actctgacac
      361 ttttccaatc tgggctgatc ggcccctcga gcgaggagcc cttcccttcg cccgtcacta
      421 tcaagtcatg ggtggacaag atgcaagaag accttgtcac actggcaaaa acagcaagtg
      481 gagtgactca gcttgctgat atttatgaaa aataccaaga tttgtatact gtggagccca
      541 acaatgcacg ccaactggtt gaaattgcag cccgagacat tgagaagctt ctaagcaaca
      601 gatctaaggc cctggtgcgc ctggctatgg aagcagagaa agtccaagca gcccaccaat
      661 ggagggaaga ttttgcaagc aatgaagttg tctactataa tgctaaggat gatcttgatc
      721 ctgaaagaaa tgagagtgag ccaggcagcc aacggattaa acctgttttt attgaagatg
      781 ctaattttgg acgtcagata tcctatcagc atgcagcggt ccatattccc acggacatct
      841 atgagggctc aaccatagtg ttaaacgaac tcaactggac aagtgcctta gatgaagtat
      901 tcaaaagaaa tcgagacgaa gaccctacac tgctgtggca agtgtttggc agcgccactg
      961 gcctggcccg atattatcca gcttctccat gggtggataa tagtagaact ccaaacaaga
     1021 ttgatctata tgatgtacgc agaagaccat ggtacatcca gggagctgca tcccccaagg
     1081 acatgctcat tctggtggac gtgagtggaa gtgtgagcgg attgactctg aaactcatcc
     1141 gaacatctgt ctccgagatg ttagaaaccc tctctgatga tgatttcgtg aatgtagctt
     1201 catttaacag caacgctcag gatgtaagct gtttccagca cctggttcaa gcgaatgtaa
     1261 gaaataagaa ggtgttgaaa gatgccgtga ataacattac agcaaagggg atcacagatt
     1321 acaagaaagg ctttagcttt gccttcgaac agctacttaa ttataatgtt tccagagcta
     1381 attgcaataa gattatcatg ttattcacgg atggaggaga agagagagcc caggagatat
     1441 ttgccaaata caataaagac aaaaaagtcc gtgtgtttac attttccgtc ggtcaacata
     1501 attatgacag aggacctatt cagtggatgg cttgtgaaaa taaaggttac tattatgaga
     1561 ttccctccat tggtgcaata agaatcaata ctcaggaata tctagatgtt ctgggaagac
     1621 caatggtttt agctggtgac aaagcgaagc aagttcaatg gacaaatgtg tatttggacg
     1681 ccctggaact gggacttgtc attactggaa ctctaccagt cttcaacgtc actggccaat
     1741 ctgaaaataa gacaaacttg aagaaccagt tgattcttgg tgtgatgggc gttgatgtgt
     1801 ctctggaaga tatcaagaga ttgacaccac gttttacact ctgtcccaat ggctactatt
     1861 ttgcaattga tcctaatggc tatgtcttat tgcatccaaa tcttcagcca aagaacccca
     1921 aatctcagga gccagtcaca ctggattttc tcgatgctga gttagagaat gaaattaaag
     1981 tggagattcg aaataaaatg atagatggag aaagtggaga aaaaacgttc agaactctgg
     2041 tcaagtctca agatgagaga tacattgaca aaggaaatcg aacatacaca tggacgcctg
     2101 tcaatggcac agattacagt ttggccttgg tattgccaac ctacagtttt tactatataa
     2161 aagccaaact agaagagaca ataactcagg ccagatcaaa aaagggaaaa atgaaggatt
     2221 cagaaaccct gaagccagac aattttgaag aatctggcta tactttcata gcaccaaggg
     2281 aatactgcaa tgatcttaaa ccttcagata ataacactga atttcttttg aatttcaatg
     2341 aatttattga taggaaaact ccaaacaacc cctcctgtaa tacagatttg attaatagaa
     2401 tcttgctgga tgcaggtttt acaaatgaac ttgtccaaaa ttattggagt aagcagaaaa
     2461 atatcaaagg agtgaaggca cgctttgtgg tgacagacgg tgggattacg agagtttatc
     2521 ccaaagaggc cggagaaaat tggcaagaga acccagagac gtacgaggac agcttctaca
     2581 aacggagcct agataacgat aactacgttt tcactgcgcc ctactttaac aaaagtggac
     2641 ctggtgcgta tgaatctgga attatggtaa gcaaagcagt agaactgtat atccaaggaa
     2701 aacttcttaa gcccgcagtt gtgggaatta aaattgatgt aaattcttgg atagaaaatt
     2761 ttaccaaaac ttcaatcagg gatccgtgtg ctggtccagt ttgtgactgc aaaagaaaca
     2821 gtgatgtaat ggactgtgtc attctagatg atggtggatt tcttttgatg gcaaatcacg
     2881 atgattacac taatcagatt ggacgttttt ttggagagat tgacccaagc atgatgagac
     2941 acctggttaa tatatcactt tatgcattca acaaatcata tgactatcag tccgtgtgcg
     3001 atccaggggc agcaccaaag cagggggcag gacatcgctc agcatatgtg ccatcgattg
     3061 cagatatact gcagattggc tggtgggcca ccgctgccgc ctggtctatt ctccagcagc
     3121 tgctcttgag tttgacattt ccacggctcc ttgaggcagt tgagatggag gaagatgact
     3181 tcacagcctc cctgtctaag cagagctgca tcacagaaca aacccagtac ttcttcaaga
     3241 acgatactaa atcattcagt ggtttactgg actgtggaaa ctgttccagg atctttcatg
     3301 tagagaaact tatgaacacc aacttagtat tcataatggt ggagagcaaa gggacatgtc
     3361 cgtgtgacac gcggctgctc atgcaagcgg aacagacttc tgatggtcca gatccttgcg
     3421 acatggtcaa gcagcccaga taccgaaaag gacctgatgt ctgctttgat aataatgtgc
     3481 tggaggatta tactgactgt ggtggtgttt ctgggttaaa cccttcctta tggtctatct
     3541 ttggactcca gtttatactc ctttggctgg tatctggcag cagacactac ctactgtgac
     3601 cttctagaac ccagatctgc aacgaaattc caagccctgc caaaacatga gccctcagtt
     3661 acattaaata ggagtcggtt gaagaagcga cacagaactt tagctgggcc tctaccatga
     3721 cgtaactaag ccttagctta gattcttgac catcgctggc tgcatgtcag ggtgttggaa
     3781 actgaaactt gtgtgaatat gctgcatcat ctatgtctaa catcaaagca aaactctact
     3841 tgttctctgt gggaggtttg ggagttgact gttgcacggt tggagagtac gtgtaaaagg
     3901 ctcccccacg caggccttat gaaaagaagc ggtcttgact ttgacctagc atttcaacct
     3961 gctgcacttt taccaagaag aagaaaaaag aaaaccctag tgagggccaa tgatattttt
     4021 gccttcactt atttgctgtt caaaaataat tcctgctgtg agtaattatt aaccagagag
     4081 ggagagaggg agggagaaaa tacattggtt taaactgatg aaataataat aaaaaaaaaa
     4141 ctaatggcta attttctacc aaaaaaaaat tgccatgaat aaaatgcctt ataaaagaat
     4201 gacttctctg ccaaaaacaa aacacaactg tgaccagttg aatttggata aggtctgaag
     4261 tgctggtttg aaaccaagga cagaataagc taaaggtgct tgtgggtgaa attggaattc
     4321 atgatacatt tcctcataaa tgtaagccct taactgcgtg atgtaatgtc ccttttgttc
     4381 acagtaaagt catgtagtgc tagccagata tttaacatgc tctaagagaa gcttgttggg
     4441 gggaggggaa aggttttcct tgtggttaat gaacttggct tgccaagggt cttttgcatg
     4501 atgctgctgc tattttaact ttctggtttg tttttcttct tactgtagag tatagtccat
     4561 tgacaagtta actgggtgac attattgtca tgtaagaatc tgaaccttgc agtgtacatg
     4621 cacctaagtc atccttaaca tgttgttaaa ctactctgaa tatactgtgc aatgtaaatg
     4681 tcgaacggct gggttaggca gacggcgaag tgagaattca tggattttat acagactcat
     4741 gcttttgcct gaaaaaccta tgtacagata ttttaagtac ataaatcaga tttaacacat
     4801 ttattttttg aaaggtacat atacgttctc aatgaagatt tatcctaggt ttcctctctg
     4861 ttcatttcag ttgtagcatg tacgatctag aagctgctgc tcttgctgcc tctgccctcc
     4921 attttggtat gatcatatga ccaaaattcc aatacaactt catgagggtt tttacattaa
     4981 cctgaacaaa taggaaacct tgaatctatt gtgtatgcac atgaacactt gattgtgaat
     5041 aataaaattt gtttttatac agccttcttg gtgaaaagca ttagtgtagt agaccagaaa
     5101 tttcattata gattaatatg gtagcaaaac tttatttatg cttctaaaaa ttattttcag
     5161 gaataaacag tatttgaaga atgggtaaaa acaatgaatg tgtctaggga gagtaatttt
     5221 actgtaagct gaaagtatac atatattttt tatggtaaat gaaattttct tttcttttta
     5281 gttaaacatg ccaagtattg gtgtgtgcat atgctggaaa ttttcatata tatcattttt
     5341 ccatctgagt tatttacact ctccagctta ctttaatatg ttccacatat caacgataca
     5401 gtcaacactt taacatcgtt ttattgtttt gtttatagct ttgcatccat tgtaattcct
     5461 tcataccacc atggtatcct gagtacattt gtgtacattt tgtaagttaa tattttgctt
     5521 aaactgataa gggatggaag gacaattgga gtgactctgc caactattgt cctaagaaat
     5581 gacatcagtc accacttcta atgcattgtc attttttaat ctatttataa ctgctgctca
     5641 cattttagaa aataaggagg tggtgtaaag gtagagaaaa tatgtttaag tcatgctgtt
     5701 ggttaattac ttcaaatatc ttattacctt tagctaggct attttaaatc atgcacatgt
     5761 tgaagaaggg aactgctagt ttgtacatag aagtgatata cataaaaaca ttgaattgct
     5821 tctggtaaac aacgacctac cttaagttat gtttaaagtg tattgactgt gtatttcatg
     5881 gctaatgtca cttgcagagt gtcttaacac caatgaaatt attgttatat gaatcactgt
     5941 tgaactcggc atcccttggc agagccgtgt ttggtttgag ttctacatca ttaaaactat
     6001 atgcatggta acaaattggc cacaaacaca ccctctgact aaaatttgct ttcatgacag
     6061 atatgaatat ttgtgctttc acattgtttt aatgagagta acactcatcc catcaaaatt
     6121 actatattaa atttcacatt ttttctgcaa atactgttga caaaaatagt atagtcttgt
     6181 tattcccaga aacaaaacta tagaaaagat cattttaaaa agcttttaaa ctgtttttcc
     6241 ttagttaatt gtgactatac tcatgtagct cagtgcactt tgtcacgtaa acaaaggcaa
     6301 aacaagtcct gcattggtgt tgagtagcag aaaagaattc ttgcataaat gatttcatct
     6361 tttcttcatt tgaacaacca gcattactgc caaacaccaa gcgtggtcca tatttgtacc
     6421 aggtttttaa catgacatag ttagcaagtt tgattgaaga gatttttagg tcctgggcct
     6481 acaagatttt agtatggtct tttttcatgt ttctaatgct tgaagcatta ttgcttacct
     6541 tgggaaacac agcgttcctg ccatttgtaa gttttcctat atatacgatt ccttttatta
     6601 actgtaaacc tggccttaca gggttcaaat aggcagtatc ccttttaagt gacctttgca
     6661 acccaagtgt tacttgctta tttgatgctc tctgtacctt ctatctcatt gcaatgcctg
     6721 tcttggagtc accaaagctt aaattctact tcttaattta aaactaaagg catagagtct
     6781 aacctagcga gacacaatga attttgtgat ttttttttcc cccacttgcc ccaaattcca
     6841 aatattttat cattagctgg aacttgagtt gttttccatg gcagaatgtt gatattgtca
     6901 atattccgta tacatttctg taaagagcaa atcactaaaa cagaactatc aggtgatgaa
     6961 aatgtccaga attttgaaga aatagatagc tatcaccggt ggtttcatct accttggttt
     7021 tagttcacat taaaaagaac catggaaatt ccactgagac tcttggcatg ctgtcctgtg
     7081 tagatgccaa ctgagtttcc aatgtgacct ggcaaagctg tccttttctt gtgcagtatg
     7141 actgtgggat cgtgttttag ggattctttt catgaaatgc tcattgtgca tcaagcaaaa
     7201 cgccaactgg cttttgtgaa caacactttt ttgttttgtt ttgttttatt tttcctcccc
     7261 ttattatgtt ctctggacac ttttgtggaa attaactagt cggatgaaca cattttgtaa
     7321 aaatttgtat aatactgata taaaaaaaat atttataatc ccagaaaatg ttgtgttaaa
     7381 tcttgtgcaa aaacagtata ttcagaataa aagtttatca ttgaaagtca ccacac
//
