LOCUS       NM_029862               1648 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus calcium channel flower domain containing 1 (Cacfd1),
            transcript variant 1, mRNA.
ACCESSION   NM_029862 XM_355322
VERSION     NM_029862.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Li,C., Zhang,M., Du,Y., Liu,S., Li,D., Zhang,S., Ji,F., Zhang,J.
            and Jiao,J.
  TITLE     Compromised cell competition exhausts neural stem cells pool
  JOURNAL   Cell Prolif 57 (12), e13710 (2024)
   PUBMED   39010274
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Chang,H.F., Mannebach,S., Beck,A., Ravichandran,K., Krause,E.,
            Frohnweiler,K., Fecher-Trost,C., Schirra,C., Pattu,V., Flockerzi,V.
            and Rettig,J.
  TITLE     Cytotoxic granule endocytosis depends on the Flower protein
  JOURNAL   J Cell Biol 217 (2), 667-683 (2018)
   PUBMED   29288152
  REMARK    GeneRIF: The Flower protein mutant lacking binding sites for the
            endocytic adaptor AP-2 proteins fails to rescue endocytosis,
            indicating that Flower protein interacts with proteins of the
            endocytic machinery to mediate granule endocytosis. Thus, our data
            identify Flower protein as a key protein mediating granule
            endocytosis.
REFERENCE   3  (bases 1 to 1648)
  AUTHORS   Gineste,C., De Winter,J.M., Kohl,C., Witt,C.C., Giannesini,B.,
            Brohm,K., Le Fur,Y., Gretz,N., Vilmen,C., Pecchi,E., Jubeau,M.,
            Cozzone,P.J., Stienen,G.J., Granzier,H., Labeit,S.,
            Ottenheijm,C.A., Bendahan,D. and Gondin,J.
  TITLE     In vivo and in vitro investigations of heterozygous nebulin
            knock-out mice disclose a mild skeletal muscle phenotype
  JOURNAL   Neuromuscul Disord 23 (4), 357-369 (2013)
   PUBMED   23375831
REFERENCE   4  (bases 1 to 1648)
  AUTHORS   Petrova,E., Lopez-Gay,J.M., Rhiner,C. and Moreno,E.
  TITLE     Flower-deficient mice have reduced susceptibility to skin papilloma
            formation
  JOURNAL   Dis Model Mech 5 (4), 553-561 (2012)
   PUBMED   22362363
  REMARK    GeneRIF: Skin papilloma cells take advantage of mFwe activity to
            facilitate their clonal expansion.
REFERENCE   5  (bases 1 to 1648)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845266.2 and AK052562.1.
            
            On Aug 4, 2011 this sequence version replaced NM_029862.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK052562.1, BB660613.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                AL845266.2         30792-30831
            41-1648             AK052562.1         2-1609
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 19.17 cM"
     gene            1..1648
                     /gene="Cacfd1"
                     /gene_synonym="5930434B04Rik; mFwe"
                     /note="calcium channel flower domain containing 1"
                     /db_xref="GeneID:381356"
                     /db_xref="MGI:MGI:1924317"
     exon            1..180
                     /gene="Cacfd1"
                     /gene_synonym="5930434B04Rik; mFwe"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..578
                     /gene="Cacfd1"
                     /gene_synonym="5930434B04Rik; mFwe"
                     /note="isoform 1 is encoded by transcript variant 1;
                     calcium channel flower homolog; likely ortholog of H.
                     sapiens chromosome 9 open reading frame 7 (C9orf7);
                     calcium channel flower domain-containing protein 1"
                     /codon_start=1
                     /product="calcium channel flower homolog isoform 1"
                     /protein_id="NP_084138.1"
                     /db_xref="CCDS:CCDS38086.1"
                     /db_xref="GeneID:381356"
                     /db_xref="MGI:MGI:1924317"
                     /translation="MSGSGAAGAAAGPAPPAQEEGMTWWYRWLCRLAGVLGAVSCAIS
                     GLFNCVTIHPLNIAAGVWMIMNAFILLLCEAPFCCQFVEFANTVAEKVDRLRSWQKAV
                     FYCGMAIVPIVMSLTLTTLLGNAIAFATGVLYGLSALGKKGDAISYARIQQQRQQADE
                     EKLAETFEGEL"
     exon            181..253
                     /gene="Cacfd1"
                     /gene_synonym="5930434B04Rik; mFwe"
                     /inference="alignment:Splign:2.1.0"
     exon            254..379
                     /gene="Cacfd1"
                     /gene_synonym="5930434B04Rik; mFwe"
                     /inference="alignment:Splign:2.1.0"
     exon            380..487
                     /gene="Cacfd1"
                     /gene_synonym="5930434B04Rik; mFwe"
                     /inference="alignment:Splign:2.1.0"
     exon            488..579
                     /gene="Cacfd1"
                     /gene_synonym="5930434B04Rik; mFwe"
                     /inference="alignment:Splign:2.1.0"
     exon            580..1648
                     /gene="Cacfd1"
                     /gene_synonym="5930434B04Rik; mFwe"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1248
                     /gene="Cacfd1"
                     /gene_synonym="5930434B04Rik; mFwe"
                     /note="major polyA site"
ORIGIN      
        1 aaggccgcgc gcgggtggac acgtctcttt gtgcgcgcct ggggctgcgc gcagcgttta
       61 gcatgagcgg ctcgggcgcc gccggagcgg ccgcgggccc agccccgccg gcacaggaag
      121 agggcatgac ttggtggtac cgctggctgt gccgcctggc gggagtgcta ggagcggtgt
      181 cctgtgccat ctctggactc ttcaactgcg tcactatcca ccctctgaac atcgcagctg
      241 gcgtgtggat gatcatgaac gccttcatcc tgttgttgtg cgaggctccc ttctgctgcc
      301 agtttgtgga gtttgcaaac acagtagctg agaaggttga ccggctgcgc tcctggcaga
      361 aggctgtctt ctactgcggg atggccatcg tccccatcgt catgagcctc accctgacta
      421 cactgctggg caatgccatc gcctttgcca cgggggtgct gtatggactg tctgccctgg
      481 gcaaaaaggg cgatgccatt tcttatgctc ggatccagca gcagagacag caggcagatg
      541 aggagaagct ggcggagact ttcgaggggg aactgtgagc ctggagcctt tggagagctg
      601 ggcagagacc agacccaccc tacacagact tggccccttc tcgggtacca gagcacagag
      661 agtaagcctg catggttggg acaaggagag ctgtttcact ggcacatggc tccagggaca
      721 tgccttgctg tcccctcacc tttctctgca ctgttctctt cccactgtcc tgtgacaccg
      781 gcatcagtgg cttattagat gagtccttgg ccattaggca catttggtgg cctggctgaa
      841 gatgatgtgt caagtgctga gccctgcctc cctcctgcac tagaccttca gccggacctg
      901 tccatggaac ccagccctgg agtcactgag tctccaggga atccttagct tctcagggct
      961 tgaggcccag aggagtagac attcattctc cttggtatag aagctctgat ctgtggactt
     1021 tggggatagc agaagttctc cagagagcct ggccatgctg aactggggag tggcagaaat
     1081 gtaatagtgt agctagctta gggggtcatg tgccttcacc gttcatcaga ggctattttt
     1141 cttgagccag agataagttt caagggctct gtgtatgtgt gtctgtatat gttggagcct
     1201 atgttctcag taagacctgt ctacctccca ggaggagaaa aatccccacc tgctcctctg
     1261 gttacttggg taggcatgga cttgggccag ttctgcccag gcctcctgcc tccacagtag
     1321 aattcaaagg ctacatgtat ggtgagattg tacttcacta gggaatccat tttgtgctag
     1381 gcagccatgg ggtgaggtat aaaggtggag gccctcagat ataggccaat ttggggtggt
     1441 cagtcactta agtcctgagg ttcttggcag tcccatggca cagtggctct ggagcccatt
     1501 gctttgactc tgaccatggg cactgtccag cccagactag gcctcattct tgtctgtgtc
     1561 tgggtgtctt ctgtggctcc ttttgcaccc acccctggtt tgtttgttga ctgggttact
     1621 tttggagtaa agcaagtttg tagttggt
//
